24 Issue 3 2020
ion chromatography life sciences
No rocket science – maximising the benefits of Ion Chromatography Mass Spectrometry
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C-MS is a proven method for the highly sensitive and selective analysis of anions, oxoanions, herbicides and pesticides. Metrohm enhances the benefits of this method by integrating inline sample preparation for more convenience, higher throughput and better reproducibility of results. A very useful technical poster gives an overview of the many possible applications. You can download it by putting this link in your browser: https://bit.ly/2XTBSov. The Metrohm IC-MS method is dedicated to developing selective, sensitive, robust and accelerated applications for the detection of ions in matrix-loaded samples (e.g. soil, foodstuffs, biomass, fertilisers, polymers, salts, explosives, pharmaceuticals and plating baths). Only small sample volumes in the µL-range are required and
trace detection is possible even in the presence of interfering matrix components. Organic modifiers can be directly added to the eluent (up to 100%) to improve sample evaporation and MS-signals. For ultimate user convenience, the Metrohm IC and the Waters single quadrupoles are easily operated under the same software (Empower 3). Automated Metrohm Inline Sample Preparation (MISP) techniques (e.g. Inline Ultrafiltration, Dilution, or Dialysis) protect the column, increase reproducibility of results and minimise time required in the laboratory. The method was used to determine the following: • various small ions at trace-level concentrations such as fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulphate • glyphosate and various other herbicides and their residues • perchlorate at ultra-trace levels in various matrices • oxoanions as well as thiosulfate and thiocyanate.
Integra launches two new high-volume pipettes
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ntegra Biosciences is expanding its comprehensive collection of pipettes with the upcoming launch of two new Evolve manual pipettes – high volume 8 and 12 channel options offering a market-leading capacity of 1250µl per channel. The incredibly popular Evolve family already offers users a full assortment of single, 8, 12 and 16 channel manual pipettes, covering a volume range of 0.2 to 5000µl. These latest additions were developed in response to customer requests for large volume multichannel pipettes – especially useful for cell culture applications. The manually actuated plunger allows you to generate significantly more force for aspirating and dispensing than an electronic pipette, making them perfect for vigorous mixing and
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breaking up cell pellets. These new pipettes retain all the same beneficial features of the Evolve pipette range, including quick set dials that allow volumes to be set in the blink of an eye, and a lightweight ergonomic design for more comfortable and productive pipetting. All Evolve pipettes use Integra’s GripTip pipette tips, with the range including a number of specialty tips aimed at cellular applications, such as 1250µl short tips – for more ergonomic pipetting – and wide bore tips, which help to eliminate cell shearing and aid pipetting of viscous solutions. For higher throughput cell-based applications, Integra also offers the Viaflo 96/384 – a compact, affordable and easy to use solution for 24, 96 and 384 channel pipetting – enabling filling, mixing or seeding of an entire plate in one dispense.
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Tel: (011) 656 1918 CPT: (021) 852 0213 DBN: (031) 265 0067 Fax: (011) 656 2698 078 643 7113 info@metrohm.co.za www.metrohm.co.za
JHB: (011) 315 5434 CPT: (021) 531 7660 DBN: (031) 566 4870 Fax: (011) 315 5882 sales@labotec.co.za www.labotec.co.za
Know your ions – overcoming the limits of HPLC with ion chromatography made ion chromatography a powerful tool for both research and routine analysis. Laboratories in the pharma, food, and water sectors, in particular, benefit not only from the method itself but from many automated inline sample preparation and injection techniques. Samples with a high load of particles (e.g. waste water), solid and even gaseous samples can be analysed fully automatically down to the μg/L range with superior reproducibility. Hundreds of IC applications are available free of charge from the Metrohm Application Finder and users are supported in more than 120 countries by
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hat’s the difference between High Pressure Liquid Chromatography (HPLC) and Ion Chromatography (IC)? What are the capabilities and limitations of each technique? Knowing these differences can actually make a huge difference. As a proven, robust and highly versatile technique, IC can help HPLC users extend their analytical capabilities tremendously. Metrohm’s free white paper compares the two methods systematically, focussing on the strengths of ion chromatography. HPLC is one of the most powerful analytical techniques in the modern laboratory. From research to
manufacturing to legal, it is widely used in many sectors. However, there are some inherent limitations. Ionic and polar compounds pose a challenge. So do organic acids. Moreover, some analytes such as fluoride and chloride are not active in the UV range, making it impossible to determine them with a UV detector, the detection technique most commonly used with HPLC. Ion chromatography (IC), on the other hand, is ideal for the separation and detection of ions and polar molecules, both organic and inorganic. This makes IC the ideal extension of HPLC, increasing the analytical capabilities of the modern laboratory tremendously. Over the last 30+ years, Metrohm has
application specialists from the local Metrohm sales organisations. The white paper can be downloaded by going to this link in your browser: https://bit.ly/39AoMhT.
METROHM SA (PTY) LTD Tel: (011) 656 1918 CPT: (021) 852 0213 DBN: (031) 265 0067 Fax: (011) 656 2698 078 643 7113 info@metrohm.co.za www.metrohm.co.za
