2020 AALAS Virtual National Meeting Program

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CONTENTS

AAL AS 71 st N AT I ON AL MEET I N G

PROGRAM COMMITTEE Chair: Chris A Manuel

WELCOME

Vice Chair: Rachel R Rubino

Program Chair’s Message President’s Message Keynote Speaker

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EXHIBITORS & SPONSORS Exhibitors & Sponsors

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EDUCATION TRACKS Technical Trade Presentations Animal Welfare, Regulatory Compliance, and Public Education Biomedical Research, Medicine, and Methodology Facility Design, Management, and Operation Overcoming Challenges Platform Sessions Posters

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MEETING NEWS & EVENTS Branch & District Information

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Seminars Chair: Sarah Hansen Vice Chair: Kristin D Evans Special Topic Lectures Chair: Jennifer L Asher Vice Chair: Karen L Lencioni Panel Discussions Chair: Temeri Wilder-Kofie Vice Chair: Jennifer S Wood Workshops Chair: Sean C Adams Vice Chair: Natasha J Melfi Facilitators Chair: Glicerio A Ignacio Vice Chair: Heather E Marshall Local Arrangements Chair: Julie V Vineyard BOT Liaison, President, ex oficio Tracy Parker Abstract Review Subcommittee Chair: Kari L Koszdin Platform Session: Tanya L Herzog Poster Session: Maggie Behnke, James B Finlay, Lauren Habenicht, Rachel Larsen, Sharoll L’Italien, Adam Murphy, Karuna Patel, Karen Strait, Wanda West

EXHIBITOR ADVISORY COUNCIL Chair: Joseph P Guider Vice Chair: Paulina Michaud Warren Ball, Eugene Marino, Todd Poley, Fredrick Rock, Carrie Schultz, Perry Spires, Chris Manuel, John Park Past President: Morag Mackay BOT Liaison: Teresa Woodger

EXECUTIVE DIRECTOR Ann Turner

ADVERTISER INDEX Allentown 41 Bio-Serv 49 Clear H2O 5 E-Z Systems/Euthanex 3 Fidelis Pharmaceuticals 39 Hilltop Lab Animals Inc 37

Kent Scientific Corp 33 Lab Products Inc 52, IBC Marshall BioResources 1 PMI LabDiet IFC Priority One Services Inc back cover SteraMist 36

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AALAS WELCOMES YOU! On behalf of the National Meeting Program Committee (NMPC), I would like to extend some virtual hospitality and welcome everyone attending to the 71st AALAS Virtual National Meeting! Serendipitously, the spotlight forum of this year’s meeting is “Overcoming Challenges,” which is timely considering the world around us. We have all heard that this is extraordinary times because it is extraordinary times. Over that last 7 months, we have all faced unique challenges and flexed our creativity while managing limited resources. This year’s program includes many fascinating examples of how our colleagues have overcome a variety of challenges, in addition to those that are obvious. There are also many other exciting, educational, and relevant submissions, which are sure to give all AALAS members numerous topics of interest to choose from throughout the meeting.

I want to thank the NMPC, AALAS staff, and the Local Arrangements Committee for all their time and effort in getting this meeting off the ground and onto the track. This amazing group of people put in a colossal amount of effort, especially this year, to create the firstever AALAS National Meeting virtual meeting platform. We can’t thank them all enough. Finally, thank you to all who submitted topic ideas and abstracts despite the challenges you faced. Your drive to share your knowledge and exciting research are what makes each meeting a unique and worthwhile educational experience for all AALAS members.

Chris Manuel 2020 Program Committee Chair

WONDERFUL OPPORTUNITIES IN THE VIRTUAL MEETING! I want to welcome you to the 71st AALAS Virtual National Meeting! In what has undoubtedly been a tumultuous year, I am still excited that the NMPC has been able to assemble a top rate program full of educational opportunities for our members. No matter your background, there are plenty of topics available for you.

The theme of this meeting is “Overcoming Challenges,” which I chose without any foresight into what 2020 would bring for us. Having said that, the program has a fair amount of COVID topics, but that is just the beginning. We all know that we overcome challenges every single day in our facilities, our research, and our lives. Join us if you want to find out more! I hope to “see” you in person soon.

Tracy Parker 2020 AALAS President

5 YO UR PAR TNER IN

BREEDING

4 Nutritional support for breeders

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Breeding Support

4 Improve breeding performance 4 Formulated to increase litter size and enhance breeder health 4 Highly palatable and easy to use

NURSING 4 Nutritional support for lactating females 4 Decreases cannibalism 4 Improves pup weight and health 4 Supports breeder recovery

WEANLINGS

4 Nutritional support for weanlings 4 Replaces mash diets 4 Improves weanlings and runts survival 4 Helps pups acclimate to their new cage

HydroGel

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DietGel

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MediGel

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MediDrop

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LabGel

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Bites

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VetGel

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AquaGel

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GOLD

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We look forward to seeing you at AALAS 2020 Virtual National Meeting To request a sample and learn more about our full line of products, go to clearh2o.com or call today at 1-888-493-7645.

OPENING GENERAL SESSION/ GENERAL MEMBERSHIP MEETING Sunday, October 25, 2020 5:00-6:30 PM EST • • • • • •

Welcome from AALAS President, Tracy Parker AALAS Awards presentations Recognition of guests and Board of Trustees members Gavel ceremony introducing the new president Introduction of new trustees Incoming president, Doug Taylor’s address

BRANT MENSWAR, KEYNOTE SPEAKER With his authentic rock star swagger, Brant Menswar challenges us to become values-based leaders, empowers us to choose our purpose and inspires us to change what’s possible with his incredible story of hope, creativity and determination.

Brant Menswar is a critically acclaimed author, award-winning musician and the CEO and founder of Rock Star Impact, a boutique agency that teaches people and organizations how to cultivate values-based leadership and activate organizational values to help connect the head and heart. His unique and compelling approach of using music to build bridges across generational, gender and competency-based gaps has changed what’s possible for industry-leading organizations like Netflix, Verizon, St. Jude Children's Research Hospital, Microsoft, Hilton and dozens more.

Passionate, engaging, and transformational, Brant encourages audiences to move forward with deliberate intention. His interactive and entertaining techniques of defining what matters most compels audiences to dive deeper into their lives, identify their core values and give them the airplay needed to become life-changing anthems that change what’s possible.

Unbreakable Leadership: Engaging the Power of Purpose

The world’s greatest illusionist, Harry Houdini, once said, “What our eyes see and our ears hear, our mind believes.” Strong leaders learn to control those everyday illusions by filtering outside influences and acting with deliberate intention. In this high-energy, interactive presentation, Brant Menswar shows you how to do that immediately and effectively daily. He illustrates that – despite what we’ve been told – we all need to start with our “What” in order for our “Why” to be accurate. Discover how to identify your non-negotiables to amplify and align with your organizational values. With his Rock 'N' Roll infused session, Brant shows audiences how to forge unbreakable leaders and empower them to choose their purpose.

Photos courtesy of Keppler Speakers

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PROGRAM SPONSORS PRESIDENTIAL SPONSORS

GOLD SPONSORS

SILVER SPONSORS

EXHIBITORS & SPONSORS

Alfa Wassermann Diagnostic Technologies 4 Henderson Drive West Caldwell, NJ 07006 Phone: 973-852-0244 www.alfawassermannus.com Alfa Wassermann Clinical chemistry systems use liquid, ready-to-use reagents, providing an inexpensive alternative to dry chemistry. To help maximize your research success, stay competitive, and meet deadlines, Alfa Wassermann offers a compact, cost-effective, easy-to-use, and innovative benchtop analyzer.

Allentown, LLC 165 Route 526 Allentown, NJ 08501 Phone: 609-259-7951 Email: info@allentowninc.com www.allentowninc.com Allentown is a leading global provider in Life Science solutions. For 52 years we’ve been proud to play our part in heroic biomedical research that saves lives. To this day, care is at the heart of everything we do. Care for each laboratory housing product that rolls off our factory floor; care for our customers and the patients who ultimately benefit from the work; and care for the welfare of the animals whose health and comfort are critical to the success of the research.

Alpha Genesis Inc. 95 Castle Hall Road Yemassee, SC 29945 Phone: 843-597-0000 www.alphagenesisinc.com Alpha Genesis Inc. (AGI) is a direct provider of nonhuman primates to the biomedical community. AGI is dedicated to high-quality management and oversight of its primate colonies and is one of the few providers to offer both U.S-bred and imported nonhuman primate research models, conditioned to meet your specific research needs. Proven breeding programs and AAALAC accredited facilities translate into care and preparation of nonhuman primates that far exceeds industry standards.

Alternative Design Manufacturing & Supply Inc Gold Sponsor 3055 Cheri Whitlock Dr PO Box 6330 Siloam Springs, AR 72761 Phone: 479-524-4343 Fax: 479-524-4125 www.alternativedesign.com Alternative Design Manufacturing & Supply, Inc. offers an extensive line of laboratory animal housing for species large and small.

We have a full range of material handling and processing equipment. Custom metal fabrication is welcome. For 30 years we have built a reputation for quality products and custom innovative solutions to meet the ever-changing industry. When innovation, quality, and service matter, contact the original Alternative, backed by an industry-leading warranty program!

ALZET® Osmotic Pumps/DURECT Corp 10260 Bubb Road Cupertino, CA 95014 Phone: 408-367-4036 Email: alzet@durect.com www.alzet.com ALZET® pumps are small, implantable devices designed to deliver agents at precise rates to lab animals as small as mice. These pumps are a simple, cost-effective, and superior alternative to repeated injections and external pumps. They deliver compounds for 1-42 days without further animal handling; and no external connections, batteries, or electronics that can fail. Made in the USA, ALZET pumps have been used more than 40 years in 19,500+ publications that demonstrate their reliability.

American College of Lab Animal Medicine 96 Chester St Chester, NH 03036 Phone: 603-339-7039 www.aclam.org ACLAM is recognized by the AVMA to certify specialists in Laboratory Animal Medicine as recognized specialists in the field of Veterinary Medicine.

American Protective Products 8 Custom Drive Old Saybrook, CT 06475 Phone: 203-927-9264 At American Protective Products, we are specialists in providing high-volume disposable apparel to health, safety, and research facilities throughout the USA. Combined we have close to 50 years’ experience as distributors of personal protective equipment. We offer the most up-to-date products, along with competitive pricing and exceptional personalized service.

American Society of Laboratory Animal Practitioners (ASLAP) 9190 Crestwyn Hills Dr Memphis, TN 38125 Phone: 901-333-0498 www.aslap.org The American Society of Laboratory Animal Practitioners is a professional member association open to ALL veterinarians, veterinary residents, and veterinary students who are engaged in or interested in promoting and supporting laboratory animal practice.

ASLAP is the only organization that directly represents laboratory animal practitioners within the American Veterinary Medical Association.

Americans for Medical Progress 444 N. Capitol Street NW Washington, DC 20001 Phone: 202-624-8812 www.amprogress.org Americans for Medical Progress is dedicated to protecting your investment in biomedical research. We focus on public outreach that builds understanding and appreciation for necessary and humane animal research. We also provide vital news, information, and analysis to biomedical research stakeholders to ensure they have the resources they need to deflect campaigns that threaten the future of medical progress. We are a national 501(c)3 nonprofit supported by medical schools, research institutions, and people like you!

Ancare Corp 2647 Grand Ave PO Box 814 Bellmore, NY 11710 Phone: 516-781-0755 Email: sales@ancare.com www.ancare.com Ancare offers a wide range of products for animal care... It’s really so much stuff and no one is going to read about it here! Instead, just look around, and reach out if you can’t find what you are looking for or need more information.

Animal Care Systems, Inc. 7086 S Revere Parkway Centennial, CO 80112 Phone: 720-283-0177 Email: info@animalcaresystems.com www.animalcaresystems.com Animal Care Systems, in partnership with ARES Distribution, specializes in providing the highest quality equipment for the vivarium and the laboratory for all your research applications. Animal Care Systems was founded in 1997 by a PhD, DVM Neuroscientist and our consultative representatives strive to continually innovate and search for new product lines to provide diverse solution-oriented equipment and services, while remaining focused on animal and personnel welfare.

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AAALAC International 5205 Chairman’s Court, Suite 300 Frederick, MD 21703 Phone: 301-696-9626 Fax: 301-696-9627 www.aaalac.org AAALAC International promotes the humane treatment of animals in science, research and education through voluntary assessment, accreditation, and education programs. More than 1,000 institutions in 49 countries have earned AAALAC accreditation, demonstrating their commitment to responsible animal care and use.

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EXHIBITORS & SPONSORS Animal Care Training Services (ACTS) 340 Cypress Circle King Of Prussia, PA 19406 Phone: 484-574-7455 Email: info@actstraining.com www.actstraining.com We are committed to providing exceptional training services and programs in all areas of laboratory animal care and use. Our staff represents the best in the industry and is led by a board-certified laboratory animal veterinarian.

Animal Facility Software 90 East Halsey Rd Parsippany, NJ 07054 Phone: 646-326-7942 Email: martin@animalroomlog.com www.animalroomlog.com Animal Identification & Marking Systems, Inc. 99 Park Drive Hornell, NY 14843 Phone: 607-324-6752 www.animalid.com AIMS laboratory animal tattoo identification systems have been used for over 40 years to identify research animals of any age safely and permanently. Specialized tattoo kits are available for mice & rats, neonates, and large lab animals. AIMS tattooing procedures are used extensively to rapidly genotype animals before weaning. Each tattoo kit comes with comprehensive equipment and self-training instructions. On-site tattoo certification training courses are also available.

Animal Resource Management 16466 Bernardo Center Dr Ste 283 San Diego, CA 92128-2533 Phone: 619-596-9404 www.armsd.com Manage your animal care facility with ARM, an easy-to-use, multi-user computer program that tracks protocols, animal ordering, and animal inventory; bills for purchases, services, and per diems; tracks surgeries, procedures, treatments, and patient history. ARM also offers web-based animal ordering, email reports to end-users, and cost accounting analysis. ARM is NIH, 21 CFR part 11, and GLP compliant. Visit us at booth #1321.

Arcoplast Inc 1873 Williamstown Dr St Peters, Missouri 63376 United States Phone: 636-978-7781 Fax: 636-978-7782 ghislain@arcoplast.com http://www.arcoplast.com

Art's Way Scientific 203 Oak St Monona, IA 52159 Phone: 563-539-2336 www.buildingsforscience.com Art’s Way Scientific is a leading producer of technical turnkey research, vivarium, and diagnostic laboratories. Art’s-Way designs, develops, manufactures, and installs a complete custom engineered building for laboratory animal research, biocontainment, public health, food safety, and general laboratory space requirements. www.buildingsforscience.com Asher Technologies LLC 13551 Highland Mews Pl Herndon, VA 20171 Phone: 571-309-9796 www.ashertechnologies.us Animal Research Data Management System (ARDXX) is the next generation tool to manage animal research data. It is a onestop solution that captures and stores animal research information electronically. This user friendly and efficient tool greatly reduces the time spent in logging and categorizing research data.

Association of Primate Veterinarians 9190 Crestwyn Hills Dr Memphis, TN 38125 Phone: 901-754-8620 www.primatevets.org The Association of Primate Veterinarians (APV) is an international organization of approximately 500 veterinarians concerned with the health, care, and welfare of nonhuman primates. We provide mechanisms to disseminate information and speak collectively as primate veterinarians on matters regarding nonhuman primates and to promote fellowship among primate veterinarians.

Avidity Science 819 Bakke Avenue Waterford, WI 53185 Phone: 262-534-5181 Fax: 262-534-5184 www.avidityscience.com Avidity Science serves the biomedical, scientific research and healthcare industries on a global scale. We are the world-leading supplier in automated animal watering systems with the most reliable drinking valves on the market and more than 17 million valves in operation which deliver purified water and eliminate ergonomic injuries. Our water purification and treatment systems provide consistent water quality. Our Watchdog EX™ environmental monitoring software protects valuable research.

BASi 2701 Kent Ave West Lafayette, IN 47906 Phone: 765-418-4384 www.basinc.com BASi is a manufacturer of high quality, awake animal sampling products. The Culex® automated blood sampling systems enhance animal welfare and subsequent data quality by reducing stress for animals and humans involved. We are known for our support and creativity in making your studies possible. Come see why!

BetterBuilt 7601 MacDonald Road Delta, BC, V4G 1N3 Canada Phone: 604-952-0925 Fax: 604-952-0941 www.nsc-betterbuilt.com BetterBuilt manufactures cage washing equipment, decontamination chambers, laboratory glassware washers, and necropsy fixtures. We also provide Sure-Flo™ and Sure-Vac™ bedding handling systems as well as Activ™ Automation products. We help improve workplace ergonomics, reduce exposure to allergens and increase worker safety along with productivity. We invite you to review our complete product offering of unique solutions designed for use in Vivarium and Laboratory Research Facilities.

Bio Medic Data Systems, Inc. 1 Silas Road Seaford, DE 19973 Phone: 302-628-4100 Fax: 302-628-4110 www.bmds.com BMDS offers RFID readers and transponders as a solution for accurate animal identification. Our advanced technology includes user-programmed animal numbering and micro-identification for neonatal models.

Our injectable transponders can display ID and body temperature. Our reader systems display the animal IDs, as well as collect data from other devices, such as calipers and animal balances. Our products can interface with any data collection software program. bioBUBBLE Inc. 1411 E. Magnolia St. Fort Collins, CO 80524 Phone: 970-224-4262 www.biobubble.com BioBUBBLE provides creative solutions for environmental challenges in the research world. We custom design and manufacture an affordable line of HEPA-filtered modular cleanrooms and containment enclosures for anything from germ-free and gnotobiotic to BSL, 2, 3 and 4 applications. HEPA-filtered support equipment includes anterooms, air showers, bedding disposal units, transport carts, and air diffusion units. Facility design consultation services are available.

EXHIBITORS & SPONSORS

Brain & Software International 990 Biscayne Blvd Office 701 Miami, FL 33132-1556 Phone: 877-977-5775 www.poweredbyenos.com Enos is an adaptable software solution for managing all resources in lab animal facilities: vivarium-space, cages and cage cards, animal health records, equipment, drugs & consumables, personnel and more. Schedule and track activities such as breeding, colony management, animal care, equipment maintenance to ensure efficiency and quality. We work closely with clients to review processes, workflows, requirements, and objectives to deliver and support a configured and secure platform.

Braintree Scientific Inc. PO Box 850498 Braintree, MA 02185 Phone: 781-917-9526 Fax: 978-244-8917 www.braintreesci.com Braintree Scientific, Inc. - Over four decades of experience serving the needs of the Life Science Industry by finding, carrying and/or creating the products you need. We offer our exclusive product line - MicroRenathane® tubing, Deltaphase® Isothermal heating pads, Decapicones® - as well as an expansive list of research items focusing on mice and rats. As your needs expand so does our product line.

Britz & Co. 1302 9th Street Wheatland, WY 82201 Phone: 307-322-4040 Fax: 307-322-4141 Email: sales@britzco.com www.britzco.com Founded by a pioneer in Laboratory Animal Medicine, our Company has a unique historic perspective and a clear understanding of current animal care issues that affect the housing of captive animals in a research environment. With our knowledge base, we custom design, fabricate, install and service a comprehensive line of animal housing

systems, animal isolation cubicles, and care products for the biomedical research industry. Our products are imaginatively designed and fabricated in the U.S.A. BZ Sales & Support LLC 3042 Lizzie Melton road Chester, SC 29706 Phone: 803-493-4012 bzsalesandsupport.com BZ Sales & Support is introducing BioZoneGlobal's new innovative products, providing easy access for PCR testing. The SmartRack-II utilizes hi-temperature silicone seals, eliminating expensive welding and providing value for your dollar, while still maintaining hi-ACH/low-velocity animal environment. The new DigiFlow II" now provides in-room computing capability in addition to DigiFlow’s Environmental Monitoring and Control System.

CannonDesign 1560 Wilson Blvd. #200 Arlington, VA 22209 Phone: 703-915-7013 www.cannondesign.com CannonDesign is an integrated design firm that leverages the heart, passion, and intelligence of our more than 1,000 creative thinkers to develop solutions for some of the greatest challenges facing our clients and society. Whether designing for equity in education, accessible healthcare, inclusive communities, new scientific discoveries or the “next big idea” in business, we leverage our full suite of end-to-end services, we partner with organizations to help them improve human life.

Cayuse 121 SW Salmon Street Suite 900 Portland, OR 97204 Phone: 503-297-2108 www.cayuse.com Cayuse provides a connected research administration cloud platform with solutions that covers the entire research lifecycle, including pre-award, post-award, risk management, and resource management.

Charles River President Sponsor 281 Ballardvale Street Wilmington, MA 01887 Phone: 877-274-8371 www.criver.com We support today’s most critical research with the timely delivery of study-ready, preconditioned and genetically engineered animals. Our leading scientists and global network of AAALAC-accredited vivaria and breeding facilities provide clients with accessible, expert management of outsourced colonies, biology services for line creation, maintenance and preservation, advanced

diagnostics, and health monitoring to guarantee the long-term success of their work.

Charles River: More than a mouse.

ClearH2O Gold Sponsor 34 Danforth Street Portland, ME 04101 Phone: 207-245-6317 www.clearh2o.com ClearH2O is a life science company meeting the needs of industry researchers and breeders by providing products that optimize delivery of hydration, nutrients, and therapeutics to enrich animals’ lives. We apply proprietary gel technology to develop, manufacture, and market cost-effective solutions to address unmet animal husbandry needs, while saving time and labor.

Colonial Medical Supply Co., Inc. 12 Parmenter Rd. D-5 Londonderry, NH 03053 Phone: 603-404-2105 Fax: 603-404-2138 www.colmedsupply.com For over 40 years, Colonial Medical Supply has been dedicated to delivering the highest standard in medical equipment, personalized customer service and on-site anesthesia machine maintenance to the animal health community. With our constant attention to the available leading technology, we strive to have a positive impact on the care and productivity that is provided by the many professionals in this important field.

Covetrus 400 Metro Place North Dublin, OH 43017 Phone: 614-659-1874 www.northamerica.covetrus.com Visit the Covetrus Virtual Clinic to walk through all of our products, solutions and technology we offer to help you grow and empower your practice. Similar to a gift registry, you will have the opportunity to add products and solutions to your Wish List.

Each area you visit earns you an entry into our sweepstakes for a $250 credit towards your Wish List items. Visit at covetrusvirtualbooth.com CURIS System 1713 Kennedy Point, Suite 1001 Oviedo, FL 32765 Phone: 800-928-8708 www.curissystem.com CURIS® System provides the technology and versatility you need to achieve validated biological safety. We protect your research, assets, and personnel from loss due to potential contaminants. CURIS® products and services enable an easy, cost-effective means to complete decontamination using Pulse™ driven hydrogen peroxide Micron Mist™ for the most comprehensive and safe decontamination possible.

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Bio-Serv 3 Foster Lane Flemington, NJ 08822 Phone: 908-284-2155 Fax: 908-284-4753 www.bio-serv.com Bio-Serv has been serving the research community for 50 years, and our mission is to create and provide products that meet the unique challenges associated with working with research animals. Our experts in lab animal nutrition and animal welfare understand the requirements of caring for research animals and are dedicated to meeting the ever-changing needs of the research community. Having developed many unique enrichment devices and formulated thousands of diets, we are here to assist you.

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EXHIBITORS & SPONSORS Drexel University 245 North 15th Street, Philadelphia, PA, 19102 Philadelphia, PA 19102 Phone: 267-337-0450 www.drexelmed.edu/medicine/academics/ graduate-school/master-of-laboratory-animal-science/ Drexel University in Philadelphia, Pennsylvania is the official home of the renowned “MLAS” degree! The MLAS program has trained students in the science, care, and management of laboratory animals for more than 30 years. Our mission is to improve the lives of people and animals by educating the leaders of tomorrow. The program is available 100% online and can be completed in 3 years or less! Students interested in applying to veterinary medical school can minor in pre-veterinary studies.

Eastern Virginia Medical School 700 W Olney Road Norfolk, VA 23501 Phone: 757-446-5634 Email: hosseia@evms.edu www.evms.edu The Laboratory Animal Sciences Master Program (MLAS) at Eastern Virginia Medical School is an almost entirely distance education program to prepare laboratory animal professionals for the rapidly evolving science, technology, ethical, and regulatory issues. MLAS may also improve career advancement in veterinary care or research and potentially strengthens the veterinary medicine school applications.

Elm Hill Labs 7 Kidder Rd Chelmsford, MA 01824 Phone: 978-430-5939 www.elmhilllabs.com Elm Hill Labs has specialized in the production of guinea pigs exclusively for the research community for over 60 years. All Elm Hill Hartley guinea pigs and multicolored guinea pigs are virus-antibody-free. We take pride in being recognized throughout the industry for the excellent quality of our guinea pigs, for outstanding service, and for our genuine concern for each customer's particular needs.

Envigo 8520 Allison Pointe Blvd, Suite 400 Indianapolis, IN 46250 Phone: 317-749-8237 www.envigo.com Envigo provides the broadest range of research models to the pharmaceutical and biotechnology industries, government, academia, and other life science organizations.

As the largest organization that is solely dedicated to providing research models and related products and services, we are committed to helping researchers realize the

full potential of their critical R&D projects as we fulfill our mission to work together to build a healthier and safer world. Read more at envigo.com Esco Technologies, Inc. 903 Sheehy Drive Suite F Horsham, PA 19044 Phone: 215-441-9661 Fax: 484-698-7757 escolifesciences.us Esco is the world leader in advanced biosafety cabinets and animal containment workstations. Esco cabinets and workstations feature energy efficient DC-ECM blowers and ULPA filters with 10 times better filtration than same-age HEPA filters.

With a large performance envelope, anti-microbial coating, and durable steel plenum, Esco products are low noise and have Riboflavin verified cleanability. In addition, Esco offers ELISA-verified allergen containment and user-friendly controllers.

E-Z Systems/Euthanex Corp.

PO Box 3544 Palmer, PA 18043 Phone: 610-882-3800 Email: info@euthanex.com www.ezsystemsinc.com E-Z Systems features the Euthanex SMARTBOX Automated CO2 systems, E-Z Anesthesia, E-Z Trak, and E-Z Covers products to meet your animal research needs. We offer humane euthanasia solutions, user-friendly anesthesia equipment, comprehensive animal management software, custom-made cart covers, and more. Visit us to learn how we can help. The choice is E-Z! FEFA, LLC 2106 Beckett's Ridge Drive Hillsborough, NC 27278 Phone: 919-316-0188 www.fefalac.com FEFA, LLC specializes in providing exceptional animal care in a wide range research settings. From cage processing, to husbandry, to technical support, to IACUC administration, to veterinary care, our team of contract lab animal professionals collaborate with our clients to achieve an exemplary standard of animal care across all programmatic functions. With a corporate support team comprised of industry experts with decades experience, FEFA provides an unrivaled caliber of service.

FeSAHANCCCAL Tepeaca Granjas Del Sur Puebla Puebla 72470 Mexico Phone: 522223933649 Email: fesahancccal@gmail.com www.fesahancccal.com FESAHANCCCAL supports the development of laboratory animal science societies and associations in Central America and the Caribbean. Other endeavors include

promoting the care and use of laboratory animals in accordance with legal and ethical principles; promoting events where specialists in LAS in Central America and the Caribbean can share information; disseminating scientific research in Central America and the Caribbean; and the teaching, training, and certification of persons in LAS in Central America and the Caribbean.

FiberCore, LLC 15325 Saranac Road Cleveland, OH 44110 Phone: 216-249-2100 Fax: 216-249-2112 www.fibercorellc.com Manufacturers of Envirodri, Forage Boxes refilled with Envirodri and other enrichment products. Everything is made in USA. Fidelis Pharmaceuticals 675 Highway One, Suite B113 North Brunswick, NJ 08902 Phone: 833-384-4729 ethiqaxr.com Fidelis Pharmaceuticals is the proud maker of NEW Ethiqa XR™, a pharmaceutical-grade, extended-release buprenorphine that’s FDA-affirmed to control post-procedural pain for laboratory mice and rats up to 72 hours with one injection. Fidelis Pharmaceuticals is deeply committed to improving animal welfare with innovative solutions for laboratory animals. The Fidelis leadership team supports the foremost veterinary, laboratory research, and human healthcare industry associations and initiatives.

Fine Science Tools 4000 East 3rd Ave, Suite 100 Foster City, CA 94404 Phone: 800-521-2109 www.finescience.com Fine Science Tools™ offers over 1000 high-quality European surgical instruments for research scientists and veterinarians worldwide!

Foundation for Biomedical Research 1100 Vermont Ave. NW, Suite 1100 Washington, DC 20005 Phone: 202-457-0654 www.fbresearch.org Together with the National Association for Biomedical Research, we seek to create the resources to benefit the public, the government, and research professionals alike. There can be no science in a vacuum.

Everything we do is part of a conversation.

We hope you’ll join it.

Fuel3D - BioVolume Fuel3D, Schrodinger Building, Oxford Science Park, Heatley Road Oxford, Oxfordshire OX4 4GE United Kingdom Phone: +44 7725749338 www.biovolume.com

EXHIBITORS & SPONSORS

Galilei Software GmbH Prof.-Max-Lange-Platz 6 Bad Toelz 83646 Germany Phone: +49 (0) 8024 470 470 Email: contact@galileisoftware.com www.galileisoftware.com The Galilei CageTalkers® - electronic cage cards - replace printed cage cards and offer following advantages: • Paperless Animal Room • Permanently updated census • Web service to integrate any Animal Management System & MSExcel • For every type of cage • Automatic cage identification and tracking • Individual layout of cage cards • Signaling alerts, statuses and tasks by LEDs further supported by colored buttons • Displaying the individual data of all user groups on 1 dynamic electronic cage card.

GenoTyping Center of America 415 Water Street Ellsworth, ME 04605 Phone: 207-329-0189 www.gtcasolutions.com The GenoTyping Center of America provides fast, accurate, and comprehensive genetic testing services. Our proprietary DNA isolation methods, independent assay verification, and PCR melt curve analysis deliver results in 2-3 business days. We freeup valuable resources and reduce costs so you can stay focused on your research.

Getinge Silver Sponsor Lindholmspiren 7 Gothenburg SE-417 56 Sweden Phone: +46 (0)10 335 0000 www.getinge.com/us/ Getinge Life Science offers equipment, technical expertise and consultative services.

Together, these solutions meet both common and highly specialized process needs for contamination prevention in biopharmaceutical production, biomedical research, heat sensitive goods manufacturing, and laboratory applications.

Girton Manufacturing Co., Inc. 160 West Main St. Millville, PA 17846 Phone: 570-458-5521 Email: Lab1@girton.com www.girton.com

Girton manufactures a full line of environmentally friendly washers for cages, racks, bottles, and miscellaneous items used in the care of laboratory animals. Bedding dispensers, bottle fillers, and a variety of washing racks complement our washer lineup. Girton engineers work closely with your designers when planning new or upgraded facilities.

Gruenberg-TPS 2821 Old Route 15 New Columbia, PA 17856 Phone: 570-538-7200 Fax: 570-538-7380 www.gruenberg.com Gruenberg, a division of Thermal Product Solutions, is a leader in dry heat sterilization technology with an advanced line of sterilizers that eliminate harmful pathogens on animal habitat cages. Gruenberg Steri-Dry™ dry heat sterilizers are a sustainable alternative to steam autoclaves for a fraction of the price. The sterilizers are built to ensure a smooth and quiet operation, beneficial when working with noise-sensitive animals.

Hilltop Lab Animals, Inc. 131 Hilltop Drive Scottdale, PA 15683 Phone: 724-887-8480 www.hilltoplabs.com Hilltop Lab Animals, Inc. produces research animals including rats, mice and guinea pigs. Hilltop also provides contract housing including aged animals, precisely time-mated animals, tissues, blood products, and wide variety surgical procedures including catheter (vascular, bile duct, intra-gastric, urinary bladder) implants. For more information call customer service at 724-8878480.

Hydropac 742 Sussex Ave. Seaford, DE 19973 Phone: 302-628-4300 www.labproductsinc.com Hydropac® offers an alternative watering solution for your research that remains Germ-Free while in use with rodents or in storage, without the hassle of bottle or the high cost of automatic systems.

IDEXX BioAnalytics Gold Sponsor One IDEXX Drive Westbrook, ME 04092 Phone: 207-556-4929 www.idexxbioanalytics.com IDEXX BioAnalytics research diagnostics and services offer quality that exceeds expectations, innovation that expands possibilities, and world-class expertise that instills confidence, all in one powerful partnership: IDEXX BioAnalytics and you. We have been serving the bioresearch community for 50 years with animal health

monitoring, pathology services, biological materials testing, and veterinary clinical trials services. iMedRIS Data Corporation Silver Sponsor 446 Missouri Ct Redlands, CA 92373 Phone: 909-798-9100 www.imedris.com iMedRIS provides state-of-the-art electronic research administration software in the form of innovative research compliance and administration management system called iRISTM (integrated research information system). iRISTM is the most versatile and comprehensive research software suite available to the research community. iMedRIS offers a pre-configured system that can be completely customized to institutional needs providing a framework application that empowers institutions with intuitive tools needed to optimize research operations by eliminating inefficiencies and increasing productivity. Some of our core iRIS™ modules include IRB Assistant™, IBC Assistant™, IACUC Assitant™, COI Assistant™, eProposal Assistant (pre-award).

InfoEd Global 5 Washington Square Suite 2 Albany, NY 12205 Phone: 518-713-4200 www.infoedglobal.com InfoEd Global Is the World’s Leading Provider of Software to Support Electronic Research Administration. Worldwide, more leading research institutions, universities, and corporations have chosen the InfoEd Solution to realize cost savings, improve data integrity, streamline administrative processes, and accelerate compliance, than any other eRA software. At InfoEd Global we are committed to providing the best eRA solutions for the world’s best research institutions.

Innovive 10019 Waples Court San Diego, CA 92121 Phone: 866-432-2437 Fax: 858-309-6621 www.innovive.com Innovive is the leading manufacturer of high-performance IVC rodent housing, designed for disposable caging. Our patented IVC racks and caging system exceed industry standards for performance and durability; they also enable research animal facilities to eliminate/reduce traditional cage wash operations. With over 300 installations worldwide, we continue to serve the animal research community in the effort towards scientific discovery.

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Advancing pre-clinical oncology with thermography and 3D scanning of subcutaneous tumors. BioVolume is the world’s first 3D imaging solution that has been developed in partnership with a leading pharmaceutical company and wider industry for visualizing and measuring subcutaneous tumor growth in preclinical oncology research.

It was conceived to enable faster, smarter, and more confident decisions to be made in the identification and development of new cancer therapeutics.

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EXHIBITORS & SPONSORS Instech Laboratories, Inc. 450 S Gravers Road Plymouth Meeting, PA 19462 Phone: 484-576-2362 www.instechlabs.com Instech designs and manufactures 3Rs-friendly products for rodent infusion, sampling and oral gavage, including catheters, tethers, Vascular Access Buttons™, swivels, infusion pumps, automated blood samplers and animal feeding tubes. Be nice.

Institute for Laboratory Animal Research 500 Fifth Street, NW Washington, DC 22310 Phone: 703-687-7045 www.dels.nas.edu/ilar For more than 60 years, ILAR has provided science-based guidance for the responsible use, care, and welfare of animals in research and testing in the United States and abroad. ILAR’s Guide for the Care and Use of Laboratory Animals serves as the basis for accreditation of institutions worldwide.

Working in partnership with the Oxford University Press, ILAR publishes the ILAR Journal—the only quarterly peer-reviewed publication specifically for all those who oversee laboratory research animals.

Institute of Animal Technology 5 South Parade Oxford OX2 7JL United Kingdom Phone: +448000854380 www.iat.org.uk Representing professional animal technologists both within the United Kingdom and around the world, the IAT has over 2400 members, which includes the vast majority of the UK’s Named Animal Care and Welfare Officers (NACWOs) and operates a register of animal technologists (RAnTech).

If you have any queries, please contact us at admin@iat.org.uk International Council for Laboratory Animal Science Vapshcs (151-Research) 1660 S. Columbian Way Seattle, WA 98108 Phone: 206-764-2448 Email: info@iclas.org www.iclas.org ICLAS is an international scientific organization dedicated to advancing human and animal health by promoting the ethical care and use of laboratory animals in research worldwide, and particularly in developing countries. ICLAS promotes international collaboration; quality definition and monitoring of animals; harmonization; humane use through recognition of ethical principles, scientific responsibilities, and the 3Rs of Russel and Burch.

Intuitive Biosciences 918 Deming Way, Suite 100 Madison, WI 53717 Phone: 608-561-8730 intuitivebio.com/services/veterinary-diagnostic-services/ Our goal is to reduce your workload by providing serology testing services and kits to support your SPF colony of nonhuman primates. In your lab our ours, we provide rapid and accurate results. Our easy-to-use platform screens NHP for infectious diseases. Our patented CSA: Simian TB assay provides reliable and fast results for exposure to M. tuberculosis. Working on SARS-CoV-2?

We have serology tests for antibody and titers, and neutralizing antibody characterization. How can we help you?

Iwaki Aquatic 330 Hopping Brook Road Holliston, MA 01746 Phone: 508-474-2052 www.iwakiaquatic.com Iwaki Aquatic engineers life support systems, dosing systems and aquatic housing systems. Our aquatic team consists of aquaculture specialists, biologists, engineers and customer service experts with many years of experience supporting aquatic research systems. We specialize in Zebrafish and Xenopus but over the years our experts have designed and built custom systems for over 150 different aquatic organisms. Visit booth 213 to discuss your next project or service needs for your existing systems.

Janvier Labs Route des Chênes Secs Le Genest Saint Isle 53940 France www.janvier-labs.com Janvier Labs is an international player in biomedical research that supplies experimental models with the highest standards and solutions adapted to meet specific needs at each stage of the research projects, from the creation of your model to the delivery to your laboratory.

Lab Products, Inc. 742 Sussex Ave. Seaford, DE 19973 Phone: 302-628-4300 www.labproductsinc.com Lab Products, Inc. is a leading supplier of animal caging and handling systems for biomedical research facilities. We offer an extensive line of equipment including the Micro-Isolator™, ventilated caging systems, work benches, waste management, NHP Units, kennels and numerous accessories and environmental control products. Lab Products, Inc. also provides a disposable caging system as well.

Lab Supply 54 Remington Dr. Highland Village, TX 75077 Phone: 817-422-8461 Fax: 682-237-2363 www.labsupplytx.com Lab Supply provides a complete line of products for lab animal research facilities which includes animal diets, beddings, enrichment items, disinfectants, and personal protection equipment (PPE). With 4 climate-controlled warehouses (DFW, Orlando, Durham, and Houston), we can service customers coast to coast for 30+ years. During this 2020 pandemic, our motto was put to the test and proven true: “When you’re all-in with Lab Supply, we guarantee you will never run out of product.”

LabDiet 100 Danforth Dr. Gray Summit, MO 63039 Phone: 800-227-8941 Fax: 651-234-8505 www.labdiet.com Purina Mills is the International supplier of the LabDiet® and TestDiet® products used in lab animal nutrition. All products are manufactured in our ISO9001:2000 Certified plant in Richmond, IN. Diets are available in Standard, Certified, Autoclavable, Irradiated, Vac-Pak and Micro-Pak forms. Custom diets are also available upon request. For complete nutritional composition of our diets, visit www.labdiet.com and www. testdiet.com .

Laboratory Animal Breeders Assn 251 Ballardvale St Wilmington, DE 01887 Phone: 781-222-6370 Fax: 978-988-5827 Laboratory Animal Management Association 1161 Wayzata Blvd E #247 Wayzata, MN 55391 Phone: 612-619-6437 www.lama-online.org The Laboratory Animal Management Association was founded in 1984. It provides a professional focus for individuals who serve as directors, managers, and supervisors of animal laboratories. The objectives are to promote the dissemination of ideas, experience and knowledge; to encourage continued learning; to act as a spokesperson, and to assist management training.

Laboratory Animal Welfare and Training Exchange 401 North Middletown Rd B200, RM1002 Pearl River, NY 10965 Phone: 845-602-2462 www.lawte.org The Laboratory Animal Welfare and Training Exchange (LAWTE) is a global organization which aims to enhance animal welfare through the support of quality training programs and the development of training professionals in the laboratory

EXHIBITORS & SPONSORS Laboratory Animals Limited P O Box 373 Eye Suffolk IP22 9BS United Kingdom Phone: 00441449720881 www.lal.org.uk Laboratory Animals Limited [LAL] is a registered charity that is dedicated to the advancement of all aspects of laboratory animal science, technology and welfare achieved through supporting the 3Rs in education and training. LAL believes animal experimentation must only be performed when considered necessary and all work must conform to the highest ethical standards.

Lenderking Caging Products 8370 Jumpers Hole Rd Millersville, MD 21108 Phone: 410-544-8795 Fax: 410-544-8795 www.lenderking.com Lenderking Caging Products is a manufacturer of a complete line of high quality and innovative animal housing for the pharmaceutical research industry. We create products that offer solutions to typical problems found in the laboratory environment. “It takes America’s Oldest Caging Company to bring you the Newest Innovations.”

LGL Animal Care Products, Inc. 721 Peach Creek Cutoff College Station, TX 77845 Phone: 979-690-3434 www.lglacp.com LGL Animal Care Products, Inc. is a trusted and recognized leader in innovated design, manufacturing and installation of high quality, cost-effective custom animal cages used in veterinary clinics, animal control facilities, zoos and research laboratories around the world. Ease of cleaning, visibility and animal comfort is always our priority.

Life Science Products, Inc. 124 Speer Road Chestertown, MD 21620 Phone: 410-778-6474 www.lspinc.com From Personal Protection to Seamless Hygienic Interior Finishes, Life Science Products Inc. has been creating customized solutions for all Science and Technology spaces for well over 4 decades. We have both product and installation solutions for PPE, (and related equip.) UV cured flooring, crash rail, doors, composite walls, composite ceilings and high-performance coatings.

Creating a better, more secure envelope is our mission. Let us help you with your solution.

Lighthouse Life Science Partners, LLC 100 Trade Center Drive, Suite G-700 Woburn, MA 01801 Phone: 800-298-5433 Fax: 952-469-9490 www.lighthouselifesciences.com Evidence-based cleaning & disinfecting programs for your research facility. We offer a portfolio of best-in-class cleaning and disinfecting technologies and tools. Please stop in and ask us about our technologies and clients results – including facilities that have not experienced an outbreak since implementation and improved efficiencies in their cleaning and disinfecting processes.

Marshall Bioresources 5800 Lake Bluff Rd North Rose, New York 14516-9795 Phone: 315-587-2295 Fax: 315-587-2109 infous@marshallbio.com www.marshallbio.com Our mission is to be the premier source of animals and related services for biomedical and veterinary research. Our animal models include conventionally raised and APD/SPF Marshall Beagles® and Cats, Influenza-free Marshall Ferrets®, Mongrels/Hounds, IFN knockout Mice (AG 129 & A 129), SPF guinea pigs, and Gottingen Minipigs® in North America.

Lithgow Laboratory Services 6955 N Durango Drive #1115-138 Las Vegas, NV 89149 Phone: 702-413-0832 Fax: 702-363-4981 www.lithgowservices.com Lithgow Laboratory Services (LLS) is dedicated to serving our customers’ needs with a represented line of ventilated caging, washers, PPE, floors, walls, ceiling products, & CAPR systems. Founded in 1962 LLS is a long-standing member of the AALAS community and is well known for our customer service. With our represented companies we can outfit your facility from the floors up.

Contact us at 702-413-0832 or sales@lithgowservices.com or visit our website www. lithgowservices.com

Medline Industries, Inc. Three Lakes Drive Northfield, IL 60093 Phone: 847-837-2814 Fax: 123-456-7890 www.medline.com/educationandresearch Medline is a global manufacturer and distributor serving the healthcare industry with medical supplies and clinical solutions that help customers achieve both clinical and financial success. Headquartered in Northfield, Ill., the company offers 550,000+ medical devices and support services through more than 1,600 direct sales representatives who are dedicated points of contact for customers across the continuum of care. For more information on Medline, go to www.medline.com or www.medline. com/social-media to connect with Medline on its social media channels.

Lomir Biomedical Inc. President Sponsor 95 Huot N-D-Ille-Perrot QC J7V 7M4 Canada Phone: 514-425-3604 Fax: 514-425-3605 www.lomir.com Lomir is the world's largest manufacturer of animal jackets, infusion products, restrainers and more. In depth knowledge enables Lomir to design and manufacture equipment with the exact precision to meet your objectives. Continued innovation in manufacturing and materials allow researchers to consider new applications and refinement with animal welfare and well-being driving the process. Visit us in our virtual booth to learn more and benefit from working directly with the manufacturer.

MAI Animal Health 605 Pro-Ject Drive Elmwood, WI 54740 Phone: 715-953-2415 www.maianimalhealth.com MAI Animal Health™ introduces the Vetcorder™ Pro, an affordable device to allow patient monitoring of ECG, SpO2 and Internal Temperature. The monitor can be mirrored wirelessly for viewing from a distance. We are THE SOURCE for solutions in animal healthcare supplying innovative products spanning multiple veterinary disciplines for over 40 years.

Mispro Biotech Services 450 E. 29th St, Suite 602 New York, NY 10016 Phone: 781-254-2178 misprobiotech.com Mispro Biotech Services offers biopharmaceutical and life sciences companies access to coworking vivarium research space for preclinical drug development. Mispro supports its clients with comprehensive husbandry and regulatory oversight services and has locations in key biotech hubs in the U.S. and Canada.

National Association for Biomedical Research 1100 Vermont Ave, Suite 1100 Washington, DC 20005 Phone: 516-660-4726 www.nabr.org The National Association for Biomedical Research (NABR), founded in 1979, is the nation's only organization dedicated solely to advocating sound public policy that recognizes the vital role animals play in biomedical research. NABR provides a unified voice for the scientific research community on legislative and regulatory matters in support of ethical and essential laboratory animal research.

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animal science community. Our objectives are to promote information and knowledge exchange among training professionals on training programs, systems, materials and services through its website, conferences, webinars, and electronic listserv.

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EXHIBITORS & SPONSORS New England Ovis 225 Rollins Road Rollinsford, NH 03869 Phone: 6037811149 www.neosheep.com New England Ovis SPF sheep are cesarean-derived sheep and free of over 50 pathogens, including Q-Fever. Rigorous biosecurity and surveillance maintain unparalleled health status. NEO STARRS socialization program results in calm, tame, and well-acclimated animals. All ages, weights, genders, time-dated pregnant ewes available year-round. Transportation and training available.

North American 3Rs Collaborative 2745 Poplar Gap Road Hot Springs, NC 28743 Phone: 919-608-9550 www.na3rsc.org North American 3Rs Collaborative facilitates communication and sharing among members and like-minded organizations to bring transformational solutions that advance science and animal welfare. The website is a hub of information and collaboration for the scientific community looking for details about the 3Rs in North America. Please visit and become active in our organization!

NuAire Laboratory Equipment 2100 Fernbrook Lane Plymouth, MN 55447 Phone: 763-553-1270 Fax: 763-553-0459 www.nuaire.com NuAire is committed to bringing you the highest-quality, most dependable products.

We are recognized as one of the world's leading providers of reliable equipment, including Biosafety Cabinets, Animal Transfer Stations, Animal Refuse Workstations, and more. You can depend on our products to feature brilliant but practical design. As a customer, you can rely on outstanding value and dependable service - the cornerstones of our reputation.

Oak Hill Genetics, LLC 21434 Oak Hill Road Ewing, IL 62836 Phone: 618-629-2692 www.oakhillgenetics.com Oak Hill Genetics is a family owned and operated company dedicated to providing the research world with high-quality animal models. Our purpose-bred hounds and swine are born and raised in Class A facilities in southern Illinois. We also have a new location in central California serving the west coast. Our mission and passion is healthy, happy research animals and premiere customer service.

Pharmacal Research Labs, Inc. 562 Captain Neville Drive Waterbury, CT 06705 Phone: 203-755-4908 Fax: 203-755-4309 www.pharmacal.com Pharmacal Research Labs, Inc. is the leading manufacturer and supplier of high-quality sanitation products, designed, researched, and tested for laboratory animal research facilities. Our extensive line of sanitation chemicals, bio-safety level disinfectants, custom designed chemical delivery systems and service program exceed industry standards for efficacy and safety.

Quip Laboratories, Inc Gold Sponsor 1500 Eastlawn Avenue Wilmington, DE 10312 Phone: 302-357-8065 www.quiplabs.com From our unique decontamination, chemical dispensing and storage, monitoring and lab services, to our proprietary disinfectants and sterilants, Quip Laboratories provides a comprehensive approach to efficient and eco-gentle facility hygiene. You can find Quip Labs on the web at www.quiplabs.com, or call us today at 1-800-424-2436!

Plas-Labs, Inc. 401 East North Street Lansing, MI 48906 Phone: 517-372-7177 www.plas-labs.com Since 1967, Plas-Labs has manufactured animal handling and research equipment for laboratories, research facilities and veterinarians all over the world. With over 100 distributors worldwide, the fine line of Plas-Labs' products is always available. The new distribution center in Europe offers quicker and cheaper shipping for researchers in Europe. Plas-Labs' products include small handling equipment, intensive care units, primate chairs, surgery equipment, and isolation glove boxes.

RICA Surgical Products, Inc. 9207 Ivanhoe Street Schiller Park, IL 60176 Phone: 952-215-8722 Fax: 847-671-5690 www.ricasurgical.com RICA Surgical Products is your source for high quality surgical products. RICA represents the leading surgical instrument and time-saving device product brands such as KMedic®, Pilling® Weck, RICA® instruments; Weck® Hemoclips® and Visistat® skin staplers. See these quality products along with RICA's patented Thermal Controlled Pads to effectively manage/prevent hypothermia and their Paw Protector® Cage Racks to keep animals warm & comfortable. Fantastic special offers will reward your visit.

PreLabs 5781 Lee Blvd #208-217 Lehigh Acres, FL 33925 Phone: 847-613-8386 www.prelabs.com PreLabs is a preclinical research support organization providing in vivo and in vitro services in multi-species with an emphasis on nonhuman primate (NHP) models.

Our PrimGen division offers sourcing and importation of various NHPs, including Rhesus, Cynos and African Green Monkeys, as well as distribution, quarantine, holding, logistics and biological services supported

by AAALAC accredited regional distribution centers, AALAS certified staff and comprehensive data management capabilities.

Robinson Services Inc. PO Box 1057 Mocksville, NC 27028-1057 Phone: 336-940-2550 Fax: 336-940-5260 www.rsirabbits.com Robinson Services Incorporated (RSI) has been supplying the research community with animal models since 1986. We currently provide SPF rabbits, healthy and robust guinea pigs as well as Q Fever negative sheep and goats. We are the lowest cost provider of these models in the industry and provide door to door delivery. Our clients include the biggest CROs and Toxicology facilities in the US as well as a host of clients in academia. Call us and let us show you how we can drive your animal costs down!

PVS Process Equipment 2600 Hamburg Turnpike Buffalo, NY 14218 Phone: 716-646-0181 www.pvspe.com PVS Process Equipment specializes in the design, development, and manufacturing of bedding handling systems. Focusing on bedding delivery, dispensing and disposal. With some of the most cutting-edge innovations, PVS is a market leader when it comes to the latest products for supplying clean bedding and dispensing it.

S&S Farms DBA Premier BioSource 1650 Warnock Drive Ramona, CA 92065 Phone: 760-788-7007 www.premierbiosource.com Premier BioSource (formerly S&S Farms) has specialized in the production of high health status pigs since 1987. Our goal is to provide our customers with the highest quality swine model. Premier BioSource offers both domestic pigs (farm pigs) and Yucatan minipigs. Both models are bred in southern California and northern Indiana.

They are available throughout the United States and Canada.

EXHIBITORS & SPONSORS

Sarstedt PO Box 468 Newton, NC 28658 Phone: 828-465-4000 www.sarstedt.com Sarstedt is a worldwide provider of laboratory consumables, specimen collection products, and medical devices. We offer a range of blood collection systems from micro to large volumes, as well as products for specimen transport, liquid handling, and general laboratory use. Ask about free samples!

Scientists Center for Animal Welfare (SCAW) 2660 NE Hwy 20, Suite 610-115 Bend, OR 97701 Phone: 301-345-3500 www.scaw.com The Scientists Center for Animal Welfare (SCAW) is dedicated to balancing animal welfare and excellence in science through educational outreach.

SenSV 8 Penn Center, 1628 JFK Boulevard Philadelphia, PA 19103 Phone: (215) 248 4352, ext 102 f.doggett@metro-acoustics.com www.metro acoustics.com SenSV supports peak research performance through productivity; it can be accessed anytime, anywhere. SenSV monitors for micro-vibration, audible sound, and ultrasonic sound, allowing the lab to make informed decisions for continuity and reproducibility. It converts raw data to 1/3 octave band RMS levels to compare to industry-standard thresholds, and includes a real-time alert system and a print-to-report function.It also allows for quicker identification of critical issues, leading to less stress for animals and less downtime, reducing the need for repeated testing, and providing more successful outcomes with fewer errors.

Shepherd Specialty Papers 112 Tennessee Blvd.

Watertown, TN 37184 Phone: 781-826-1581 www.ssponline.com Shepherd Specialty Papers has been committed to provide a full line of quality products to the animal care industry for over 30 years. We offer a variety of Cage and Pan Liners, Bedding, and Environmental Enrichment items providing the best possible animal care while making cage maintenance easier and more economical. Our patented Shepherd Shack® and Enviro-dri have revolutionized rodent enrichment. Utilizing years of material handling knowledge, we offer a comprehensive bedding dispensing system.

Sinclair Bio Resources, LLC 562 State Road DD Auxvasse, MO 65251 Phone: 573-387-4240 www.sinclairbioresources.com Sinclair Bio Resources is an AAALAC-accredited, USDA-inspected/licensed miniature swine producer offering four lineages of miniature swine: Sinclair, Yucatan, Micro Yucatan, and Hanford. Sinclair is experienced in pre-shipping alterations, including surgical alterations, pre-shipment conditioning and training, and diabetic induction. Sinclair also has a unique behavior enhancement system resulting in the most docile, easy-to-work with miniature swine available. Contact us with technical questions and experience our industry leading client service!

SMC-Roe Audubon Machinery Corp. 814 Wurlitzer Drive North Tonawanda, NY 14120 Phone: 716-564-5165 www.smc-roe.com Roe Bio-Medical joined Schlyer Machine (SMC) in 2012 with the continuing goal of providing Everything for the Vivarium Washroom. Schlyer Machine has been manufacturing quality cage washing equipment for the Life Sciences Research field for over 25 years. Roe Bio-Medical Products has been providing Vacuum Bedding Delivery & Removal Systems to the Biomedical Research field since 1994. SMC-Roe’s operations are located in N. Tonawanda, NY, just a few minutes from Niagara Falls.

Somark 6861 Nancy Ridge Dr San Diego, CA 92121 Phone: 800-430-1243 Email: earlund@somarkinnovations.com mysensalab.com Somark created the Digital MouseTM utilizing the world’s smallest and most affordable medical research RFID Tag. You can now track any mouse, anywhere, in real time.

Visit us at mysensalab.com.

Spire Integrated Solutions Silver Sponsor 8719 S 135th St, Ste #300 Omaha, NE 68138 Phone: 402-344-4200 www.spire-is.com Spire Integrated Solutions, a Chalmers Group Company, is a family of brands that produces world-class steam sterilizers, a full range of washing equipment, refrigeration products, and ancillary stainless-steel equipment. Built with non-proprietary parts, Spire solutions simplify maintenance and ensure ease of use for laboratories, hospitals, biopharmaceutical producers, morgues/mortuaries, and other life science and healthcare facilities. Spire also provides flexible service and training programs to reinforce equipment uptime and longevity while lowering the total cost of ownership.

For reliable solutions that can build strength in your facility, be confident with Spire equipment at your side. ssniff Spezialdiaeten GmbH Ferdinand-Gabriel-Weg 16 Soest 59494 Germany Phone: +49 2921 965813 Fax: +49 2921 965840 www.ssniff.de ssniff offers a range of products including standard, autoclavable, and gamma-irradiated feeds for all laboratory species, plus specialty lines covering purified diets, medicated feeds, test-compound diets, and formulations for pre-clinical studies (incl.

PK/tox/GLP studies) with batch sizes as low as 1 kg.

Suburban Surgical Co Inc. 275 12th Street Wheeling, IL 60090 Phone: 847-537-9320 Fax: 847-537-9061 www.suburbansurgical.com For more than 65 years and three generations, Suburban Surgical Inc., an industrial manufacturer, has designed, engineered, and fabricated the highest quality stainless steel equipment available for the life sciences market. Our fine reputation for aggressive design concepts and quality workmanship has made us a leader in the industry. Made in Wheeling, IL. Sychem 3 Mayflower Close Chandlers Ford, Hampshire SO53 4AR United Kingdom Phone: 08456446824 Email: tim@sychem.co.uk Systems Engineering 932 Kaiser road Napa, CA 94558 Phone: 707-253-8852 www.selabgroup.net For 50 years Systems Engineering has sup-

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SAI Infusion Technologies 278 Park Ave Lake Villa, IL 60046 Phone: 847-356-0321 www.sai-infusion.com SAI creates components for preclinical infusion and sampling. We provide technical support and training for all our products, including on-site infusion set up. Improve your study outcomes with customizable equipment including harnesses, access ports, tethers, jackets, swivels, and catheters, as well as the most flexible pumps on the market. Our focus is on helping animal researchers get work done faster and better, so your focus stays where it belongs- on saving human and animal lives.

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EXHIBITORS & SPONSORS ported all animal species and cage designs used by all caging manufacturers. The industry’s first choice is the L-2000™ bedding resistant water valve. Systems Engineering provides a design build animal recirculating water system. Providing excellent customer service, Systems Engineering also offers a superior IVC valve and disaster planning and hydration through the Napa Nectar™ water packs.

Taconic Biosciences 1 Discovery Drive, Suite 304 Rensselaer, NY 12144 Phone: 518-697-3900 www.taconic.com Taconic Biosciences is a fully licensed leader in genetically engineered rodent models and services. Taconic helps researchers acquire, generate, breed, test, and distribute research models worldwide.

Tecniplast Gold Sponsor 1345 Enterprise Dr West Chester, PA 19380 Phone: 484-947-8449 Fax: 484-947-0511 www.tecniplastusa.com Tecniplast has more than 70 years of experience in the design and manufacture of products for the laboratory animal industry.

Our Products: IVC and digital housing NHP Housing Macaque, Marmosets, and Group housing. Iso Positive & Iso Negative caging & Safety Cabinets. Changing Stations Aquatics Housing and feeding systems Washing - High-efficiency rack, cage, cabinet, and tunnel. Automation clean and dirty side Tecniplast USA manufacturing plant is in West Chester, Pennsylvania.

Thai Association For Laboratory Animal Science 5205 Chairman’s Court, Suite 300 Frederick, MD 21703 Phone: 301-696-9626 Fax: 301-696-9627 talasthailand.org The Thai Association For Laboratory Animal Science (TALAS) represents Thai research animal community at national and international levels; promotes the ethical and responsible use of research animals; sustains essential knowledge and continuing education regarding contemporary laboratory animal sciences; and leads collaborative networking for Thai research community.

The Andersons Lab Bedding Products PO Box 119 Maumee, OH 43537 Phone: 419-891-6423 www.andersonslabbedding.com The Andersons premium product offering includes a broad selection aimed at making an unnatural environment more natural with the highest quality bedding, nesting, and enrichment products. From irradiated

and qualified products available in autoclavable and bulk bags, to sterility and heavy metal testing, we provide several services to the industry in accordance with researchers’ desires for the highest quality products for their animals and studies.

The Baker Company 175 Gatehouse Road Sanford ME 04073 Phone: 800-992-2537 bakerco@bakerco.com www.bakerco.com Baker’s high-performance Animal Laboratory Equipment is built with you in mind, with industry-leading ergonomics, energy-efficient engineering, and the lowest life cycle costs available. Be sure to check out all our new innovative options and configurations that help you work more comfortably, boost productivity, save money, and minimize environmental impact.

The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 Phone: 207-288-1431 www.jax.org The Jackson Laboratory is an independent, nonprofit biomedical research institution, with a National Cancer Institute-designated Cancer Center and more than 1,500 employees. With locations in Bar Harbor, Maine, Sacramento, Calif., and a new genomic medicine institute in Farmington, Conn, its mission is to discover precise genomic solutions for disease and empower the global biomedical community in the shared quest to improve human health.

Thoren Caging Systems, Inc. 815 West 7th Street Hazleton, PA 18201 Phone: 570-455-5041 www.thoren.com Thoren is the industry leader in providing the research community with IVC rodent housing, aquatics, and euthanasia racks.

Thoren: tried, true, and trusted for over 66 years.

TOPAZ Technologies 3820 Mansell Rd. #375 Alpharetta, GA 30022 Phone: 678-982-4712 topazti.com TOPAZ Technologies, LLC provides animal and human research software solutions, and has been the industry's leading vendor for nearly 40 years. Our suite of integrated applications enables automated workflows from protocol reviews to staff training, animal procurement to census, billing, and veterinary care. We will help you maintain regulatory and AAALAC International compliance, provide your research community customers with low overhead costs,

improve the job satisfaction and retention of your facility staff, and enhance the quality of animal care.

Transnetyx President Sponsor 8110 Cordova Road, Suite 119 Cordova, TN 38016 Phone: 901-338-9825 www.transnetyx.com Transnetyx genetic services (Automated Genotyping, Genetic Monitoring, Microbiome Analysis, Transnetyx Tissue) and colony management software (Transnetyx Colony) allow labs and facilities to focus on efficiently and responsibly progressing research forward while effectively saving valuable time and resources. Together we look to make a significant impact on research through discovery!

Turner Scientific LLC Silver Sponsor 1351 Lincoln Ave. Jacksonville, IL 62650 Phone: 217-602-0306 www.turnerscientific.com Turner Scientific brings 20 years of experience in animal facilities to work for you as a full-service monitoring firm. The result is that environmental stimuli can be confounds for ANY area of biomedical research using animal models because they add variability to measurements, resulting in greater statistical ambiguity in our research and the need for more animals. We offer products and services to monitor and measure these confounds to better all research.

UID Identification Solutions 500 Park Ave Ste 109 Lake Villa, IL 60046-6550 Phone: 224-444-8484 Fax: 847-356-0382 www.uidevices.com UID provides a secure method for the identification of animals and other laboratory items using implantable and externals RFID transponders. The UID Identification system includes RFID implantable temperature transponders for mice, rats, and large animals. We will also demonstrate our RFID labels, readers, programmers, and software for laboratory automation processes. In addition, we will showcase an RFID-enabled controlled substance cabinet for monitoring and tracking all narcotics volumes and inventories for 100% accurate records all collected automatically.

Verona Safety Supply 913 Watson Avenue Madison, WI 53713 Phone: 608-273-3520 www.veronasafety.com Verona Safety is a total safety solution provider. We have partnered ourselves with the industry's leading manufacturers

EXHIBITORS & SPONSORS

VetEquip 4777 Bennett Dr Ste E #303 Livermore, CA 94551 Phone: 925-463-1828 www.vetequip.com VetEquip's reputation for trust and reliability continues to dominate the market in inhalation anesthesia delivery and pollution control. We have the widest range of products to meet the individual needs of your laboratory. An unconditional 7-year warranty gives you peace of mind that VetEquip is always the best choice.

VRL Laboratories 7540 Louis Pasteur Dr. Suite 200 San Antonio, TX 78229 Phone: 630-247-1342 www.vrl.net A high-quality animal health program demands high-quality results. VRL offers a full catalog of diagnostic services for rodents, rabbits and Non-human primates backed up by our scientific staff. At VRL, our priority is to ensure our clients accessibility to the highest level of expertise and customer service in the industry. We are dedicated to providing you non-biased high-quality results with fast turnaround times and unmatched customer service.

W.F. Fisher & Son 220 Evans Way, Suite 1 Somerville, NJ 08876 Phone: 908-707-4050 Fax: 908-707-4099 www.wffisher.com W.F. Fisher & Son is a small, family-owned and operated Certified Lab Dealer that has been delivering high quality products and services to the research industry of NJ, NY and CT since 1955. We are the first distributor of laboratory animal feed and bedding in the country to be ISO 9002 Certified.

W.F. Fisher & Son are proud members of NJAALAS, MNYBAALAS, SNEAALAS, National AALAS, NJABR, and LAMA. Featured products include the innovative, portion-controlled items - Enviropak and Nestpak.

Wedgewood Pharmacy 405 Heron Dr Swedesboro, NJ 08085 Phone: 800-331-8272 Fax: 800-589-4250 www.wedgewoodpharmacy.com/ Great care, delivered. That is the Wedgewood Pharmacy promise to you and your clients. Over 80% of the nation’s veterinarians enjoy our fast, free shipping, industry-leading digital tools, dedicated account management team, rigorous quality standards backed by a team of experts, and an extensive formulary of over 40,000 preparations.

XpressBio 4650 Wedgewood Blvd, Suite 103 Frederick, MD 21703 Phone: 301-228-2444 www.xpressbio.com XpressBio markets molecular biology products to the bioscience research community.

Our customers are some of the leading pharmaceutical, biotechnology, government, and academic organizations around the world.

Zebrafish Husbandry Association 1 Blackfan Circle Boston, MA 02115 www.zhaonline.org The Zebrafish Husbandry Association (ZHA) is a nonprofit organization devoted to promoting and developing zebrafish husbandry standards through education, collaboration, and publication. ZHA's membership is comprised of a wide range of people interested in zebrafish culture, including research and veterinary professionals, facility managers, technicians, fish culturists, aquaculture engineers, and representatives from various private aquaculture supply and biomedical companies.

ZooPharm-SR Veterinary Technologies 1230 West Ash Street, Suite D Windsor, CO 80550 Phone: 970-795-0920 www.zoopharm.net SR Veterinary Technologies specializes in the controlled release of pharmaceuticals for pain management, anesthesia, and other research applications. Through its patented, fully biodegradable liquid polymer matrix, the system provides consistent release of actives for 72+ hours. SR formulations require lower dosing frequency and can be injected subcutaneously through needles as small as 23-gauge.

Non-Exhibiting Sponsors Bristol Myers Squibb President Sponsor

ASAP Gold Sponsor Merck Gold Sponsor Sanofi Silver Sponsor

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of Personal Protective Equipment (PPE), Work Wear, Plant Safety, and Emergency Response items to provide our customers with the finest product selection and services available. Our philosophy is that the customer is always number one; therefore, we are committed to providing unparalleled customer service, top quality products, and great prices.

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TECHNICAL TRADE PRESENTATIONS

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TRACK I - CAPITALIZING DATA TECHNOLOGY Airports Don’t Use Pen and Paper Anymore, Nor Should Animal Facilities. Insights for Animal Facility Management Solution Planning u

Speaker: Eric Y Rieux Moderator: TBN

What is the difference between an animal facility and an airport? Both must screen, move, trace, and keep their clients and “subjects” happy. This means ensuring they are all delivered safely, on-time, and to the right place. Both must reliably manage operations of buildings and maintain environmental conditions under strict controls while ensuring that all critical supplies are stocked. Both must ensure that staff have the right training, qualifications, and are assigned to the right task (don’t confuse the pilot with the janitor!). Both operate with many key performance indicators (occupancy rates, services on-time, etc.) and under strict regulatory compliance. Both must be able plan and react to the unplanned and to alerts. And finally, both face regular changes and must be able to adapt and evolve.

Just as airports no longer operate with paper records, animal facilities are more than ever relying on software tools to manage it all. And just as two airports are never the same, each animal facility is unique. This talk will make the case that no one solution for animal facility management is perfect and encompassing. Each unique case requires a solution that fits. Participants will learn how to put together a basic User Requirements Specification (URS), what questions to ask internally and externally when searching/sourcing a solution, which colleagues and function to include and enlist from the get-go, and how to get buy-in to increase chances of being funded. The target audience includes research directors, lab/facility/ operations manager, veterinarians, and last but not least, the people who get things done, zoo technicians.

This Technical Trade Presentation is sponsored in part by Brain & Software International (BSI)

Use of Automated Blood Sampling to Improve Animal Welfare and Data Collection for Large Animal Studies

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Speaker: Candace Rohde-Johnson Moderator: Shelly Carballo

Automating blood sampling in large animal models can improve translatability and reproducibility for preclinical drug development. This is achieved through several mechanisms including sampling from animals with reduced stress, combining study protocols to incorporate both pharmacokinetics (PK) and pharmacodynamics (PD) in a single animal, and the ability to collect samples from free moving animals, even during overnight periods. Not only does automation lead to better data, but it also makes it possible to reduce overall animal numbers. This presentation will review studies and publications by researchers who have refined their studies using the Culex-L automated blood sampling system for large animals. Further, the presentation will outline the simple steps and materials you need to incorporate automated sampling into your studies.

This Technical Trade Presentation is sponsored in part by BASi Research Products.

Using 3D Scanning to Improve the Measurement and Analysis of Subcutaneous Tumors

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Speaker: Andrew Smith Moderator: Karl Turley

We will discuss the benefits using a 3D and thermal scanning system to monitor the development of subcutaneous tumors in in-vivo oncology research. Providing an overview of how utilizing the latest capture technologies, machine learning and data science are providing a wide range of benefits. Improving data traceability, scientific confidence, study repeatability and animal welfare.

This Technical Trade Presentation is sponsored in part by Fuel3D. u Dry Heat Sterilization in Vivariums: An Alternative Option for Sterilization in the Lab Animal Facility

Speaker/Moderator: Robert C Davis The presentation focuses on the use of dry heat instead of steam for the sterilization of laboratory animal cages, IVC racks, enrichment, and other items used in the vivarium. Topics to be covered include the development of dry heat sterilization in laboratory animal facilities; what is dry heat sterilization and how does it work with laboratory animal cages, IVC racks and related items; how the dry heat sterilizer is configured; the benefits and limitations of dry heat sterilization; recent case studies showing specific customer challenges and how dry heat sterilization solved them; how dry heat sterilization compares to steam sterilization; and how these systems are validated.

This Technical Trade Presentation is sponsored in part by TPS-Gruenberg.

Increase the Ease and Speed of Decontaminating Isolators: Explore Modular Hybrid Hydrogen Peroxide u

Speaker: Frances M. Grinstead Moderator: Rich Mullen

Decontaminating gnotobiotic chambers and the spaces they inhabit takes enormous amounts of time and labor, severely hampering the turnaround time for routine disinfection. The current process delays the delivery of food, bedding, and supplies to the chamber in addition to the commencement of new studies.

Traditionally, decontamination treatments involve labor-intensive disassembly of soft chambers, discarding any HEPA filtration and spraying with caustic chemical sterilants like peracetic acid and chlorine dioxide. On average, it takes 3 days for chemically treated chambers to dry out completely and become reusable. While immersion sterilization is well documented to achieve spore sterilization, the process treats only removable items and efficacy in filtration systems is unknown. Increasing the ease and speed of the gnotobiotic decontamination process would greatly enhance research capabilities, allowing scientists to focus more on their studies than manual labor. This presentation explores modular hybrid hydrogen peroxide (HHP) fog technology as a faster, safer alternative to conventional protocols. Participants will understand how combining aerosol and vapor hydrogen peroxide creates HHP capable of a 6-log reduction of spores in the most challenging locations within a closed system in just over an hour. We will examine the challenges and results of performing HHP decontamination of gnotobiotic chambers, their contents, delivery sleeves, and laboratory spaces. Discover how the TRINITY HHP process is key to achieving spore inactivation with low-level 7% hydrogen peroxide while decreasing decontamination time by days and reducing the man hours necessary to successfully result in a 99.9999% reduction of pathogens. This presentation is ideal for facility directors, veterinarians, and researchers in charge of maintaining the integrity of the research environment, from whole-laboratory spaces to individual gnotobiotic chambers.

This Technical Trade Presentation is sponsored in part by CURIS System.

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TECHNICAL TRADE PRESENTATIONS

This Technical Trade Presentation is sponsored in part by Lighthouse Life Sciences.

This Technical Trade Presentation is sponsored in part by ClearH2O.

Speaker: Nicole Kenny Moderator: Lara Storrar

TRACK II – RESEARCH MODELS AND FACILITY DESIGN All Hot and Not Bothered: A Novel Way to Provide Thermal Comfort to Mice

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Speaker: Beverly A Chua Moderator: Austin Corell

Laboratory rodents are typically housed in rooms set at temperatures well below their thermoneutral zone, primarily to match the comfort requirements of the personnel working in these rooms. The rodent thermoneutral zone is the range of temperatures within which rodents maintain their body temperature without expending extra energy to keep warm. Housing rodents in temperatures lower than their thermoneutral zone subjects them to cold stress, which leads to changes in physiology, behavior, and immune function, variably impacting research. The Optimice® SMART system is a compact 20-cage individually ventilated rack with individual heated floorspace provided to 10 cages. Heat levels can be tailored to meet the thermal demands of animals in each cage, and mice can choose the heated floor or a cooler floor surface within the cage. The ability for animals to choose their thermal comfort level sets SMART apart from other systems that either provide heated air to the cage or heat the entire cage bottom area. Participants will learn how SMART can alleviate cold stress in mice and be used to provide thermal support to postoperative mice and other research models. The options to expand the use of the system are vast since SMART is also equipped with 25 watts of USB power ports at each station for connecting additional monitoring, camera, or behavioral devices. The target audience is all lab animal professionals and researchers who are interested in a platform to provide continual thermal support to mice without the limitations of traditional heat support devices.

This Technical Trade Presentation is sponsored in part by Animal Care Systems.

Improving Rodent Breeding Performance with Nutritional Supplementation

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Speaker: Morgane G Stum Moderator: William Thomas

Breeding programs for laboratory research animals are in place in most medical research institutions and can include a variety of genetic strains and backgrounds, from inbreeds to transgenics to newly engineered mice. Common breeding challenges are often observed, including poor breeding performance (nonproductive units, dystocia, small litters), pup health issues (low lactation, mortality, and cannibalism), and weaning complications (low weight, runt, difficulty finding their food and water source). Poor breeding can have disastrous consequences for principal investigators and animal care professionals. On a large scale, poor breeding production can incur increased costs, wasted resources, and additional technician time. On a smaller scale, poor breeding performance can mean delays

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Humanized Mouse Models

Speaker: Jenny Rowe Moderator: TBN

When evaluating the world of humanized models it’s important to consider the unique differences in each model for your researchers’ study needs. Charles River offers multiple options, both founded on the triple-immunodeficient NCG mouse strain. The ability of human immune cells to engraft in the NCG mouse makes it a powerful tool to evaluate compounds for immuno-oncology, infectious diseases, and inflammatory diseases. This presentation will provide an overview of the humanized mouse portfolio, including a summary of models engrafted with either human PBMCs or human umbilical cord blood derived CD34+ stem cells.

This Technical Trade Presentation is sponsored in part by Charles River Laboratories.

Humanized Preclinical Models in Immunotherapy and 3R Guidelines

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Speaker: Erwan Corcuff Moderator: Thomas Tribou

Two levels of preclinical requirements exist in Europe. The first is research without any obligations. The second is research with regulatory requirements. Excluding this differentiation, global preclinical approaches include discussions about the relevance of models to the 3Rs, and how to provide and use more predictive pre-clinical models to meet 3R requirements. Preclinical models have emerged over the past 20 years that can partially reproduce functional human immune systems and associated human tumors, among others. Derived from academic research centers, these specific models make it possible to generate useful information for translational research. In immunotherapy, these models are particularly useful in preclinical trials to demonstrate early rejection or drug candidate inclusions. In parallel with procedure optimizations, the team training and the scientific incrementation of the models has made it possible to reduce the number of animals used.

An effective industrial preclinical base for 10 years, the industrial and scientific development of these advanced models has been justified. The combination of the science, logistics, and production elements makes it possible to meet the 3Rs. This Technical Trade Presentation is sponsored in part by Janvier Labs.

u From Outdated to Cutting-Edge: Transforming a 1970s-Era Facility into a Xenotansplantation Suite Speaker: Alissa McFarland Moderator: TBN

Society’s need to advance research from bench to bedside, coupled with an increasingly competitive life sciences industry, has generated demand for facilities designed to support clinical research and foster the optimization of breakthrough therapies. Translational research using large animal models requires specialized

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

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COVID-19 has changed our attitudes toward infection prevention, both inside the vivarium and throughout our communities. Your expertise in preventing infections in animal housing areas can be a highly effective tool to help protect your team from the threat of infection, in addition to the animals under your care. This presentation will reveal how you can optimize your cleaning and disinfection protocols throughout your facility, with safety to the user and animals at the forefront. The target audience is facility supervisors, vivarium managers, environmental health and safety staff and laboratory animal technicians.

in research results and even loss of unique and precious transgenic lines. A range of precautions can be taken to help at every step of the breeding program to ensure optimum breeding performance. Nutrition is a major influencer to healthy reproduction; however, there is little research on the importance of good nutrition for laboratory animals during this highly demanding process. ClearH2O’s DietGel® Prenatal, a purified dietary gel supplement specifically formulated for breeders contains a variety of nutrients essential to healthy reproduction, pregnancy, and lactation, such as folic acid, iron, calcium and omega-3s. DietGel® Prenatal also contains flax seeds, adding extra foraging opportunity to reduce stress.

Independent and collaborative research studies have shown that using ClearH2O dietary supplement gels to support laboratory animal breeding programs has many benefits, including increased breeding performance, production of larger litters, reduction in cannibalism, and higher survival of pups and weanlings.

Protecting Your Animals and Team with Optimized Cleaning and Disinfection Protocols u

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TECHNICAL TRADE PRESENTATIONS spaces designed to meet multiple criteria at high dollars per square foot. Grant funding often falls short of the capital required for institutions to build new facilities, necessitating programs to grow within existing buildings that lack the infrastructure to support program needs. This situation poses significant design challenges requiring thoughtful programming, planning, and life-cycle cost analysis to implement effective and sustainable solutions that balance user requirements with facility constraints. University of Maryland School of Medicine entrusted CannonDesign to transform existing laboratory space into a state-of-the-art animal surgery suite for cardiac xenotransplantation, where researchers are breaking new ground in the exploration of xenotransplantation to mitigate the shortage of human organs for transplants. We’ll share the methodology and tools used to guide the stakeholders in quick decision making and creative solutions that balance the complex design requirements and challenges presented by the 1978 building, not originally designed to house animals.

This Technical Trade Presentation is sponsored in part by CannonDesign.

Successful Oncology Research Using the 3Rs: Animal Model and Services Selection

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Speaker:Â Sheryl J Wildt Moderator:Â Jamie Naden

The rate of successful translation from animal models to clinical cancer trials is less than eight percent. Proper experimental design may be the difference between the success and failure of an investigational drug. Conducting carefully designed experiments and choosing the right animal and tumor model does not only impact success rate, but also support the 3Rs. Oncology research is heavily dependent on animal work, but here participants will learn the steps for selecting the optimal oncology model for a research study and complementary services to make the most out of each study, each animal, time, and funding. We will discuss various animal models available for oncology research on the market today, including the new B-NDG (NOD.CB17-Prkdcscid IL2rgtm1/Bcgen) ultra-immunodeficient mouse model. In addition, we will discuss the importance of highly characterized tumor models, particularly PDXs, in reducing the number of animals needed to answer research questions. Finally, we will highlight the importance of the sequence and type of studies that are done to collect the maximum amount of data from each animal. The target audience is principal investigators, graduate students, lab managers, technicians, or any lab animal professionals involved in designing experiments for or participating in oncology research.

This Technical Trade Presentation is sponsored in part by Envigo.

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Emerging Animal Rights Trends that Pose a Significant Threat to Biomedical Research and Creating a Roadmap for Response

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Laboratory animal breeders are an essential part of the health research and discovery process. Increasingly, many animal rights groups are replacing traditional protest activities with attempts to introduce legislation at local, state, and federal levels that would ban the breeding, sale, and transport of research animals. Their belief is that if you can eliminate the businesses that produce animals for research, you can ultimately eliminate animal studies completely. The purpose of this session is to alert and educate the research community about this tactic and discuss both current and future strategies to stop harmful legislation that may ultimately end critical, lifesaving research as we know it. This includes advice on what individual members of the audience can do to help defend research. The seminar will bring together experts in government, public, and community relations who effectively deal with anti-research legislation and public relations/outreach. The target audience for this session includes all members of the research community, including those representing organizations with business interests or research requirements that depend on the availability of animal models either directly or indirectly.

Speakers/Topics: Rick VanDomelen

Welcome and Introductions

John J Sancenito

Evolution of Animal Rights Tactics

Rocco Praglowski

Current Legislative Trends Impacting Breeders and the Research Community

Jim Newman

Maintaining Awareness through Public Outreach

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Questions and Answers

Multimodal Analgesia and Anesthesia in Exotic Animals in a Clinical Practice Setting

This Seminar is sponsored in part by American College of Laboratory Animal Medicine (ACLAM) and American Society of Laboratory Animal Practitioners (ASLAP).

Beyond the IACUC: The Current Landscape of Opportunities and Challenges in Research Administration

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Leader/Moderator: Stacy L Pritt Facilitator: TBN

Laboratory animal professionals have long been involved with IACUC management and administration. However, IACUC offices often exist within larger research administration units within institutions. These research administration units typically include other significant components such as human research subjects protection, conflict of interest, export control, stem cell research oversight, grants management, and other federally mandated research oversight activities. For individuals who lead IACUC offices and animal facilities, career advancement often means assuming leadership of other areas of research administration beyond the IACUC. This seminar will explore those career pathways and hot topics in research administration. Building on the experience of several laboratory animal professionals who started with animal facility management or IACUC administration, speakers will highlight the training, skill sets, competencies, and credentials needed to take the next step in a research administration career. Speakers will also highlight how the different areas of research administration that may appear to be separate from animal research are actually interconnected and may become even more so based on evolving government guidelines for the oversight of all research. Laboratory animal professionals, especially veterinarians and individuals in management positions within animal facilities and IACUC offices, will benefit from this discussion.

Specific advice for career advancement will be provided as well as ideas for improving administrative management skills.

Speakers/Topics:

Beyond Buprenorphine: Pain Management in Fish and Exotic Animals

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Stacy L Pritt

Welcome and Introductions

Leader: Heather A Zimmerman Faculty: Cassandra O Cullin Facilitator: Cassandra M Tansey

Stacy L Pritt

Introduction to Careers in Research Administration

Gregory R Reinhard

Leading an IACUC Office

Christopher S King

Oversight of Multiple Research Administration Programs

Stuart E Leland

Hot Topics in Research Administration (Conflict of Interest and Export Control

Deyanira I Santiago

Quality Improvement for Research Administration

Pain management continues to be a challenge within laboratory animal science—from drug class limitations based on research protocols, to disruptions in medication supply chains, to debates on whether or not certain species experience pain. This seminar will provide information on fish analgesia with an emphasis on the zebrafish and multimodal analgesia and anesthesia in exotic animal species with emphasis on rodent species. Multimodal analgesia will be discussed, including alternatives to systemic drugs by using local anesthesia and analgesia. This seminar is targeted towards veterinarians, technicians, researchers, and students and all are invited to listen and interact with seminar speakers in directed question and answer sessions.

Speakers/Topics: Heather A Zimmerman

Welcome and Introductions

Julie A Balko

Recent Advances in Fish Analgesia

Fredric Chatigny

Fish Analgesia: What We Know and Where to Go from There

Building Momentum in Openness about Animal Research in the U.S.

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Speakers: F Claire Hankenson Moderator: Cynthia A Pekow Facilitator: Paula A Clifford

Health discoveries that benefit humans and animals rely on animal research. However, recent public opinion polls show that only half of Americans support health research involving animals. As public support falls, activist groups are successful in placing increased restrictions on research. In response, science organizations are recognizing the need for expanded outreach and communications, a strategy that

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

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Leader/Moderator: Rick Van Domelen Facilitator: Nicole Navratil

Angela M Lennox

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has worked well in other parts of the world to quell public fears and improve trust in science. A growing number of U.S. research organizations, including universities, biotech, and pharmaceutical companies, are posting website information about how and why animals are studied. Participation in public outreach initiatives, such as Biomedical Research Awareness Day, is increasing annually. However, significantly expanded engagement with the public by more institutions is needed to achieve meaningful public discourse about why, when, and how animals are involved in research. Participants will learn why increased openness is essential to scientific progress, the developing U.S. grassroots effort taking place to engage and assist research organizations in becoming open about their work with animals, and the first steps of an Openness Initiative steering committee convened in the U.S. in 2020 to inform a way forward.

Charles River Ethics and Animal Welfare Lecture: Your Perception May Not Be My Reality: How Human Perception Shapes Evaluation of Animal Well-Being During Euthanasia

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Speaker: Debra L Hickman Moderator: Patricia V Turner Facilitator: TBN

Society has granted laboratory animal professionals with the ultimate responsibility of guiding decisions regarding the euthanasia of animals. As compassionate individuals, the profession has generated guidelines and expectations surrounding the decision-making process for this responsibility. Many times, the guidelines and expectations are developed from this place of compassion, with the perspective of the human superimposed on the predicted perspective of the animal. However, the human perspective is greatly shaped by their own experiences and may not be an accurate representation of the actual experience of the animal. An appropriate evaluation of animal well-being requires that the evaluation be at the level of the animal. In this session, we will explore the relationship of veterinary medicine with the range of language associated with the killing of animals and how this language is shaped by human perception. We will also review the tools and traps that are inherent in the evaluation of well-being of animals during these procedures. The ultimate goal of this presentation is to assist the laboratory animal professional in understanding how to objectively evaluate the well-being of individual animals during the euthanasia procedure.

This Special Topic Lecture is sponsored in part by Charles River.

Exploring a New Ethical Framework for the Review of the Proposed Use of Research Animals

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Speaker: Margaret Foster Riley Moderator: Margaret S. Landi Facilitator: B Taylor Bennett

In their recently published Principles of Animal Research Ethics, eminent philosophers Tom Beauchamp and David DeGrazia offer six principles that they claim offer a framework for the evaluation of animal research that can be accepted by both proponents and opponents of animal research. The framework is the first major challenge to the 3Rs rubric that has dominated evaluation of animal research over the last fifty years. Arguably, the framework is not a challenge but an overlay; one that promises to enrich the 3Rs analysis. To help understand both the promise and potential weaknesses of these principles, we will consider several case studies and explore the implications of the Beauchamp-DeGrazia framework and how it might work within current regulations. The target audience will be all those involved with the preparation and review of proposals to use animals in research.

This Special Topic Lecture is sponsored in part by Scientist Center for Animal Welfare (SCAW).

Harnessing the Media to Enhance Public Understanding of Animal Research q

Leader: B Taylor Bennett Moderator: Matthew R Bailey Facilitator: TBN Panelists: Eva C Maciejewski, Kirk Leech

Traditional and social media are increasingly being used by animal rights activists to target public and private institutions that conduct animal research. While a negative news story is not the same as an act of vandalism or a protest, the consequences are no less devastating for research organizations. In this session, the panelists will discuss the use of media, both traditional and social, to counter anti-animal research propaganda and to explain the purpose of animal research.

The tools and guidelines that have been effective in North America and Europe to harness the power of the media both in crisis situations and in non-crisis situations will be reviewed. The panelists will highlight their own experiences with crisis communications and lessons they have learned as means of encouraging members of the audience to discuss their own media experiences. The target audience will be all those who are interested in using today’s media to enhance the public’s understanding of animal research.

This Panel Discussion is sponsored in part by Foundation for Biomedical Research

Increasing Openness and Transparency in Animal Research: Institutional, Personal, and Innovative Approaches from around the World

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Leader: Jodi Salinsky, Kirk Leech Moderator: Patrick E Sharp Facilitator: TBN

Since the spring of 2018 the European Animal Research Association (EARA) has been assessing the websites of European institutions that carry out biomedical research using animals. The results, from close to 1500 websites assessed, reveal that while many institutions have moved towards a more transparent approach, in many countries the research community is still reluctant to provide suitable information for the public on the research it conducts. In the digital world, communicating to the public on the importance of animal research via an institution’s website is an important way of increasing understanding and awareness of the work of the life sciences sector. This openness allows researchers to place animal research, in context, as a necessary part of biomedical research with benefits to both humans and animals. In this seminar, we will evaluate the EARA website survey and provide examples of institutions who are leading the way in seeking to improve institutional transparency with the public. We will hear about methods of personal and institutional openness in various countries and locations and look at how to establish good practice for a long-term communications strategy. This includes a 12-step plan that provides institutions with a practical tool to establish a robust and effective communications platform on animal research and enable them to handle communications in crisis situations. Target audience is anyone actively or peripherally working in laboratory animal science or any aspect of the animal research environment, such as animal technicians, veterinarians, facility managers, industry representatives, and organizational leadership.

Speakers/Topics: Patrick E Sharp

Welcome and Introductions

Kirk Leech

The Zeitgeist of Openness in Animal Research

Kirk Leech & Jodi Salinsky

What Are the Barriers towards Greater Openness

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welfare , regulatory compliance , and public education Twelve-step Plan to Establish a Robust and Effective Institutional Communications Platform

Kirk Leech & Jodi Salinsky

Questions and Answers

Ongoing Changes within the Animal Research Oversight Environment

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Leader/Moderator: B Taylor Bennett Facilitator: Rocco Praglowski

Measuring the Success of Outreach Efforts: Does Public Polling Tell the Full Story and How Do We Truly Gauge if We Are Moving the Needle?

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Leader/Moderator: Paula A Clifford Facilitator: Jim Newman Panelists: Wendy Jarrett, Jodi Salinsky, Eric P Sandgren

According to public polling data, approximately 50% of Americans are opposed to the use of animals in biomedical research. In response to this alarming statistic, both public and private research organizations across the United States are increasing outreach and public communication efforts. In addition, universities, research organizations, and pharmaceutical companies have launched an effort to form a transparency agreement in the United States. However, while public engagement is increasing, research organizations are struggling to measure the impacts of their outreach efforts. The availability of good data to gauge effectiveness is critically important for outreach planning purposes and for maintaining institutional support for advocacy work. In other countries, such as the United Kingdom, in depth public polling data, which includes a variety of questions about animal studies, help the research community better understand the impacts of their education efforts. In comparison, polling in the United States and New Zealand/Australia is minimal. Surveys that address this issue are often limited to one or two general questions. Another common concern among research organizations is whether public polling alone provides enough meaningful data to guide effective outreach efforts. This session will focus on currently available systems for measuring communications effectiveness. Presenters will propose additional, unique strategies that can be used for gauging the success of individual outreach programs and whether current animal research communication strategies are effective for public audiences. The presentation will also identify areas where the United States and other countries might seek to increase the availability of metrics to gauge overall public attitudes about the role of animal studies in biomedical research.

This Panel Discussion is sponsored in part by Americans for Medical Progress (AMP) and Understanding Animal Research.

DEB HICKMAN

On August 28, 2019, the National Institutes of Health released the Final Report on Recommendations To Reduce Administrative Burden on Researchers in response to the mandate in Section 2034 of the 21st Century Cures Act (Cures). The report contained actions proposed by the working group from the NIH, USDA, and FDA to reduce administrative burden on investigators while maintaining the integrity and credibility of research findings and protection of research animals. In addition, the USDA has been reviewing the Animal Care Policy Manual and the Animal Welfare Inspection Guide. The changes that will occur as result of these activities will have an impact on how animal care and use programs are managed. This seminar will provide the attendees with an opportunity to hear from representatives of the USDA, OLAW, AAALAC, the DOD, and NABR regarding possible changes, as well as other ongoing issues within their organization and to discuss with those representatives how their organization’s activities impact the environment in which we work and what changes to expect in the future. Questions for the panelists can be submitted to btbdvm@yahoo.com. The target audience will be those who need to keep current with the regulations and requirements for conducting animal-based biomedical research.

Speakers/Topics: B Taylor Bennett

Welcome and Introductions

B Taylor Bennett

Discussion

Elizabeth Goldentyer

USDA Update

Patricia Brown

OLAW Update

Kathryn Bayne

AAALAC Update

Dawn C Fitzhugh

DOD Update

Matthew R Bailey

NABR Update

This Seminar is sponsored in part by National Association for Biomedical Research (NABR), United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Animal Care, National Institutes of Health (NIH) Office of Laboratory Animal Welfare (OLAW).

CHARLES RIVER LECTURE

Dr. Hickman is the Director of the Laboratory Animal Resource Center at Indiana University School of Medicine in Indianapolis, IN. She received her veterinary degree from University of Illinois, Urbana-Champaign, where she also completed a residency in laboratory animal medicine. Her professional expertise spans across academia, government, and commercial research programs. Her research interest is behavioral and physiological assessment of animal welfare with a special emphasis on euthanasia, biomethodology, and husbandry. Dr. Hickman holds a master’s degree in veterinary clinical medicine and is board certified in laboratory animal medicine and animal welfare. She currently serves as a member of AAALAC International Council on Accreditation and is the Vice President of the American College of Laboratory Animal Medicine. qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

Kirk Leech & Jodi Salinsky

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26 A nimal

welfare , regulatory compliance , and public education

On the CUSP: A New Option for Addressing Administrative Burden

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Under the auspices of the Federal Demonstration Partnership (FDP), the Compliance Unit Standard Procedure (CUSP) Project offers an option to address administrative burden at the institutional level and improve reproducibility within and across institutions. The goal of this project is to create an online repository where institutions can share standard procedures used in animal care protocols with the broader animal welfare compliance community. Use of this tool is supported by OLAW and the USDA and is a burden-reducing initiative from the 21st Century Cure Act. A working group, representing over 40 institutions, has been formed to support site design and development. The group has made significant progress in developing this resource over the past year. This session will provide participants with an overview of the project, including responsibilities of participants using the system, a demonstration of the test site, and an update on the status of the project. The targeted audience is IACUC members, veterinarians, postapproval monitors, and researchers.

What basic tools are required to start a podcast? What are the advantages and hurdles of using podcasting? How can podcasting help communicate difficult topics on human and animal health to the public? How can podcasting pivot the discussion to better engage public opinion? What unique aspects of podcasting help tell facts and not fiction? How might a podcaster or public relations strategist improve upon their storytelling skills to execute a heartfelt message that speaks to society with compassion and transparency? The audience participants will learn essential resources needed to launch a podcast, insights into the art and goal of storytelling, and the use of podcasting as a platform for public relations, marketing, and messaging of your mission. In the world of pandemic paranoia and misuse of media, getting in front of the story through podcasting allows for a more balanced log of oral history passed from generation to generation. Podcasting and other digital media platforms now trending provide a unique opportunity to educate the public on matters of animal science without a face-to-face conversation. Panelists will reflect on their podcast experiences and provide audience members insights, tools, and resources. Audience members may be interested in launching a podcast, managing a podcast for their organization, learning how to scale their podcast audience, or gaining insight into the power of podcasting for use in public relations.

AAL AS 71 st N AT I ON AL MEET I N G

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Leader/Moderator: Sally Thompson-Iritani Facilitator: Jennifer N Davis

Speakers/Topics:

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Podcasting to the Public and the Art of Storytelling

Leaders: Angela G Stoyanovitch Moderator: Cindy A Buckmaster Facilitator: Alice White McVey Panelists: Angela G Stoyanovitch, Tom Leach, Jeff Marshall, Megan R LaFollette

Sally Thompson-Iritani

Welcome and Introductions

Elaine K Kim

Using CUSP to Decrease Burden and Maintain Compliance

This Panel Discussion is sponsored in part by Legal Drugs Agency and Americans for Medical Progress (AMP).

Madeline L Budda

Tailoring CUSP to Your Institution (Small, Large, Academic, Industry)

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Scott D Bury

Convincing the IACUC and Leadership that CUSP is Right for You

Anthony E Munn

Transitions and Change Management with CUSP in Mind

Sally Thompson-Iritani

Live Demo of the CUSP Site, Q&A

Organ-On-Chip Technologies: Hype or Reality?

Speaker: Szczepan W Baran Moderator: Marcel I Perret-Gentil Facilitator: TBN

Organ-on-a-chip (OOC) technology refers to a miniature device that recapitulates an organ’s structure and its function in vitro. This technology has a potential to impact various stages of drug discovery and development including efficacy and safety, with the additional potential benefit of impacting the 3Rs in preclinical studies. There is a significant amount of hype regarding this technology that exceeds what is currently technically feasible. While there has been an enormous amount of progress in this field, the technology is still in its infancy and requires characterization, validation, and optimization before we can fully understand its potential. This lecture will provide an overview of the current state, challenges that remain before these technologies can be fully incorporated into drug discovery and development, and realistic possibilities of organ-on-a-chip technology.

Reaching the Next Generation through Innovative Ways to Connect with Students Both Inside and Outside of the Classroom

Leader: Logan K France Moderator: Paula A Clifford Facilitator: TBN

Medical progress requires a significant amount of public trust in science and research. Effective public education about biomedical research, including essential animal studies, requires many different approaches. One method involves engaging with future generations, namely students. There are several effective programs and opportunities available for advocates interested in inspiring and educating students of all ages. These range from classroom visits to volunteering at science festivals or supporting summertime science camps. Activities, lesson plans, and other resources are available for all levels of students. This session will highlight specific programs that provide ample resources or opportunities to interact with students, while educating them about biomedical research. Information about out how to access or implement these programs will also be included. Attendees will also learn effective strategies for engaging different levels of students from elementary, middle school, high school, and college undergraduate. Participants will learn the importance of teaching students about animal research; effective strategies for engaging different levels of students from elementary, middles school, high school, and undergraduates; specific programs that provide ample resources or opportunities to educate students about biomedical research and the role of animals; information about how to access or implement the programs presented; and resources available to assist in reaching out to and interacting with students.

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

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welfare , regulatory compliance , and public education Speakers/Topics: Welcome and Introductions

Wendy Jarrett

Understanding Animal Research’s Animal Research Conversations

Vicki C Campbell

AALAS Foundation’s K-12 Classroom Resources

Tom Leach

SPARC and other Informal Animal Research Education Resources

Ken Gordon

SPARC and other Informal Animal Research Education Resources

This Seminar is sponsored in part by American College of Laboratory Animal Medicine (ACLAM) and American Society of Laboratory Animal Practitioners (ASLAP).

Research Animal Behavioral Management for the 21st Century

Leader/Moderator: Patricia V Turner Facilitator: TBN

Improving the welfare of research animals and increasing the validity and reproducibility of scientific outputs attained from working with animals in science in the 21st century requires taking the next step and developing an approach that not only addresses reducing potential pain and distress, but also promotes positive welfare and experiences. This can be achieved by implementing a comprehensive behavioral management program for all species that we work with and care for in research facilities. This seminar will discuss what a comprehensive behavioral management program should address for research animals and how to move our institutions in this direction, as well as provide concrete examples of how this is being addressed for a range of common research animal species. After attending this session, participants will be able to describe necessary components of behavioral management programs that should be considered for all species, how to better prepare research animals for scientific procedures, and how addressing behavioral management improves overall animal welfare, scientific outcomes, and employee satisfaction. This seminar is intended for animal caregivers, research and behavior personnel, veterinary professionals, IACUC members, and scientists.

Speakers/Topics: Patricia V Turner

Welcome and Introductions

Patricia V Turner

An Introduction to Components of a Comprehensive Research Animal Behavioral Management Program

Peter Fisher

Training Dogs in a Research Environment

Fernando De La Garza

Low-stress Handling and Behavior Management Techniques for Pigs

Aileen Miline

Improving Macaque Behavioral Management to Achieve More Consistent Scientific Outcomes

Mark J Prescott

Looking Ahead: Results of an International Survey on Nonhuman Primate Behavioral Management

This Seminar is sponsored in part by Charles River Laboratories, The National Centre for the Replacement, Refinement and Reduction of Animals (NC3Rs) and GlaxoSmithKline (GSK). q

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A New Approach to Working with Rats in Research

The Future of the Guide

Leaders: Margaret S Landi, Robert C Dysko Moderator: Margaret S Landi Facilitator: Bruce W Kennedy Panelists: Caroline J Zeiss, Anne Maglia, Robert C Dysko, Steve M Niemi

It has been almost 10 years since the 8th edition of the Guide for the Care and Use of Laboratory Animals was published by the National Research Council. Based on subsequent advances in laboratory animal science, and recent legislative and animal use discussion, there is a need to update the Guide and consider expanding it to include other disciplines of animal research. This moderated listening and audience participation session is sponsored by the Institute of Laboratory Animal Research (ILAR), part of the National Academies of Science, Engineering and Medicine. The session will revolve around the following questions. If the Guide was to be updated in phased approaches, what areas should be prioritized? Should any revisions go beyond laboratory animals and include agricultural animals and wildlife/field work? What about basic and applied research using pets? Should professional judgement, performance standards, and harmonized approaches be replaced by more engineering standards? Should a new format for the Guide be considered? Classic hardback or more of a Wikipedia model?

This Panel Discussion is sponsored in part by Institute for Laboratory Animal Research (ILAR).

(FBR) and European Animal Research Association (EARA).

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

Logan K France

Marie Eriksson

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Accelerating Drug Discovery with Non-Invasive Animal Imaging: The 3Rs Perspective AAL AS 71 st N AT I ON AL MEET I N G

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Leader: Dinesh K Hirenallur-Shanthappa Moderator: Terri A Swanson Facilitator: Judith E Fenyk-Melody

Responsible lab animal medicine is undertaken with the 3Rs in mind. Efforts are always made with the goal of reducing the number of subject animals needed for a study, as well as performing techniques designed to minimize pain and stress on our subjects. Noninvasive imaging modalities are the perfect way to obtain biological information in living subjects without significantly disrupting their normal physiology. In the field of drug discovery, imaging modalities that are translatable, meaning capable of being used on animals and humans, are the most desirable as they provide comparable information on the efficacy of candidate compounds. This seminar will focus on translatable imaging modalities and how they are helping scientists make sound decisions on which drugs are suitable to go to clinic while also adhering to the utmost in animal welfare. This seminar is targeted toward all animal technicians and scientists who may be interested in the scientific impact of the work they do every day. Attendees will learn about several in vivo imaging techniques. Each talk will cover an individual imaging technique, describing the basic science behind the technology, how it is used in drug discovery, and how each technique can translate into the human clinic. Specific instrumentation that will be covered includes micro CT, ultrasound echocardiography and elastography, and PET. Emphasis will also be made on how the technique contributes to animal welfare, tying scientific discovery to animal care and how the two go hand-in-hand in pushing life-saving drugs into the hands of patients who need them.

Speakers/Topics:

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Terri A Swanson

Welcome and Introductions

Jeffrey P Morin

Advancement in Ultrasound Applications in Preclinical Drug Discovery

Dinesh K HirenallurShathappa

Advancement in Ultrasound Applications in Preclinical Drug Discovery

James Goodman

Translational MRI in Drug Discovery and Development

John M David

PET Imaging in Preclinical Drug Discovery

Crystal Bluette

Application of micro CT Imaging to Assess Body Composition in Preclinical Animal Models of Cachexia

Best Practices for Inflammation-based Animal Models

Leader: Portia S Allen Moderator: A Marissa Wolfe Facilitator: Ania Skorupski

In the decade following publication of the Animal Research: Reporting of in Vivo Experiments (ARRIVE) guidelines, laboratory animal professionals and the scientific community alike have placed emphasis on the standardization and refinement of animal models of human disease. Core scientific organizations are collaborating at national and international levels to develop position statements, policies, recommendations, and/or best practice guidance on the conduct of animal research in their respective fields of study. Animal studies involving the induction of inflammation have been particularly challenging to address as confounding variable concerns are frequently raised, from model induction to refinement strategies. The

goal of this seminar is to connect the laboratory animal care community with published, field-driven best practice guidance for inflammation-based animal models: sepsis, venous thrombosis, stroke, and obesity. Attendees will learn the current state of animal model recommendations, with an emphasis on rodent models, put forward by these field experts. Awareness of these standards will assist laboratory animal professionals in the provision of high-quality animal husbandry and veterinary care and promote understanding and refinement of procedures proposed in animal use protocols. This session will be of interest to individuals involved in the conduction, care, and regulatory oversight of these animal models, including vivarium managers, veterinary staff, research scientists, and IACUC members or administrators.

Speakers/Topics: Portia S Allen

Welcome and Introductions

Jean A NemzekHamlin

Best Practice for Sepsis Models: The MOTiPSS Guidelines

José A Diaz

Best Practice for Venous Thrombosis Models

Mark J Prescott

Best Practice for Stroke Models: The IMPROVE Guidelines

Portia S Allen

Best Practice for Obesity Research

This Seminar is sponsored in part by American College of Laboratory Animal Medicine (ACLAM) and American Society of Laboratory Animal Practitioners (ASLAP). v Biological Materials Testing: An Important Component of an Animal Resources Biosecurity Program Leaders: Cindy L Besch-Williford, Robert S Livingston Moderator: Cindy L Besch-Williford Facilitator: Jeffrey Kovacs

Transplanted cells and tissues engrafted in immunocompromised rodents provide a valuable preclinical model system to study a wide variety of human diseases and evaluate responses to new therapeutics. Model development involves use of various human- or animal-derived biological materials, such as patient-derived xenografts, human blood and other tissues, and cell lines for tumor formation. It has been documented in scientific literature that these research reagents can become contaminated with infectious agents or with cells from another species or cell type.

The possible negative outcomes of using contaminated biological materials include infectious disease outbreaks if the biological material contains rodent pathogens, risk of exposure of laboratory and vivarium personnel to human infectious agents if the biological material is of human origin, and the generation of unusable data if the biological material was misidentified or contaminated with other cells. This seminar will overview the contribution of contaminated biological materials to the reproducibility crisis in preclinical research, provide examples of biological material contamination such as with Corynebacterium bovis and Mouse kidney parvovirus, and screening methods that can be instituted as part of a comprehensive biosecurity program. The targeted audience is laboratory animal veterinarians, facility managers, and animal welfare policy and compliance personnel.

Speakers/Topics: Cynthia L BeschWilliford

Welcome and Introductions

Robert Livingston

Viruses in Human-Sourced Biological Materials

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

B iomedical

research , medicine , and methodology

Bacterial and Fungal Contaminants of Cells and Cell

Cynthia L BeschWilliford

Misidentification and Genetic Contamination of Cell Lines and Xenografts

Robert Livingston

Summary and Questions

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v Charles C Hunter Lecture: Rational Design of Novel Treatments for Psychiatric Illness Speaker: Flavio Frohlich Moderator: Julie A Avalos Facilitator: TBN

Dr. Frohlich will show how a biomedical discovery made under the microscope in a lab dish has enabled the development of a novel, noninvasive brain stimulation paradigm. His lab has discovered that electrical communication signals in the brain are surprisingly susceptible to weak electricity, which can be used to sculpt and restore neuronal communication. Today, this noninvasive approach is being investigated in clinical trials for psychiatric and neurological illnesses such as depression, schizophrenia, and chronic pain. This successful translation was enabled by preclinical biomedical research using a ferret model, which provides the basis for the rational design of personalized treatment paradigms by allowing insights into the mechanism of action of brain stimulation. He will discuss how combining computer simulations, preclinical research, and clinical trials provide a novel approach that leverages synergy and thereby accelerates the journey towards greatly improving animal and human brain health. Participants will learn the unique and important role of species other than mice and rats in brain science, the role of brain rhythms in health and disease key considerations for rapid translation from basic science to clinical trials, brain networks as novel treatment targets of psychiatric and neurological illnesses, and a new perspective on biology that focuses on networks and ecosystems rather than organs and individuals. The target audience is laboratory animal technicians, veterinary technicians, veterinarians, and research personnel.

This Special Topic Lecture is sponsored in part by Committee for Technician Awareness and Development (CTAD).

FLAVIO FROHLICH

Creation, Care and Utility of Humanized Mouse Models

Speaker: Jenny Rowe Moderator: Kenneth S Henderson Facilitator: Kourtney P Nickerson

Immunodeficient mouse models have been widely used to study human diseases over the past few decades. An emerging trend in immunodeficient models is the increasing use of specialized models engrafted with human immune systems, or “humanized mouse models.” Humanized mouse models are powerful tools for evaluating preclinical applications, including the safety and efficacy of immune checkpoint inhibitors and other compounds that function by modulating human immune cell components. Humanized mice have unique advantages in providing a more accurate preclinical model for the evaluation of immunooncology therapeutics. This session will provide an overview of humanized mouse models, including technical procedures to humanize immunodeficient mice and health monitoring guidelines. We will also discuss the immunophenotypic characterization and performance of a new humanized mouse model and demonstrate application in immunooncology. This lecture will be of considerable interest to many meeting attendees, including lab animal scientists, laboratory animal medicine specialists, veterinarians, research scientist, technicians, and select vendors. v Grants for Laboratory Animal Science: Do You Want $50K for Your Research?

Leader/Moderator: Victoria K Baxter Facilitator: TBN Panelists: Robert T Dauchy, Nicole E Duffee, William W King, Kelly A Metcalf Pate

Interested in submitting a Grants for Laboratory Animal Science (GLAS) application but don’t know where to start? Members of the AALAS Scientific Advisory Committee (SAC) and previous successful GLAS awardees will discuss the benefits of funding research through the GLAS program and give the audience tips on how to take their idea and translate it into a successful GLAS application. The GLAS mission is to enhance scientific knowledge in laboratory animal science through research.

Examples of research topics previously awarded through the GLAS program include extrinsic environmental conditions, housing and enrichment, pain and distress, health and welfare, euthanasia, and advancements in animal care and use. The panelists will discuss the types of awards available through the GLAS program; how

HUNTER LECTURE

Dr. Frohlich is a member of the Comparative Medicine Institute at North Carolina State University in Raleigh, NC. He also serves as an Associate Professor in the Department of Electrical and Computer Engineering at NC State and an Associate Professor in the Department of Psychiatry at the University of North Carolina in Chapel Hill, NC. He is the founder and Chief Scientific Officer of Pulvinar Neuro, LLC., and is a Director at the Carolina Center for Neurostimulation. Dr. Frohlich received his PhD in Computational Neurobiology at the University of California, San Diego. He has an International Diploma from the Imperial College in London, UK, in electrical and electronic engineering.

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

Marcus Crim

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to craft a successful application, including establishing a hypothesis, designing experiments with appropriate controls, outlining clear concise methodologies, choosing proper statistical analyses, and justifying various components of the budget; and common application pitfalls to avoid. The panelists consist of current and former SAC chairs, researchers who have recently received GLAS awards, and an administrator for the GLAS program. The target audience will consist of individuals interested in obtaining extramural funding for a research project at their institution, and attendees will attain invaluable insight the GLAS program and application process. The session will build on previous years’ presentations providing details regarding the types of grants available through the GLAS program and outlining the components of a successful GLAS application. v How to Start, Maintain, and Expand the Capacity of a Germ-Free/Gnotobiotic Program: A Review of Planning, SOPs, Training, New Equipment, and Resources Proven to be Successful Leader/Moderator: Brian Bilecki Facilitator: Joe Romano

As the demand for microbiome research increases dramatically, many facilities are either implementing expansions into germ-free and gnotobiotic research, growing their existing colonies and capabilities, or even creating a new dedicated unit.

These novel adaptations require new equipment, procedures, supplies, SOPs, and sometimes new competencies, all of which may be significantly different from traditional research strategies and operations. Leading facilities aim at achieving their goals of colony establishment, breeding and research procedures through rigorous and consistent bio-exclusion practices, i.e. “managing sterility,” while maximizing the overall research operational efficiency (housing capacity, set-up times, versatility, personnel, cost, etc.) within their program. A case study of two of these leading facilities will allow insights into their integration of newer technologies, methods, and strategies that motivated change and growth in diverse programs.

Also covered will be the global perspective and key considerations on implementation of the germ-free and gnotobiotic research, and how European facilities have implemented similar initiatives and a review of their practices and results.

Participants will learn about the main drivers for gnotobiotic colony initiation and growth, the type, and volume of research being achieved, the origination of rodent colonies, the use of new equipment and SOPs required for successful containment, and strategies and implementation for program growth and sustainability. The targeted audience for this seminar is facility directors, supervisors, and senior and junior technicians.

Speakers/Topics: Brian Bilecki

Welcome and Introductions

Brian Bilecki

The State of an Increasing Demand for Microbiome Research Facilities

Joe Romano

Facilitator

Dr. Felix R Wolf

Expansion of a Gnotobiotic Research Program at an Academic Institution Using Individually Ventilated Isolation Cages

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Paula L Roesch

Validating the Use of Sealed Positive Pressure (SPP) Housing and Defining Processes between New Housing and Traditional Isolators

Patrick Hardy

Global Perspective and Key Implementation Considerations for Germ-Free and Gnotobiotic Research

Brian Bilecki

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Nathan E. Brewer Lecture

Speaker: Robert H Weichbrod Moderator: Jane M Olin Facilitator: TBN

This Special Topic Lecture is sponsored in part by AALAS Awards Selection Committee (ASC). v

Pathology Quiz Bowl

Leader: Cynthia L Besch-Williford, Craig L Franklin Moderator: Craig L Franklin Facilitator: Crysti Reed Panelists: Craig L Franklin, Cynthia L Besch-Williford

This session will consist of an informal review of the pathology of laboratory animals in the form of an image-based quiz. Topics will include lesions of well-described infectious and noninfectious diseases, pathological manifestations of emerging diseases, and selected phenotypic characteristics of important genetically engineered animal models. The images will be educational and challenging to laboratory animal specialists at all levels of pathology expertise. Targeted audience is comparative medicine trainees, laboratory animal veterinarians, pathologists, and scientists. Participants from comparative medicine training programs can receive a fabulous cash prize for the highest score. A participation cash prize is also provided. The comparative medicine trainee with the highest score will be recognized at the Committee for Laboratory Animal Training and Research (CLATR) luncheon on Tuesday.

This Panel Discussion is sponsored in part by Committee for Laboratory Animal Training and Research (CLATR) and IDEXX BioAnalytics.

Reimaging Preclinical Studies through Digital Transformation: Leveraging Computer Vision, Machine Learning, Mixed Reality, and Informatics Platforms to Maximize Data Quality and Clinical Relevance of Preclinical Studies v

Speaker: Szczepan W Baran Moderator: Marcel I Perret-Gentil Facilitator: TBN

As new technologies emerge, it is imperative to keep current so that we are able to leverage them for animal wellbeing and scientific impact of animal studies.

Computer vision and machine learning allow for continuous and noninvasive animal monitoring for changes in behavior, physiology, and environment. These monitoring technologies provide an opportunity to optimize preclinical toxicology and efficacy studies by collecting physiologically and translationally relevant data, assessing disease and safety states more objectively with quantitative measures. Tele-clinical assessment allows animal technicians to connect with veterinarians and scientists in real-time and expedite decisions. Integration of these technologies

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

B iomedical

research , medicine , and methodology

v Risk Assessment and Decontamination in Laboratory Animal Research Speaker: Jason S Villano Moderator: Lisa Steiner Facilitator: Janet Follo

The use of animals in research comes with the innate risk of accidental exposure of personnel to various hazards, including chemical agents and infectious materials.

This lecture will focus on two important aspects of managing risks associated with the use of these hazards: 1) risk assessment, which involves identification of the risks for specific personnel in a given situation and environment, and 2) decontamination, which renders an area or material safe to handle and reasonably free from a risk of disease transmission. Basic principles and updates from the revised Biosafey in Microbiological and Biomedical Laboratories (BMBL) will be presented and a systematic approach for risk assessment and decontamination using the 4Ws (what, who, when, and where) and 1H (how) will be introduced, especially citing real-life scenarios. The audience will gain fundamental understanding and new paradigms of safe use of hazards in animal research. Target audience include those in animal care and use program management, veterinarians, and IACUC office, EHS, and husbandry personnel.

disease condition. In many instances, immunotherapy has led to new therapies for diseases that have been untreatable in the past, including autoimmune disease and cancer. Basic and translational research in animal models have been critical to the development of these therapies by advancing our understanding of the immune system in various diseases. Immunotherapy offers the unique ability to regulate the immune system by either increasing or decreasing its function based on how the immune system contributes to the disease outcome. For example, immunotherapy is being used to inhibit inappropriate immune responses that lead to autoimmune diseases and transplant rejection. By contrast, immunotherapy is also being used to augment immune responses that are inhibited in cancer and chronic viral disease. Despite these advances, some diseases are resistant to immunotherapy and could benefit from the development of new immunotherapies. Animal models continue to play an essential role in understanding these mechanisms of resistance, thus guiding how the next generation of immunotherapies are being developed. Audience members will first learn about what immunotherapy is and how animal models have been critical to its development. Speakers will then discuss how small and large animal models are impacting the development of cutting-edge immunotherapy in the treatment of viral diseases including HIV, transplantation, and oncology. This seminar series will be of interest to a broad range of audience members interested in how animal models are used to advance human and animal health.

Speakers/Topics:

The Intricacies of Neoplasia and Its Therapeutics in Zoological and Exotic Animals v

Speaker: Tara M Harrison Moderator: TBN Facilitator: TBN

Neoplasia has been diagnosed regularly in multiple zoological and exotic animals.

Through the acceptance of routine physical examinations neoplasia is being diagnosed at a time that treatments are possible. Many neoplasms in exotics are well described, such as lymphoma in ferrets, but what about lymphoma in a guinea pig, a lion, or leukemia in a bearded dragon? How do you calculate medications or even decide what to prescribe? How common are these neoplasms? How do these neoplasias act similarly or differently to other animals and are there any cancer protective mechanisms that these species possess or not? The exotic species cancer research alliance (www.escra.org) along with Arizona Cancer and Evolutionary Center is working on these problems. A centralized database on cancer and its treatments across species is being compiled to answer and provide insight to clinicians faced with diagnosing cancer in their patients. This presentation will not only introduce common cancers that are being diagnosed in a variety of zoological and exotic animals, but will also introduce therapies that are having success as well as preliminary research analyzing survival of these patients with neoplasia.

This Special Topic Lecture is sponsored in part by American College of Laboratory Animal Medicine (ACLAM) American Society of Laboratory Animal Practitioners (ASLAP) Program Committee.

The Role of Animal Models in the Immunotherapy Revolution v

Leader: Zachary T Freeman Moderator: Courtney Hunter Facilitator: Kelly A Metcalf Pate

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Zachary T Freeman

Welcome and Introductions

Courtney Hunter

Introduction to Immunotherapy

Victoria Baxter

Immunotherapy in Rodent Models: Approaches and Considerations

Diana G Scorpio

Immunotherapy Development in Large Animal Models of HIV and Other Infectious Diseases

Rimon Duran-Struuck

Immunotherapy in Large Animal Models of Transplantation

Zachary T Freeman

Reverse Translation: Utilizing Patient Data to Improve the Use of Mouse Models for the Development of Cancer Immunotherapy

Wallace P. Rowe Lecture

Speaker: Sue VandeWoude Moderator: Lon V Kendall Facilitator: TBN

Domestic and nondomestic cats are hosts to a number of retroviral infections that result in lifelong disease but have significantly different disease outcomes.

Studies of these viruses in both natural and experimental infections have provided insights into retroviral pathogenesis, host immune defenses against these unique agents, and viral mutations that result in adaptation to primary and spillover hosts.

These studies have implications for understanding basic retroviral biology in many species, including humans. This session will focus on feline immunodeficiency virus (FIV), Feline Foamy Virus (FFV), and Feline Leukemia Virus (FeLV) infections of domestic cats, and our emerging understanding of these agents stemming from application of modern molecular analyses.

This Special Topic Lecture is sponsored in part by Committee for Laboratory Animal Training and Research (CLATR).

Immunotherapy has transformed human and animal medicine by offering therapies that can modulate the existing immune response to impact the course of disease.

Immunotherapy is any therapy that acts to modulate the immune system to treat a

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

into informatics platform makes animal data accessible, retrievable, learnable, and increases efficiencies of daily processes. This lecture will describe how engagement with these technologies enhances clinical assessments and training and results in real-time actionable insight, faster decisions, reduced biocontamination and noncompliance risks, and increases harmonization and clinical relevance.

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Exhaust Air Dust PCR Update: A Pathogen Screening Method for Many Vivariums, but Not for All AAL AS 71 st N AT I ON AL MEET I N G

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Leader/Moderator: Kenneth S Henderson Facilitator: Stefanie L Deluca

Ten years ago, soiled bedding sentinels (SBS) were the primary screening method for routine pathogen screening. It has been documented that many agents are not easily transmitted to or detected via SBS. Because of this revelation, other screening methods such as direct sampling of resident research rodents for PCR and whole blood microsampling for serology have improved detection of many agents. Exhaust air dust (EAD) collection for pathogen nucleic acid detection by PCR opened another possibility for screening. It was demonstrated that the air movement on IVC racks facilitated the collection of EAD from cages or entire rack systems depending if the IVC had cage-level filtration or unobstructed air from all cages moving to a common collection point. EAD PCR methods improved pathogen detection (refine) and reduced or eliminated the need for SBS (reduce and replace) supporting the 3R philosophy. This session will focus on the use of IVC racks with unobstructed EAD collection that facilitates multiple collection schemes for the entire rack, thus effectively replacing the need for SBS. Experimental and user data demonstrating the efficacy of detecting pathogens by EAD and financial advantages will be presented. As confirmation strategies for unexpected EAD findings are not always apparent, this session will review recommendations for different pathogen groups. With the understanding that not all housing systems can accommodate EAD, the use of SBS may still need to be an important element of a pathogen screening program and will be addressed. This session is intended for animal technicians, facility managers, and veterinarians who play a role in designing and managing health monitoring programs.

Speakers/Topics: Kenneth S Henderson

Welcome and Introductions

Cheryl L Perkins

Show Me the Data! Investigate EAD Studies and the Lessons from the Diagnostic Laboratory

Kerith R Luchins

Is the Juice Worth the Squeeze?

Diagnostic Outcomes and Cost Efficiencies

Kenneth S Henderson

Pathogen Safari: Hunting Down the Source of a Positive EAD Finding on Your IVC

Rajeev K Dhawan

Is There Still a Place for Soiled Bedding Sentinels?

Overcoming Interruptions to Daily Operations in a Large Rodent Vivarium u

Leader/Moderator: Andrew T Pesek Jr Facilitator: Jessica L Grahmann

This seminar will cover UT MD Anderson Cancer Center’s planning, actions, and response to recent events that disrupted major business functions of the Houston animal care operations. The events included a major cagewash renovation that originated as a simple cage washer replacement project but grew in both scope and duration. Additionally, we managed a planned weeklong animal drinking water outage, necessitated by the replacement of rodent drinking water supply lines, that required us to support the hydration of thousands of mice without routine drinking water. We experienced a massive, unexpected citywide water outage that arose due to the rupture of a 96-inch water line, causing a complete loss of water to half of the city, including the Houston Medical Center and our animal facilities.

Lastly, as is the case for every institution in the country, we grappled with the COVID-19 crisis, which remains a chronic challenge to be managed. Each of these disruptions had different and variable impacts on operations, personnel, research,

and the animal population. For expected events, advance planning successfully mitigated the potential impact, allowing for the reallocation of resources and effort. Business continuity planning can address unplanned events; however, some events are particularly unique. In each of these events, communication is the key to ensuring successful navigation through events with significant operational impact. Participants will learn issues to consider for both planned and unplanned disruptions to mission critical operations, and strategies to mitigate their impact.

Various approaches peculiar to each event will be shared. The target audience for this seminar is directors, husbandry and operation managers, supervisors, and animal care staff.

Speakers/Topics:

u

Andrew T Pesek

Welcome and Introductions

Cynthia R Lockworth

Working without Water: How to Successfully Manage a Water Outage in a Vivarium

Chetna Patel

What Were We Thinking? An EightMonth Cagewash Renovation

Andrew T Pesek

We Did Not Plan for This: A Citywide Water Outage

Stacy A LeBlanc

Don’t Panic: Communication Is the Key to Reducing Anxiety

Old and New Technology in the Mouse House

Leader/Moderator: Judy M Hickman-Davis Facilitator: Toi A Collins

In the era of cell phones that can turn off your home alarm remotely and stream your favorite video at the same time, tracking information with pen and paper feels inefficient. At academic institutions there are obstacles to implementing new technologies. Funding, operating system compatibility and multiple stakeholders provide challenges for employing new systems. Technologies that facilitate communication between managers, caretakers, veterinarians, and scientists promote animal welfare and research. However, many institutions continue to use a paper format for medical records, to rack animal health and to conduct routine husbandry tasks. The ability to notify researchers about animal health issues from the cage side instantly can be accomplished using electronic smart systems. Electronic medical records can increase efficiency while improving animal care. Electronic task management offers the advantage of tracking job completion in real time and allows teams to effectively allocate resources and personnel. However, no single electronic system fits every facility and the need for changes and software updates can be time consuming and expensive. Digital health tools have become a mainstay in many households. The application of Fitbit technology to rodent research can improve monitoring of endpoints and allow for real-time behavior assessments. This noninvasive technology is the next generation of monitoring for lab animals. Large automatic cage washers and robotic systems have improved sanitation efficiency and decreased allergen exposure. The challenges include cost, training, maintenance, and functionality especially when dealing with equipment retrofitted into old spaces adapted for reuse as a vivarium. This session will review technologies developed and/ or in use at different academic institutions. The challenges associated with electronic medical records, development of an operations app to track workflow, development of a “fitbit” for mice as a refinement tool, and difficulties encountered with cage washers and robotics systems will be discussed.

Speakers/Topics: Judy M HickmanDavis

Welcome and Introductions

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

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See us at Booth #1600 kentscientifi c.com/somno | 888.572.8887 | 860.626.1172 qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

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F acility

design , management , and operation

Erin Yu

Electronic Medical Records, Easy or Not So Easy

Joseph D Thulin

Task Management: There’s an App for That

Ravi J Tolwani

The Rodent Fitbit

Carrie L Freed

Sanitation with Cage Washers and Robots, the Lessons Learned

u The TNPRC Regional Biosafety Laboratory: A Vital National Resource Speaker: Edwin S Kreitlein Moderator: Luis M Zorrila Facilitator: TBN

In February, the Tulane National Primate Research Center (TNPRC) became one of the first research facilities in the nation to receive authorization from the CDC to obtain specimens of SARS-CoV-2, the virus known as COVID-19. With more than 142,600 confirmed cases in 135 countries with at least 5,400 deaths by the mid-March 2019, this highly infectious virus has rapidly become a threat to health across the globe. All the work associated with the virus conducted at the TNPRC will be performed in the Regional Biosafety Laboratory (RBL). As the largest facility of its kind in the nation, this highly sophisticated, NHP, biomedical research facility was designed to the requirements of ABSL-3 design guidelines established by the NIH and the CDC. The facility has played an important role in the discovery of vaccines developed to support the health programs of the country, including research on the coronavirus. This presentation will focus on key components of the RBL, and how they work together to assist staff to complete the important tasks they necessarily need to execute. Completing these tasks are done so under extremely strenuous working conditions. Among the items that will be discussed are an aerobiology suite, telemetry monitoring, surgical functions, pathology, cage processing and sterilization, tissue digestion, lab functions, and sustainability/energy saving applications. Planning concepts, images, and details will be shared with attendees by the lead lab planner who was involved in the realization of this extremely important national resource from the generation of the grant that funded the facility to its inception. Participants will learn about the nuances of designing a facility of this complex nature, and in so doing obtain a understanding and appreciation for their role in the research and discovery of solutions that prevent the costly impact that emerging infectious diseases have on humanity.

When the Odds Are not in Your Favor: Lessons Learned from Small-scale Disasters with Large-scale Implications u

Leaders: Lesley A Colby, Lois A Zitzow Moderator: Lesley A Colby Facilitator: Emily S Reimold

Disasters happen. Yet when most lab animal professionals plan for disasters, they envision large-scale natural disasters such as hurricanes, wide-spread flooding, and earthquakes. The likelihood that these disasters will occur is partially determined by geography and, thankfully, happen infrequently. In contrast, small-scale disasters can and do occur at multiple institutions each year. Like large-scale events, these local incidents can endanger human health and animal welfare and can significantly disrupt animal research and facility operations. Managing these disruptions can be especially challenging when staff are expected to relocate animals and maintain normal operations in unaffected areas. In addition, without a clearly recognizable large-scale event, institutions and local authorities may struggle in determining their respective roles and responsibilities while managing isaster response. Participants will learn important concepts and strategies regarding disaster planning and management as speakers describe recent disasters affecting their animal facilities. The seminar emphasizes what personnel can do now to best prepare for and then manage small-scale disasters. The target audience includes animal husbandry, management, and veterinary care personnel, as well as biosafety professionals.

Speakers/Topics: Lesley A Colby

Welcome and Introduction

Lesley A Colby

Too Hot to Handle: Breach of Radioactive Source Materials during Irradiator Decommissioning

Richard B Huneke

It All Started with a Spark: How an Animal Facility Storeroom Fire Disrupted Research for Six Months

John N Norton

When It All Goes Boom: A Case Study of the Unexpected Disaster

Lois A Zitzow

(Almost) at the Epicenter: the SARS-Cov-2 Outbreak in Singapore

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

O vercoming C hallenges An International Perspective on Managing the COVID-19 Pandemic in Laboratory Animal Care Programs

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Pandemics are large-scale outbreaks of infectious diseases that can increase morbidity and mortality in humans and result in significant economic, social, and political disruptions. Evidence suggests that the incidence of pandemics will increase over the next century because of increased global travel, urbanization, industrialization, and greater exploitation of natural environments. Since 2003, the world has faced epidemics of SARS-CoV, MERS-CoV, H5N1, and H7N9, as well as a pandemic of H1N1. The recent COVID-19 pandemic has challenged many organizations throughout the world and highlighted significant vulnerabilities and lack of preparedness at the federal, state, and local levels. Universities are particularly susceptible to the effects of an infectious disease outbreak due to the nature of their operations that rely on a large diverse work force, often including students, to manage their academic and research endeavors. Business continuity of animal care and use programs during an outbreak becomes a significant challenge and requires close coordination between numerous actors, including institutional officials, IACUC members, researchers, animal care program leaders, and an entire supply chain to ensure the health and wellbeing of research animals and preserve animal based research programs. We have gathered international leaders in the laboratory animal care communities from Asia, Europe, and the United States to reflect on the management of the COVID-19 crisis and determine plans that will support animal care programs in dealing with future outbreaks. The target audience is individuals at all organizational levels, including directors, managers, and supervisors.

Challenges Credentialed Veterinary Technicians Face in Laboratory Animal Science

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Leader/Moderator: Ann L Murray Facilitator: Amanda A Bedwell Panelists: Victoria R Elam, Jodi M Ternes, Beth Ann Skiles, Julie A Avalos

Credentialed veterinary technicians (CVT, LVT, RVT) make up a significant cross-section of laboratory animal science professionals. As graduates of an AVMA-accredited program that have successfully passed the Veterinary Technician National Exam, these veterinary technicians have a diverse and unique skill set that set them apart from others in the field. As animal husbandry staff, health technicians, surgery technicians, IACUC coordinators, facility and/or lab managers, credentialed veterinary technicians face day-to-day challenges in terms of education, experience, job satisfaction, recognition, physical and emotional toll, and career advancement. Our panel of dedicated professional credentialed veterinary technicians will review the challenges in regulatory record keeping, share tips on transitioning career paths in research, and provide perspective on compassion fatigue. Participants will learn they are not alone trying to overcome hurdles but to use these as opportunities for growth, as well as to provide proven advice to help navigate their current career path.

This Panel Discussion is sponsored in part by Academy of Laboratory Animal Veterinary Technicians and Nurses (ALAVTN) and Society of Laboratory Animal Veterinary Technicians (SLAVT).

Creative and Economical Solutions for an Occupational Health Program

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Speaker: Lisa M Kelly Moderator: Natasha Melfi Facilitator: TBN

The provision of preventative occupational health services is an expectation for institutions conducting animal-based research. The Guide for the Care and Use of Laboratory Animals, indicates that occupational health and safety “is an essential part of the overall Program of animal care and use.” Animal care staff are our most

Finding Homes for Our Animal Heroes: Overcoming Challenges in Research Animal Adoption

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Leader/Moderator: Lauren Brierly Facilitator: TBN Panelists: Abigail Wolf Greenstein, Patti Strand, Jessica M Izzi, Meghan A Connolly

Adoption of research animals is becoming an ethical and, in some states, a legal standard. However, there are many challenges and considerations associated with establishing, expanding, and maintaining a successful animal adoption program.

Panelists from academia, government, and industry will discuss the specific challenges they have faced in research animal adoption and how their organizations have confronted those challenges. Topics include reducing security risk and appropriate advertising, managing staff time and other resources, creating a culture that supports adoption, choosing animals and suitable adopters, costs and considerations when preparing an animal for adoption, and how to talk about your adoption program with the public. Following short presentations, panelists will answer questions from the audience. The target audience is anyone interested in learning more about establishing, expanding, and maintaining a research animal adoption program. m

Keeping Your Culture of Care Current

Speaker: Joy Redmond Moderator: Ghislaine Poirier Facilitator: Michael A Koch

The target audience is anyone interested in further defining and developing their culture of care. This presentation is intended to take you on a journey from measuring your current culture, to developing a vision for the future, to implementation of that vision, and then onward to encouraging future growth of the culture.

We will approach the process like creating a perpetual road map. For your journey you will need to determine: 1. Where are you now? 2. Where do you want to go?

3. How do you want to get there? 4. How do you keep the journey ongoing? This presentation will provide some ideas on how to address the process. It includes topics such as creating an inclusive culture of care core team, creating a vision statement, developing an employee survey to provide a baseline of current views, creating new commitments based on survey results, local/global rollout activities, and ongoing education and plans for continuous reassessment and growth. greatly improve the outcome of the study. Similarly, the selection of an inappropriate or ineffective animal model can be very costly and unrewarding. An ideal animal model must recapitulate the human disease or condition that is being investigated while also having high preclinical translatability to humans. The miniature swine can be an ideal preclinical animal model due to its anatomical, physiological,

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

Leader/Moderator: Mathias Leblanc Facilitator: TBN Panelists: Lois A Zitzow, Lesley A Colby, John N Norton, Alberto Gobbi, Sonja T Chou

valuable resource. It is imperative that employees receive high-quality healthcare services that protect both their physical and mental well-being. However, it is often administratively and financially challenging to institute and maintain such programs. This session will focus on how the University of Georgia (UGA) creatively and economically met these challenges and established a top-notch occupational health and safety program. UGA was able to make use of existing learning management software in unconventional ways. By expanding on the traditional learning paradigm, UGA significantly decreased onboarding days for new employees and expanded services for participants. Additionally, the university established a robust emergency response system that facilitates collaboration between compliance units (e.g. biosafety, chemical safety) and emergency healthcare providers, to encourage a timely prophylactic response for time-sensitive emergencies (e.g. nonhuman primate bite). UGA was also able to partner with other campus stakeholders to develop a respiratory protection program that pooled resources. This session will provide details on how UGA’s occupational health program increased its capacity for services, enhanced the quality of the services provided, and dramatically improved customer satisfaction, all without additional net cost. This session is ideal for anyone interested in how to run a high-quality occupational health program, which prioritizes employee health, on a limited budget.

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AAL AS 71 st N AT I ON AL MEET I N G

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O vercoming C hallenges pathophysiological, and genetic similarities to humans. However, there are many challenges associated with large animal modeling, whether it be a naturally occurring, surgical, chemically induced, or genetically modified produced swine model.

The recognition and use of miniature swine in translational research continues to grow. Translational research where miniature swine are having an impact include cardiovascular and medical device intervention, behavior and cognitive function, dermal research, gene-edited models of human diseases, and neurology. We expect a broad audience will benefit from these presentations, including laboratory animal veterinarians, attending veterinarians, veterinary and animal technicians, facility managers, graduate students, and scientists interested in miniature swine as a model of translational research.

similarities to humans. However, there are many challenges associated with large animal modeling, whether it be a naturally occurring, surgical, chemically induced, or genetically modified produced swine model. The recognition and use of miniature swine in translational research continues to grow. Translational research where miniature swine are having an impact include cardiovascular and medical device intervention, behavior and cognitive function, dermal research, gene-edited models of human diseases, and neurology. We expect a broad audience will benefit from these presentations, including laboratory animal veterinarians, attending veterinarians, veterinary and animal technicians, facility managers, graduate students, and scientists interested in miniature swine as a model of translational research.

This Seminar is sponsored in part by Sinclair Bio Resources, LLC.

Overcoming Challenges in Miniature Swine Translational Research

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Leader: Michael Swindle Moderator: Derek Brocksmith Facilitator: Vikki Wehmeier

Speakers/Topics:

The positive outcome of translational research depends on the study conduct and the selection of the best animal model. Large animals, particularly the miniature swine, represent several unique challenges to the investigator. The proper handling, husbandry, care, and behavior enhancement will greatly improve the outcome of the study. Similarly, the selection of an inappropriate or ineffective animal model can be very costly and unrewarding. An ideal animal model must recapitulate the human disease or condition that is being investigated while also having high preclinical translatability to humans. The miniature swine can be an ideal preclinical animal model due to its anatomical, physiological, pathophysiological, and genetic

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Michael Swindle

Welcome and Introductions

Michael Swindle

Better Modeling of Cardiovascular Disease and Medical Device Intervention Swine

Derek Brocksmith

Improving the Human-Animal Bond and Study Outcome through Behavior Enhancement

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qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

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O vercoming C hallenges Guy Bouchard

Improvements of Using Miniature Swine as a Research

Douglas R Kern

Advancement in Swine Gene Editing and Modeling of Human Disease

Overcoming Challenges of Pain Management in Rodents

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Leader/Moderator: Mark A Suckow Facilitator: TBN

ponotic concerns of the virus to research animals are being considered. This panel discussion will provide information and facilitate discussion on animal program preparations in the face of SARS CoV-2 outbreak. Basic information about the virus will be provided followed by national outbreak updates and contingency plans of representative programs in China, Singapore, Germany, and the United States. We will encourage attendees to share their facility preparations, experiences, and thoughts regarding COVID-19. Target audience include those in animal care and use program management, veterinarians, technicians, and IACUC and environmental health and safety personnel.

Promoting Animal Welfare Through Improvements in Nonhuman Primate Caging Designs

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The reduction of pain and distress experienced by rodents derives from an ethical imperative to ensure humane care. In this regard, an understanding of pain is critical, including recognition and approaches to pain control, as well as the implications of both with respect to research outcomes. The importance of this ethical imperative is further emphasized by 2020 being designated by the International Association for the Study of Pain as the Global Year for the Prevention of Pain.

An important overarching challenge is the application of strategies that reflect agreement between veterinary and animal care professionals and scientists which synergize sound animal care with scientific requirements. Participants will gain basic understanding of how pain develops and becomes aversive to animals; how to recognize pain and strategies for pain reduction; and the impact of pain and pain reduction on research outcomes. This seminar is intended for veterinarians, animal care technicians, IACUC members, and scientists.

Speakers/Topics: Mark A Suckow

Welcome and Introductions

Mark A Suckow

Regulatory and Ethical Underpinnings of Pain Management

Christina M Larson

Causes and Mechanisms of Pain: A Brief Overview

Patricia V Turner

Assessment of Pain and Distress

Jennifer L Lofgren

Management of Pain in Rodents

Patricia L Foley

Achieving Consensus: Working with the Research Team to Build Synergistic Strategies

Pandemic 101: Animal Care and Use Programs Face COVID-19

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Leader/Moderator: Jason S Villano Facilitator: Rachel E Cooper Panelists: Susan R Compton, Scout T Chou, Bryan E Ogden, Gabi Itter, Eric K

Hutchinson COVID-19, the illness caused by SARS CoV-2, caught the world by surprise. Its human health, societal, and economic impact continue to climb to staggering heights, especially as countries continue to grapple with the difficulty of outbreak management of a novel virus. This impact has certainly reverberated to the animal research community and posed unique challenges to disaster plans of animal care and use programs. Continuity of veterinary and husbandry services and research studies could be in peril. Availability of supplies like personal protective equipment, animal feed, drugs, and experimental compounds is being questioned. Anthro-

Leader: John J Hasenau, Monika A Burns, Lawrence Williams, LaVonne D Meunier,

Kristine Coleman, Dawn M Abney Moderator: John J Hasenau Facilitator: TBN Panelists: John J Hasenau, Monika A Burns, Lawrence Williams, LaVonne D Meunier, Kristine Coleman, Dawn M Abney The panel will discuss nonhuman primate (NHP) caging, focusing on historical, current, and future indoor caging designs that promote animal welfare and facilitate study procedures. Cage space allotments and differences between North American and European standards have indicated the need for well-designed, performance-based studies to assess space utilization and welfare. Discussions will concentrate on key design considerations for existing and new mobile and fixed caging for New World and macaque species. Behaviorists with NHP expertise will discuss how animal environments and cage design impact species typical behaviors and improve welfare. Results from an Association of Primate Veterinarians/ American Society of Primatologists survey on caging standards will be included.

Current caging enhancements for specific study-related aspects (aging studies, neurological studies, and toxicology studies) will be discussed. Audience questions and discussion will be encouraged throughout the session. Panel participants have many years of experience with different caging and are knowledgeable about NHP behavior performance standards. The target audience includes all participants of the NHP user community (technicians, scientists, veterinarians, behaviorists, administrators) to enhance their knowledge of NHP caging design and promote the welfare of NHPs with design features.

This Panel Discussion is sponsored in part by Association of Primate Veterinarians (APV).

Raising the Next Generation of Lab Animal Professionals

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Leader/Moderator: Judy M Hickman-Davis Facilitator: Toi A Collins

Employment of veterinarians, veterinary assistants, and animal caretakers in the laboratory setting is predicted to grow 18-19%, resulting in good job opportunities.

The ability to recruit and retain lab animal care providers is essential for providing quality animal care. The opportunity to introduce lab animal care as a profession early in the education process can open the door for identifying quality individuals. Involvement in community programs for high school students provides an opportunity for discussion about careers supporting animal welfare and science.

Veterinary technical schools include lab animal medicine as part of their training and partnering with these colleges to host externships can serve as a source for trained veterinary technicians. The availability of undergraduate students for fullor part-time animal care support can serve a dual purpose of filling a workforce need while introducing rewarding career options. The American Society for Lab Animal Practitioners (ASLAP) provides resources and programs for veterinary students and veterinarians in the form of scholarships, student chapter support, lectures, summer fellowships, and contacts for training programs. The opportunity

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

O vercoming C hallenges

Speakers/Topics: Judy M HickmanDavis

Welcome and Introductions

Valerie K Bergdall

The Relationship between Community Outreach and Technical Support

Christopher S King

Student Workers, Higher Education, and Good Animal Care

Carrie L Freed

Supporting Laboratory Animal Medicine for the Veterinarian

David Lee-Paritz

Policy and the Human-Animal Bond

Six Degrees of Separation: How to Expand Your Network in a Meaningful Way

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Leader: Tasha M Thomas Moderator:Â Ann L Murray Facilitator:Â TBN

There is a concept called six degrees of separation, which concludes that all people are only six or less social connections away from each other. This is also known as the 6 handshakes rule. Imagine anything you wanted to pursue or accomplish in life, and that the resources, or specifically the people with the resources, were only 6 handshakes away. More importantly, imagine having the skillset or knowledge that could support someone else? Essentially, someone is looking for someone just like you and there is someone out there right now with a solution for you. As a result, any two people in a maximum of six steps can be connected. Even with social distancing in place, a powerful and diverse network can be maintained through several social media platforms when traditional methods are not practical. Consider your employment options against your current network. Some experts say that 70 percent of people ended up in their current position thanks to networking.

Others say it is even higher. But networking is more than just looking for a job. The panelists will share what is networking, why it is important, and when is the best time to network. Panelists will dive into the concept of six degrees of separation, the power of networking, building relationships, and share tips on how to get out of your comfort zone and stay connected. The target audience is industry professionals at all levels who can benefit from a diverse and powerful network.

The presentation will appeal to both junior and senior staff with a desire to grow, collaborate, and exchange knowledge and resources to improve their personal and professional lives.

qAnimal Welfare, Regulatory Compliance, and Public Education vBiomedical Research, Medicine, and Methodology uFacility Design, Management, and Operation m Overcoming Challenges

AAL AS 71 st N AT I ON AL MEET I N G

for graduate student training in animal welfare and public policy promotes a better understanding of the human-animal interaction and fills the gap for necessary regulatory support. This session will review practices at academic institutions for introducing lab animal medicine to high school students, veterinary technicians, undergraduates, veterinary students, and graduate students. Opportunities to engage area high school students and veterinary technical colleges, utilization of undergraduate students as part of the essential workforce, the role of ASLAP in veterinary student and veterinarian training, and graduate training opportunity will be discussed. The target audience for this seminar is animal program directors, veterinarians, and facility managers/supervisors. Anyone who might be involved in public outreach, employee recruitment, veterinary student or technician education can benefit from this seminar.

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WARNING: ABUSE POTENTIAL, LIFE-THREATENING RESPIRATORY DEPRESSION, and ACCIDENTAL EXPOSURE Abuse Potential This formulation contains buprenorphine, a high-concentration (1.3 mg/mL) opioid agonist and Schedule III controlled substance with an abuse potential similar to other Schedule III opioids. The high concentration may be a particular target for human abuse.

Buprenorphine has opioid properties that in humans may lead to dependence of the morphine type. Abuse of buprenorphine may lead to low or moderate physical dependence or high psychological dependence. The risk of abuse by humans should be considered when storing, administering, and disposing of Ethiqa XR. Persons at increased risk for opioid abuse include those with a personal or family history of substance abuse (including drug or alcohol abuse or addiction) or mental illness (suicidal depression). Because of human safety risks, this drug should be used only with veterinary supervision.

Do not dispense Ethiqa XR™.

Life-Threatening Respiratory Depression The concentration of buprenorphine in Ethiqa XR is 1.3 mg/mL. Respiratory depression, including fatal cases, may occur with abuse of Ethiqa XR. Ethiqa XR has additive CNS depressant effects when used with alcohol, other opioids, or illicit drugs that cause central nervous system depression. Because of the potential for adverse reactions associated with accidental injection, Ethiqa XR should only be administered by a veterinarian or laboratory staff trained in the handling of potent opioids.

Important Safety Information for Rats and Mice For Rats and Mice: Only administer Ethiqa XR by subcutaneous injection. Ethiqa XR is not intended for intravenous, intra-arterial, intrathecal, intramuscular, or intra-peritoneal injection. Do not use on mice or rats with pre-existing respiratory deficiencies.

Do not keep rats on wood chip-type bedding after administration of Ethiqa XR. Use caution with concomitant administration of Ethiqa XR with drugs that cause respiratory depression.

For Humans: Ethiqa XR should only be administered by a veterinarian or laboratory staff trained in the handling of potent opioids. Protective clothing is recommended to avoid direct contact with human skin or mucus membranes which could result in aborption of buprenorphine and adverse reactions.

Not for use in humans.

For more information, consult the Prescribing Information including the boxed warning located on the next page. ethiqaxr.com 833-EthiqaXR (833-384-4729) © 2020 Fidelis Pharmaceuticals, LLC September 2020 FID-ETH-024

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Opioid Analgesic For subcutaneous use in mice and rats only.

CAUTION: Federal law restricts this drug to use by or on the order of a licensed veterinarian.

LEGAL STATUS--In order to be legally marketed, a new animal drug intended for a minor species must be Approved, Conditionally Approved, or Indexed by the Food and Drug Administration. THIS PRODUCT IS INDEXED--MIF # 900-014.

Extra-label use is prohibited.

This product is not to be used in animals intended for use as food for humans or food-producing animals.

WARNING: ABUSE POTENTIAL, LIFE-THREATENING RESPIRATORY DEPRESSION, and ACCIDENTAL EXPOSURE Abuse Potential Ethiqa XR contains buprenorphine, a high concentration (1.3 mg/mL) opioid agonist and Schedule III controlled substance with an abuse potential similar to other Schedule III opioids. The high concentration of Ethiqa XR may be a particular target for human abuse. Buprenorphine has opioid properties that in humans may lead to dependence of the morphine type. Abuse of buprenorphine may lead to low or moderate physical dependence or high psychological dependence. The risk of abuse by humans should be considered when storing, administering, and disposing of Ethiqa XR. Persons at increased risk for opioid abuse include those with a personal or family history of substance abuse (including drug or alcohol abuse or addiction) or mental illness (suicidal depression).

Because of human safety risks, this drug should be used only with veterinary supervision. Do not dispense Ethiqa XR. Life-Threatening Respiratory Depression The concentration of buprenorphine in Ethiqa XR is 1.3 mg/mL. Respiratory depression, including fatal cases, may occur with abuse of Ethiqa XR. Ethiqa XR has additive CNS depressant effects when used with alcohol, other opioids, or illicit drugs that cause central nervous system depression.

Because of the potential for adverse reactions associated with accidental injection, Ethiqa XR should only be administered by a veterinarian or laboratory staff trained in the handling of potent opioids.

DESCRIPTION Ethiqa XR is an injectable suspension of extended-release buprenorphine. Buprenorphine hydrochloride, an opioid analgesic, is the active ingredient in Ethiqa XR. Lipid-bound buprenorphine hydrochloride is suspended in medium chain fatty acid triglyceride (MCT) oil. Lipids encapsulate the buprenorphine limiting diffusion which provides for larger doses and prolonged action.1,2 Ethiqa XR has a slightly yellow to white opaque appearance. Each mL contains approximately 1.3 mg buprenorphine hydrochloride. The sterile product contains cholesterol, glyceryl tristearate, and buprenorphine hydrochloride suspended in MCT oil.

Buprenorphine Formula C29H41NO4

INDICATIONS Ethiqa XR is indicated for the control of post-procedural pain in mice and rats.

MOUSE DOSAGE AND ADMINISTRATION Wear protective clothing when administering Ethiqa XR (see Human Safety Warnings).

Shake the vial briefly before each use to ensure uniform suspension. If stored refrigerated, bring to room temperature before use.

Use aseptic techniques to withdraw the dose into a disposable 0.5 or 1 mL syringe. A 20 to 23 gauge needle should be used for injections due to the viscosity of the drug suspension.

The dosage of Ethiqa XR is a single subcutaneous injection of 0.05 mL per 20 gram mouse (3.25 mg/kg body weight).

Therapeutic drug concentrations are maintained for 72 hours after the initial dose. If needed, a single repeat dose may be administered 72 hours after the initial dose.

Secure the mouse in a scruff-of-the-neck hold. Insert the needle into the dorsal subcutaneous space created by the scruff hold.

Inject the entire dose into the dorsal subcutaneous space. An oily sheen may be observed in the dorsal fur of the mouse after injection due to leakage of the oil-based drug suspension from the injection site. The oily sheen may last for 4 to 5 days postinjection. Leakage from the injection site can be minimized by slowly injecting Ethiqa XR into the subcutaneous space. The mouse can be returned to its cage immediately after receiving Ethiqa XR. Do not return any unused drug suspension from the syringe back into the vial.

Once the vial is broached, Ethiqa XR can be stored at 15° to 25°C (59° – 77°F) or refrigerated for 28 days. DO NOT FREEZE. RAT DOSAGE AND ADMINISTRATION Wear protective clothing when administering Ethiqa XR (see Human Safety Warnings).

Shake the vial briefly before each use to ensure uniform suspension. If stored refrigerated, bring to room temperature before use.

Use aseptic techniques to withdraw the dose into a disposable 0.5 or 1 mL syringe. A 20 to 23 gauge needle should be used for injections due to the viscosity of the drug suspension.

The dosage of Ethiqa XR is a single subcutaneous injection of 0.1 mL per 200 gram rat (0.65 mg/kg body weight). Therapeutic drug concentrations are maintained for 72 hours after the initial dose. If needed, a single repeat dose may be administered 72 hours after the initial dose.

Secure the rat in a passive restraint tube or by holding with a heavy glove with one person to secure the rat and a second person to administer the drug. Insert the needle in the dorsal subcutaneous space. Inject the entire dose into the dorsal subcutaneous space. An oily sheen may be observed in the dorsal fur after injection due to leakage of the oil-based drug suspension from the injection site. The oily sheen may last for 4 to 5 days post-injection. Leakage from the injection site can be minimized by slowly injecting Ethiqa XR into the subcutaneous space. The rat can be returned to its cage immediately after receiving Ethiqa XR. See CONTRAINDICATIONS and Rat PRECAUTIONS for additional information on bedding.

Do not return any unused drug suspension from the syringe back into the vial.

Once the vial is broached, Ethiqa XR can be stored at 15° to 25°C (59° – 77°F) or refrigerated for 28 days. DO NOT FREEZE. CONTRAINDICATIONS Only administer Ethiqa XR by subcutaneous injection. Ethiqa XR is not intended for intravenous, intra-arterial, intrathecal, intramuscular, or intra-peritoneal injection.

Do not use on mice or rats with pre-existing respiratory deficiencies.

Do not keep rats on wood chip-type bedding after administration of Ethiqa XR. HUMAN SAFETY WARNINGS Not for use in humans. Keep out of the reach of children.

Human User Safety while handling Ethiqa XR: Two trained staff for administration: Ethiqa XR should only be handled and administered by a veterinarian, veterinary technician, or laboratory staff trained in the handling of potent opioids. To prevent human adverse reactions or abuse, at least 2 trained administrators should be present during injection of Ethiqa XR. Protective covering: To prevent direct contact of Ethiqa XR with human skin or mucous membranes when handling the suspension, protective clothing is recommended.

Mucous membrane or eye contact during administration: Direct contact of Ethiqa XR with the eyes, oral or other mucous membranes of humans could result in absorption of buprenorphine and the potential for adverse reactions. If accidental eye, oral or other mucous membrane contact is made during administration, flush the area with water and contact a physician.

Skin contact during administration: If human skin is accidentally exposed to Ethiqa XR, wash the exposed area with soap and water and contact a physician. Accidental exposure could result in absorption of buprenorphine and the potential for adverse reactions.

Drug Abuse, Addiction, and Diversion of Opioids: Controlled Substance: Ethiqa XR contains buprenorphine, a mu opioid partial agonist and Schedule III controlled substance with an abuse potential similar to other Schedule III opioids. Ethiqa XR can be abused and is subject to misuse, abuse, addiction,

and criminal diversion. Ethiqa XR should be handled appropriately to minimize the risk of diversion, including restriction of access, the use of accounting procedures, and proper disposal methods, as appropriate to the laboratory setting and as required by law.

Abuse: Abuse of Ethiqa XR poses a hazard of overdose and death. This risk is increased with concurrent abuse of alcohol and other substances including other opioids and benzodiazepines. Buprenorphine has been diverted for non-medical use into illicit channels of distribution. All people handling opioids require careful monitoring for signs of abuse. Drug abuse is the intentional non-therapeutic use of a prescription drug for its rewarding psychological or physiological effects. Abuse of opioids can occur in the absence of true addiction.

Storage and Discard: Ethiqa XR is a Class III opioid. Store in a locked, substantially constructed cabinet according to DEA and local controlled substance guidelines. Discard broached vials after 28 days. Any unused or expired vials must be destroyed by a DEA registered reverse distributor; for further information, call 1-833-384-4729.

Physician information: Ethiqa XR injectable suspension is a mu-opioid partial agonist (1.3 mg buprenorphine/mL). In the case of an emergency, provide the physician with the package insert. Naloxone may not be effective in reversing respiratory depression produced by buprenorphine. The onset of naloxone effect may be delayed by 30 minutes or more. Doxapram hydrochloride has also been used as a respiratory stimulant.

PRECAUTIONS Mice The safety of Ethiqa XR has not been evaluated in pregnant, lactating, neonatal, or immune-compromised mice. As with other opioids, buprenorphine may cause sedation, decreased blood pressure, decreased heart rate, decreased gastrointestinal mobility, and respiratory depression. Use caution with concomitant administration of Ethiqa XR with drugs that cause respiratory depression. The use of paper or soft bedding for up to 3 days following administration of Ethiqa XR should be considered.

Normal mice may exhibit an obtunded response to stimuli up to 4 hours after receiving Ethiqa XR. Buprenorphine is excreted in the feces (see Clinical Pharmacology section below). Coprophagy may lead to ingestion of buprenorphine or its metabolites by mice treated with Ethiqa XR and untreated cage mates.

Rats The safety of Ethiqa XR has not been evaluated in pregnant, lactating, neonatal, or immune-compromised rats. As with other opioids, buprenorphine may cause sedation, decreased blood pressure, decreased heart rate, decreased gastrointestinal mobility, and respiratory depression. Use caution with concomitant administration of Ethiqa XR with drugs that cause respiratory depression.

Rats may exhibit signs of nausea including pica up to 3 days post-treatment. Rats should be maintained on paper or soft bedding to avoid ingestion of wood chip-type bedding after administration of Ethiqa XR. Pica involving wood chip-type bedding can be lethal in rats.

Buprenorphine is excreted in the feces (see Clinical Pharmacology section below). Coprophagy may lead to ingestion of buprenorphine or its metabolites by rats treated with Ethiqa XR and untreated cage mates.

ADVERSE REACTIONS Mice No adverse reactions were observed in 20 to 25 gram young adult male and female mice after a single subcutaneous injection of Ethiqa XR at a dose 5 times the indicated dose. Laboratory parameters evaluated in the study included hematology and clinical chemistry; histopathology was also performed. In a second study, adult male and female mice received Ethiqa XR subcutaneously at 5 times the indicated dose for three doses at four day intervals. A surgical procedure was performed on the study mice prior to receiving each of the three doses of Ethiqa XR. Mortality was seen in two male mice after the third surgical procedure and dose of Ethiqa XR (total dose of 49 mg buprenorphine/ kg body weight in 8 days).

Weight loss has been observed in mice treated post-procedurally with Ethiqa XR. Rats Adverse reactions were evaluated in 180 to 200 gram young adult male and female rats after a single injection of Ethiqa XR. A surgical procedure was performed on the rats prior to administration of a single dose at the intended dose of 0.65 mg/kg or a single dose of 2, 6 or 10-fold excess dose. Adverse reactions also were evaluated in male and female rats administered 2, 6 and 10 times the intended dose for three doses at four day intervals. A surgical procedure was performed on the rats prior to administration of the first of three doses. Laboratory parameters evaluated in the study included hematology, clinical chemistry, urinalysis, histopathology, and bodyweight.

Signs of nausea were observed at all dose levels within 24 hours of the dose. Signs included self-licking, self-gnawing and efforts to eat wood-chip bedding.

Mortality was seen in 1 of 36 rats exposed to wood chip bedding. Necropsy revealed the stomach and esophagus were compacted with bedding, the bladder was abnormally distended and the urine contained blood.

Mortality was seen in 3 of 222 rats treated with Ethiqa XR due to technical complications with serial bleeding of the jugular vein.

For technical assistance, or to report an adverse drug reaction, please call Fidelis Pharmaceuticals LLC at 1-833-384-4729.

For additional information about adverse drug experience reporting for animal drugs, contact FDA at 1-888-FDA-VETS or http:// www.fda.gov/AnimalVeterinary/SafetyHealth.

CLINICAL PHARMACOLOGY3 Buprenorphine can act as an agonist and antagonist at different classes of opioid receptors. Agonism at the mu opioid receptor and, in some cases, antagonism at the kappa or delta opioid receptors are possible underlying mechanisms for the ceiling effect and bell-shaped dose-response curve of buprenorphine. Studies with knockout mice have shown that the antinociceptive effect of buprenorphine, which is mediated primarily by the mu opioid receptor, is attenuated by the ability of the drug to activate the opioid receptor like (ORL-1) receptor. The drug can be described as a ‘full’ and a ‘partial’ agonist at the same receptor depending on the specific assay. There appears to be no ceiling effect for analgesia, but there is a ceiling effect for respiratory depression.

Pharmacokinetic studies with bolus injections of buprenorphine in mice and rats provide similar models. After bolus intravenous administration, plasma levels decline tri-exponentially. The drug is n-deakylated in the liver to norbuprenorphine (NBN), an active metabolite. Studies have shown that glucuronide metabolites of buprenorphine and NBN are also metabolically active, and can approximate or exceed the concentration of the parent drug. Un-metabolized drug excreted in the urine and feces one week after injection was 1.9 and 22.4% of the dose, respectively, and 92% of the dose was accounted for in one week.3 Mice Pharmacokinetic parameters of Ethiqa XR were studied in 6-8 week old male and female Balb/c mice following a single subcutaneous injection of 3.25 mg/kg bodyweight. Clinically significant blood levels were observed up to 72 hours after subcutaneous injection.

Rats Pharmacokinetic parameters of Ethiqa XR were studied in 8 week old male and female Fischer rats following a single subcutaneous injection of 0.65 mg/kg bodyweight. Clinically significant blood levels were observed up to 72 hours after subcutaneous injection.

HOW SUPPLIED Ethiqa XR is supplied in a multi-use glass vial containing 3.0 mL of injectable drug suspension.

Ethiqa XR

3 mL vial

NDC 86084-100-30

U.S. Patent No. 8,461,173 STORAGE INFORMATION Store between 15° and 25°C (59° – 77°F) or refrigerated. DO NOT FREEZE. If stored refrigerated, bring to room temperature before use. Once broached, the multi-dose vial should be discarded after 28 days.

REFERENCES 1. Mishra et al., Drug Delivery and Transl. Res, 2:238-253; 2012.

2. Bethune et al., The role of drug-lipid interactions on the disposition of liposome-formulated opioid analgesics in vitro and in vivo. Anesth Analg. 93(4):928-33; 2001.

3. Guarnieri et al., Lab Animal, 41(11): 337-343; 2012.

Manufactured for: Fidelis Pharmaceuticals LLC CCIT Incubator 675 US Highway One, Suite B113 North Brunswick, NJ 08902 833-384-4729 www.EthiqaXR.com Fidelis Pharmaceuticals® and EthiqaXR® are registered trademarks of Fidelis Pharmaceuticals LLC, a Delaware Corporation.

February 2020

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LABORATORY INVESTIGATIONS 1 PS2 The Influence of Daytime LED Light Exposure on Fatty Acid and Protein Levels in the Major Metabolic Tissues of Mice RT Dauchy*, LM Dupepe, GL Dobek, AT Pierce, GB Voros, SM Hill, VP Belancio, BB Kiely, EM Dauchy, AA Allen, DE Blask PS3 Exposure to Animal Facility Light at Night Disrupts Circadian Rhythms of Metabolism and Physiology in Nude Mice RT Dauchy*, LM Dupepe, GL Dobek, GB Voros, AT Pierce, BB Kiely, SM Hill, M Anbalagan, DE Blask PS4 Pathogenicity of 2 Corynebacterium bovis Isolates in NSG Mice K Szilagyi*, J Liu, AK Bloom, M Granitar, GE Sandusky, R Livingston PS5 Evaluation of Ankylosing Enthesopathy in C57BL/6J Male Mice BL Kick*, L Anderson, Z Bichler, R Doty, J Wotton PS6 Development and Characterization of the Ultra-immunodeficient NOD.CB17-Prkdcscid IL2rgtm1/ Bcgen (B-NDG) Mouse Model J Naden*, J Xiang

HUSBANDRY/MANAGEMENT PS8 From Vivarium to Hospital: Safeguarding Animals and People in the Face of COVID-19 LE Riddle*, KD Bean, CV Rose, CM Bell PS9 A Collaborative Solution for Animal Care Technician Recruitment S Ogeka*, L Muca, M Morrison, K Krueger PS10 Rapid Buildup of Intracage Ammonia (NH3) in Small Volume Mouse IVC Cages: A Husbandry Challenge MA Eskandarani, O Kalliokoski, J Hau* PS11 Space Is at a Premium: A Real-time Graphical Tool for Cage Space Allocation Planning D Hanson*, H Manning, M Terrasi PS12 Forays into Farrowing Sow: Extra-large Swine in a Modest Facility A Hicks-Nelson*, S Baker

PS13 Two Case Management Lessons Learned from Gas-Bubble Disease in Xenopus laevis: Practical Problem-Solving in an Aquatic Facility NJ Fabian*, S Muthupalani, NY Chan, O Paugois, C Bresilla, CS McCoy, OL Franco Mahecha, JG Fox, D Annamalai PS14 Enrichment Innovation: Human Team Building Activity Benefits Nonhuman Primates B Culp* PS15 Challenges of Capturing, Transporting, Housing, and Safely Handling Wild Caught Juvenile Alligators: Husbandry Considerations and Adaptations For Successful Research RK Banks* PS16 Reusable Surgical Masks in the Face of Supply Shortages K Brannick*, MA Robinson, C Janssen, T Gomez PS17 Chapparvovirus/Mouse Kidney Parvovirus Eradication by Cross-foster Rederivation P Sharp*, I Faseeh, K Greca, A Frost, G Manship

ANIMAL WELFARE, TRAINING, AND THE 3RS PS18 Public Outreach at Work: Kids Allowed!

AT Pierce* PS19 Modern Teaching Methods Increase Employee Success KL Brackett*, A Schile PS20 Reimagining Training and 3Rs through Extended Reality E Crooker*, S Baran PS21 Evaluation of Various Handling Methods and Frequencies to Minimize Stress in Rats J Kurpinski*, T Plachta, D Cooper, J Kylie PS24 Treadmill Training of Laboratory Swine J Kim*, R Eder, J Camacho, C Nguyen

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RESIDENT LABORATORY INVESTIGATIONS 1 PS25 Is a Sentinel Program Good for the Detection of Murine Chapparvovirus?

RK Dhawan*, ML Wunderlich, S Jennings, D Nicholson, B Tedesco, S Dayani, KS Henderson PS26 Indicators of Postoperative Pain in Syrian Hamsters (Mesocricetus auratus) AM Edmunson*, FD Duke Boynton, AK Rendahl, A Merley, N Koewler, ML Dunbar, CP Sivula PS27 Anti-G-CSF Antibody Does Not Prevent Bone Marrow B Cell Loss in Murine Norovirus Infected Stat1-/- Mice D Eldridge*, K Mears, C Hsu PS28 Impact of Differing Gut Microbiota Transfer Methods on Model Phenotypes K Gustafson*, A Ericsson, CL Franklin PS29 Evaluation of Goblet Cell-associated Antigen Passages and Tolerogenic Dendritic Cells in the Cystic Fibrosis Mouse Intestine SM Young*, R Woode, NM Walker, L Clarke PS30 Bile Acid Composition Contributes to Metabolic Improvements after Sleeve Gastrectomy in Mice J Tu*, Y Liu, L Ding, E Zhang, Z Fang, L Jin, W Huang PS31 Evaluation of Various Individually Ventilated Cage Systems Based on Mouse Reproductive Performance and Husbandry and Environmental Parameters MG Stover*, JS Villano PS32 The Influence of Daytime LED Light Exposure on Sprague Dawley Rats AA Allen*, AT Pierce, RT Dauchy, GB Voros, GL Dobek PS33 PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance W Hanson*, K Taylor, DK Taylor PS34 Differences in Strain Susceptibility and Shedding of Murine Chapparvovirus in CD-1, C57BL/6, and NSG Mice ML Kain*, S Monette, RJ Ricart Arbona, KS Henderson, RK Dhawan, M Wunderlich, NS Lipman

LABORATORY INVESTIGATIONS 2 PS35 Pulmonary Delivery of Test Articles by Intratracheal Nebulization or Liquid Instillation in Small Animal Models: implications for Covid19 Therapeutic Testing A Werts*, T Yellowhair, P Kuehl PS39 Development of a COVID-19 Multiplex for Serological Screening of Nonhuman Primate Colonies RK Dhawan*, M Wunderlich, L Campbell PS40 Comparative Genomic Analysis of Novel Helicobacter pylori Strains Isolated from Domestic Cats with Gastritis Reveals a Unique Genetic Profile that May Contribute to Persistent Colonization and Pathogenicity in Nonhuman Hosts A Mannion*, Z Shen, JG Fox PS41 Comparison of the Fecal Bacterial Microbiota of Rhesus Macaques (Macaca Mulatta) by Housing Type and Health Status NR Compo*, L Mieles Rodriguez, D Gomez PS42 The Use of Gnotobiotic Piglets as a Model for Human Norovirus (HuNoV) Infection and Recombinant PIV5 Vector Vaccine Efficacy Z Chen, Y Ma, J Risalvato*, Z Li, H Wei, J Zengel, M Lu, A Li, X Liang, E DiCaprio, R Jennings, A Niehaus, J Li, B He

RESIDENT LABORATORY INVESTIGATIONS 2 PS43 Comparison of Laboratory Disinfectants in their Effect on the Mouse Gut Microbiota JD Sciurba*, GE Chlipala, SJ Green, MA Delaney, JD Fortman, JE Purcell PS44 Effects of Cisapride, Buprenorphine, and Their Combination on Gastrointestinal Transit in New Zealand White Rabbits ER Feldman*, B Singh, N Mishkin, E Lachenauer, M Martin-Flores, EK Daugherity PS45 The Use of Midazolam as an Appetite Stimulant and Anxiolytic in the Common Marmoset (Callithrix jacchus) JA Herrod*, J Avelino, K Schonvisky, J Lynch, EK Hutchinson, JM Izzi PS46 Pharmacokinetics of Single-Dose Intramuscular Buprenorphine in Common Marmosets (Callithrix jacchus) NJ Fabian*, DE Moody, WB Fang, JG Fox, MA Burns

P latform S essions PS48 Effects of Pair Housing on Behavior, Cortisol, and Clinical Outcomes after Intrafacility Transfer and Acclimation in Rhesus Macaques (Macaca mulatta) MN Jackson*, M Truelove, K Williams, J Chen, RH Moore, JS Wood, JK Cohen, M Bloomsmith PS49 Comparisons of Intervertebral Disc Metabolic Responses between Chondrodystrophic and Non-chondrodystrophic Dogs NN Lee*, JS Kramer, AM Stoker, JL Cook PS52 Optimizing Food Accessibility During Zebrafish Rearing Improves Growth, Survival, and Breeding Performance TA Collins*, S Cabrera, E Teets, JL Shaffer, BW Blaser

WHAT’S YOUR DIAGNOSIS? PS53 Sudden Clinical Decline in an Experimentally Naïve Yorkshire Pig AL Carlson*, S Kitz, C Cheleuitte, A Michel PS54 Fluffy, White Growth on a Captive Freshwater Cichlid M Reichert*, N Koewler PS55 Neurologic Signs in a Zebra Finch (Taeniopygia guttata) RJ Floyd*, S Monette, LL Diaz PS56 Bump on the Rump in a Bunny A Skorupski*, IL Bergin, PA Lester PS57 Ulcerative Glossitis and Mortality in Neonatal Muntjac Deer (Muntiacus reevesi) E Houston*, J Helbling, M Burton, E McNulty, JD Ayers, LV Kendall, C Mathiason PS58 Hematochezia in a Rhesus Macaque (Macaca mulatta) L Birdwell*, R Stammen, S Kirejczyk, J Jenkins PS59 Subcutaneous Mass in a Brown-headed Cowbird (Molothrus ater) EM David*, KL Gardiner, AK Brice

PS60 Constipation and Weight Loss in a Rhesus Macaque (Macaca mulatta) E Cho*, PC Smith, S Wilson, JL Asher, K Killoran, CJ Zeiss PS61 Abdominal Pain and Distention in a Ferret SD Alaniz* PS62 Ocular Lesions in a New Zealand White Rabbit LF Mosca*, J Snyder PS63 Hindlimb Lameness in a Pregnant Common Marmoset (Callithrix jacchus) OL Franco Mahecha*, JL Haupt, HR Holcombe, RM Kramer, M Jamiel, JG Fox

CLINICAL PS65 Evaluation of Acute Regurgitation in an Olive Baboon (Papio anubis) S Lane*, D Marchi, W Williams PS66 Planning and Managing Postoperative Care for Spanish Cross Goats Receiving Total Knee Replacement D Marchi*, S Lane, C Rasbach, E Clary, W Williams PS67 Development of a Novel Clinical Scoring System for Corynebacterium bovis Infections for NSG Mice CA Manuel*, EC Pearson, U Pugazhenthi, M Fink, L Habenicht, DL Fong, JK Leszczynski, MJ Schurr PS68 Outbreak of Enteropathogenic E. Coli and Diarrhea in a Colony of New Zealand White Rabbits D Eldridge*, B Iritani PS69 Management of Distal Tibia and Fibula Fracture Using External Coaptation in a Common Marmoset (Callithrix jacchus) AJ McLuckie*, RA Ober, R Donocoff, CL Winnicker PS70 Evaluation of a Continuous Glucose Monitoring System for Use in Diabetic Cynomolgus Macaques (Macaca fascicularis) S Oh*, LC Halliday PS71 Characterization of Gastric Helicobacter spp in a Colony of Research Macaques HR Holcombe*, R Marini, M Patterson, S Muthupalani, Y Feng, AG Swennes, R Ducore, M Whary, Z Shen, JG Fox

AAL AS 71 st N AT I ON AL MEET I N G

PS47 Reference Intervals for Total T4 and Free T4 in Cynomolgus Macaques (Macaca fascicularis) and Rhesus Macaques (Macaca mulatta) JD Sciurba*, JM Hayes, SM Nouraie, HM Wilson, LC Halliday, JD Fortman

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Learn about recent actions taken by the AALAS Board of Trustees and provide feedback to AALAS national leadership by attending your district membership meeting, conducted by your district trustees. They will lead discussion on recent board decisions, issues, policies, and procedures.

Check the list to see which district you’re in and who your trustees are; for example, individuals living in Maryland belong to District 3.

District membership meetings will be held Monday, October 26, from 5:15–6:15 p.m.; room assignments will be listed in the Final Program. International members of AALAS will have a designated meeting room as well and will meet at the same time as the districts.

District 1

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States:

Trustee:

District 2

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States:

Trustee:

District 3

Branches:

States:

Trustee:

Metro New York New England Northern Mountain Southern New England Upstate New York

Delaware Valley New Jersey Three Rivers

Greater Virginia National Capital Area

Connecticut, Maine, Massachusetts, New Hampshire, New York, Rhode Island, Vermont

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Robert Quinn

Pamela Straete

Lisa Secrest

47 District 4

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District 5

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District 6

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District 7

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District 8

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At Large Trustees:

Jason Villano Theresa Woodger Sonja (Scout) Chou James Macy

Central Ohio Indiana Kentucky Michigan Southern Ohio

Central Illinois Chicago Iowa Minnesota Nebraska

Arkansas Kansas City Louisiana Mid-Missouri Mile High Oklahoma Texas

Arizona Hawaii Northern California Northern Rocky Mountain Oregon Sacramento Valley San Diego Southern California Mountain West Washington

Alabama, Florida, Georgia, Mississippi, North Carolina, South Carolina, Tennessee, US Commonwealth of Puerto Rico

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Arkansas, Colorado, Kansas, Louisiana, Missouri, New Mexico, Oklahoma, Texas

Alaska, Arizona, California, Hawaii, Idaho, Montana, Nevada, Oregon, Utah, Washington

Mark Sharpless

Lisa Brossia-Root

Marilyn Watson

Lindsay Holmes

Tom Chatkuptt

AAL AS 71 st N AT I ON AL MEET I N G

Appalachian Caribbean-Puerto Rico Florida Mid-South Research Triangle Southeastern

AAL AS 71 st N AT I ON AL MEET I N G

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POSTERS ANIMAL WELFARE, TRAINING, AND 3RS POSTERS

P12 Development of Standardized Assessment Tools for Evaluation of Animal Care and Use Programs at Contract Research Organizations HN Burr*, A Zuvich, A Lange, W West, NA Bratcher

P1 A Pilot Program Using Positive Reinforcement and Target Training in Ported Yucatan Pigs JL Zorge*, G Arnold, W Gately, S Perrotta

P13 Training and Low Stress Handling Procedures to Reduce Fear during Restraint for Blood Collection in Sprague Dawley Rats C Moody, E Moreau, PV Turner*

P2 Rehoming of Research Animals: Program Implementation, Development, and Benefits A Anguiano-Rosales* P3 Initiative to Promote the 3Rs LM Stanislawczyk*, MC Kundu P4 Improving the Quality of Continuing Reviews: One IACUC’s Journey ML Budda*, AL Burke P5 Lavender Essential Oil Infusion Can Help Reduce Pacing in Rhesus Monkeys (Macaca mulatta) B BoWell* P6 Validation of Mouse Jugular Venipuncture for Serial Blood Collection CR Deugan, NC Masterson* P7 Five-year Outcomes after Implementation of a Critical Incident Reporting System SJ Bischoff*, D Trietschel, A Enkelmann P8 Patency of Jugular and Femoral Vein Catheters Attached to Transcutaneous Buttons in Sprague Dawley Rats with Weekly Maintenance V Karicheti*, L Luciano, SG Camaya, M Koubaitary, MC Pledger, TR Gleason, A Evans, SE Gledhill, PM Sparks P9 Carprofen Delivery via Water-based Gel J Finlay*, L Saganek, J Palmer, TW Adamson, R Ermel P10 Success of a Nonhuman Primate Training Core S Combs*, A Dillard, KR Lambert P11 Harmonizing Recommendation for Enhancing the Care and Wellbeing of Research Pigs across Global Sites E Moreau*, T Bryant, M Wilkinson, PV Turner

P14 Evaluating In-cage Resources for Mice: Nest Materials and a Cardboard Cage Semi-divider for Improving CD-1 and C57BL/6 Mouse Welfare C Moody, E Paterson, PV Turner* P15 The Blues of Teaching Retroorbital Injections: A Refinement to Using Dye as a Validation Technique in Mice JL Ludwig* P16 Technical Refinements in Assisted Reproductive Technologies for the 3Rs P Qi*, OL Franco Mahecha, T Chavarria, E Umana, S Erdman, JG Fox P17 An Innovative Method for Confirming Sexual Maturity in Male Macaques (Macaca fascicularis) C Rosales* P18 A Survey of Laboratory Animal Veterinarians Regarding Mouse Welfare in Biomedical Research J Marx*, K Jacobsen, N Petervary, D Casebolt P19 Identification of Humane Endpoint Markers in Naturally B6 Aged Mice N Ogiso*, K Tomita, K Takano, S Tamura, H Kawasaki, N Morikawa, J Almunia P20 Using Positive Reinforcement to Build Compliance in Sinclair Mini-Pigs JN Perez*, RL Collins, KR Lambert, JW Vaughan P21 Serial Survival Cerebrospinal Fluid Collection and Intrathecal Dose Administration in Intact Rats S Wills, M Krueger*, J Relph, C Gocker, D Lunde, A Larson, V Walsh P22 Species-specific Training Strategies for Vervets C Scott*, KR Lambert, B Frye, D Bissinger, C Copeland, S Wise-Walden, P Valure, H Register, C Shively

POSTERS

P34 Identifying Risk Factors of Self-directed Abnormal Behavior in Rhesus Macaques (Macaca Mulatta) C Widmaier*, K Baker

P24 Characterization of a Novel Stereotypic Behavior in Laboratory Gerbils (Meriones unguiculatus) Housed in Individually Ventilated Caging L Habenicht*, BM Clancy, CA Manuel, DL Fong, A Klug, JK Leszczynski

P35 Applying 3Rs Techniques for Improved Mouse Pseudopregnant Production In a Large Core Facility S Galvez*, R Wang, E Wu, X Rairdan, KD Evans

P25 Successful Repairing of Adult Female Mice with Specialized Feeding Jars V Forck*, K Vandeloecht, M Haney P26 SLAVT/BRAD Partnership: Accelerating Public Awareness and Acceptance of Animal Research AL Murray*, LK France, J Champion, AA Bedwell, PA Clifford P27 Developing a Human-derived iPSC Cerebral Organoid Model for Studies of Pediatric Anesthetic Neurotoxicity C Krall*, M Schaefer, H Hogberg, R Johns P28 When It Comes to Mouse Blood Collection for Inexperienced Users Chin Up, Don’t Get Cheeky DS Tu*, K Nolan, AD Pavan, J Stewart, CL Freed P29 Driving the 3Rs in the Outsourced Space: Innovation and Collaboration MA Burns*, JL Lofgren P30 Refining Transcranial Magnetic Stimulation in Rhesus Macaques M Klores* P31 Refinements to Intermittent Intravenous Infusion Procedures in C57BL/6 Mice to Achieve Study Endpoints and Improve Animal Welfare TR Gleason, A Evans, M Stamen, G Murphy, V Karicheti*

P36 Who’s the Boss? Environmental Influences on Power Distribution in Male Laboratory Mice AJ Barabas*, JR Lucas, BN Gaskill P37 Standardizing Practices between IACUC and IBC to Improve/Maintain Quality Programs V Cuevas* P38 The Jacket with Pulling Power: A Novel Approach to Early Stage Evaluation of Magnetic Nanoparticles AA Ritchie*, PA Clarke, JE Dixon, AM Grabowska P39 A Multimodal Approach to Successful Introduction of Macaca fasicularis Infants to Their Dams Post-cesarean Delivery R Lortz*, D Stockinger, K Mendoza, J Roberts, G Hwa P40 A Guide to Implementing Remote Client Audits: Lessons Learned K Lux* P41 Visual Aide and Demonstration for Estimating Blood Loss in Laboratory Animal Species R Mansour, K Todd, J McInnerney, L Busbin, MS Metzler* P42 A Problem-based Learning Approach to Teaching Animal Welfare Regulations and Oversight TL Whitcomb*, JL Booth, JM Izer, X Peng, C Chang, AN Varley, R Griffin, K Negrini, RP Wilson

P32 Sales, Inventory, and Operations Planning Supports the 3Rs TJ Receveur*, N Bartlett, J Lohmiller

P43 Transcutaneous Buttons Improves Jugular Vein Catheter Bidirectional Patency Duration in LVG Golden Syrian Hamsters V Karicheti, R Velez, A Burgei*, L Bryan

P33 Adapting Positive Reinforcement Training to Novel Laboratory Species A Tresler*, C Rasbach, TL Stevens, W Williams

P44 Veterinary Perspectives on Pain Management for Research Primates E Paterson*, PV Turner

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P23 Use of a Collaborative Housing Tracking System to Increase Rate of First-choice Housing for Large Animal MA Burns*, C Wilbourn, A Lange, S Scott, JL Lofgren

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P45 Hypodermic Needles Used for Multiple Injections in Laboratory Mice T Bennett*, J Rizzo, S Yang, K Field, E Rosfjord

P46 Redesigning a Training Program to Maintain Social Distancing in the Age of COVID-19 M Huss*, E Alamaw, B Franco, C Pacharinsak P47 Strengthening a Hands-on Scientist Training Program in Uncertain Times S Rosati*, B Robinson, L Schmidt, D Getz P48 Why Animal Research: A Teleological Defense from an IACUC Perspective TE Todd* P49 Evaluation of 3 Supervised Machine Learning Models for Recovery Prediction in Ill Rodents F Scorrano* P50 Evaluation of Cost Savings and Adherence to 3Rs with Exhaust Air Duct Sampling for Colony Health Surveillance K McDonald*, E Spofford, R Wilgenkamp, E Bryant-Hall P51 Pharmacokinetics and Assessment of Meloxicam-infused Diet Gel in Comparison with Subcutaneously Injected Meloxicam Using an Incisional Pain Model in Mice R Ober*, Y Berger, GE Geist, CL Winnicker P52 Striving to Strengthen Community Ties and Educate the Public on Animal Research B Fenton*, KA Blanchette, V Cuevas P53 Implementing the 3Rs: A Snapshot of Current Efforts, Barriers, and Needs MR LaFollette*, M Graham, LM Stanislawczyk, JL McMillan

P56 Pools versus Bedding as Enrichment in Grouphoused Macaques: A Splashing Good Time or Confetti for All? M Janavaris*, L Bader, J Hansen, TB Bödvarsdottir, K Coleman, P Kievit P57 Baboon Colony Housing Temperatures and Cooling Practices AD Hicks* P58 Refinement of Intratracheal Instillation Methods with a Focus on SARS-CoV-2 Animal Models AR Whigham* P59 Modification of Anesthesia Protocols to Minimize Weight Loss in Hamsters O Rodriguez* P60 Optimizing Virus Titers in Bronchoalveolar Lavage Sample Collection S Hall-Ursone* P61 Digital Animal ID as a Refinement under 3Rs EE Arlund*

CLINICAL POSTERS P100 Using a 14-day Flash Glucose Monitoring System as a Clinical Management Tool for CageHoused, Diabetic Nonhuman Primates E Jackson*, E Mitchell, K Black, V Collins, N McInnis P101 A Novel Treatment for Rodent Rectal Prolapse A Bland, F Moore, T Heather, H Pisharath, A Funk* P102 Increased Incidence of Corneal Ulcers in 5xFAD Mice D Lovell, M Hendren, S Rankin, H Pisharath, T Heather, A Funk*

P54 Development of a Temperament Testing Matrix for the Rehoming of Laboratory Rodents C Mottley*

P103 Intrahepatic Cholangiocarcinoma in a Hamadryas Baboon (Papio hamadryas) C Sella Paranzini*, MG Tamimi, K Rivas-Wagner, A Ellis, PR Morales

P55 Orotracheal Intubation: Didactic, Simulated, and Comparative Clinical Approaches in a Pig Animal Model EF Tena-Betancourt*, L Reipen, MA Resendiz Espinosa de los Monteros, A Zúñiga-Muñoz, C Riestra- Ampudia

P104 Three Dog Night: Managing 28-Hour Survival Anesthesia in Canines A Brown*, J McGrath, C Mitchell, GW Salyards, M Niekrasz

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POSTERS

P105 Lipid Bound Extended-release Buprenorphine Effectively Attenuates Postoperative Hypersensitivity in an Incisional Pain Model in Mice (Mus musculus) K Navarro*, K Jampachaisri, M Huss, C Pacharinsak

P115 Unilateral Renal Nephroblastoma with Ovarian Adhesion and Peritoneal Metastasis in an Adult Female Wister Rat OL Franco Mahecha*, S Carrasco, HR Holcombe, JG Fox, S Muthupalani

P106 Peripheral Blood Vessel Diameter and Systolic Blood Velocity in the Yorkshire Swine Animal Model: A Report of Normals Data for Size and Age S Gabriel*, WA Spinks, B Bellamy, K Szymczyk, CZ Cannon

P116 Hot Bead Sterilization of Rodent Surgical Instruments J Holdridge*, M WHEATON, W Dupont, CP Jones, K Shuster

P107 Mycobacteriosis in an Adult Zebra Finch (Taeniopygia guttata) SJ Mangosing*, S Monette, C Echeverri, LL Diaz P108 Exercise and the Overweight Research Beagle: Will it Succeed when a Controlled-calorie Diet Has Failed?

S Tyhuis* P109 Histiocytic Sarcoma with Bone Marrow Involvement in a Naïve CD-1 Mouse G Mendoza*, S Kelly, A Michel, LL Diaz P110 Assessment of a Novel Procedure to Reverse Mouse Penile Prolapse SM Setter*, IF Choi, PW Setter, HR Goold, M Fink, L Habenicht, DL Fong, LK Jori, CA Manuel P111 Necrotizing Fasciitis of the External Genitalia in a Male Rhesus Macaque (Macaca mulatta) RE Cooper*, SM Birch, MS Vermillion P112 Evaluation of Therapeutic Interventions for Rectal Prolapse in Mice Leads to Reduction in Early Euthanasia CM Mitchell*, GW Salyards, BR Theriault, G Langan, KR Luchins P113 Perioperative Care in an Adult Male Capybara (Hydrochoerus hydrochaeris) KR Strait*, J Amilcar P114 Burkholderia gladioli Infection in an Immunocompromised Rat Strain BT Adedeji*, F Noto, S Moody, C Dunn, E Baldwin, D Scott, J Stanley, H Stills

P117 Rectal Prolapse in a Female Cotton Rat (Sigmodon hispidus) TA Collins*, A Sparks, D Coble P118 Nasal Dermatitis in Small Ruminants E Houston*, J Helbling, M Burton, AR Personett, LV Kendall, JD Ayers P119 Unexplained Abdominal Distension in an Aged C3HeB/FeJ Mouse J Walton*, J Eggenschwiler, MA McCrackin, SB Harvey, U Blas-Machado P120 Transient Hyperlipidemia in a Closed Specific-Pathogen Free Cat Colony (Felis catus) M Burton*, E Houston, AR Personett, J Helbling, JD Ayers P121 Comedocarcinoma with Severe Skin Ulcerations: A Unique Presentation in a Retired Sled Dog J Draper* P122 Metastatic Hemangiosarcoma in a Young Lewis Rat Ä Sipos*, J Brazzell, JA Scholz P123 A Well-differentiated Hepatocellular Carcinoma with Pale Cytoplasmic Bodies in a Common Marmoset (Callithrix jacchus) M Stair*, JL Haupt, JG Fox, S Muthupalani P124 Branchiobdellidans Parasitization of a Laboratory Crayfish Colony D Savson*, EK Daugherity P125 Unexpected Tumor Regression in Multiple FVB/N Mice following Orthotopic Inoculation with HER2-positive Mammary Gland Tumors JA Herrod*, K Mulka, K Gabrielson, P Korangath, R Ivkov, JS Villano

P126 Continuous Glucose Monitoring in the Diabet ic Ossabaw Pig: Sensor Placement M McIntyre*, GE Geist

P128 Idiopathic Bone Disease in a Common Marmoset (Callithrix jacchus) J Brockhurst*, C Peterson, S Beck, JM Izzi P129 Refinement of Standardized Care of Ocular Lesions in Mice C Hunter*, A Skorupski, J Lawrence, PS Allen P130 The Effects of Increased Therapeutic Doses (20 Mg/kg) Of Meloxciam and Carprofen on the Welfare of Laboratory Mice AL Bailey, B Singh, BJ Smith, W McGee, LV Kendall* P131 A Novel Presentation of Mouse Urologic Syndrome In Lpgat1 +/- Male Mice M Klores*, S Rasmussen, S Peneyra P132 Reducing Stress in Rabbits during Multiple Noninvasive Ocular Imaging Procedures E Tolley*, M MacMillan, M Ortega, K Krueger P133 Isolation of Jeotgalicoccus spp. from the Tail Lesion of a Laboratory Mouse TE Whiteside*, MD Johnston, M Cesta, DM Kurtz P134 Degenerative Myelopathy Secondary to Aberrant Engraftment of Intracranially Injected, Human-origin Induced Pluripotent Stem Cell (ipsc)-derived Microglia In Nsg-csf1 Mice D Annamalai*, S Carrasco, OL Franco Mahecha, S Maddur Ganesan, A Aguilar, JG Fox, S Muthupalani P135 Atypical Clinical Presentation of Ferrets (Mustela putorius furo): Same, Same, but Different V Mrotz* P136 Spontaneous Basal Cell Tumor in a Laboratory Rat (Rattus norvegicus) C Si*

P137 Whonet Surveillance Facilitates the Identification of Multidrug Resistant Pathogens, Including Meropenem Nonsusceptible Enterobacteriaceae from Macaque Cephalic Implants A Garcia*, EM Buckley-Jordan, JG Fox

P138 Pregnancy-associated Thrombocytopenia and Bone Lesions in a Common Marmoset (Callithrix jacchus) S Maddur Ganesan*, OL Franco Mahecha, S Muthupalani, JL Haupt, JG Fox P139 Sustained Release Buprenorphine Effectively Attenuates Thermal Hypersensitivity in an Incisional Model of Postoperative Pain in Neonatal Rats (Rattus norvegicus) A Blaney* P140 Bovine Corona Virus as a Differential for Respiratory Disease in a Calf AN Varley*, TL Whitcomb, H Atkins P141 The Effects of Topical Nitroglycerin Treatment on Skin Erosions in Macaca mulatta E Lambert, M Gonzalez, DE Stockinger, K Mendoza*, J Roberts, G Hwa P142 Chronic Kidney Disease in a Closed, Inbred Colony of Tree Shrews (Tupaia belangeri) A Osborne*, D Sergio, J Henry, B Samuels, R Grytz, J Foote P143 M is for Munchies: Tr Mhe Effects of Transdermal Mirtazapine on Hyporexia in Macaques MJ Cukrov*, K Mendoza, DE Stockinger, J Roberts, G Hwa P144 The Pharmacodynamics and Effectiveness of Insulin Glargine versus Insulin Degludec in Diabetic Rhesus Macaques (Macaca mulatta) Using a CGM Device S Puglisi*, A Mackiewicz, L Garzel, A Ardeshir, K Christe P145 A Mast Cell Tumor with Mast Cell Leukemia in a C57BL/6 Transgenic Mouse C Chang*, TL Whitcomb, H Atkins P146 Atypical Streptococcus pneumoniae Meningitis in an Adult Male Rhesus Macaque (Macaca mulatta) DJ Esterline*, S Kirejczyk, K Ethun, D Owens, W Hanson

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P127 Factors Affecting Hematologic and Serum Biochemical Parameters in Healthy Common Marmosets (Callithrix jacchus) RM Kramer*, A Sheh, SC Artim, C Toolan, MA Burns, JG Fox

POSTERS

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P147 Microbiologic and Histopathologic Survey of Conventionally Housed Mice from 2 Different Vendors C Si*, M Hoenerhoff, ZT Freeman

P148 Thoracodorsal Axial Flap Reconstruction in a Rhesus Macaque AR Green*, SJ Copeland, K Mendoza, D Stockinger, J Roberts, G Hwa P149 Diagnosis and Management of Tracheal Trauma in 2 Rhesus Macaques (Macaca mulatta) SM Kezar*, M Shroyer, K Russell-Lodrigue, S Bohm P150 Trueperella pyogenes-associated Surgical Site Infections in Dorset Sheep (Ovis aries) M McKinney*, KR Finnie, R Ukita, JR Talackine, C Pinelli, M Bacchetta, CP Jones P151 Abdominal Mass in a Female Wistar Rat LT Ferguson*, L Birdwell, U Blas-Machado, VK Lee

HUSBANDRY/MANAGEMENT POSTERS P201 Low-cost Alternatives for Group Housing Rabbits on the Floor T Humbird* P202 Effectiveness of Hybrid Hydrogen Peroxide in Decontamination of Isolators, Their Contents, and Their Filters FM Grinstead*, D Holland, L Watkins P203 Edible Nail Polish as a Novel Enrichment Strategy for Singly Housed Nonhuman Primates J Martin, E Liebl, W Williams* P204 Where Do You Place Your Hydrogel?

J Locklear* P205 Husbandry and Management of Breeding Colonies of Jamaican Fruit Bats (Artibeus jamaicensis) for use in SARS-CoV-2 Research E Houston*, C Lovelace, T Schountz, LV Kendall, JD Ayers

P206 Assessment of Opaque Tubing Enrichment to Reduce Stereotypic Behaviors and Promote Breeding Efficiency in Gerbils (Meriones unguiculatus) L Habenicht*, S Bozan, BM Clancy, CA Manuel, DL Fong, JK Leszczynski, A Klug P207 The Genetic Diversity of Diversity Outbred Mice H He, V Philip, KL Brackett* P208 Individual Tracking of LPS- or Chlorpromazine-induced Changes in Locomotion of Pair-housed Mice in Smart Cages J Ramirez*, E Defensor, C Liu, LR Schaevitz, JE Fenyk-Melody P209 Introducing and Troubleshooting a Sealed Positive-pressure Rack System to House Germfee Mice J Yamada, E Chua, K McEachin, R Garcia-Gonzalez*, C Sohn P210 Accessible Caging for Mice with Partial Paralysis PB Hutchison*, NL Herndon P211 Is More Really Better? A Reevaluation of PPE Practices in a Barrier Rodent Facility TA Collins*, CL Freed, E Brooks P212 Handling and Husbandry Technique Adaptations Involved in the Assessment, Blood Drawing, Euthanasia, and Dissection Of Juvenile Wild-caught Alligators RK Banks* P213 Establishment of a Shared Database to Facilitate a Reduction of Animal Use at a Large Academic Institution A Meyer*, DL Hickman P214 Operational Excellence, Risk Management, and Compliance Supports the Integration of an Insectary into an Established Research Vivarium C Mateo, L Magee*, J Keding-Casey, M Fishbaugher, C Osborne P215 Tox in a Box: Enhancement of a Closed Restraint Chair for Laboratory Macaques CE Ferrecchia*, LF Matney

P217 Evaluation and Implementation of Bagged, Crinkle-cut Paper Strips as Environmental Enrichment for Mice JC Rodgers*, C Cheleuitte, NS Lipman P218 Influence of Feeding Frequency and Brine Shrimp (Artemia salina) on the Growth on Zebrafish (Danio rerio) R Giallella, D Garrity, P Robison, J Owiny* P219 Rearing and Vaccination of the Neonatal Muntjac Deer (Muntiacus reevesi) J Helbling*, E Houston, M Burton, AR Personett, E McNulty, C Mathiason, JD Ayers, LV Kendall P220 Norovirus No More: The Power of Dry Heat Sterilization to Stop the Transmission of Murine Norovirus in Dirty Bedding J Lee*, WA Bidot, EA Nunamaker P222 Ammonia Levels in Rat Double Decker Individually Ventilated Cage System P Kowal*, m Defeo, M Mooney

P227 When to Wean? A Visual Guide to Black, White, and Agouti Mouse Pup Growth for Postnatal Days 14-28 SM Kirchain*, E Brogan P229 How a New and Unique Digital Platform Improves Efficiency, Tracking, and Access to Information in the Research Vivarium EE Arlund* P230 Let it Shine: Incorporating Innovative Design Concepts to Improve Nonhuman Primate Wellbeing L Singer*, P Kowal, ST Baker P231 Development and Standardization of a 72-h Swine Prolonged Field Care Model Using Supplies Available to U.S. Department of Defense Laboratories GA Pratt*, L Rivera-Lopez, AR Martinez, MM Dennis, MA de la Garza, K Rapp-Santos, MM Tiller, JJ Glaser, ND McNeal P232 Peromyscus: the Wild Mouse of the Laboratory Animal World L Ward*, R Kilby, A Narizzano P233 Personnel Protective Equipment Management at a Large Academic Facility during COVID-19 Supply Chain Disruption NH Shomer*, J Rohr

P223 Is Your Disaster Plan Ready for Post-pandemic Normal?

J Brooks*, S Kirchain, R Thompson, G Cronin, J Nichol, P Holly

P234 A Novel Tool for Recordkeeping at Cage Side in a GLP Environment G Stodieck*, A Corcoran, A Craig

P224 Finding an Alternative Approach when Critical Medical Supplies Are in Short Supply during the COVID-19 Pandemic RA Byrum*, MC St Claire

P235 Interspecies Cohabitation of Agricultural Laboratory Animal Species as a Means for Social Housing DM Nguyen*, Z Graham, DE Collins

P225 The Effect of Environmental Enrichment on Stress, Behavior, and Production in Gestating and Farrowing Sus scrofa Sows C Peck*, J Kieffer, S Moeller

P236 Impact of Compressed Paper Bedding on Breeding Performance and Recognition of Animal Health Concerns in Mus musculus PS Allen, J Lawrence, U Stasula, D Keys, B Pallas, ZT Freeman*

P226 The Cryodropper: A Novel Device for Mouse Embryo and Sperm Vitrification B Stone*

P237 Increasing Institutional Engagement for the Reduction of the Number of Discarded Animals Using the Lean Six Sigma Methodology FP Paiva*, BP Paiva, IA Freire

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P216 Assessment of Existing and Novel Tissue Sample Collection Methods for Standard and Automated Rodent Genotyping RC Dysko*, FC Hankenson, F Benavides, LA Colby, PC Smith, DK Taylor, N Nowak, DE Hoskins

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P238 A Retrospective Review of Aggression-related Injuries in a Colony of Alston’s Singing Mice (Scotinomys teguina) R Tierce*, M Tran, S Peneyra, S Rasmussen

P306 Hepatocellular RECK Overexpression Attenuates Nonalcoholic Steatohepatitis Susceptibility R Dashek*, R Cunningham, M Moore, V Jepkemoi, G Meers, B Chandrasekar, R Rector

P239 Calculating Hub Loss of Controlled Substances Using Specific Gravity S King, B Skiles, DL Hickman*

P307 The Effects of a Decreased Light Adaptation Time on Electroretinogram (ERG) Results in Nonhuman Primates KJ DePlancke*, R Boyd

P240 Disaster Planning in the Midst of a Pandemic K Lux*, J Escobedo, K Hendrix P241 Creating a Macaque Nursery J Roberts*

LABORATORY INVESTIGATIONS POSTERS P300 Serum Pharmacokinetics of a Highly Concentrated Buprenorphine Formulation in Female Sprague Dawley Rats (Rattus norvegicus) K Galang*, H Knych, R Oates P301 Development of a Noninvasive Transdermal Technique for Serial CSF Collection from Cisterna Magna In Rats A Fiorenza*, P Lopez, H Masakazu P302 Comparison of Two Isolation Techniques for Macaca fascicularis Peripheral Blood Mononuclear Cells: Cell Recovery, Cell Viability, and Purity DR Rodriguez* P303 Age-related Changes in GFAP-Immunoreactive Astrocytes in the Mouse Cerebellar Molecular Cell Layer C Tyszkiewicz*, ID Pardo, C Liu, CJ Somps P304 The Relationship of Body Temperature to Noise-induced Hearing Loss C Yancey*, M West, W Williams P305 Longitudinal Characterization of Ladder-climbing Behavior in Young Duchenne Muscular Dystrophy MDX Mice: A Smart Cage Study S Hwang*, C Liu, J Pang, M Montgomery, B Manickam, H Zhang, LR Schaevitz, R Goldstein

P308 Chronic Systemic Injection of DREADDs Agonists Clozapine-n-oxide and Compound 21 Does not Change Behavior Relevant to Locomotion, Exploration, Anxiety, or Affect in Male Mice SL Spears*, F Tran, K Ahn, AJ Eisch, S Yun P309 Isoflavone Intake Causes Adrenal Dysfunction Resulting in the Delayed Puberty In Prepubertal Male Rats S Caceres*, A Alonso-Diez, B Crespo, G Silvan, M Illera, PC Carlos, D Inmaculada, JC Illera P310 Reduction of Corynebacterium bovis Viability following Freeze-thaw in Tumor Cryopreservation Media G Mendoza*, RJ Ricart Arbona, K Lertpiriyapong P311 Impact of Thermal Support Systems on the Physiologic and Recovery Parameters of Anesthetized Common Marmosets (Callithrix jacchus) AL Cates, JL Haupt*, JG Fox P312 Evaluation of Hot Bead Sterilizers Used for Rodent Surgery JL Haupt*, H Nam, S Lackeyram-Owen, RM Kramer, JG Fox P313 Efficacy of a Commercial Primate Diet Containing Fenbendazole in Treating Trichuris trichiura Infection in a Captive Baboon Colony (Papio sp.)

JW Dutton*, LL Condel, JB Jimenez, SE Price, DB Elmore P314 Evaluation of Topical Treatment Regimens for Ulcerative Dermatitis in C57BL/6J Mice BL Kick*, B Lyons, R Doty P315 Size of Cage Used for Euthanasia Can Affect the Welfare of Rats During Euthanasia DL Hickman*

P318 The Impact of CO2 EuthanRsia on Sperm Quality, In Vitro Fertilization and Embryo Development Tates S Gerb*, L Stevey, C Agca, Y Agca P319 Functional Analysis of Novel Genetic Factor for Chronic Kidney Disease in Mice GLAS: Yes Y Takahashi*, H Sasaki, K Hiura, M Watanabe, K Nakano, T okamura, N Sasaki

P326 Comparing the Effects of Irradiation and Mammary Fat Pad Implantation on Patient-derived Xenograft Model Characteristics T Chan*, D VanBuskirk, R Banzon, M Cheng, K Draheim P327 Characterizing the Pathogenesis of Murine Chapparvovirus in CD-1, C57BL/6, and NSG Mice ML Kain*, S Monette, RJ Ricart Arbona, NS Lipman P328 Efficacy of Beta-defensin Derived Cationic Antimicrobial Peptides against Biofilm-producing Bacteria E Knebel*, Z Shen, S Zhang P329 Isoflurane and Carbon Dioxide Elicit Similar Behavioral Responses in Rats S Kulkarni, DL Hickman*

P320 Tensin 2 Suppresses Intestinal Tumor Progression via Inhibiting the Wnt/β-catenin Pathway K Hiura*, Y Takahashi, M Watanabe, K Nakano, T okamura, S Kakuta, H Sasaki, N Sasaki

P330 Location of Gas Inlet Can Negatively Affect the Well-being of Rats Euthanized with CO2 DL Hickman*

P321 A Unique Uncoupling of Cell Death and IL-1 Release in a Mouse Model of Meningitic Escherichia coli K1 Infection C Chambers*, JA Skyberg

P331 Biocompatibility Testing of a Novel Cranial Implant in a Rhesus Macaque J Schoenberger*, D Mailhiot, N Pach, KR Luchins, M Niekrasz

P322 Age- and Sex-related Changes in Hematologic and Biochemical Parameters of Dunkin Hartley Guinea Pigs AR Personett*, MF Afzali, SB Bork, LH Burton, LB Radakovich, CA Seebart, KS Santangelo

P332 Investigation of the Role of Il-22 In Helicobacter pylori-induced Chronic Gastritis in Murine Models M Stair*, E Bryant, HR Holcombe, S Muthupalani, Z Ge, Z Shen, D Puglisi, A Aguilar, JG Fox

P323 Effect of Social Housing on Changes in Monocyte Expression in Pigtail Macaques (Macaca nemestrina) during Acute Simian Immunodeficiency Virus Infection N Castell*, S Guerrero-Martin, E Shirk, J Brockhurst, S Queen, M Li, B Bullock, B Carlson, R Adams, L Gama, D Graham, C Zink, J Clements, J Mankowski, KA Metcalf Pate

P333 Coagulation Parameter Reference Intervals for Inbred Strain 13/n Guinea Pigs (Cavia Porcellus) and Validation of Low Volume PT and aPTT Sample Analysis JA Condrey*, T Flietstra, K Nestor, E Schlosser, J Coleman-McCray, S Genzer, S Welch, J Spengler

P324 Effects of Oxygen Supplementation on Ketamine/Xylazine Anesthesia in C57BL/6 Mice CE Blevins*, NA Celeste, J Marx P325 Use of Entyce in New Zealand White Rabbits (Oryctolagus cuniculus) J Draper*, EK Daugherity

P334 Cannabidiol and Cannabidiolic Acid-rich Hemp Oil for the Treatment of Diarrheal Disease in Cynomolgus Macaques (Macaca fascicularis) TN Johns*, AI Cameron, WM Burnside P335 Inhibition of Fibroblast Activation Rescues Cardiac Dysfunction Associated with Inherited Dilated Cardiomyopathy KA Zabrecky*, K Kooiker, D Bugg, A Martinson, J Davis

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P316 Evaluation of Animal Welfare of Socially Housed Aged Male Sprague Dawley Rats B Skiles, S Kulkarni, DL Hickman* P317 Comparison of Gut Microbiota from 2 Different Colonies of Guinea Pigs D Chadalavada*, P Subramanian, J Perez-Chaparro

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P336 The Superfund Chemical, N-nitrosodimethylamine, Induces an Early Onset Pattern of Mutations AL Armijo*, S Chawanthayatham, BI Fedeles, RG Croy, JG Fox, JM Essigmann P337 Prevention of Corneal Opacities during Ocular Imaging in Rats HB Culp*, M Ault, X Ping, L Yang, C Nunes, J Destefano, L Gichuru, L Chen, I Petrescu, C Johnson, L Handt, S Bellum, N Li, S Motzel, X Shen P338 Impact of Social Housing on Long-term Patency of Jugular Catheters in Rats (Rattus norvegicus) L Krueger*, SE Chang, M Motoc, M Chojecki, ZT Freeman, SB Flagel P339 Effects of Isoflurane on Engraftment Rates and Cytokine Release In Humanized Mice M Harris*, m chen, J Yang, G Yang

P340 Fecal Microbiome Monitoring in Colony and Sentinel Mice PC Smith, J Gu, C Zhou, CJ Zeiss, S Compton, PA Cirillo, H Wallace, J Macy*

P346 Lessons Learned during Murine Microbiome Analysis on a Decentralized Campus S Hashway*, J Reuter P347 Improving the Welfare of Hamsters during Euthanasia DL Hickman* P348 Assessment of Indicators of Well-being In Laboratory Rats and Mice Euthanized with Carbon Monoxide DL Hickman* P349 Evaluation of How Social Interactions Influence the Welfare of Rats during Euthanasia DL Hickman* P350 Assessing the Welfare of Mice following Repeat Administration of Anesthetic Agents B Skiles*, T Shafer, S King, DL Hickman P351 A Diet-induced Model of Nonalcoholic Steatohepatitis in Aged Male and Female C57BL/6J Mice J Serrano, R French, K Leighton, D Imai, A Schile*

P341 Optimization of Superovulation Hormone Injection Intervals JA Cayton*, C Bethune, C Piotter, J Walls, D Davis, H Men, CL Franklin

P352 Unilateral Vagotomy Alters Astrocyte Morphology in the Nucleus Tractus Solitarii of the Brainstem GC Hofmann*, EM Hasser, DD Kline

P342 Postsurgical Recovery Assessment of Mice Treated with Extended- or Sustained-Release Buprenorphine M Saenz*, E Bloom-Saldana, PT Fueger, R Ermel, J Finlay

P353 Detection of Early Myocardial Cell Death in Owl Monkeys Using Complement Component C9 Immunohistochemistry in Formalin-fixed Paraffin-embedded Heart Tissues: A Retrospective Study A Gozalo*, L Lambert, W Elkins

P343 Reducing Inter-operator Variability Using a Novel 3D and Thermal Measurement System when Measuring Subcutaneous Tumors in Mice A Smith, K Turley, J Steed*

P354 High-fat Diet and Age Are Associated with Skin Mast Cell Degranulation in C57bl/6j Female Mice: A Possible Role Iin Ulcerative Dermatitis A Gozalo*, J Qin, M Akkaya, M PeĂąa, W Elkins

P344 Inactivation of Amplicons Using Gaseous Chlorine Dioxide K Lorcheim*

P355 Effect of Social Stress On Cd8+ T Cell Responses during Chronic SIV Infection in Antiretroviral Therapy-treated Pigtail Macaques GLAS: Yes S Diaz*, S Guerrero-Martin, E Shirk, S Queen, M Li, B Bullock, B Carlson, R Adams, L Gama, D Graham, C Zink, J Clements, J Mankowski, KA Metcalf Pate

P345 Zebrafish (Danio rerio)Â Embryo Surface Disinfection: Refining the Bleaching Protocol GLAS: Yes A Winn*, K Prestia, S Peneyra

P357 Assessment of Welfare of Female Sprague Dawley Rats Euthanized with a Commercially Available System DL Hickman* P358 Investigation of SARS-CoV-2 Spike Protein as a Vaccine Target and Development of COVID-19 Companion Diagnostics for Use in Nonhuman Primates L Moser, K Mansfield, MA Burns, L Martin, D Cunha, J Fischer, D Rancour, F Schomburg, K Luke* P359 Protective Effect of Molecular Hydrogen on Oxidative Stress-induced Impairment in Mouse Sperm Mitochondria Dysfunction Y Noda*, T Nemoto, T Endo, I Ohsawa

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P356 Analysis of the Transgene Insertion Pattern in a Transgenic Mouse Strain Using a Long-read Sequencer O Suzuki*, M Koura, K Uchio-Yamada, M Sasaki

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