ACUTE TOXICITY OF AN AZOLE FUNGICIDE TEBUCONAZOLE ON IONIC REGULATION AND NA+/K+-ATPASE ACTIVITY IN A FRESHWATER FISH CIRRHINUS MRIGALA S. Subbiah, M. Ramesh*
Toxicology Laboratory, Department of Zoology, Bharathiar University, Coimbatore- 641046, Tamil Nadu *E-mail: mathanramesh@yahoo.com
Control
Control
Treatment
Experiment
140 ** +11.95
120
100
80
60
40
20
0
Plasma Na+/K+ -ATPase (Âľg/h/g)
45
Plasma chloride (mmol/L)
ABSTRACT-Published original work Tebuconazole (TCZ), an azole fungicide has been ordinarily utilized in an assortment of industry and farming items. TCZ is persevering in the earth and in this way representing a hazard to sea-going life forms. The current examination is expected to explore the intense harmfulness of TCZ on ionic regulation and Na+/K+-ATPase action in a freshwater fish Cirrhinus mrigala. The middle deadly centralization of TCZ for 24 and 96 h (with 95% certainty limits) was seen as 9.6 and 7.2 mg L-1, individually. In intense treatment, plasma sodium and potassium levels were seen as diminished fundamentally (p<0.01) in TCZ treated fish while a huge increment in plasma chloride level and gill Na+/K+-ATPase movement was watched.
** +138.15
40 35 30 25 20 15 10 5 0 Exposure period (96h)
Exposure period (96h)
BACKGROUND The point of this examination was to assess the cell harm in the fish Cirrhinus mrigala presented to Tebuconazole utilizing Gill Na+/K+ ATPase movement and the plasma electrolyte levels-related biomarkers.
RATIONALE
fungicide are risky to amphibian biological systems, as it impacts the biochemical profile of fish and this reality ought to be thought about when this compound is utilized in different business items.
CONCLUSION
It is reasoned that fungicide are perilous to oceanic biological systems, as it impacts the biochemical profile of fish and this reality ought to be thought about when this compound is utilized in different business products. More ever the taken up boundaries could be adequately utilized as potential biomarker of fungicide toxicity(TCZ) to the freshwater fish in the field of natural biomonitoring. REFERENCES Campbell, R.C.1981.statistics for Biologists,2nd Ed., Cambridge University Press, Cambridge London,pp.66.
STRATEGY 1.Procurement and acclimatization of fish
Shiosaka, T., Okuda, H., Fungi, S., 1971. Mechanisms of phophorylation of thymidine by the culture filtrate of Clostridium perfringens and rat liver
2.Fish exposure and sampling Gill Na+/K+ ATPase activity (Shiosaka et al. (1971) Sodium and Potassium level(Sunderman et al. (1959) Chloride level(Schales and Schales (1941) Studentsâ&#x20AC;&#x2122;t-test (Campbell,1981).
extract. Biochem. Biophys. Acta. 246, 171-183. Sunderman, F.W. Jr., and Sunderman, F.W., 1959. The rapid colorimetric estimation of potassium. Am. J. Clin. Path. 29: 95. Schales, O., Schales, S., 1941. A simple and accurate method for the determination of Chloride in biologival fluids. J. Biol. Chem. 140: 879 - 884.
RESULTS Changes in the plasma electrolytes and gill Na+/K+ATPase activity of a fresh water fish Cirrhinus mrigala treated with acute(96h) concentration of Tebuconazole. Control
Control
Treatment
Treatment
12
10 200 ** -15.51
150
100
50
Plasma potassium (mmol/L)
Plasma sodium (mmol/L)
250
8 ** -33.13 6
4
2
0 0 Exposure period (96h) Exposure period (96h)