Chemical Hygiene Plan and Safety Manual - LP3013.4 Aseptic Technique

4.7 Bloodborne Pathogen: Any disease that can be spread through contamination via blood or any of its constituents including blood and blood plasma.

4.8 Antiseptic: An agent that inhibits or destroys microorganisms on living tissue. This includes but is not limited to: skin, oral cavities and open wounds.

4.9 Sporicidal Agent: An agent that destroys bacterial and fungal spores when used in sufficient concentration for a specified contact time. It is meant to kill all vegetative microorganisms.

4 10 Sterilant: An agent that destroys all forms of microbial life including fungi, viruses and all forms of bacteria and their spores

4 11 Disinfectant: A chemical or physical agent used to destroy or remove infectious fungi, viruses and bacterial This does not ensure the spores from these infecting agents will be destroyed Disinfectants only target pathogenic microorganisms

4 11 1 Chemical Disinfectant: A chemical agent used on inanimate surfaces and objects to destroy infectious materials

4 11 2 Physical Disinfectant: The use of primarily heat as an agent to destroy infectious materials on objects

5 0 Responsibility

5.1 Microbiology Personnel: the microbiology personnel are responsible for understanding the principles and following the protocols of aseptic technique. Microbiology personnel are not altering these procedures in any way unless instructed to do so by the Micro Lead.

5.2 Microbiology Lead: The Lead is responsible for understanding aseptic technique and ensuring the protocols within this procedure are being implemented correctly. It is the responsibility of the Lead to ensure the Microbiology Personnel are effectively trained and understand the importance of aseptic technique. Any modifications to testing protocols that may affect the aseptic technique procedure are to be documented and are to be done only with approval by the Lead.

5 3 Other Laboratory Personnel: Non-Microbiology personnel are responsible for knowing the importance of aseptic technique They are to respect the rules of the laboratory and are not to enter the Microbiology Department without purpose They are required to follow all hygiene and safety protocols as outlined in LP3001 – LP3008 Chemical Hygiene Plan and Safety Manual

6.0 Types of Sterilization

6 1 There are two types of sterilization implemented in the Microbiology Laboratory They are chemical and mechanical and will be outlined below