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JeKo-1 Cell Line Catalog No.: EP-CL-0128
Origin and General Characteristics Cell Name
JeKo-1 Cell Line
Organism
Homo sapiens, Human
Age
78 years
Tissue
Peripheral blood, mantle cell lymphoma
Morphology
Lymphoblast
Growth Properties
Suspension
Descriptions
The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. Ref JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.
Biosafety Level
1
Culture Conditions and Handling Complete Growth Medium
RPMI-1640 +20% FBS +1% P/S
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Subcultivation Ratio
1:2-1:4
Medium Renewal
Every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Special Features of the Cell Line Antigen Expression
CD3-, CD5＋, CD10＋, CD19＋
Gene Expression
Cd3-, Cd5＋, Cd10＋, Cd19＋
Applications
JeKo-1 cells are highly tumorigenic in SCID mice.
Recommendations for handling of cryopreserved cells 1.
The cell is packaged by dry ice. When receiving the cell, please make sure that the vial is still frozen. If there is cell thawing in the tube, please take photo before experiment or storage.
2.
If immediate culturing is not intended, the cryovial(s) must be stored in liquid nitrogen (-196°C) or at least at -80°C after arrival. If immediate culturing is intended, please follow these instructions:
3.
Quickly thaw by rapid agitation in a 37℃ water bath within 45-90 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath.
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