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THAILAND JOURNAL OF HEALTH PROMOTION AND ENVIRONMENTAL HEALTH

October - December 2012

121

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Effect of Folic Acid on Proliferation of Human Dental Pulp Cells Kulwarat Sirinuntakate Montatip Klatkruamat Worawanit Suwannaboon Apichat Nakkaya Udom Sasikornwonge Rungarun Kriangkrai Faculty of Dentistry, Naresuan University

Dental student Dental student Dental student Dental student Dental student Lecturer

Abstract Stem cells have special properties including high proliferative properties to produce more stem cells and unspecialized function but they have the capacity to differentiate into specialized cells that can carry out these functions. Adult stem cells are routinely used in medical therapies but collecting of stem cells is invasive and painful procedure. Recently, human dental pulp cells represent an advantageous source of stem cells with wholly non-invasive procedure of collecting.

Thus, research studies to promote the stem cell properties of dental pulp cells using for regenerative therapies are still needed. Folic acid is essential for craniofacial development. Recently, folic acid can promote a proliferation of embryonic neural stem cells. However, effect of folic acid on human dental pulp cells has never been reported. The purpose of this study was to investigate an in vitro effect of folic acid on human dental pulp cells. Human dental pulp cells were treated with 1, 0.1, and 0.01 mM folic acid for 24 hours and MTT assay was used to measure the cell viability.

It was found that folic acid significantly promoted the cell viability of human dental pulp cells resulting in increased cell number. Our results suggested that folic acid promotes cell proliferation and provide for further studies to investigate underlying mechanisms of folic acid on human dental pulp cells.

Keywords: Folic acid, Human dental pulp cells, Cell viability, Cell proliferation

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°≈ÿà¡¢Õß«‘µ“¡‘π∫’√«¡ ´÷Ëßæ∫‰¥â„πº—°„∫‡¢’¬« µ—∫ ¬’ µå ¢π¡ªíß ·≈–º≈‰¡â ¥6 °√¥‚ø≈‘°¡’§«“¡ ”§—≠µàÕ°√–∫«π°“√ —߇§√“–À奒‡Õπ‡Õ (DNA synthesis) ´÷Ë ß ¡’ § «“¡ ”§— ≠ µà Õ °√–∫«π°“√ ·∫àßµ—«¢Õ߇´≈≈å·≈–°√–∫«π°“√‡µ‘¡À¡Ÿà‡¡∑‘≈ (Methylation cycle) ´÷Ë ß ¡’ § «“¡®”‡ªì π µà Õ °√–∫«π°“√æ—≤π“¢Õßµ—«ÕàÕπ (Embryogenesis)7,8 °“√»÷°…“„πÀâÕߪؑ∫—µ‘°“√„πªí®®ÿ∫—πæ∫ «à “ °√¥‚ø≈‘ ° à ß ‡ √‘ ¡ °“√·∫à ß µ— « ¢Õ߇´≈≈å µâ π °”‡π‘¥¢Õß√–∫∫ª√– “∑„πµ—«ÕàÕπ (Embryonic neural stem cell)9 ‡´≈≈åµâπ°”‡π‘¥¢Õß√–∫∫ ª√– “∑π’È ¡’ µâ π °”‡π‘ ¥ ¡“®“°‡π◊È Õ ‡¬◊Ë Õ √–∫∫ ª√– “∑∑’Ë ‡ ªì π µâ π °”‡π‘ ¥ ‡¥’ ¬ «°— π °— ∫ °≈ÿà ¡ ‡´≈≈å ¢Õ߇¥Áπ‡∑≈¡’‡´π‰§¡å∑’ˇ®√‘≠‰ª‡ªìπ‡´≈≈å„π‚æ√ß ª√– “∑øíπ´÷ßË ¡’§≥ ÿ ¡∫—µ‡‘ ªìπ‡´≈≈åµπâ °”‡π‘¥ ‡™à𠇥’¬«°—π Õ¬à“߉√°Áµ“¡º≈¢Õß°√¥‚ø≈‘°µàÕ°“√ ‡®√‘≠¢Õ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å¬—ß ‰¡à¡’√“¬ß“π°“√»÷°…“¡“°àÕπ °≈ÿà¡«‘®—¬®÷ß¡’§«“¡ π„®∑’Ë®–»÷°…“º≈¢Õß°√¥‚ø≈‘°µàÕ°“√‡®√‘≠¢Õß ‡´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å ‚¥¬º≈¢Õß °“√»÷°…“π’È®–‡ªìπ¢âÕ¡Ÿ≈æ◊Èπ∞“π„π°“√«‘®—¬‡æ◊ËÕ æ— ≤ π“°“√‡æ“–‡≈’È ¬ ߇´≈≈å µâ π °”‡π‘ ¥ ®“°‚æ√ß ª√– “∑øíπ¢Õß¡πÿ…¬åµàÕ‰ª

«—µ∂ÿª√– ߧ尓√»÷°…“ ‡æ◊ËÕ∑¥ Õ∫º≈¢Õß°√¥‚ø≈‘°µàÕ°“√·∫àß µ—«¢Õ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å «—¥º≈ °“√»÷ ° …“®“°§«“¡¡’ ™’ «‘ µ ¢Õ߇´≈≈å ≈å „ π‚æ√ß ª√– “∑øíπ¢Õß¡πÿ…¬å∑’Ë∂Ÿ°∑¥ Õ∫¥â«¬°√¥‚ø≈‘° ∑’Ë¡’§«“¡‡¢â¡¢âπ·µ°µà“ß°—π ‚¥¬‡∑§π‘§ MTT assay „πÀâÕߪؑ∫—µ‘°“√

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THAILAND JOURNAL OF HEALTH PROMOTION AND ENVIRONMENTAL HEALTH

October - December 2012

123

∫ ∑ «‘ ∑ ¬ “ ° “ √ 1. °√¥‚ø≈‘° (C19H19N7O6) ®“°∫√‘…—∑  Sigma ∂Ÿ°≈–≈“¬„πÕ“À“√‡≈’Ȭ߇´≈≈å∑’ˉ¡à¡’´’√—¡ ·≈â«∑¥ Õ∫º≈∑’Ë¡’µàÕ‡´≈≈å„π‚æ√ߪ√– “∑øíπ ¢Õß¡πÿ…¬å„π√–¥—∫§«“¡‡¢â¡¢âπ 1 0.1 ·≈– 0.01 ¡‘≈≈‘‚¡≈“√å ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß 2. Õ“À“√‡≈’Ȭ߇´≈≈å„π‚æ√ߪ√– “∑øíπ ‰¥â·°à Dulbeccoûs Modified Eagleûs Medium (DMEM) ®“°∫√‘…—∑ Gibco ∑’Ë„ à´’√—¡ (Fetal calf serum) ®“°∫√‘…—∑ Gibco √âÕ¬≈– 10 ·≈– „ ଓªÆ‘™’«π– ‰¥â·°à ‡æπ‘´‘≈≈‘π/ ‡µ√ª‚µ¡—¬´‘π ´—≈‡øµ·≈–·Õ¡‚ø‡∑Õ√‘´π‘ ∫’ (Penicillin/Streptomycin sulfate and Amphotericin B ) ®“° ∫√‘…—∑ Gibco √âÕ¬≈– 1 °“√‡æ“–‡≈’Ȭ߇´≈≈å„π‚æ√ߪ√– “∑ øíπ¢Õß¡πÿ…¬å ‡´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å‰¥â¡“ ®“°‡π◊ÈÕ‡¬◊ËÕ„π‚æ√ߪ√– “∑øíπ¢ÕßøíπºŸâªÉ«¬∑’Ë¡’ ÿ¢¿“æ·¢Áß·√ß ‰¡à¡’‚√§ª√–®”µ—« ¡’Õ“¬ÿ 18-25 ªï ·≈–‡¢â“√—∫°“√√—°…“‚¥¬°“√∂Õπøíπ°√“¡·∑â´∑’Ë ’Ë 3 ‡π◊ËÕß®“°‡ªìπøíπ§ÿ¥∑’˧≥–∑—πµ·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬π‡√»«√·≈–‰¥â√—∫§«“¡¬‘π¬Õ¡®“°ºŸâ ªÉ « ¬„Àâ „ ™â øí π ¥— ß °≈à “ «‡æ◊Ë Õ °“√¥”‡π‘ π ß“π«‘ ®— (‚§√ß°“√«‘ ®— ¬ ‰¥â ºà “ π°“√√— ∫ √Õß®“°§≥– °√√¡°“√®√‘ ¬ ∏√√¡‡°’Ë ¬ «°— ∫ °“√«‘ ®— ¬ „π¡πÿ … ¬å ¡À“«‘∑¬“≈—¬π‡√»«√ √À— ‚§√ß°“√∑’ˉ¥â√—∫°“√ √— ∫ √Õߧ◊ Õ 5401010027) ®“°π—È π „™â ≈‘Ë ¡ µÕ° ∫√‘ ‡ «≥ßà “ ¡√“°øí π ‡æ◊Ë Õ π”‡π◊È Õ ‡¬◊Ë Õ „π‚æ√ß ª√– “∑øíπÕÕ°¡“·≈â«≈â“ߥ⫬ Phosphate Buffer Saline (PBS) ∑’˪√“»®“°‡™◊ÈÕ π”‡π◊ÈÕ‡¬◊ËÕ‚æ√ß ª√– “∑øíπ¡“µ—¥‡ªìπ™‘πÈ ‡≈Á°Ê¢π“¥ª√–¡“≥ 1 ≈Ÿ° ∫“»°å¡≈‘ ≈‘‡¡µ√ ·≈–«“ß„π®“π‡≈’¬È ߇´≈≈å (Nunc) ·≈–‡≈’Ȭ߄πÕ“À“√‡≈’Ȭ߇´≈≈å™π‘¥ Dulbeccoûs Modified Eagleûs Medium (DMEM), Gibco ∑’˪√–°Õ∫¥â«¬´’√—¡√âÕ¬≈– 10 (10% Fetal calf 124 µÿ≈“§¡ - ∏—𫓧¡ 2555 °√¡Õπ“¡—¬

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serum, Gibco) ‡æπ‘´‘≈≈‘π/ ‡µ√ª‚µ¡—¬´‘π ´—≈‡øµ ·≈–·Õ¡‚ø‡∑Õ√‘´π‘ ∫’√Õâ ¬≈– 1 (1% Penicillin/Streptomy cinsulfate and Amphotericin B, Gibco) ·≈–∂Ÿ°‡≈’¬È ß„πµŸÕâ ∫∑’ÕË ≥ ÿ À¿Ÿ¡‘ 37 Õß»“‡´≈‡´’¬ ·≈–¡’√–¥—∫§“√å∫Õπ‰¥ÕÕ°‰´¥å √âÕ¬≈– 5 ‡´≈≈剥⇧≈◊ËÕπÕÕ°®“°™‘Èπ‡π◊ÈÕ·≈– ‡®√‘≠®π‡µÁ¡®“π‡≈’Ȭ߇´≈≈å ª√–¡“≥ 2 —ª¥“Àå (√Ÿª∑’Ë 1) ®“°π—πÈ ‰¥â∂“à ¬≈ß®“π‡≈’¬È ߇´≈≈å„À¡à ·≈– ‡√‘¡Ë π—∫‡ªìπ‡´≈≈å√πÿà ∑’Ë 1 ‚¥¬°“√»÷°…“π’„È ™â‡´≈≈å√πÿà ∑’Ë 4 ®“°ºŸâªÉ«¬®”π«π 2 √“°“√ √â“ß°√“ø¡“µ√∞“π À«à“π‡´≈≈å∑’Ë∑√“∫®”π«π≈ß„π®“π‡æ“– ‡≈’¬È ß·∫∫ 24 À≈ÿ¡ ®”π«π 20,000 40,000 60,000 ·≈– 80,000 ‡´≈≈åµàÕÀ≈ÿ¡ ·≈â«π”‡¢â“µŸâÕ∫‡æ“– ‡≈’È ¬ ߇´≈≈å ‡ ªì 𠇫≈“ 18 ™—Ë « ‚¡ß À≈— ß ®“°π—È π ∑”°“√«—¥®”π«π‡´≈≈å¥â«¬‡∑§π‘§ MTT assay ·≈â«π”º≈¡“À“§«“¡ —¡æ—π∏凪ìπ°√“ø‡ âπµ√ß · ¥ß§«“¡ —¡æ—π∏å¢Õß®”π«π‡´≈≈å°—∫§à“°“√¥Ÿ¥ °≈◊π· ß∑’Ë¡’§à“ —¡ª√– ‘∑∏å‘ À —¡æ—π∏å (R2) ‡¢â“ „°≈â 1 °“√«—¥º≈¢Õß°√¥‚ø≈‘°µàÕ§«“¡¡’ ™’«‘µ¢Õ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å ‚¥¬‡∑§π‘§ MTT assay °“√«— ¥ º≈‚¥¬‡∑§π‘ § MTT assay (ª√—∫ª√ÿß¡“®“° Mosmann, 1983)10 ‡´≈≈å ‡æ“–‡≈’Ȭ߉¥â∂Ÿ°∂à“¬≈ß„π®“π‡≈’Ȭ߇´≈≈å·∫∫ 24 À≈ÿ¡ ®”π«π 50,000 ‡´≈≈åµàÕÀ≈ÿ¡ ∑”°“√‡≈’È¬ß ‡´≈≈å„πÕ“À“√‡≈’Ȭ߇´≈≈媰µ‘‡ªìπ‡«≈“ 18 ™—Ë«‚¡ß ®“°π—Èπ‡ª≈’ˬπ‡ªìπÕ“À“√‡≈’Ȭ߇´≈≈媰µ‘∑’ˉ¡à¡’ ´’√—¡·≈–π”‡¢â“µŸâÕ∫‡æ“–‡≈’Ȭ߇´≈≈åµàÕ‡ªìπ‡«≈“ 6 ™—Ë«‚¡ß µàÕ¡“‡´≈≈剥â∂Ÿ°∑¥ Õ∫¥â«¬°√¥‚ø≈‘°∑’Ë √–¥—∫§«“¡‡¢â¡¢âπ∑’Ë 1 0.1 ·≈– 0.01 ¡‘≈≈‘‚¡≈“√å ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß °≈ÿࡧ«∫§ÿ¡®–‡≈’Ȭ߇´≈≈å„π ¿“«–‡¥’¬«°—∫°≈ÿ¡à ∑¥≈Õß·µàª√“»®“°°√¥‚ø≈‘°

º≈¢Õß°√¥‚ø≈‘°µàÕ°“√·∫àßµ—«¢Õ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å

®“°π—πÈ „ à MTT ∑’§Ë «“¡‡¢â¡¢âπ ÿ¥∑⓬‡∑à“°—∫ 0.5 ¡‘≈≈‘°√—¡µàÕ¡‘≈≈‘≈‘µ√ ∫à¡„πµŸâ∑’ËÕÿ≥À¿Ÿ¡‘ 37 Õß»“ ‡´≈‡´’¬ ‡ªìπ‡«≈“ 20 π“∑’ ¥ÿ ∑⓬¢Õß°“√∑¥ Õ∫ À≈—ß®“°π—πÈ ≈–≈“¬º≈÷°øÕ√å¡“´“π (Formazan) ∑’Ë ‡´≈≈å √â“ߢ÷Èπ®“° “√≈–≈“¬ MTT „π·µà≈–À≈ÿ¡ ¢Õß®“π‡≈’È ¬ ß‚¥¬„™â Dimethyl sulfoxide (DMSO) ·≈â«π”‰ªÕà“π§à“°“√¥Ÿ¥°≈◊π· ߥ⫇§√◊ËÕ߉¡‚§√‡æ≈∑√’‡¥Õ√å (Microplate reader) (BIO-RAD √ÿπà xMark) ∑’§Ë «“¡¬“«§≈◊πË · ß 570 π“‚π‡¡µ√ √à«¡°—∫§«“¡¬“«§≈◊Ëπ· ß 630 π“‚π ‡¡µ√ π”§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë«—¥‰¥â¡“‡∑’¬∫§à“ ®”π«π‡´≈≈å∑’ˬ—ß¡’™’«‘µÕ¬Ÿà°—∫‡ âπ°√“ø¡“µ√∞“π ‚¥¬„π·µà≈–°≈ÿà¡°“√∑¥≈Õ߉¥â∑”´È”°—π 3 §√—Èß · ¥ßº≈¢Õß®”π«π‡´≈≈å„π·µà≈–°≈ÿà¡°“√∑¥≈Õß

®“°√âÕ¬≈–§«“¡¡’™«’ µ‘ ¢Õ߇´≈≈å (% Cell viability) ‚¥¬„π·µà≈–°“√∑¥≈Õß¡’§à“‡∑à“°—∫ (®”π«π ‡´≈≈å∑’ˬ—ß¡’™’«‘µÕ¬Ÿà„π·µà≈–°≈ÿà¡∑¥≈ÕßµàÕ®”π«π ‡´≈≈å∑’Ë¡’™’«‘µ„π°≈ÿࡧ«∫§ÿ¡) §Ÿ≥¥â«¬ 100 °“√«‘‡§√“–Àå¢âÕ¡Ÿ≈ °“√«‘ ‡ §√“–Àå ¢â Õ ¡Ÿ ≈ „™â ∂‘ µ‘ ‡ ™‘ ß æ√√≥π“ ‰¥â·°à °“√®—¥·π«‚π⡇¢â“ Ÿà à«π°≈“ß (§à“‡©≈’ˬ) ·≈–°“√«—¥°“√°√–®“¬ ( à«π‡∫’ˬ߇∫π¡“µ√∞“π) ¢Õß√âÕ¬≈–¢Õߧ«“¡¡’™’«‘µ¢Õ߇´≈≈å„π·µà≈–°≈ÿà¡ ∑¥≈Õ߇ª√’¬∫‡∑’¬∫°—∫°≈ÿࡧ«∫§ÿ¡ «‘‡§√“–Àå §«“¡·µ°µà“ߢÕß§à“‡©≈’ˬ¢Õß√âÕ¬≈–¢Õߧ«“¡¡’ ™’«‘µ¢Õ߇´≈≈å„π·µà≈–°≈ÿà¡∑¥≈Õ߇ª√’¬∫‡∑’¬∫°—∫ °≈ÿࡧ«∫§ÿ¡‚¥¬Õ“»—¬ ∂‘µ‘§«“¡·ª√ª√«π·∫∫ ªí®®—¬‡¥’¬« (One-way ANOVA)

√Ÿª∑’Ë 1 · ¥ß°“√‡æ“–‡≈’Ȭ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å ‚¥¬π”‡π◊ÈÕ‡¬◊ËÕ‚æ√ߪ√– “∑øíπ µ—¥‡ªìπ™‘Èπ‡≈Á°Ê ¢π“¥ª√–¡“≥ 1 ≈Ÿ°∫“»°å¡‘≈≈‘‡¡µ√ (A) ·≈–«“ß„π®“π‡≈’Ȭ߇´≈≈å ·≈–‡≈’Ȭ߄πÕ“À“√‡≈’Ȭ߇´≈≈å (B) „πµŸâÕ∫∑’ËÕÿ≥À¿Ÿ¡‘ 37 Õß»“‡´≈‡´’¬ ·≈–¡’√–¥—∫ §“√å∫Õπ‰¥ÕÕ°‰´¥å√âÕ¬≈– 5 (C) ‡´≈≈剥⇧≈◊ËÕπÕÕ°®“°™‘Èπ‡π◊ÈÕ·≈–‡®√‘≠∫π®“π ‡≈’Ȭ߇´≈≈å (D) ‡æ◊ËÕ„™â„π°“√»÷°…“µàÕ‰ª (E) §◊Õ ‡π◊ÈÕ‡¬◊ËÕ‚æ√ߪ√– “∑øíπ∫π®“π‡≈’È¬ß ‡´≈≈å

º≈°“√»÷°…“ º≈»÷°…“æ∫«à“°≈ÿࡧ«∫§ÿ¡¡’§à“‡©≈’ˬ√âÕ≈–§«“¡¡’™’«‘µ¢Õ߇´≈≈å¡’§à“‡∑à“°—∫ 100 ·≈–‡¡◊ËÕ ‡´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å ‰¥â√—∫°√¥ ‚ø≈‘°∑’Ë√–¥—∫§«“¡‡¢â¡¢âπ 1 0.1 ·≈– 0.01 ¡‘≈≈‘ ‚¡≈“√å ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß æ∫§à“‡©≈’ˬ√âÕ¬≈– §«“¡¡’ ™’ «‘ µ ¢Õ߇´≈≈å ¡’ §à “ ‡∑à “ °— ∫ 172.0964

126.7459 ·≈– 148.8372 µ“¡≈”¥—∫ ·≈–‡¡◊ËÕπ” º≈°“√»÷°…“¡“«‘‡§√“–Àå∑“ß ∂‘µ‘ æ∫«à“¢âÕ¡Ÿ≈¡’ °“√·®°·®ß·∫∫ª°µ‘ · ≈–æ∫«à “ ‡´≈≈å „ π‚æ√ß ª√– “∑øíπ¢Õß¡πÿ…¬å∑’ˉ¥â√—∫°√¥‚ø≈‘°„π·µà≈– §«“¡‡¢â¡¢âπ¡’§à“‡©≈’ˬ√âÕ¬≈–§«“¡¡’™’«‘µ¢Õ߇´≈≈å ·µ°µà“ß®“°°≈ÿࡧ«∫§ÿ¡Õ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘µ‘ ∑’Ë√–¥—∫§«“¡‡™◊ËÕ¡—Ëπ√âÕ¬≈– 95 (p<0.05) (√Ÿª∑’Ë 3)

THAILAND JOURNAL OF HEALTH PROMOTION AND ENVIRONMENTAL HEALTH

October - December 2012

125

∫ ∑ «‘ ∑ ¬ “ ° “ √ √Ÿª∑’Ë 2 · ¥ß°√“ø¡“µ√∞“π· ¥ß§«“¡ —¡æ—π∏å√–À«à“ß§à“°“√¥Ÿ¥°≈◊π· ß (Absorbance) ·≈– ®”π«π‡´≈≈å (Cell number) „π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å

®“°√Ÿª∑’Ë 2 · ¥ß°√“ø¡“µ√∞“π‚¥¬·π« ·°π Y · ¥ß®”π«π‡´≈≈å·≈–·π«·°π X · ¥ß §à“°“√¥Ÿ¥°≈◊π· ß∑’§Ë «“¡¬“«§≈◊πË (Absorbance) 570 π“‚π‡¡µ√√à«¡°—∫§«“¡¬“«§≈◊Ëπ 630 π“‚π

‡¡µ√ æ∫§«“¡ —¡æ—π∏å√–À«à“ß®”π«π‡´≈≈å·≈– §à“°“√¥Ÿ¥°≈◊π· ߢÕ߇´≈≈å„π‚æ√ߪ√– “∑øíπ ¢Õß¡πÿ…¬å „π°“√»÷°…“π’È §◊Õ Y = 343500X ·≈– ¡’§à“ —¡ª√– ‘∑∏å‘ À —¡æ—π∏å (R2) ‡∑à“°—∫ 0.985

√Ÿª∑’Ë 3 · ¥ß√âÕ¬≈–§«“¡¡’™’«‘µ¢Õ߇´≈≈å (% Cell viability) „π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å ‡¡◊ËÕ∑¥ Õ∫¥â«¬°√¥‚ø≈‘°∑’Ë√–¥—∫§«“¡‡¢â¡¢âπ 1 0.1 ·≈– 0.01 ¡‘≈≈‘‚¡≈“√凪ìπ‡«≈“ 24 ™—Ë«‚¡ß

*· ¥ß§«“¡·µ°µà“ßÕ¬à“ß¡’π—¬ ”§—≠∑’Ë p < 0.05 ‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫°—∫°≈ÿࡧ«∫§ÿ¡ (ANOVA test)

126 µÿ≈“§¡ - ∏—𫓧¡ 2555 °√¡Õπ“¡—¬

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º≈¢Õß°√¥‚ø≈‘°µàÕ°“√·∫àßµ—«¢Õ߇´≈≈å„π‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å

√ÿª·≈–«‘®“√≥åº≈ ®“°º≈°“√»÷°…“ Õ¥§≈âÕ߉ª°—∫º≈°“√ »÷ ° …“„πÀâ Õ ßªØ‘ ∫— µ‘ ° “√∑’Ë ºà “ π¡“∑’Ë · ¥ß∂÷ ß ª√–‚¬™πå¢Õß°√¥‚ø≈‘° ‚¥¬¡’§«“¡ Õ¥§≈âÕß ¢Õß√–¥—∫§«“¡‡¢â¡¢âπ¢Õß°√¥‚ø≈‘°µàÕ°“√µÕ∫ πÕßµàÕ‡´≈≈åÀ√◊Õ‡π◊ÈÕ‡¬◊ËÕ∑’ˉ¥â√—∫ ‚¥¬°√¥‚ø≈‘° ∑’Ë√–¥—∫§«“¡‡¢â¡¢âπ 1 0.1 ·≈– 0.01 ¡‘≈≈‘‚¡≈“√å “¡“√∂≈¥§«“¡æ‘°“√¢Õßµ—«ÕàÕπ®“°°“√‡Àπ’ˬ« π”¢Õß°√¥‡√∑‘‚πÕ‘°·≈–‡Õ∑‘≈·Õ≈°ÕŒÕ≈剥â11, 12 ·≈–°√¥‚ø≈‘°∑’Ë√–¥—∫ 0.1 ·≈– 0.01 ¡‘≈≈‘‚¡≈“√å “¡“√∂°√–µÿâπ°“√·∫àßµ—« (Proliferation) ·≈– ≈¥°“√µ“¬·∫∫Õ–æÕæ‚∑´‘ (Apoptosis) ¢Õß ‡´≈≈åµπâ °”‡π‘¥¢Õß√–∫∫ª√– “∑„πµ—«ÕàÕπ‰¥â 9, 13 ®“°°“√»÷°…“∂÷ß —≠≠“≥π”‡¢â“ Ÿà‡´≈≈å¢Õß°√¥ ‚ø≈‘°æ∫«à“°√¥‚ø≈‘°°√–µÿâπ°“√· ¥ßÕÕ°¢Õß ‚ª√µ’ π ∑’Ë ¡’ § «“¡®”‡ªì π µà Õ °√–∫«π°“√µ‘ ¥ µà Õ ◊ÕË “√√–À«à“߇´≈≈å (Cell-cell communication) à ß ‡ √‘ ¡ „Àâ ‡ °‘ ¥ °“√·∫à ß µ— « ¢Õ߇´≈≈å · ≈–≈¥°“√ µ“¬¢Õ߇´≈≈å · ∫∫Õ–æÕæ‚∑´‘ ¢Õ߇´≈≈å µâ π °”‡π‘¥¢Õß√–∫∫ª√– “∑„πµ—«ÕàÕπ πÕ°®“°π’È °√¥‚ø≈‘°¬—ß à߇ √‘¡„À⇴≈≈å§ß§«“¡‡ªìπ‡´≈≈åµâπ °”‡π‘¥¢Õß√–∫∫ª√– “∑Õ’°¥â«¬9,14,15 „πÕ’°∑“ß Àπ÷Ë ß ‡´≈≈å „ π‚æ√ߪ√– “∑øí π ¢Õß¡πÿ … ¬å ¡’ §ÿ≥ ¡∫—µ‘‡ªìπ‡´≈≈åµâ𰔇𑥇™àπ°—π Àπ÷Ëß„π §ÿ ≥ ¡∫— µ‘ ∑’Ë ”§— ≠ §◊ Õ ¡’ § «“¡ “¡“√∂ Ÿ ß „π°“√ ·∫àßµ—« º≈°“√»÷°…“π’È· ¥ß„Àâ‡ÀÁπ∂÷ߪ√–‚¬™πå

¢Õß°√¥‚ø≈‘ ° „π°“√ à ß ‡ √‘ ¡ °“√·∫à ß µ— « ¢Õß ‡´≈≈å‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å‚¥¬æ∫«à“√âÕ≈–§«“¡¡’ ™’ «‘ µ ¢Õ߇´≈≈å ∑’Ë ∂Ÿ ° ∑¥ Õ∫¥â « ¬°√¥ ‚ø≈‘°®–¡’ª√‘¡“≥ Ÿß°«à“°≈ÿࡧ«∫§ÿ¡∑’ˉ¡à‰¥â∂Ÿ° ∑¥ Õ∫¥â«¬°√¥‚ø≈‘°Õ¬à“ß¡’π—¬ ”§—≠ √ÿª‰¥â«à“ °√¥‚ø≈‘° “¡“√∂°√–µÿâπ°“√‡æ‘Ë¡®”π«π‡´≈≈å„π ‚æ√ߪ√– “∑øíπ¢Õß¡πÿ…¬å„πÀâÕߪؑ∫—µ‘°“√‚¥à߇ √‘¡°“√·∫àßµ—«¢Õ߇´≈≈å„π∑ÿ°√–¥—∫§«“¡‡¢â¡ ¢âπ∑’Ë∑¥ Õ∫

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