Effects of the main mycotoxins on poultry production parameters

EFFECTS OF THE MAIN MYCOTOXINS ON POULTRY PRODUCTION PARAMETERS

Milad Manafi Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran. manafim@malayeru.ac.ir

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Chicken meat is healthy and can be produced anywhere. It is among the relatively cheapest animal-source proteins and is not subject to any type of religious restrictions (Manafi et al., 2019).

Food safety and security measures have to be taken up within the food chain from livestock feed to human food.

Producing adequate and available amount of safe feed are important factors to be considered, otherwise, it may lead to food insecurity and malnutrition (Manafi et al., 2018a).

This technical paper reviews the incidence and toxic effects of major mycotoxin in poultry.

Contamination with mycotoxins is among the hidden-potential hazards having a variety of severe adverse health impacts (Manafi

and Khosravinia, 2013).

To be more precise, mycotoxins are the secondary metabolites of different fungi (molds) species found on carbohydrate-rich feeds such as peanuts, cottonseed, corn, sorghum, and cereal grains (Manafi et al., 2014a).

There is a range of fungi that can produce different mycotoxins when put together in favorable situations of hot conditions without adequate drying and aeration (Manafi et al., 2012a).

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As FAO declared, nearly 25% of the world’s food crops

25%

crops contaminated with mycotoxins

and cereals produced annually are contaminated with mycotoxins, although this figure greatly underestimates the occurrence above the detectable levels (up to 60–80%) (Eskola et al., 2020).

This is to emphasize the importance of the world’s cereal production and distribution from two different angles: 1. The yield of production

As an expert, one should be aware of the variety and content levels of mycotoxins in the feedlot which

2. The quality of the product

they receive. Once known, farm nutritionists may think about how to

Aflatoxin contamination of feedstuffs has been

minimize those adverse effects.

reported to be of a wide range from 1 to 900μg/kg in commonly used ingredients as well as mixed feed samples in developing countries (Mohanamba et al., 2007).

It is believed that, apart from acute Different countries have set down their permissible standard levels for importing and or providing the raw materials to feed their farm animals.

poisoning and severe liver damages and lesions, these metabolites could be a cause to increase the immune deficiencies and cancer risk in livestock and subsequently be carried on to humankind, leading to

This is crucial to know, as mycotoxin

genetic mutations when available in food

contamination starts right from the

for a long-run (Manafi et al., 2009).

production phase and is continued during harvest, transportation, and

When comes to poultry, currently aflatoxins,

storage, before reaching the final

ochratoxins, and T-2 toxins are considered

customer in every corner of the globe.

to be the most dangerous mycotoxins

Developing countries have very strict rules when it comes to this matter, but in certain countries, especially in Africa, a shortage

(Table 1) from food safety and regulatory viewpoints, as well as negatively affecting poultry production parameters (Eskola et al., 2018).

of raw material supply is affecting the permissible level (EUR-Lex., 2021).

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Table 1. Relative toxicity of different mycotoxins on different livestock species Toxin

Poultry

Ruminants

Swine

Aflatoxins

+++

+

++

T-2 toxins

++

+++

+++

Ochratoxin

+++

+

+

Zearalenone

+

++

+++

Fumonisin

+

+

+++

Deoxynivalenol

+

++

++

Slight toxicity (+) Moderate toxicity (++) High toxicity (+++)

Aflatoxins Amongst several types of mycotoxins, aflatoxins are highly toxic, carcinogenic, and cause severe contamination (Manafi, 2012).

Approximately 15 types of aflatoxins have

AFB1

been described, among which “B” and “G” families (aflatoxins B1, B2, G1, and G2) are particularly dangerous to livestock as they have been found in all major food crops, grains, and their derived products utilized for animal and poultry nutrition.

Among all aflatoxins, aflatoxin B1 (AFB1) is a key toxin that is tightly regulated and monitored in very small quantities (at minor pbb levels) in agricultural commodities to be used by the animal

(EUR-Lex., 2021).

These naturally occurring compounds are produced mainly by the fungi Aspergillus

flavus, Aspergillus parasiticus and Aspergillus nomius, leading to serious health consequences through contamination of a wide variety of food such as maize which can be used in animal/poultry feeding (Manafi et al., 2018b).

Aflatoxicosis, a disease that occurs when large doses of aflatoxins lead to acute poisoning, is life-threatening, usually due to liver damage to the liver. Other adverse effects of aflatoxins are:

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Poor performance Immunosuppression and Increased susceptibility to infections Increased the susceptibility to other diseases and mortality Drop in egg production and egg weight Decreased hatchability and hatchling weight Increased liver fat and decreased activity of several liver enzymes Changes in organ weights Reduction in serum protein levels Carcass bruising and poor pigmentation

Although the concentration level, dietary exposure period, sex, species, age,

(Manafi et al., 2009; Manafi et al., 2012b; Manafi et al., 2012c; Manafi et al., 2012d; Manafi et al., 2012e; Manafi et al., 2014a; Hedayati et al., 2014a; Manafi et al., 2014b; Hedayati et al., 2014b; Manafi et al., 2014c; Manafi et al., 2015a; Manafi et al., 2016; Manafi et al., 2018b; Manafi et al., 2018c; Manafi, 2018; Eskola et al., 2020).

breed, and health status of animals are different factors that affect the level of toxicity in poultry, all avian species, especially younger ones (chicks, goslings, ducklings, and turkey poults) are the most susceptible to AFB1 toxicity (Manafi

et al., 2012b; Manafi et al., 2014d).

FACTORS

AFB1

EFFECTS Poor performance

Concentration Dietary exposure period Sex Species Age Breed Health status

Egg production Egg weight Hatchability Hatching weight Liver fat Liver enzyme activity Changes in organ weights Protein serum levels

Figure 1. Factors and effects associated with aflatoxin exposure in poultry.

Carcass bruising Poor pigmentation Immunosuppresion Susceptibility to infections and other diseases Mortality

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Ochratoxins Ochratoxins are a group of naturally occurring

Increased relative weight of the liver, kidney,

foodborne mycotoxins found in a wide variety

spleen, pancreas, proventriculus, gizzard, heart

of agricultural goods worldwide, including staple food crops, cereal grains, dried fruits, and nuts.

They are produced by some Aspergillus species (mainly A. ochraceus, A.

carbonarius and A. niger) and some Penicillium species, especially P. verrucosum (Manafi et al., 2011).

Ochratoxins represent three secondary metabolite forms (A, B, and C), among which Ochratoxin A is the most prevalent fungal toxin of its family.

Ochratoxin A is known to have nephrotoxic, teratogenic, immunosuppressive, and hepatotoxic effects in many animal species.

Increased mortality Poor feed conversion efficiency Reduced relative weight of the Bursa of Fabricius

(Giambrone et al., 1985; Gibson et al., 1989; Scudamore, 2005; Martins et al., 2008; Manafi et al., 2009; Manafi et al., 2011) The effects of ochratoxin A in poultry have been found to be quite pronounced in younger animals (Dortant et al., 2001).

In birds, the kidney is the main and primary organ affected, and a marked decrease in the percentage of circulating lymphocytes and

Its possible carcinogenic effects on

a significant increase in the percentage of

humans, which could be caused through

inflammatory cells (monocytes and heterophils)

the consumption of toxins accumulated

are also reported (Moura et al., 2004).

in animal meat, are also reported by scientists (Scudamore, 1996).

Ochratoxin A also inhibits protein synthesis and lipid peroxidation. The latter could be related to oxidative damage which impairs the overall quality and safety of animals (Stander et al., 2000).

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Poor performance Poor FCR

Consumption of ochratoxin A by poultry may lead to:

Age-specific pathological lesions Kidney, liver, spleen, pancreas, proventriculus, gizzard and heart weight

Decrease in farm productive performance Subcutaneous hemorrhage Immunosuppression Increased age-specific pathological lesions

Immunosuppression Mortality Weight of the Bursa of Fabricius % Circulating lymphocytes Inflammatory cells

Figure 2. Ochratoxinassociated effects in poultry.

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T-2 toxin Reported in many parts of the world, trichothecenes are produced as secondary metabolites (T-2 toxin being the earliest investigated and amongst the most toxic members of this family), mainly by fungi of the genus Fusarium of which the most important species are F. sporotrichioides, F.

langsethiae, F. acuminatum and F. poae, commonly found in various cereal crops (wheat, corn, barley, oats, rye, etc.) but also in soy meal (Manafi et al., 2015b).

Routinely, the high-pressure liquid chromatography (HPLC) is used to detect the T-2 toxin in a wide range of feed and food.

Lipid peroxidation High fever

These compounds are generally very stable and are not degraded during

Muscle and skin necrosis, as well as

storage/milling and high temperatures

bacterial infections of the necrotic tissues

of cooking/processing of food.

The toxicity and deleterious effects of T-2

Enlarged lymph nodes Delayed ovulation

toxin vary based on numerous factors, such as the administration route, the

Decreased sperm motility and increased

exposure time, the administered dosage,

sperm morphological abnormalities

and the age, sex, and overall health of the animal (Hossam et al., 2013).

Inhibition of protein, DNA, and RNA synthesis Cytotoxicity

T-2 toxin is believed to increase oxygen radical production hence resulting in direct cell injury (Manafi et al., 2012f).

Immunomodulation Lesions in the digestive tract, organs, and skin

Studies indicating that T-2-toxin ingestion in high doses by poultry through the contaminated grain, hay, and straw causes:

Neural disturbances and nervous disorders Declined performance and production parameters due to feeding refusal

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Bloody diarrhea, bone marrow, buccal lesions, serous hemorrhagic inflammation Dystrophy in liver, kidney, heart, brain and peripheral ganglia of the vegetative nervous system

(Kalantari et al., 1989; Zian et al., 2011; Kachuei et al., 2014; Krska et al., 2014; Drakulic et al., 2016; Yuan et al., 2016).

Preliminary, the liver is one of the first target organs where the enzymes help metabolize drugs that pass through the liver. The decrease in its activity could lead to an increase of unmetabolized drugs in the plasma, which can pose a dangerous risk for the animal’s health (Goossens et al., 2013).

FACTORS Administration route Exposure time Dosage Age Sex Health status

The increased elevation of glutathione disulfide and 3-hydroxybutyrate suggests that the T-2 toxin promotes an anti-oxidative response in organ systems and helps with free radical generation.

T-2 toxin EFFECTS Muscle and skin necrosis Lesions in digestive tract and other organs

Delayed ovulation Sperm motility Sperm abnormalities Neural disturbances Nervous disorders

Inhibition of protein, DNA and RNA synthesis Dystrophy in liver, kidney, heart, brain and peripheral ganglia of the vegetative nervous system

Immunomodulation Cytotoxicity Bloody diahrrea Serous hemorrhagic inflammation Declined performance and production parameters due to feeding refusal

Figure 3. Factors and effects associated to T-2 toxin exposure in poultry.

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CONCLUSIONS Concentrations of mycotoxins in the feed are usually low and their immunosuppressive effects and secondary infections often make diagnosis difficult.

If at the onset of the disease, a change in the diet leads to health and performance improvements in animals, this may point to mycotoxin poisoning.

Although it is difficult to predict the effect of multiple toxins, certain studies confirm that presence of combined mycotoxins in animal feed show more severe impacts on livestock health and productivity.

Monitoring and controlling all the feed ingredients which are to be used for poultry diets is not always practical and could seriously compromise the world food supply, due to very large quantities, however, regular control of grain and feed samples is a valuable preventive measure and it is only accurate if representative samples are tested in the laboratory.

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