Biomicrofluidics Core

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Texas Children’s Neurological Research Institute

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Core Director: Lidong Qin, PhD 713.441.7328 Email: lqin@houstonmethodist.org

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Biomicrofluidics Core

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Houston Methodist Research Institute

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Core Manager: Erkuan Wang 713.441.9633 Email: ewang3@houstonmethodist.org

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The Biomicrofluidics Core at Houston Methodist Research Institute is staffed by experienced professionals who are experts in biomicrofluidic chip design and fabrication. Our services are available to investigators worldwide.

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Biomicrofluidics Core

Single-cell Analysis Chip Block-Cell-Printing is a unique live-cell printing technique that positions single cells on a PS/glass substrate in a precise and controllable fashion, and enables multiplexing and high-throughput printing of functional single-cell arrays. This technique has wide application prospects in screening cell function and heterogeneity, including cell protrusion, cell-cell communication via gap junctions, and patterning of primary neurons.

M-Chip

Our characteristic pre-made microfluidic chips are: V-Chip The V-Chip can measure and display the concentration of a biomarker in a biological sample quickly, without relying on optical or electronic components. This microfluidic device is cheap and easy-to-use, allows for the measurement of up to 50 samples in parallel, and displays quantitative results in the form of intuitive ink bar graphs directly on the device.

“ The V-Chip is accurate, cheap and portable.

It requires only a drop of blood, not a vial, and can do 50 different tests in one go.”

Lidong Qin, PhD Associate Professor of Nanomedicine, Institute for Academic Medicine Associate Member, Houston Methodist Research Institute Scientist, Department of Nanomedicine Houston Methodist

• OAI model 200 Mask Aligner • Self-built UV exposure system • Nikon Eclipse LV 100ND microscope •P lasma Etch PE50 and Harrick plasma cleaners • T hermoscientific Super-Nuova single-position digital stirring hotplates • Dongda engraving machine • Schmidt Tech holes puncher

Cell Separation Chip The cell separation chip has a micropost array structure and is able to separate cells by size and deformability in a high-throughput manner. We have used this chip for probing cellular heterogeneity and identifying more invasive phenotypes in cancer cells.

Single-Cell Pipet Tip The single-cell pipet tip (SCP-tip) can transfer and deposit live single cells directly from a cell suspension to any single cell container. The tip is compatible with the commonly used air-displacement-pipette (ADP). It provides for low cost, rapid, controllable, single cell manipulation with high cell viability, high efficiency, and operational simplicity.

V-CHIP

The Houston Methodist Research Institute Biomicrofluidics Core creates custom designed microfluidic molds and chips, fabricates pre-made biomicrofluidic chips, and provides consultation services in the biomicrofluidics field.

The M-Chip is a high-throughput microfluidic device with 3120 ultra-miniaturized chambers to monitor mesenchymal-mode migration and screen for anti-metastatic drugs that specifically inhibit mesenchymal migration. The small size of samples used with the M-Chip allows detection and analysis of the metastatic potential of primary or rare cells. This device provides a high-throughput platform.

Equipment

Specialized Services The Biomicrofluidics Core offers several specialized services including consultation services for building your own microfluidic chips and fabrication of: • Custom designed replica molds used for soft lithography • Custom designed microfluidic PDMS/Glass chips • Pre-made microfluidic chips:

- V-Chip

- Single-cell analysis chip

- M-Chip

- Cell separation chip

- Single-cell pipet tip