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SW480 [SW-480] Cell Line Catalog No.: EP-CL-0223
Origin and General Characteristics Cell Name
SW480 [SW-480] Cell Line
Organism
Homo sapiens, Human
Age
50 years
Tissue
Colon
Morphology
Epithelial
Growth Properties
Adherent
Descriptions
A cell line established from a lymph node metastasis taken from the same patient one year later is available.The line is negative for CSAp(CSAp-) and colon antigen 3.The cells are positive for keratin by immunoperoxidase staining.There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution and a C-】 T mutation in codon 309 resulting in a Pro-】 Ser substitution.The cells express elevated levels of p53 protein.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.N-myc oncogene expression was not detected.Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed.The cells have been reported to produce GM-CSF.
Biosafety Level
1
Culture Conditions and Handling Complete Growth Medium
DMEM +10% FBS +1% P/S
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio
1:2-1:4
Medium Renewal
Every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Special Features of the Cell Line Tumorigenic
Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
Effects
Yes, in nude mice.
Receptor Expression
Epidermal growth factor(EGF)
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