MiMode™ PuraBead® 96-well plates
Product code: FG00619, FG00620, FG00621
INTRODUCTION
The MiMode™ PuraBead® 96-well plate kit is a ready-to-use method development tool designed for high-throughput screening of mixed-mode chromatography conditions. Each well is pre-packed with 20 µL of Astrea Bioseparations Ltd and its affiliates’ (“Astrea Bioseparations”) mixed-mode chromatography resin.
Flexible operation
MiMode™ PuraBead® plates are compatible with both manual workflows (using multi-channel pipettes) and automated robotic systems. Their format supports high-throughput process development (HTPD) by enabling simultaneous screening of a wide range of chromatographic conditions.
Available formats
Plates are available in single-resin or multi-resin formats, offering flexibility for both targeted optimization and broader condition scouting. See the ‘Ordering information’ section for more details.
Accelerated development
By allowing parallel analysis of multiple variables, these plates help quickly identify optimal process conditions across a broad experimental space. Once target conditions are identified, scale-up and process optimization are easily performed using compatible pre-packed columns such as 1 mL & 5 mL HT pre-packed columns, Evolve® R 5 mL or 50 mL columns, or through bulk resin on ÄKTA™ systems.
Kit components
Each kit includes:
• One MiMode™ PuraBead® 96-well plate
• A wash plate
• A collection plate
These components provide everything needed to carry out parallel experiments efficiently.
Important product information
• For resin description, plate configuration, and additional information (data), please see the attached product flyer.
• If the purification is performed using centrifugation, the spin plate must be balanced throughout the procedure with a duplicate plate or a balance plate filled with an appropriate volume of water.
• Empirically determine the optimal buffer (pH and salt concentration) for purifying and eluting the protein of interest based on the pI and hydrophobicity of the protein.
When using MiMode™ PuraBead® 96-well plates with an automated system, follow instructions provided by the equipment manufacturer with regards to plate set-up and method programming.
Additional materials required
• Variable-speed centrifuge with rotor and carriers capable of handling stacked plates (4.4 cm height) at 500 × g, a vacuum manifold or positive pressure head system. Suggested flow rates are 4–8 inches Hg (2–4 psi), which is equivalent to 1–2 drops/second.
• Multi-channel pipettor and tips.
