Issuu on Google+

The future in animal feed Feeding the world an increasingly scarce, though essential nutrient


1


Table of contents

About Unibio

4

The concept

5

The U-Loop® technology

6

UniProtein®

7

UniProtein® in the protein demand chain

8

Environmentally friendly production

9

Chemical composition

10

Seven reasons to use UniProtein® in animal diets

11

UniProtein® in pig diets

13

UniProtein® in broiler diets

15

UniProtein® in mink diets

20

UniProtein® in blue fox diets

22

UniProtein® in aqua diets

23

The proper use of UniProtein® in animal diets

26

2


4

Met han eC H

ÂŽ fermento r plan p o o t U-L

ÂŽ

n otei iPr Un

lef

An

at

im

me

al f

re &

eed

Manu

to

ve

rs

A n i m a l p ro d u c t s

Future continuity cycle

3


About Unibio Unibio is a Danish biotechnology company with a core focus on fermentation technologies, allowing a highly scalable production of bacterial protein meal. Since its foundation the company’s goal has been to supply the world with innovative sustainable solutions to overcome the food challenge faced by the growing global population. With the use of Unibio’s biotechnological innovations the company is able to transform processes that occur in nature every day into a bioindustrial setting and produce healthy sustainable protein for animals in an eco-friendly way.

Unibio owns the intellectual property rights to a unique fermentation technology – the U-Loop® technology – producing a high-value bacterial protein product, UniProtein®. This novel protein source disrupts the traditional food value chain while mitigating the traditional challenges by decoupling protein production from the fluctuating agriculture and stressed fishing industries. Crop land can thus be used to produce food for humans instead of feed for animals. UniProtein® is a sustainable solution to the world’s protein-scarcity challenge.

4


The concept

Carbon dioxide and unused nitrogen from the air are let out

Fed into the fermentor : -Air -Methane -Minerals -Oxygen -Catalysts

Drying tower U-LoopÂŽ fermentor

Viscous protein broth is sprayed out

This process occurs in nature every day e.g. in lakes

Process: Bacteria eat methane, and the liquid is transformed to protein broth

Propeller pump keeps the liquid moving Hot air from the bottom of the fermentor dries the broth, turns it into protein granule and makes it fall to the bottom

Temperature is held at 45�C

Cooling water

Separator

Separator separates water from the protein broth

5

Protein granules are excellent animal feed


The U-Loop® technology 33

Normalized nutrient transfer rate

The U-Loop® technology provides optimal growth conditions for the bacteria. The tailor-made technology speeds up the natural process significantly while allowing the execution of the procedure in a controlled and monitored environment. Unibio has developed a vertical fermentor design that can handle a large volumetric gas fraction, significantly improving the average productivity. The technology has been developed in cooperation with the Technical University of Denmark (DTU) after more than 30 years of R&D and is patented under process patent No PA199900690.

2,5 2.5

22 1,5 1.5 11 0,5 0.5

00 0.2 0.2

0.4 0.4

0.6

0.8

0.6

0.8

1

1

Normalized energy consumption U-Loop

Conventional Bioreactor

Conventional bioreactor

®

U-Loop®

The U-Loop® fermentor allows for a much more energy-efficient transport of nutrients from gas phase into liquid phase, at a much higher rate compared to conventional bioreactors.

6


UniProtein® UniProtein® is a brand-specific bacterial protein meal. It is a protein-rich biomass produced by a microbial culture with methane as the sole carbon and energy source. UniProtein® contains the following four heat-killed bacteria: Methylococcus capsulatus (90%) (NCIMB strain 11132) Ralstonia sp. (8%) (NCIMB strain 13287, formerly known as Alcaligenes acidovorans) Aneurinibacillus danicus (1%) (NCIMB strain 13288, formerly known as Bacillus brevis) Bacillus firmus (1%) (NCIMB strain 13288)

Methane

The production process always results in a uniform product.

Oxygen Ammonia Minerals M. capsulatus

The substrates for making UniProtein® are: a nitrogen source (ammonia), an energy source (methane or methanol), Single cell protein minerals and oxygen. (UniProtein®)

UniProtein® was approved for animal nutrition in the EU in July 1995 according to Commission Directive 95/33/EC. Under EU Commission Regulation No 68/2013 of 16 January 2013, UniProtein® is listed on the catalogue of feed materials (under 12.1.2: Protein from Methylococcus capsulatus (Bath), Alcaligenes acidovorans, Bacillus brevis and Bacillus firmus).

UniProtein® is well suited as a protein feed material for livestock is a free-flowing reddish-brown granule with a particle size of 150-200μm has a consistent chemical composition and protein content has a long shelf life does not contain genetically modified microorganisms (non-GMO) is a non-polluting product as it is produced by a microbial culture with methane is free from toxins, dioxin and heavy metals due to the controlled production process and the fact that all minerals used are food grade addresses the world’s growing need for animal protein, which will continue to increase in line with population growth is produced without using any pesticides or fertilizers is weather-independent production does not require any arable land and utilizes a minimum amount of water

7


UniProtein® in the protein demand chain Protein is the building block of all life and essential for the growth of cells and tissue repair. The growing meat consumption triggers an increasing demand for animal husbandry and aquaculture which in turn require more animal feed and consequently sufficient crude protein. Unibio offers a unique alternative to existing sources of protein for animal feed by the use of an abundant raw material, methane.

Animal husbandry and aquaculture

Meat

Fish

Chicken

Compound feed

Pigs

Pork Eggs and dairy

Raw materials

Agriculture By-products

Fishmeal Pets

Cattle

Crude protein

Soybean meal

Fish Chicken

Beef

Animal feed

Fishing Other

Pet food UniProtein®

Rendering

Methane

Imagine a future where the world’s protein supply is partly covered by a technology enabling methane to be turned into protein by bacterial fermentation

8


Environmentally friendly production Components of the production process Main inputs

Methane

O2, phosphoric acid, ammonia

By-products Minimal use of water (which is also a by-product) 12 x output per hectare of arable land compared to soybean meal No need for pesticides

Clean water and limited CO2

The production of UniProtein® results in: Uniform quality Storage stability Regular supply at fixed prices

High % of crude protein and targeted amino acids Environmentally friendly product Modifiability to different animal groups

“The simulation results from a leading feed optimization software manufacturer showed that when UniProtein® was added by up to 5% of the total piglet feed weight, 6.5% less soybean meal was required. This alone resulted in a reduction of 250 kg CO2 eq. / tonne of feed. This equals to driving a car from Southern Denmark to Venice, Italy, for just one tonne of feed”. Lorie Hamelin, October 2015 Senior Scientist and PhD, Institute of Soil Science and Plant Cultivation, Department of Bioeconomy and Systems Analysis, Poland

9

uflnf s olo seeeedwsweerr Un iPUnriP ds otreot ienin®®22

dedss

SuSn

eesee

Ra

pReaps

WWh

BBaa rrlleey y aba eabena sn MMaa iizzee Gre OOaatt Peena ,pger ea PoP esn toatat otoee Ra pReas ss peese So edesd ySboe ya SuSnun benasn flo flo w seeerwseeer ds d WWhhe Un eaatt UiPnr iporot eteinin ®® Fab Fa

Global land use Land use varies significantly depending on the geographical location where the crops are cultivated. 1

baean nss

0

0

dry

1000 1000

SoS yobye

2000 2000

OOaa t PeaPe s, as

3000 3000

e

m3 / t

m2 / t

4000 4000

MMa iazize

5000 5000

3400 3400 3200 3200 3000 3000 2800 2800 2600 2600 2400 2400 2200 2200 2000 2000 1800 1800 1600 1600 1400 1400 1200 1200 1000 1000 800 800 600 600 400 400 200 200 0 0

BBa

6000 6000

arrleley y

Total water1 footprint of typically used ingredients in animal diets

heeat at

Land use1 of typically used feed ingredients in animal diets

Global total water consumption. The total water consumption (4.6 m³/t) is equal to blue water consumption during the production of UniProtein®. Total water consumption includes green, blue and grey water consumption. 1 2


Chemical composition Compound (dry matter - DM¹)

Mean values

Crude protein (N x 6.25)

72.9%

Crude fat

9.1%

Ash 550

8.6%

N-free extracts Nucleic acids

7.6%

Ribonucleic acid (RNA)

6.7%

UniProtein® has been shown to have a consistent chemical composition. After analysing different batches of UniProtein® data show that UniProtein® is a reliable commodity to be used as animal feed without raising issues of availability of agricultural land or dependence on weather conditions. The concentration of crude protein, lipids and ash of UniProtein® demonstrates small variations that can be attributable to differences in the growth media and growth rate.

Deoxyribonucleic acid (DNA) 2.3% Sugars (after acid digestion) Glucose Other monosaccharides Gross Energy, MJ/kg Compound (in DM)1

4.5% <0.5% 22.0 Mean values

Compound (in DM) 1

Amino acid composition

Mean values

Vitamins

Compound (in DM)1

Mean values

Cysteine Methionine

4.6 g/kg 19.4 g/kg

Vitamin A

<1 IU/g

Minerals Phosphorus

Thiamine B1

12.1mg/kg

Sulphur

5.4 g/kg

Threonine

32.3 g/kg

Riboflavin B2

73 mg/kg

Chloride

7.6 g/kg

Lysine Tryptophan

40.7 g/kg 18.9 g/kg

Nicotinic acid Inositol 30

130 mg/kg 30 mg/kg

Calcium Potassium

2.8 g/kg 6.9 g/kg

Leucine Isoleucine

54.5 g/kg 31.7 g/kg

<5 mg/kg Vitamin E Fatty acid composition

Magnesium Sodium

3.0 g/kg 7.0 g/kg

Valine Phenylalanine

42.2 g/kg 30.6 g/kg

Fatty acids in extracted fat

Iron Copper

332 mg/kg 96.0 mg/kg

Histidine

16.2 g/kg

Zinc

36.0 mg/kg

Arginine

44.5 g/kg

Relative distribution C14:0 Myristic acid

4.1%

Cobalt

3.4 mg/kg

Alanine Aspartic acid

51.3 g/kg 61.8 g/kg

C14:1

0.5%

Nickel

2.0 mg/kg

C15:0

0.7%

Manganese

<1.0 mg/kg

Glutamic acid Glycine

75.9 g/kg 36.3 g/kg

C16:0 Palmitic acid

49.3%

Selenium

0.017 mg/kg

Proline Serine Tyrosine Nitrogen components

28.6 g/kg 25.9 g/kg 23.9 g/kg

C17:00 Cyc17:0

6.0% 6.2%

C18:0 Stearic acid C18:1 Oleic acid Unidentified

0.3% 0.5% 2.3%

Cadmium Arsenic Lead

<0.00002 mg/kg 0.051 mg/kg 0.0023 mg/kg

Mercury

<0.02 mg/kg

Ammonium nitrogen

0.03 %

Amide

1.34 %

Nitrate Nitrite

<0.01 % <0.01 %

94.0%

19 g/kg

Values are an average of 13 batches of UniProtein® produced using pure M. capsulatus or a mixed culture. 1

DM = 94%

10


Seven reasons to use UniProtein® in animal diets 1. Competitive protein content The crude protein (CP) content of UniProtein® is superior to soybean meal (SBM) and comparable to fishmeal. Crude protein (% of DM) 80 80 70 70 60 60 50 50 40 40 30 30 20 20 10 10 00 Fishmeal Fishmeal

UniProtein®® UniProtein

Soybean meal Soybean meal(dehulled) (dehulled)

DM = Dry matter

The crude protein content of UniProtein® in comparison to fishmeal (60-68% CP as fed) or SBM. 2. Ideal amino acid profile UniProtein® has an ideal amino acid (AA) profile. UniProtein® has a higher concentration (% of CP) of valine, tryptophan, isoleucine and leucine compared to fishmeal. In the rest of the essential AAs there are minor differences between UniProtein® and average data for fishmeal and SBM. The content of AAs (% of CP) of UniProtein® in comparison with fishmeal and SBM 20 20 18 18 16 16 14 14 12 12 10 10 88 66 44 22 00

Ala Arg Asp Cys Glu Gly His Ala Arg Asp Cys Glu Gly His UniProtein®, CP 72.9 (% DM)

UniProtein , CP 72.9 (% DM) ®

Ile

Trp Lys1 Met Met Phe Phe Pro Pro Ser Ser Thr Thr Trp Ile Leu Leu Lys Tyr Val 1

Fishmeal, CP 70.6 (% DM)

Fishmeal, CP 70.6 (% DM)

SBM, CP 53.5 (% DM) (dehulled)

11

Val

Soybean meal, CP 53.5 (% DM) (dehulled)

AA = Amino acid CP = Crude protein DM = Dry matter SBM = Soybean meal 1 The low lysine content of UniProtein® can easily be addressed by adding feed-grade lysine to complete diets Source of values for fishmeal and SBM: http://www.feedipedia.org

Tyr


3. Low ash and mineral content The ash and mineral content of UniProtein®, particularly phosphorus and calcium, is low compared to fishmeal. This makes UniProtein® the ideal protein compound to incorporate into animal feed when a high protein content and a low mineral and ash content are required. Ash, calcium and phosphorus (% of DM) 2020 1818 1616 1414 1212 1010 88 66 44 22 00

Ash Ash

UniProtein® DM = Dry matter

Calcium Calcium Fishmeal UniProtein®

Phosphorus Phosphorus Fishmeal

The ash, calcium and phosphorus contents (% of DM) of UniProtein® in comparison with conventional-commodity fishmeal with a CP content of 70.6% of DM. Source of values for fishmeal and SBM: http://www.feedipedia.org

4. Valuable fatty acid profile The fatty acid profile of UniProtein® consists primarily of saturated C16:0 and monounsaturated C16:1 fatty acids representing approx. 85% of total fatty acids (TFA). Results of experiments indicate that the fatty acid profile of UniProtein® results in good oxidative stability of frozen pork and chicken meat.

6. Neutral flavour UniProtein® has a neutral taste. The palatability of diets including UniProtein® has been shown to be as well accepted as that of diets including typical protein sources.

5. UniProtein® is rich in B vitamins B vitamins are water-soluble and are not stored in the tissues in appreciable amounts. Their regular supply is essential.

7. Utilization and digestibility The digestibility of protein and AAs of UniProtein® diets and the retention of nitrogen (N) of animals on UniProtein® diets in the experiments have been comparable to the control diets. The main protein source in the control diets has been either fishmeal or SBM. 12


UniProtein® in pig diets Performance

The impact of increased inclusion of UniProtein® on pig performance Average daily gain1, g The average daily gain (ADG), average daily feed intake and feed conversion ratio (FCR) were similar among diets including UniProtein® from 0% to 10%.

900 900 800 800 700 700 600 600 500 500 400 400 300 300 200 200 100 1000 0

1

Increased levels of UniProtein® were balanced by a reduction of protein derived from SBM. Source: Øverland et al. 2004, Ital. J. Anim. Sci. 3:323-336

UniProtein® UniProtein 0% ® 0%

UniProtein® UniProtein 5% ® 5%

UniProtein® UniProtein 10%® 10%

from weanling (11.4 kg) to slaughter (107.2 kg)

Nutrient metabolism

Impact of increased inclusion of UniProtein® on the retention of nitrogen (N) and heat energy N Retention, g/kg 0.75 per day 1.5 1,5

Heat energy, kJ/kg 0.75 per day 800 800

600 600

1.0 1,0

400 400

0.5 0,5 200 200

0.0 0,0

® UniProtein UniProtein® 0% 0% N = Nitrogen

® UniProtein UniProtein® 5% 5%

® ® UniProtein UniProtein UniProtein® UniProtein® 15% 10% 10% 15%

00

® UniProtein UniProtein® 0% 0%

® UniProtein UniProtein® 5% 5%

® ® UniProtein UniProtein UniProtein® UniProtein® 15% 10% 10% 15%

UniProtein® incorporated by up to 15% into pig diets maintained the good N retention and showed no adverse effects on heat energy. Increased levels of UniProtein® were balanced by a reduction of protein derived from SBM. Source: Hellwing et al. 2007, Animal 1:45-54

13


UniProtein® in pig diets Meat quality

The impact of increased inclusion of UniProtein® on the quality of frozen pork Rancid taste1

PUFA, % of TFA in backfat 20 20 18 18 16 16 14 14 12 12 10 10 88 66 44 22 00

1.6 1,6 1.5 1,5 1.4 1,4 1.3 1,3 1.2 1,2 1.1 1,1 ® UniProtein UniProtein 0% 0%

11

® ® UniProtein UniProtein 10% UniProtein UniProtein 15% 15% 10%

® UniProtein UniProtein 5% 5%

®

®

®

®

PUFA = Polyunsaturated fatty acids TFA = Total fatty acids

1

® UniProtein UniProtein® 0% 0%

UniProtein ® UniProtein 5%

®

5%

UniProtein ® UniProtein 10%

®

10%

UniProtein® 15%

UniProtein® 15%

On a scale from 1 (poorest) to 9 (best)

Increased levels of UniProtein® in the diets decreased the ratio of polyunsaturated fatty acids (PUFA) to total fatty acids (TFA) in backfat and led to better oxidative stability of frozen pork as shown by the improved rancid taste. Increased levels of UniProtein® were balanced by a reduction of protein derived from SBM. Source: Øverland et al. 2005, Meat Sci. 71:719–729

The increased levels of UniProtein® in the diets improved fat firmness in a linear manner. Increased levels of UniProtein® were balanced by a reduction of protein derived from SBM.

Fat firmness1 15 15 14 14

Source: Øverland et al. 2004, Ital. J. Anim. Sci. 3:323-336

13 13 12 12 11 11 10 10

UniProtein ® UniProtein 0%

®

0%

1

® UniProtein UniProtein 5%

®

5%

UniProtein ® ® UniProtein UniProtein 10% UniProtein 15%

®

®

10%

15%

On a scale from 1 (poorest) to 9 (best)

14


UniProtein® in broiler diets Performance

The impact of increased inclusion of UniProtein® on broiler performance when replacing SBM Body weight1, g

FCR1, g feed/g weight gain

2500 2500

1,6 1.6

2000 2000

1.5 1,5

1500 1500

1.4 1,4

1000 1000

1.3 1,3

500

1.2 1,2

500

0

0

1

1.1 UniProtein® UniProtein 0%

®

0%

day 32

UniProtein® UniProtein 4%

®

4%

UniProtein ® UniProtein 8% 8%

®

UniProtein ® UniProtein 12%

1,1

®

UniProtein®® UniProtein 0% 0%

12%

UniProtein®® UniProtein 4% 4%

FCR = Feed conversion ratio 1 day 0 - day 32

® UniProtein® UniProtein ® UniProtein 12%

® UniProtein 8% 8%

12%

The body weight and feed intake were similar among the diets including UniProtein® from 0% to 12%. The FCR was statistically similar among the diets. However, UniProtein® inclusion resulted in the numerical improvement of FCR compared to the control diet. Source: Øverland et al. 2010, Br. Poult. Sci. 51:686-695

Digestibility The impact of increased inclusion of UniProtein® on the amino acid digestibility of diets when replacing SBM

1.01,0 0.90,9 0.80,8 0.70,7 0.60,6 0.50,5 0.40,4 0.30,3 0.20,2 0.10,1 00,0

Coefficients of AID of essential AAs in diets1 The apparent ileal digestibilities (AID) of amino acids were unaffected or improved when UniProtein® replaced SBM in the diets. Source: Schøyen et al. 2007, Arch. Anim. Nutr. 61:276-291 Arg

His

Ile Ile

UniProtein®

® UniProtein 0% 0%

Leu Leu

UniProtein®

® UniProtein 2% 2%

AA = Amino acid AID = Apparent ileal digestibility 1 Measured at day 32 15

Lys Lys

Met Met

Phe Phe

UniProtein®® UniProtein 4% 4%

Thr Thr

Trp Trp

Val Val

UniProtein®® UniProtein 6% 6%


UniProtein® in broiler diets Meat quality

The impact of increased inclusion of UniProtein® on the quality of chicken meat when replacing SBM PUFA, % of TFA

Oxidative products1

45 45

0.035 0,035

44 44

0.03 0,03

43 43

0.025 0,025

42 42

0.02 0,02

41 41

0.015 0,015

40 40

0.01 0,01

39 39

0.005 0,005

38 38

UniProtein UniProtein UniProtein 0% ® 0% UniProtein 4% ® 4% ®

®

UniProtein

®

00

UniProtein

®

PUFA = Polyunsaturated fatty acids TFA = Total fatty acids

UniProtein®

UniProtein® UniProtein 4% ® 4%

UniProtein 0% ® 0%

UniProtein 12%® 12% 8% ® 8% UniProtein

Butyraldehyde2 Butyraldehyde

2

UniProtein® UniProtein® UniProtein 12%® 12%

UniProtein 8% ® 8%

Hexanaldehyde3 3 Heptanaldehyde3 Hexanaldehyde Heptanaldehyde3

in the thigh meat after 6 months of storage at −18°C µg/g ×10 3 µg/g 1 2

UniProtein® decreased the ratio of PUFA to TFA in chicken fat, reduced the level of oxidative products in frozen meat and had no adverse effects on the several sensory quality parameters related to odour and flavour. Source: Øverland et al. 2011, Poult. Sci. 90:201-210.

16


UniProtein® in broiler diets Performance The impact of increased inclusion of UniProtein® on broiler performance when replacing fishmeal Body weight1, g

FCR1, kg feed/kg body weight gain

2000 2000

22

1500 1500

1,5 1.5

1000 1000

11

500 500

0.5 0,5

00

1

UniProtein® ® UniProtein®® UniProtein® ® UniProtein®® UniProtein 0% 0% UniProtein 2% 2% UniProtein 4% 4% UniProtein 6% 6%

day 32

00

® UniProtein®® UniProtein®® UniProtein® ® UniProtein ® UniProtein 0% 0% UniProtein 2% 2% UniProtein 4% 4% UniProtein 6% 6%

FCR = Feed conversion ratio day 0 - day 32

1

Body weight, feed intake and FCR were similar among the diets including UniProtein® from 0% to 6%. Source: Schøyen et al. 2007, Arch. Anim. Nutr. 61:276-291

Digestibility

The impact of increased inclusion of UniProtein® on the amino acid digestibility of diets when replacing fishmeal Coefficients of AID of essential AAs in the diets1 1.0 1,0 0.9 0,9 0.8 0,8

The AIDs of amino acids were unaffected when UniProtein® replaced fishmeal in the diets.

0.7 0,7 0.6 0,6 0.5 0,5 0.4 0,4 0.3 0,3 0.2 0,2 0.1 0,1 0 0,0 Arg His Ile Leu Lys Met Phe Thr Trp Val Arg His ®Ile Leu Lys Met Phe Thr Trp Val UniProtein UniProtein® UniProtein® UniProtein® 2% ® 4% ® 6% ® ® UniProtein0% UniProtein UniProtein UniProtein 0% 2% 4% 6% AID = Apparent ileal digestibility AA = Amino acid 1 Measured at day 32 17

Source: Schøyen et al. 2007, Arch. Anim. Nutr. 61:276-291


UniProtein® in broiler diets Nutrient metabolism The impact of increased inclusion of UniProtein® on the protein and energy metabolism N retention, g /kg 0.75 per day

Heat energy, kJ/kg 0.75 per day

1.7 1,7

1000 1000

1.6 1,6

800 800

1.5 1,5 1.4 1,4

600 600

1.3 1,3

400 400

1.2 1,2

200 200

1.1 1,1 1.0 1,0

® UniProtein® 0% UniProtein

0%

® UniProtein® 2% UniProtein

2%

® UniProtein® 4% UniProtein

4%

® ® UniProtein 6% UniProtein

6%

00

UniProtein®®0% UniProtein UniProtein®®2% UniProtein UniProtein®®4% UniProtein UniProtein®®6% UniProtein 0% 2% 4% 6%

N = Nitrogen

The values of retained N and heat energy were similar among the diets including UniProtein® from 0% to 6% of diet. Increased levels of UniProtein® were balanced by a reduction of protein derived from fishmeal. Source: Hellwing et al. 2006, Arch. Anim. Nutr. 60:365-281

18


UniProtein® in broiler diets Meat quality

The impact of increased inclusion of UniProtein® on the quality of chicken meat Juiciness1,2

Off-odour and off-flavour1,2 44

55

33

44

22

33

11

22

00

11 ® ® ® ® ® ® ® ® UniProtein 0% UniProtein 2% UniProtein 4% UniProtein 6% UniProtein UniProtein UniProtein UniProtein

0%

Off-odour 1 2

2%

Off odour

4%

Off flavour

® UniProtein®®0% UniProtein UniProtein® ®2% UniProtein UniProtein® ® 4% UniProtein UniProtein® 6% UniProtein 0% 2% 4% 6%

6%

Off-flavour

On a scale from 1 (no intensity) to 9 (high intensity) 175 freeze-stored days

1 2

On a scale from 1 (no intensity) to 9 (high intensity) 175 freeze-stored days

Replacing fishmeal by UniProtein® in the diet improved the sensory quality of frozen-stored chicken meat as shown by the less intense off-odour and off-flavour and increased the juiciness of frozen meat. The off-odour and off-flavour decreased, and juiciness increased numerically when UniProtein® replaced SBM in the diet. Source: Schøyen et al. 2007, Arch. Anim. Nutr. 61:276-291

19


UniProtein® in mink diets Performance and fur quality

The impact of increased inclusion of UniProtein® on mink performance Total impression of fur quality characteristics1

Body weight gain1, g 2500 2500

77

2000 2000

66 55

1500 1500

44

1000 1000

33

500 500

22

0

11

0

UniProtein®® 0% UniProtein 0%

UniProtein® 4% UniProtein 4% ®

UniProtein ® 8% UniProtein 8% ®

® ® UniProtein 0% UniProtein

0%

Males 1

from 29 June to 26 November (a total of 121 feeding days)

The body weight gain, feed intake and FCR were similar among diets including UniProtein® by 0% to 8%. Increased levels of UniProtein® were balanced by a reduction of protein derived from fishmeal. Source: Ahlstrøm et al. 2006, J. Anim. Feed Sci. 15:491-504

1

®® UniProtein 4% UniProtein

4%

®® UniProtein 8% UniProtein

8%

Females

On a scale from 1 (poorest) to 9 (best)

UniProtein® incorporated by up to 8% in the diets improved the total impression of fur quality characteristics compared with the control diet. Increased levels of UniProtein® were balanced by a reduction in protein derived from fishmeal. Source: Ahlstrøm et al. 2006, J. Anim. Feed Sci. 15:491-504

20


UniProtein® in mink diets Nutrient metabolism and digestibility

The impact of increased inclusion of UniProtein® on N retention and the digestibility of N and AAs in the diet

N retention, g/d

N digestibility, %

1.4 1,4

100 100

1.2 1,2

80 80

1.0 1,0 0.8 0,8

60 60

0.6 0,6

40 40

0.4 0,4

20 20

0.2 0,2 0 0,0

® UniProtein UniProtein 0%

® UniProtein UniProtein 4%

®

0%

00

® UniProtein UniProtein 8%

®

®

4%

8%

® UniProtein UniProtein® 0% 0%

® UniProtein UniProtein® 4% 4%

® UniProtein UniProtein® 8% 8%

N = Nitrogen

UniProtein® incorporated by up to 8% into male mink diets maintained the good retention and digestibility of N. Increased levels of UniProtein® were balanced by a reduction in protein derived from fishmeal. Source: Ahlstrøm et al. 2006, J. Anim. Feed Sci. 15:491-504

Coefficients of ATTD of AAs in the diet 1.0 1,0 0.9 0,9 0.8 0,8 0.7 0,7

UniProtein® incorporated by up to 14.7% into mink diets maintained the good apparent total tract digestibility (ATTD) of main nutrients and AAs. Increased levels of UniProtein® were balanced by a reduction in protein derived from fishmeal.

0,6 0.6 0.5 0,5 0.4 0,4 0.3 0,3 0,2 0.2 0.1 0,1 0 0,0

Source: Øverland et al. 2006, Anim. Feed Sci. Tech. 129:237-251 Arg Arg

His His

Ile Ile

UniProtein® 0%0% UniProtein®

Leu Leu

Lys Lys

Phe Phe

Thr Thr

Trp Trp

UniProtein® UniProtein® 14.7% 7.35% UniProtein® 7.35% UniProtein® 14.7%

AA = Amino acid ATTD = Apparent total tract digestibility

21

Met Met

Val Val


UniProtein® in blue fox diets Nutrient metabolism and digestibility

The impact of increased inclusion of UniProtein® on the production performance and diet digestibility in blue foxes.

ATTD of main nutrients in the diet, %

Final body weight1, kg

100

1212

80

1010 88

60

66

40

44

20

22

0

00 UniProtein 0%

®

UniProtein 4%

®

UniProtein 8%

®

UniProtein 12%

The ATTD of crude protein The ATTD of crude fat The ATTD of crude carbohydrate ATTD = Apparent total tract digestibility

® ® ® ® UniProtein UniProtein 0% UniProtein UniProtein 4% UniProtein UniProtein 8% UniProtein UniProtein 12% 0% 4% 8% 12%

®

®

1

®

®

®

at 5 December

Final body weight, overall gain and feed consumption were similar among diets including UniProtein® by 0% to 12%.

The ATTDs of main nutrients were similar among the diets including UniProtein® from 0% to 12%. Source: Skrede and Ahlstrøm 2002, J. Nutr. 132: 1668S–1669S

The study was conducted to investigate the potential use of UniProtein® in diets of dogs using blue foxes as a model.

22


UniProtein® in aqua diets Performance

The impact of increased inclusion of UniProtein® on the performance of Atlantic salmon when replacing fishmeal Final weight1, g

Final weight1, g 44

5050 4040

33

3030

22

2020

11

1010

00 ® ® ® UniProtein UniProtein UniProtein® 0% UniProtein UniProtein® 5% UniProtein UniProtein® 9.9% UniProtein 19.3% 9.9% 19.3% 0% 5% ®

1

®

day 112

00 ® UniProtein UniProtein® 0% 0% 1

® UniProtein UniProtein® 5% 5%

UniProtein UniProtein ® ® UniProtein 9.9% UniProtein 19.3% ®

9.9%

®

19.3%

day 364

In a 12-month experiment the salmon (at age of 112 days) fed the diet containing 5% UniProtein® weighed significantly more than salmon fed the fishmeal-based control diet, while salmon fed the diets containing more UniProtein® reached the same weight as the control group. The salmon (at age of 364 days) fed the diet containing 19.3% UniProtein® ended up with a higher final weight than salmon fed the fishmeal-based control diet or the diets including less UniProtein®. Specific growth rate and survival of fish fed up to 19.3% UniProtein® were similar among diets during the entire experiment. Source: Storebakken et al. 2004, Aquaculture, 241:413-425

Body weight1, g 370 370

UniProtein® improved the body weight of juvenile Atlantic salmon.

360 360 350 350

Source: Aas et al. 2006a, Aquaculture, 259:365-376

340 340 330 330 320 320 310 310 300 300

1

® UniProtein UniProtein® UniProtein® UniProtein® UniProtein® UniProtein® UniProtein® UniProtein® UniProtein® UniProtein® 0% 4.5% 9% 18% 0% 4.5% 9% 18% 27%27%

day 52 23


UniProtein® in aqua diets Performance

The impact of increased inclusion of UniProtein® on the performance of rainbow trout when replacing fishmeal FCR1, g weight gain/g feed

Specific growth rate1 1.41 1,41

1,2

1.40 1,40 1.39 1,39 1.38 1,38

1,1

1.37 1,37 1.36 1,36 1,0

1

® UniProtein UniProtein® 0% 0%

® UniProtein UniProtein® 9% 9%

® UniProtein UniProtein® 18% 18%

day 76

1.35 1,35

® UniProtein UniProtein 0%

®

0%

® UniProtein UniProtein 9%

®

9%

® UniProtein UniProtein 18%

®

18%

FCR = feed conversion ratio 1 day 0 – day 76

The specific growth rate and feed intake of trout were similar among diets including UniProtein® from 0% to 18%. The FCR was similar between the control diet and the diet including 18% UniProtein®, while the FCR of trout on a diet including 9% UniProtein® outperformed the FCR of trout on the control diet or the diet containing 18% UniProtein®. Source: Aas et al. 2006b, Aquaculture, 261:357-368

24


UniProtein® in aqua diets Nutrient metabolism and digestibility

The impact of increased inclusion of UniProtein® on the diet digestibility of rainbow trout 100 100 90 90 80 80 70 70 60 60 50 50 40 40 30 30 20 20 10 10 0 0

AID of N, %

® UniProtein UniProtein® 0% 0%

® UniProtein UniProtein® 9% 9%

UniProtein ® UniProtein 18% ®

18%

AID = Apparent ileal digestibility N = Nitrogen

100 100 90 90 80 80 70 70 60 60 50 50 40 40 30 30 20 20 10 10 0 0

AID of energy, %

® UniProtein UniProtein® 0% 0%

® UniProtein UniProtein® 9% 9%

UniProtein ® UniProtein 18% ®

18%

AID = Apparent ileal digestibility

N retention (% digested) 60 60

The AIDs of N, energy and AAs in rainbow trout differed slightly among diets including UniProtein® by 0% to 18%.

50 50 40 40

The N retention in rainbow trout were similar among diets including UniProtein® by 0% to 18%. Increased levels of UniProtein® were balanced by a reduction in content of protein from fishmeal.

30 30 20 20 10 10 00

Source: Aas et al. 2006b, Aquaculture, 261:357-368 ® UniProtein UniProtein® 0% 0%

N = Nitrogen

25

® UniProtein UniProtein® 9% 9%

UniProtein ® UniProtein 18% ®

18%


The proper use of UniProtein® in animal diets Maximum inclusion levels of UniProtein® in animal diets based on initial studies Species

Inclusion level (% of diet)

Broiler

6

Laying hen1

3

Rainbow trout

20

Atlantic salmon

20

Pig2

8

Mink

8

Blue fox

12

Dog and cat3

12

Based on trials in broilers and considering the low protein content of layers’ diets compared to the diets of broilers Based on trials conducted in pigs from approx. 10 kg to 80 – 110 kg 3 Based on trial in blue foxes 1 2

26


Turning natural gas into food

Copenhagen Office

Unibio International plc.

Unibio

Unibio

2 Royal College Street London NW1 ONH United Kingdom

Diplomvej 381 DK-2800 Kgs. Lyngby Denmark

Niels Bohrs Alle 17 DK-5230 Odense M Denmark

+44 (0) 20 7691 4902 unibio@unibiogroup.com

+45 25 75 18 10 unibio@unibio.com

+45 63 10 40 40 unibio@unibio.com

www.unibiogroup.com

Š Unibio A/S, DK 2017

Administrative Headquarters

Global Headquarters


UniProtein product catalogue