OBJECTIVE
In this report a multi-mycotoxin method, based on solid-liquid extraction (SLE} for analysis of 36 mycotoxins using Agilent 6460c UHPLC-MS/MS is described.
The 36 mycotoxins included were:
European Union (EU) regulated and related mycotoxins
Aflatoxins (AFB1, AFB2, AFG1, AFG2)
Deoxynivalenol (DON)
Fumonisins (FB1, FB2, FB3, FB4)
HT-2 toxin (HT-2)
Ochratoxin A (OTA)
T-2 toxin (T-2)
Zearalenone (ZEN)
Emerging mycotoxins
Beauvericin (BEA)
Enniatins (ENNA, ENNA1, ENNB, ENNB1)
Fusaric acid (FA)
Moniliformin (MON)
Other mycotoxins
15-acetyl deoxynivalenol (15-ADON)
3-acetyl deoxynivalenol (3-ADON)
Deoxynivalenol-3-glucoside (D3G)
Citrinin (CIT)
Alternariol (AOH)
Diacetoxyscirpenol (DAS)
Ergosine (ES)
Ergocryptine (ECR)
Neosolaniol (NEO)
Nivalenol (NIV)
Patulin (PAT)
Tenuazonic acid (TeA)
Fusarenon-X (FX)
Zearalanone (ZAN)
α-zearalenol (α-ZEL)
β-zearalenol (β-ZEL)
HPLC column Analytical column Waters CORTECS UPLC® 18 2.1 x 100 mm, 1.6 μm
HPLC Guard Column Guard column Waters CORTECS® UPLC C18 VanGuard™ 2.1 x 5 mm, 1.6 μm
Mobile phase A: 0.1 % formic acid, 5 mM ammonium formate in water B: 0.1 % formic acid, 5 mM ammonium formate in methanol/acetonitrile
Flow rate 0.300 ml/min
lnjection volume 5 μL
RESULTS & CONCLUSIONS
The recoveries of 36 mycotoxins in spiked food, feed, and cereal matrices ranged from 67.4 % to 121.1 % after internal standard correction, with relative standard deviations (RSDs) below 14.9 %.
These results demonstrate that the dilute and shoot method provides a robust approach for the simultaneous analysis of 36 mycotoxins using LC-MS/MS.