An
InnovativeInjectable,Retrievable,andSustainableDrugDeliverySystemforEndodontic
AadiBordia,CandyDeng,EliKrasnoff,LindaLiang,BrennaRen,MiraSetia,ZoeKatevatis,AbdelH.Mahmoud,AaronSloutski,MiriamRafailovich
-RAMAN,XRD,andFT-IRanalysisofCASApasteandhydrogels wereconducted.
-Presenceofco-crystalsincludingcalciumsalicylateandothersaltsare formedthroughhydrogenbonding
- Co-crystals likelyimprovesCASA'sdrugperformancethrough increasedsolubilityandstability
-FDMA/CASAresemblesPEOcomposition
-CASA1:6demonstrateshighestdegreeofsimilarityto CalciumSalicylate
AnInnovativeInjectable,
Retrievable,and
SustainableDrugDeliverySystemforEndodonticTherapy
AadiBordia,CandyDeng,EliKrasnoff,LindaLiang,BrennaRen,MiraSetia,ZoeKatevatis,AbdelH.Mahmoud,AaronSloutski,MiriamRafailovich
HydrogelPerformance
Efficacy
Crosslinking Efficiency Biopolymer titration
• CASAreduces bufferproperties ofbiopolymer
• IndicatesCASA's abilitytodestroy microbialcell wall
Rheology
Conclusions
1. OptimalratioofCASAis1:6.
2. EncapsulationofCASAin F127-DMAdecreasedits viscosity.
3. F127-DMA/CASAhydrogelis injectable&retrievable insideaclearrootcanal model.
4. F127-DMA/CASAhasanear neutralpHoverD3&80.9% crosslinkingefficiency.
5. CASAincreasedthe hydrophilicityofhydrogel.
6. Discoveryofnewchemical compounds(salicylatesalts) inCASApaste→further chemicalcomposition validationmaybeneeded
7. F127-LA/CASA’sapplications (degradable)couldbe explored.
• E.faecalis and S.mutans have highlysimilarRAMANspectra
• Bandshapechangeat 1208cm-1(Tryptophanand phenylalanine)and1300 cm-1(C-Hdeformations),which isattributedtofibrinogen
• Shoulderpeakat987cm-1 indicatesfibrinogenmaybe partiallyabsorbed.
● TheSEMimageofthe Bovinetoothslice showsleftovervital pulptissuethatshows thenecessityof sterilizationusing sodiumhypochlorite EDTAandautoclaving
SelectedReferences
[1]Alghamdi,Faisal,andMarwaShakir.“TheInfluenceof EnterococcusFaecalisasaDentalRootCanalPathogenon EndodonticTreatment:ASystematicReview.”Cureus,U.S. NationalLibraryofMedicine,13Mar.2020, www.ncbi.nlm.nih.gov/pmc/articles/PMC7152576/ [2]Matsumoto-Nakano,Michiyo.“RoleofStreptococcus MutansSurfaceProteinsforBiofilmFormation.”TheJapanese DentalScienceReview,U.S.NationalLibraryofMedicine, Feb.2018,www.ncbi.nlm.nih.gov/pmc/articles/PMC5884221/ [3]Sillanpää,Jouko,etal.“AFamilyofFibrinogen-Binding MscrammsfromEnterococcusFaecalis.”HoustonMethodist Scholars,SocietyforGeneralMicrobiology,19June2015, scholars.houstonmethodist.org/en/publications/a-family-of-fibr inogen-binding-mscramms-from-enterococcus-faecali