Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique

7.1.4.1.2 The temperatures of the incubators will be checked and monitored twice daily via LP2001 Temperature Monitoring.

7.1.4.1.3 Once per Quarter, all incubators will be cleaned with the current microbiological cleaning solution as well as Sporicidin (M167).

7.1.4.2 Pipettes:

7.1.4.2.1 Pipettes will be used according to LP4004 Pipette Use and Maintenance Plan. Only trained personnel are authorized to use pipettes.

7.1.4.2.2 Pipettes that are visibly damaged are not to be used and are to be given to the Quality Department for service.

7.1.4.2.3 If pipettes are dirty or suspected to be contaminated via liquid entering the shaft of the pipette, do not continue to use Alert the Quality Department for service and/or autoclaving

7 1 4 2 4 Some pipettes are able to be autoclaved, the manual should be consulted prior to autoclaving and only trained and authorized personnel are to operate the autoclave

7 1 4 2 5 A new pipette tip is required to be used when switching solutions, this is to ensure cross contamination does not occur All pipette tips are to be bought presterilized or autoclaved prior to use in the Microbiology Laboratory

7 1 5 Work Surfaces:

7 1 5 1 Work surfaces are to be cleaned and disinfected every day prior to beginning testing They will be cleaned with the current cleaning chemical

7 1 5 2 Work surfaces will be sterilized with a sporicidin solution once per week to prevent spore growth

7.1.5.3 Work surfaces are to be cleaned whenever there is a sample spill, positive control spill, or if they appear especially dirty.

7.1.5.4 Storage of materials, media, equipment and samples will be done in a clean fashion.

7.1.5.5 Storage shelves and areas will be cleaned quarterly with the current chemical as well as with the sporicidin solution. They will then be rinsed with deionized water and wiped dry.

8.0 Procedure

8.1 Sample Receiving:

8.1.1 When new samples are received, they are not to be opened or tampered with in any fashion After being logged in according to LP1004 Test Sample Receiving and Processing, samples are to be delivered directly to their proper destination without delay