Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique

6.2 Chemical:

6.2.1 All work surfaces, counters and preparation areas will be cleaned daily prior to use. This is to ensure there is no cross contamination from surfaces to sample.

6.2.2 The chemical used to clean surfaces will be one of the following: 70% Isopropyl Alcohol Cleaning Solution (M147), an all-purpose cleaner, or Sporicidin Solution (M167)

6.2.3 Any changes to the cleaning chemical used is to be documented via LF2017 Deviation Record Form.

6.2.4 Once per week, the work surfaces, counters, and sample storage areas will be cleaned with a glutaraldehyde based sporical cleaning solution (M167). This is to prevent any spores from forming on work surfaces and contaminating test samples or materials.

6 3 Mechanical:

6 3 1 Items that cannot be disinfected or sterilized via chemical agents, will be cleaned via autoclaving

6 3 2 Prepared media, broths and solutions will be autoclaved according to their corresponding LSs This will be documented via LF3004 Autoclave Logbook

6 3 3 Operation of the autoclave is to be done by authorized and trained personnel only, the autoclave is to be operated via LP4010 Autoclave Operation, Cleaning and Maintenance

6 3 4 Autoclaves will be monitored for effectiveness via a few controls Autoclave cycles will be documented on LF3004 Autoclave Logbook This will monitor sample type, time and temperature of the autoclave The temperature of the cycle will be monitored internally to ensure the proper temperature for the cycle is reached

6.3.5 The samples being autoclaved will be marked with heat activated indicator tape also to ensure the samples reached the proper temperature.

6.3.6 Each cycle the autoclave is run, a biological indicator will be added to the cycle. This ensures the autoclave reaches the proper temperature as well.

6.3.7 All of these systems will be documented via LF3004. The autoclave is to be cleaned daily and maintained via LP4010 Autoclave Operation, Cleaning and Maintenance.

7.0 Sources of Biological Contamination

7.1 Even with proper aseptic technique, samples can still become contaminated and therefore compromised via various sources of contamination. The following is a list of most likely sources of contamination

7 1 1 Supplies:

7 1 1 1 The supplies used in the microbiology lab are sterile, one time use supplies These include sterile plastic loops, sterile plastic needles, sterile plastic spoons, sterile pre-filled pipette racks and sterile plastic Whirl-Bags