Appendix 79 POSTER SESSION Chairperson: Dr Nilay UNAL Rapporteur : Dr Stéphan ZIENTARA Dr Georgi GEORGIEV At the Open Session of the EUFMD Research Group Meeting 17 posters were presented. These posters deal with -Epidemiology (1) -FMDV structure (1) -FMD vaccines (2) -Pathogenesis (4) -Diagnosis (molecular diagnosis-7) (serological diagnosis-2) Recommendations of the Poster Session The reporting group would like to underline the fact that the decision of the FMD Research Group to organise such a poster session was a good initiative. On the basis of the presented posters in this session the reporting group makes following recommendations: -
necessıty to reinforce the surveillance of FMD around the world in particular in Africa develop studies on the structure of the FMDV proteins in order to better understand their fonctions and develop antiviral molecules develop reagents (recombinant antıbodies or monoclonal antibodies) for diagnosıs or studies on pathogenesis develop and reinforce studies on the pathogenesis of FMD and the interactions virus\cells develop studies on the immune response against FMDV in susceptible species develop studies on the molecular diagnosis of FMDV (real-time PCR, loop mediated amplification, rolling circle amplification) develop tools for rapid and reliable diagnostic method for serological diagnosis of FMD necessity of comparative studies, validatıon and standardisatıon new diagnostic methods
Summary of the posters presented Identification of FMDV replication in cells within the foot and tongue epithelia S. Durand1*, C. Murphy1, S. Alexandersen1,2. Pirbright Laboratory, Institute for Animal Health, Ash Rd, Woking, Surrey, GU24 0NF, UK. 2 Present address: Danish Institute for Food and Veterinary Research, Department of Virology, Lindholm, DK4771 Kalvehave, Denmark. 1
This study deals with identification of FMDV replication sites by in situ hybridisation in pigs. In situ hybridization (ISH) has been used to detect FMDV RNA in tissues from infected pigs. A digoxigeninlabelled RNA probe corresponding to a coding part of the RNA-dependent RNA polymerase (3D) genomic region was prepared. Results indicate that the basal cell appears to be the cell type demonstrating the highest signal for the detection of the FMDV positive sense RNA in both tongue and foot epithelium. The detection FMDV positive sense RNA showed very strong signal in basal cells (especially in foot lesions). Mouth lesions showed in general less signals than in foot lesions.Although the stratum spinosum cells show more signs of cytolysis than the basal cells, the FMDV RNA signal in the stratum spinosum cells was more diffuse and less concentrated. These results are strongly suggesting that the basal cells could be the early replication site of FMDV in vivo. Laser Micro-Dissection studies of FMDV infection in pigs R. Ahmed1*, S. Durand1, Z. Zhang1, M. Quan1, C. Murphy1 and S. Alexandersen2 Institute for Animal Health, Pirbright, Woking, Surrey, GU24 ONF, U.K. 2 Danish Institute for Food and Veterinary Research, Department of Virology, Lindholm, DK-4771 Kalvehave, Denmark 1
The objective of this study was to isolate and quantify foot-and-mouth disease virus (FMDV) in the different epithelium cell-types in order to observe potential differences in FMDV RNA distribution in specific tissues. Laser Micro-Dissection (LMD) was carried out on frozen sections from selected tissues of infected pigs. After RNA extraction the samples were tested for FMDV and 18S ribosomal RNA (as a RNA marker) by real time quantitative RT-PCR.
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