Tel:240-252-7368(USA) Fax:240-252-7376(USA) www.elabscience.com E-mail:techsupport@elabscience.com Elabscience Biotechnology Inc.
Elabscience® SK-N-BE(2) Cell Line Catalog No.: EP-CL-0441
Origin and General Characteristics Cell Name
SK-N-BE(2) Cell Line
Organism
Homo sapiens, Human
Age
2 years
Tissue
Brain, derived from metastatic site: bone marrow
Morphology
Neuroblast
Growth Properties
Mixed, Adherent And Suspension
Descriptions
The cells exhibit moderate levels of dopamine beta hydroxylase activity.SK-NBE(2) cells have a reported saturation density greater than 1×10^6 cells/cm^2.The morphology of the cells varies with some cells having long processes and others that are epithelioid like.The cells will aggregate, form clumps and float.
Biosafety Level
1
Culture Conditions and Handling Complete Growth Medium
MEM/F12+10% FBS +1% P/S
Subculturing
Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio
1:2-1:4
Medium Renewal
Every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Special Features of the Cell Line Tumorigenic
Yes
Effects
Yes, an inoculum of 10^7 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency.
Recommendations for handling of cryopreserved cells 1.
The cell is packaged by dry ice. When receiving the cell, please make sure that the vial is still frozen. If there is cell thawing in the tube, please take photo before experiment or storage.
2.
If immediate culturing is not intended, the cryovial(s) must be stored in liquid nitrogen (-196°C) or at least at -80°C after arrival. If immediate culturing is intended, please follow these instructions:
For Research Use Only Thank you for your recent purchase. If you would like to learn more about proteins,please visit www.elabscience.com.
Focus on your research Service for life science