CreativeBioMartEnhancedItsBacterialExpressionServiceforResearchUse
CreativeBioMart,abiotechnologycompanythatsuppliesreagentsforthescience community,recentlyenhanceditsbacterialexpressionservices,aproofofitsgoaltoprovide advancedtoolsforproteinexpressionandpurificationresearch
Escherichiacoli(Ecoli)wasdiscoveredbyEscherichin1885Escherichiacoliisan opportunisticpathogenicbacteriumthatcancauseinfectionsinvarioustissuesandorgans suchasthegastrointestinaltract/urethraofhumansandvariousanimalsundercertain conditionsEscherichiacolibelongstothecoliform-typeanaerobicGram-negativebacteria thatusuallyexistintheintestinaltractoforganismsMostEcolistrainsareharmless,but certainserotypescancauseseverefoodpoisoninginthehost
TheharmlessEcoliispartofthenormalgutfloraandlivesinsymbiosiswiththisorganism, whichcanbenefitthebodybyproducingvitaminK2andpreventthespreadofpathogensin thegutEscherichiacoliisausefulhoststrainforrecombinantproteinproductionIthasthe advantagesofcleargeneticbackground,simplecultureoperation,hightransformationand transductionefficiency,fastgrowthandreproduction,lowcost,andrapidmassproduction oftargetproteinsMoreover,theexpressionlevelofexogenousgeneproductsismuch higherthanothergeneexpressionsystems,andtheamountofexpressedproteincan exceed30%ofthetotalbacterialproteinTherefore,theEcoliexpressionsystemisawidely usedproteinexpressionsystem,andabout30%ofcommerciallyavailablerecombinant proteinproductsareproducedbytheEcolisystem
TheEcoliexpressionplatformfromCreativeBioMartgathersavarietyofbacterial expressionvectorswithvariousN-andC-terminaltagsandhoststrainstoguaranteethe selectionofthemosteffectivesystemRegardinginsolubleproteins,skilledPhD-level scientistsatCreativeBioMarthavecreatedasuccessfulproteinrefoldingsystemthatconsists ofanimprovedrefoldingbufferandprocedureRegardingfermentationscale,theteamhas bioreactorsthatrangeinsizefrom5to20,000literstomeetdiversifiedneeds
TheBacillussubtilissystemfromCreativeBioMartisagreatchoiceforthehigh-level expressionofmonomericproteinproductsWhencombinedwithitssecretfermentation protocols,thissystemenablesrapidprocessdevelopmentandscale-uptosupportearly clinicaltesting
OtherproteinexpressionservicesarealsoavailableatCreativeBioMart,covering:
Baculovirus-InsectCellexpressionSystems(SF9,SF21,S2,Tni/HI-5),Cell-FreeinVitro Proteinexpression,MammalianexpressionSystems(CHO/HEK293),Yeastexpression Systems(Ppastoris/Scerevisiae),High-throughputProteinProduction,etc
Toknowmoredetailedinformationaboutthebacterialproteinexpressionserviceprovided by Creative BioMart, please visit https://wwwcreativebiomartnet/bacterial-expression-systemshtm