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Ramos [RA 1] Cell Line Catalog No.: EP-CL-0483
Origin and General Characteristics Cell Name
Ramos [RA 1] Cell Line
Organism
Homo sapiens, Human
Age
3 years
Tissue
Peripheral blood
Morphology
Lymphoblast
Growth Properties
Suspension
Descriptions
The cells are negative for Epstein-Barr virus. Both the parental line and the derivative cell line Ramos. 2G6. 4C10 have about 1500 IL-4 binding sites per cell. The cells are reported to secrete IgM(lambda light chain).
Biosafety Level
1
Culture Conditions and Handling Complete Growth Medium
RPMI-1640 +10% FBS +1% P/S
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
Subcultivation Ratio
1:2-1:4
Medium Renewal
Every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Special Features of the Cell Line Tumorigenic
Yes
Effects
Yes, forms tumors in nude mice.
Receptor Expression
Interleukin 4(IL-4); low affinity IgE(CD23)
Antigen Expression
CD23＋
Gene Expression
Immunoglobulin(Surface And Secreted)
Applications
Transfection.
Recommendations for handling of cryopreserved cells 1.
The cell is packaged by dry ice. When receiving the cell, please make sure that the vial is still frozen. If there is cell thawing in the tube, please take photo before experiment or storage.
2.
If immediate culturing is not intended, the cryovial(s) must be stored in liquid nitrogen (-196°C) or at least at -80°C after arrival. If immediate culturing is intended, please follow these instructions:
3.
Quickly thaw by rapid agitation in a 37℃ water bath within 45-90 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water
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