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NCI-H358 Cell Line Catalog No.: EP-CL-0400
Origin and General Characteristics Cell Name
NCI-H358 Cell Line
Organism
Homo sapiens, Human
Tissue
Lung/bronchiole, derived from metastatic site: alveolus
Morphology
Epithelial
Growth Properties
Adherent
Descriptions
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.
Biosafety Level
1
Culture Conditions and Handling Complete Growth Medium
RPMI-1640 +10% FBS +1% P/S
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio
1:2-1:4
Medium Renewal
Every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Special Features of the Cell Line Tumorigenic
Yes
Effects
Yes, the cells produce tumors in athymic nude mice.
Gene Expression
Lung Surfactant Associated Protein A(Sp-A), The Cells Expressed Protein And Rna Of Sp-A, The Major Lung Surfactant Associated Protein.
Applications
This line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Cla
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