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Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique
8.2 Preparation:
8.2.1 When a sample is delivered to the Microbiology Department, the sample is to be initially inspected by the Microbiologist setting the sample.
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8.2.2 If the sample is deemed acceptable for testing, the Microbiologist is to ensure the work space is cleaned and sanitized.
8.2.3 All solutions, media and broths to be used are to be sterilized in the autoclave via LP4010 Autoclave Operation, Cleaning and Maintenance. This is to be documented via LF3004 Autoclave logbook.
8.2.4 All test tubes, petri dishes, plastic loops, plastic spoons, pipettes, pipette tips and materials used in setting the sample are to be either pre-sterilized or sterilized in the autoclave
8 2 5 The Microbiologist performing the test is to ensure his or her hands are sanitized with soap and water or hand sanitizer prior to donning gloves
8 2 6 The Microbiologist is required to use gloves prior to handling the sample He or she is to change gloves whenever changing test methods or if the gloves become dirty or damaged
8 2 7 Using alcohol based hand sanitizer on the gloves can also be implemented, but should not be used in place of replacing damaged or dirty gloves
8 3 Sample Setting:
8 3 1 Attempt to work as efficiently as possible to reduce the sample’s exposure to the air
8 3 2 Solid Form or Powdered Samples:
8 3 2 1 Prepare a whirl-bag for each sample by labeling with sample identification and test type.
8.3.2.2 After preparing the work space as outlined in section 8.2, sanitize hands and don gloves.
8.3.2.3 Open the pre-labeled sample whirl-bag.
8.3.2.4 Place a plastic basket on the scale, then place the pre-labeled whirl-bag in the plastic bag and tare the scale.
8.3.2.5 Next, open the sample bag or sample container.
8.3.2.6 Open a sterile plastic spoon and scoop the appropriate amount of sample into the whirl-bag on the scale as indicated by the test method.
8.3.2.6.1 Never place the spoon on the counter.
8 3 2 7 After scaling, record the weights on the appropriate Laboratory Forms (LFs), close whirl bag and sample container
8 3 2 8 Throw away plastic spoon in trash and repeat with remaining samples
