Stem cell poster 1

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Poster Presentation : ‘Online Digital Poster Presentation_Biotecnika’ Phycoremediation by using Algae

Presented by ; Debasmita Chakraborty from UEM, Kolkata in ‘Online Digital Poster Presentation’ event at Biotecnika

Abstract

Introduction

Discharge of untreated domestic and industrial wastewater into aquatic bodies is posing a serious eutrophication threat, leading to a slow degradation of the water resources. A number of physical, chemical and biological methods have been developed for the treatment of wastewaters; among these, the use of microalgae is considered as a more eco-friendly and economical approaches. Microalgae are versatile organisms which perform multiple roles in the environment—bioremediation of wastewater, gleaning of excess nutrients and in turn, generate valuable biomass which finds applications in the food, biofuel and pharmaceutical industries. They are currently being utilized to reduce the high nutrient load (especially N and P) from wastewaters.

Results & Observation

Seawater(sali ne) = 97.50%

Polar Ice & Glaciers = 2.50%

Available fresh water = 0.01%

• -Identified by morphological characteristics • -Identified as Chlorella sp. • Strain Name Form

Issues related to environmental pollution are becoming more serious with the increasing population, urbanization, industrialization and their indirect effects on ecosystem services (Rawat et al. 2011; Sood et al. 2012). Even mixing of untreated wastewater in aquatic bodies is emerging up as one of the major issues that is challenging the stability of nations (Renuka et al. 2014; Yang et al. 2008).

Size Shape Pigmentation

• Chlorella sp. Moderate Almost Regular

Greenish

Elevation Elevated

Round

Phycoremediation effectiveness: BOD & COD removal with days

• Phycoremediation is a process of using algae to bio-remediate wastes or wastewaters. The biodiversity of microalgae is enormous and estimated to be about 200,000–800,000 species, out of which about 50,000 species are only described (Starckx 2012).

Materials & Methods

Advantages of Phycoremediation:• 1.Reduces Pollutant load

• Isolation of the Algal Species: Isolated from pond water inside the Jadavpur University campus. • Culture Media Preparation: Bold’s Basal medium(BBM) was used composed of NaNO325 g/l, MgSO4,7 H2O 7.5 g/l, NaCl2.5 g/l, K2HPO47.5 g/l, KH2PO417.5 g/l and CaCl2, 2 H2O 2.5 g/l.

Total Kjehldal Nitrogen & Phosphorus removal with days:

• 2.Eco-friendly process • 3.Produces Biomass • 4.Cost effective treatment Etc… Headin Headin Headin g g g

• Inoculation: 1 ml of diluted sample wastewater (1:100) was added to the 50 ml of culture medium(BBM) • Kept for 10 days at 20oC under illumination

Item Item Item

122 • T759 228

233 856 134

345 290 238

Item Item

954 324

875 325

976 301

• Synthetic Dairy Wastewater Preparation: prepared using dry milk powder of the brand “Amul” .Synthetic Dairy waste water was composed of carbohydrate, protein, fat, Na, K, Ca, Cl, P and other trace metals.

Item

199

137

186

Objectives & Applications

• Identification of the Most Potent Strain

Observation

Conclusions

• The available literature in reports have clearly emphasized and proved beyond doubt that microalgae are efficient in nutrient removal from different types of wastewaters and can be explored for the remediation of these contaminated sites. The response and growth of different types of microalgae in wastewater also vary, because of differences in their inherent ability, especially nutrient uptake; tolerance to harsh/extreme environmental conditions and competitive potential, vis-a-vis indigenous organisms. • Chlorella sp. is very much efficient in removing nutrients(mainly Phosphorus) from wastewater. • BOD and COD reduction percentage is slightly less when compared with phosphorus removal data.

Reuse of waste water after Phycoremediation

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8/21/2020

Online Digital Poster Presentation _ Biotecnika

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