PuraBead
®
PuraBead® is manufactured by Astrea Non-toxic raw materials:
Security of supply • Reliably manufactured for over 20 years
Water Agarose Buffer
Consistent quality
No oil or solvent required
• Manufactured to ISO9001 accredited standard • Supported with a comprehensive Regulatory Support File (include leachables and extractables)
PuraBead® is produced using a novel atomization technology
Precisely controlled flow rate and temperature
Beader
Agarose Preparation
Spinning disc technology creates mono-dispersed agarose droplets of predetermined size
Heat Exchanger Uniformly sized beads = consistently superior flow properties
Agarose droplets
1000L batch sizes available if required • Continuous process means batch sizes can be varied according to need
Reduced environmental impact for greener manufacturing • Unlike competitor resins, PuraBead® does not require the use of organic solvents in the beading process Water recirculation tank
Uniform bead size and flow properties
PuraBead®
PuraBead® size is significantly more uniform than a comparable market leading product PuraBead®
Market leading product
1000
1000
800
800
Pressure vs Flow
1000
600 400 200
Linear Flow Rate (cm/hour)
Frequency
Frequency
800
600 400 200
0 100
150
600
400 PuraBead® 200
0 50
50
Diameter (ESD)
Non-uniform packed array due to broad size distribution
Cross Linking
Superior size distribution and flow properties
The PuraBead® manufacturing process results in uniform beads with near mono-disperse particle size distribution and excellent porosity and flow properties.
Uniform close-packed array
Un-crosslinked beads
Bead Collection
100
Market leading product
150
Diameter (ESD) 0
Porosity (Kav)
0.40
Porosity (Kav)
0.35
Flow (cm/hr at 1 Bar)
897
Flow (cm/hr at 1 Bar)
525
Mean Diameter (µm)
92.9
Mean Diameter (µm)
96.36
Distribution (% beads in range 76-141µm)
99%
Distribution (% beads in range 76-141µm)
66%
Pressure flow data was generated using a bed height of 15cm and an internal diameter of 32mm. Porosity data was generated using Thyroglobulin at ~670kDa.
0.0
0.2
0.4
0.6
0.8
1.0
Pressure (Bar) Pressure flow curves were generated using a bed height of 15cm and an internal diameter of 32mm.
1.2