Appendix 73

Appendix 73

A serosurvey to measure antibody levels to FMDV and trace antibodies to FMDV NSPs following Spring 2004 FMD vaccination campaign in Turkish Thrace Region

A.N. Bulut*, B. Sareyyupoglu, U. Parlak and C. Cokcalıskan FMD (Şap) Institute P. Box:714 Eskisehir Rd, 06044, Ankara ,TURKEY

Introduction

To evaluate protection levels and particularly to investigate infected animal in Turkish Thrace by carrying out FMD NSP ELISA, a serosurvey was carried out. In this serosurveillance a total of 4792 sera were collected and tested by LPB ELISA to measure antibody levels and by FMD NSP ELISA to differentiate infected animals from vaccinated animals. After the serosurvey, field epidemiological investigations were conducted at locations where NSP antibodies were detected in a number of randomly-sampled animals (i.e. where there was “clustering” of NSP seropositive animals), so as to assess the status of NSP antibody positive animals. This paper provides an epidemiological assessment of serosurveillance and follow up survaillance in field condition.

Materials and Methods:

1. LPBE was carried out to measure antibody to FMDV. 2. An FMD NSP ELISA (ready to use kit) was used to detect previously-infected animals. 3. A follow up field investigation was conducted in the case of NSP seropositive animals. 4. Virus isolation was attempted from probang (OP fluid) samples collected during follow up investigations.

Results

As this work is still ongoing, all results will not be available until presented at the meeting.