Sucrose Density Gradient Centrifugation

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2 Sucrose Density Gradient Centrifugation

nents

Centrifugation Types

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INTRODUCTION OF COMMON CENTRIFUGATION TYPES

 Differential centrifugation  Rate-zonal centrifugation  Isopycnic centrifugation

01

Centrifugation Types INTRODUCTION OF COMMON CENTRIFUGATION TYPES

Differential Differential Centrifugation Centrifugation A simple form of centrifugation.

•

Performed at different centrifugal speeds.

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•

Density Density Gradient Gradient Centrifugation Centrifugation Density gradient centrifugation is divided into two types: •

Rate-zonal centrifugation

•

Isopycnic centrifugation

Centrifugation Types INTRODUCTION OF COMMON CENTRIFUGATION TYPES

01

Differential Centrifugation

• Gradually increasing the centrifugal speed to separate

02

Rate-zonal Centrifugation

• Relies on the different

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sedimentation coefficients

particles with different

• Density of the gradients is

sedimentation speeds

lower than the density of

• Separation of particles with large differences in sedimentation coefficient

samples, and the bands are formed during the sedimentation process

03

Isopycnic Centrifugation

• Particles are separated based on their density • The particles settle completely to the position of the gradient medium with the same density as the sample

02

Sucrose Density Gradient Centrifugation

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 Gradient Preparation  Centrifugation  Separation and Elution

Sucrose Density Gradient Centrifugation PROTOCOL FOR SUCROSE DENSITY GRADIENT CENTRIFUGATION

Gradient Preparation 02

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01 Sucrose solution (66%, w/w)

NOTE:

Sucrose solutions with different concentrations

Filtration

• Position of the pipette tips

03

Sucrose gradient preparation

• Avoid disturbing the interface Concentration Verification between layers • Use as soon as possible

Gradient Forming Instrument

Sucrose Density Gradient Centrifugation PROTOCOL FOR SUCROSE DENSITY GRADIENT CENTRIFUGATION

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01 Add sample

Balance

Pre-Experiment （ Rotor & Centrifuge ）

03

• Centrifugal Time • Centrifugal Force

Centrifugation

Bands generation

Sucrose Density Gradient Centrifugation PROTOCOL FOR SUCROSE DENSITY GRADIENT CENTRIFUGATION

Separation and Elution 02 感谢您下载包图网平台上提供的 PPT 作品，为了您和包图网以及原创作者的利益，请勿复制、传播、销售，否则将承担法律责任！包图网将对作品进行维权，按照传播下载次数进行十倍的索取赔偿！

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Separati on

01 Elution

03

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