Elabscience NCI-H358 Cell Line Manual

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NCI-H358 Cell Line Catalog No.: EP-CL-0400

Origin and General Characteristics Cell Name

NCI-H358 Cell Line

Organism

Homo sapiens, Human

Tissue

Lung/bronchiole, derived from metastatic site: alveolus

Morphology

Epithelial

Growth Properties

Adherent

Descriptions

Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.

Biosafety Level

1

Culture Conditions and Handling Complete Growth Medium

RPMI-1640 +10% FBS +1% P/S

Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Subcultivation Ratio

1:2-1:4

Medium Renewal

Every 2 to 3 days

Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Special Features of the Cell Line Tumorigenic

Yes

Effects

Yes, the cells produce tumors in athymic nude mice.

Gene Expression

Lung Surfactant Associated Protein A(Sp-A), The Cells Expressed Protein And Rna Of Sp-A, The Major Lung Surfactant Associated Protein.

Applications

This line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Cla

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