Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique

7.1.1.2 Sterile supplies are only to be used one time, then discarded. Plastic loops and needles, plastic spoons, pipette tips and Whirl-Bags are to only be used for one material and not transferred from sample to sample.

7.1.1.3 Supplies may be compromised during shipping. Upon receipt, supplies are to be inspected to ensure the integrity of the sterilization was maintained.

7.1.1.4 If supplies look damaged or compromised in any way, they are not to be used. The Microbiology Lead will be notified and the compromised supplies will be removed and replaced.

7 1 2 Media and Reagents:

7 1 2 1 Stock chemicals and reagents will be purchased pre-sterilized Upon receipt, Microbiologists are to inspect the stock chemicals and determine if they were compromised or contaminated in any way Suspicious looking chemicals or reagents will not be used and the Microbiology Lead will dispose of the chemical or reagent

7 1 2 2 Prepared media, broth and solutions will be autoclaved according their corresponding Laboratory Solutions (LSs) The autoclave will be cleaned, checked and operated via LP4010 Autoclave Operation, Cleaning and Maintenance

7.1 3 Environmental and Airborne Particles:

7 1 3 1 Airborne particulates can be a significant source of contamination The microbiology lab will be equipped with MERV (Minimum Efficiency Reporting Value) 8 Standard Pleated Air Filters on all vents to prevent the entry of any potentially contaminated material Air filters will be maintained and serviced by an outside agency quarterly to ensure they are in working order. They will be replaced every quarter regardless of their condition. This will be documented via LF4001 Equipment Maintenance Log.

7.1.3.2 Insects can be a source of contamination. There will be a pest control system in place throughout the lab. The system will be maintained once per month by an outside agency. Findings will be issued by the outside agency’s report.

7.1.3.3 One source of environmental contamination is other people visiting or working in the laboratory. The microbiology laboratory is to be segregated from other testing areas and access to the microbiology portion will be limited. Non-microbiology personnel are not to enter the microbiology laboratory without authorization from a microbiologists. Visitors and non-microbiology personnel are to adhere to all sanitation procedures while being in the microbiology laboratory This includes but is not limited to: eye protection, closed-toe shoes, long pants, long hair is secured and hand sanitation

7.1 4 Equipment

7 1 4 1 Incubators:

7 1 4 1 1 Incubators will be monitored for cleanliness and temperature via LP4011 Incubator Use, Cleaning and Maintenance