ANALYSIS OF MICROBIOME (PLANKTON ) OF FRESHWATER , ESTURINE WATER AND MARINE WATER OF DAKSHINA KANNADA DISTRICT , KARNATAKA. Abhisheka G, Purushothama K, Punith Kumar G and Chandra M* Department of Biosciences, Mangalore University, Mangalagangothri-574 199, Dakshina Kannada District, Karnataka, India
INTRODUCTION
In the aquatic ecosystem, the microbiome includes Macroalgae, microalgae, and bacteria, and the interaction of biotic and abiotic factors is seen in the aquatic ecosystem, it affects the distribution of microbiome in the aquatic ecosystem. The distribution of microbiome varies in freshwater, estuarine, and marine water ecosystems. Planktons are the autotrophic community of aquatic ecosystem and major source of food to other living components of the aquatic ecosystem. Presence of these planktons in the water bodies are affected by physico and chemical parameters of the water body. About 1% of the global biomass is due to phytoplankton. MATERIALS AND METHODS
The plankton samples are collected from Narahari parvatha (Freshwater), Nethravathi estuary (Estuarine water), Someshwara beach (Marine water). The plankton samples are collected using Forceps in sampling bottles and preserved using 5%. The preserved samples were brought to the laboratory. Then the slides were prepared in the laboratory and then the planktons are identified using a microscope with the help project guide. Some of the Physico-chemical properties of water samples such as temperature, ph, salinity, dissolved oxygen, alkalinity were also measured using titrimetric methods in the laboratory to understand the reason for variation in the distribution of plankton microbiome of these three aquatic ecosystems.
RESULTS AND DISCUSSION DISTRIBUTION OF PHYTOPLANKTONES IN THE COLLECTED WATER SAMPLES
PHYTOPLANKTON
NARAHARI PARVATHA (POND ECOSYSTEM)
NETHRAVATHI ESTUARY(ESTURAINE ECOSYSTEM)
Oscillatoria homogenea
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Cymbella yarrensis
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Lyngbya gracilis
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Lyngbya sp.
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Pinnularia alpina
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Diploneis aestiva
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Diploneis sp.
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Spirogyra pratensis
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Spirogyra sp.
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Ulothrix zonata
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Ulothrix aequalis
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Aphanocapsa littoralis
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Merismopedia sp.
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Ulva reticulata
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Gracillaria sp.
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Chetomorpha sp.
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In the present study, various kinds of planktons were identified in all the collected samples. The distribution of all the plankton species is also compared in all the three water bodies. Some of the identified plankton species include Oscillatoria homogenia, Cymbella yarrensis, Lyngbya gracillis, Pinnularia alpina, Diploneis aestiva, Spirogyra pratensis, Ulothrix zonata, Ulva reticulata, etc. Some of the plankton species present in the freshwater samples were absent in the marine water sample due to the high salinity of the marine ecosystem. Due to the constant mixing of freshwater and marine water in the estuarine ecosystem, some of the common freshwater plankton species were in seen estuarine ecosystem.
ACKNOWLEDGEMENT CONCLUSION In the study different plankton species were identified, some of the species were unknown under genus like Lyngbya, Diploneis, Gracillaraia, etc. In the study, it is understood that the distribution of plankton species in different ecosystems depends on the Physicochemical properties of the water bodies.
SOMESHWARA SEA(MARINE ECOSYSTEM)
The authors are thankful to Dr. Sharath Chandra K and Laveen K.B, Faculty, Department of Biosciences, Mangalore University for their continuous guidance in identifying the plankton species, and to the first M.Sc Biosciences students and Department of Biosciences, Mangalore University for helping to collect the samples and for providing laboratory and equipment to carry the study.
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