Cytogenetics Molecular Cytogenetic Analysis for Acute Lymphoblastic Leukemia Test Purpose
: To diagnose and monitor the treatment response of patients with B-cell acute lymphoblastic leukemia (B-ALL).
Methodology : Fluorescence In Situ Hybridization (FISH). Testing Procedure
: This test includes culture of blood / bone marrow cells, followed by analysis of special sites of the chromosome using fluorescent probes.
Gene Analyzed
: BCR-ABL1, t(9:22)(q34;q11).
Turn Around Time
: 4 working days.
Specimen Type
: Bone marrow (Preferably)/ Peripheral blood.
Specimen Volume : 3-4 ml bone marrow / 4 ml blood in green top heparin tube. Collection instructions : Label the vacutainer with patient’s name and specimen type. Specimen Transport : The specimen should be shipped at 18-25°C, do not freeze. It should reach the lab within 24 hours of collection (preferably at the earliest). In case of delay, sample to be stored on the door shelf of the refrigerator. Mandatory Complete blood count and bone marrow/ peripheral blood blast percentage must be provided. Requirements : Specify detailed clinical history, collection date & time of sample withdrawn on test request form*. Significance of Gene tested
: BCR-ABL1, t(9:22)(q34;q11) ¤ Present in 25% adult B-ALL and 2-4% Pediatric B-ALL cases. ¤ Results in chimeric fusion protein with constitutive tyrosine kinase activity. ¤ Presence of BCR-ABL1 fusion gene confers a poor prognosis.