Biotecnika & Rasayanika
Curriculum for CRISPR Bioinformatics Internship: Learn to Edit Genome
14-Days: Hands-on Internship with Lab Protocols
Session No.
Unit No. And Topic
MODULE-1: CRISPR Foundation
Day 1 Scope & Opportunities in Genome Editing Technology: India & Abroad
Day 2 Basics of CRISPR System and related Terminology
Day 3 Ethical responsibilities and limitations of CRISPR Technology: Are we Ready?
MODULE-2: Understanding CRISPR/Cas Biology & their Assembly
Day 4 Classification of CRISPR/Cas System and their function
Day 5 Principles of CRISPR/Cas in molecular diagnostics
Day 6 PCR and Real-time PCR platform in CRISPR Validation & Interpretation
Day 7 ELISA and Fluorimetry: Alternatives for CRISPR Validation & Interpretation
MODULE-3: BioSoft Tools Used in CRISPR Technology
Day 8 Primer V3, BE-Designer, CRISPR direct, CRISPR Design tool, CRISPR-P 2.0
Day 9 CRISPR Target, CRISPR finder, E-CRISP, CRISPR optimal target finder
Day 10 CHOP-CHOP, CRISPy Web, Cas Designer, CRISPR scan, CRISPR Library Designer
Day 11 IDT Design custom gRNA, Synthego CRISPR guide design, CRISPR-Focus, CRISPR MultiTargeter
MODULE-4: CRISPR-Wet lab experimental designwithProtocols
Day 12
CRISPR - Biochemical concentrations and calculations
Day 13 Case study: CRISPR/Cas-based diagnosis of Ehrlichia canis infection in dogs
Day 14 Implications of CRISPR in Microbial, Plant, Animal, and Humans models for detection, diagnosis, and therapy.