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Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique
8.3.3 For Liquid Samples:
8.3.3.1 Prepare a whirl-bag for each sample by labeling with sample identification and test type.
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8.3.3.2 After preparing the work space as outlined in section 8.2, sanitize hands and don gloves.
8.3.3.3 Open the pre-labeled sample whirl-bag and place in a plastic basket.
8.3.3.4 Next, open the sample bag or sample container.
8.3.3.5 Open a sterile box of pipette tips and slowly pipette the appropriate volume into the whirl-bag.
8.3.3.5.1 Never place the pipette with the tip attached on the counter.
8 3 3 6 After transfer, record the volumes on the appropriate LFs, close whirl bag and sample container
8 3 3 7 Eject pipette tip in autoclave bag and repeat with remaining samples
8 3 4 To transfer samples to other bags:
8 3 4 1 Prepare a new whirl-bag for each sample by labeling with sample identification and test type
8 3 4 2 After preparing the work space as outlined in section 8 2, sanitize hands and don gloves
8 3 4 3 Open the second pre-labeled sample whirl-bag
8 3 4 4 Place a plastic basket on the scale, then place the pre-labeled whirl-bag in the plastic bag and tare the scale
8 3 4 5 Next, open the original whirl-bag containing the sample
8.3.4.6 Open a sterile plastic spoon and scoop the appropriate amount of sample into the whirl-bag on the scale as indicated by the test method. Or, open a sterile box of pipette tips and slowly pipette the appropriate volume into the whirl-bag.
8.3.4.6.1 Never place the spoon or the pipette with the tip attached on the counter.
8.3.4.7 After scaling, record the weights on the appropriate LFs, close whirl-bags and store samples appropriately.
8.3.4.8 Throw away plastic spoon in trash and eject pipette tips in an autoclave bag and repeat with remaining samples.
8.3.5 To transfer samples to broth test tubes:
8.3.5.1 Label a sterilized test tube with sample identification and test type.
8 3 5 2 After preparing the work space as outlined in section 8 2, sanitize hands and don gloves
8 3 5 3 Open the sample bag
