ENVIRONMENTAL

Biological Pond Treatment

AquaGrow™ & AquaFlow™

Feed and Water Treatments

OTHERS…

Septic Treatment

Household Treatments

ProbioPond’s™ biologically active formula contains a proprietary blend of bacterial strains that were selected for the ability to breakdown excess waste material in ponds and reduce the amount of available phosphorous causing a reduction in the algal population.

ProbioPond’s™ naturally occurring cultures efficiently digest pond bottom materials and reduce the organic matter in the water column leading to cleaner water and higher oxygen levels.

The bacteria are stabilized and shipped in spore form, ensuring long term stability and extended shelf life.

As demonstrated in the graph, the application of ProbioPond resulted in a significant reduction in Chlorophyll A, a measure of algal concentration, Phosphorous, and turbidity.

These results were not generated in a laboratory, rather, they were obtained under actual field conditions.

AquaGrow™ is a biologically active formula of our bacterial strains that were chosen because of their ability to rapidly digest excess waste material in shrimp ponds. The scientifically selected, proprietary blend of cultures colonize the animal gut to aid in feed digestion and help protect shrimp and fish against pathogens.

As shown in the chart above, using AquaGrow equates to an increase in survival and weight of the animal, as well as a higher payback ratio. AquaGrow’s cultures were chosen for optimum enzyme production, assuring efficient breakdown of proteins, carbohydrates, cellulose and fats. This leads to better feed conversion and less waste material for cleaner water and higher oxygen levels. Additionally, harmful ammonia and nitrates subside for healthier aquatic animals.

Industrial Treatments

MicroSynergies LLC is the world leader in sourcing for the largest collection of commercial microbial ingredients on the planet. Through our agreements, partnerships, and network of global microbial manufacturers, we connect our customers to thousands of strains in formats including bulk cultures, concentrates, contract fermentations, and specialty biologicals and market them under our proprietary brand names. In many of these formats, we’re able to include our value-added MicroSynergy™ technology for creating enhanced stability, longer shelf-life, and increased activity post-production. From there, we can augment our offerings with expert formulation and technical support, market access, our award-winning 3+80™ supply risk guarantee, and microbial-specific marketing expertise. All information and data supplemented by data and visuals are independently verified, current, and correct to the best of our knowledge. For more information, contact your MicroSynergies representative.

To learn more, visit ContractFermentation.com reat probiotic manufacturers spend both years and plenty of money in researching and developing their proprietary in production probiotic cultures and strains. But sometimes the best product requires the use of a highly specific strain— either for regulatory reasons, label claims and efficacy, or both. This is where custom contract fermentation comes into play.

With custom contract fermentation, leading bulk culture manufacturers can use their existing knowledge and infrastructure to setup fermentations of strain-specific cultures for companies seeking to further differentiate their biological-based products in the marketplace.

Choosing Custom Fermentation

The decision to go down the path of custom fermenting of your specific strain may seem straightforward but there some factors to consider.

To get right to the point, custom fermentation is usually more expensive— especially at the onset— than choosing an inproduction culture of the same species. This is because, generally speaking, a production schedule will need to be adjusted for your specific fermentation and additional lab analysis performed to insure optimal growth and stability.

Yet, as the volume of fermentation orders for the custom strain continues to rise and the market sees your product as something that no one else has or can get access to, the benefits may outweigh the costs.

In the case that there may be no clear

Gbenefit to a custom strain, choosing a strain that’s already in production may be the best choice— especially if the product is a brand-new concept and needs market vetting before it scales up for your company.

Choosing A Manufacturing Partner

A myriad factors can come into play when vetting a contract fermentation partner. These can include geographic location, facility grade, experience with a given organism, total capacity, scalability, BSL level, certifications, market access, stability technologies, reputation, ownership of media formulations, and of course price.

Pricing Contract Strain Fermentations

Just as in-production microbial cultures can vary widely (see Why Microbial Prices Can Vary Widely in this issue) so can contract fermentations. The reasons for this can be many, but it usually comes down to a couple of key factors: how the resulting culture is priced and the level of comfort and confidence of the manufacturer in their ability to scale the strain to production.

Some manufacturers will price a contract fermentation based simply on the size of the fermenter used (e.g. priced as one 5,000L tank), yield (the number of kilograms of culture that result from the fermentation), concentration (the density of live and active cells per gram or mL), or the time it takes up in lab analysis and production — or a combination of these factors.

In addition, the manufacturer’s level of familiarity with the strain is important. For example: if the manufacturer already grows a production strain of Bifidobacterium longum, then your unique strain of B. longum likely shares some characteristics that they can use to more efficiently and effectively get the most out of your strain… and of course the inverse is true too—

Chris Muellenbach Vice President of Marketing and Business Development MicroSynergies

Brad Glocke President MicroSynergies

Minimum Requirements For Strain Fermentation Strain

If provided by customer, third party typing (identification) has been completed. For BSL2 organisms, safety data has been approved by the highest regulatory body in the locale of the fermentation partner.

If provided by MicroSynergies, the organism must be BSL1 for contract fermentation or up to BSL3 for research procurement.

R&D WORK

For most genera (see chart to the right), we have the ability to optimize media. For adjacent genera (e.g. Paenibacillus to Bacillus), media optimization pre-work must be nearly complete.

Project Commitment

An MOQ of a 5,000L tank (we have the ability to scale-up from there) with longer-term commitment pending the project’s commercial success.

and would likely be priced in terms of increased R&D costs. The fact is that just like any other business, fermentation companies will price a custom run based on what it costs them to analyze, scale, and grow a strain, not necessarily what the going market rate is. There is nothing wrong with this approach, but it can make for some large discrepancies in pricing on a per-CFU basis.

Navigating The Process

Because we work with a portfolio of capable manufacturers around the world, we are able to offer near unbiased guidance on options for the direction of your custom strain fermentation. Feel free to reach out and start a conversation to see how our team can help yours.

CURRENT CONTRACT FERMENTATION CAPABILITIES BY GENUS

Bacillus HU, NH

Bifidobacterium HU

Bradyrhizobium NH

Enterococcus HU

Escherichia HU, NH

Lactobacillus HU

Lactococcus HU

Pediococcus HU

Propionibacterium HU

Pseudomonas NH

Rhizobium NH

Streptococcus HU

To learn more, visit ContractFermentation.com

Contract Fermentation Considerations

MicroSynergies has helped companies find capable fermentation partners and navigate the process from strain sourcing, R&D analysis, media formulation, and commercial scale-up. We help our you navigate this process with our Contract Fermentation Information and Questionnaire, which includes guidance on the following— and more:

Strain Sourcing

For when customers don’t have their own strain, we have helped customers source strains from natural isolate programs, universities, and institutions.

MAXIMIZING YIELD & CONCENTRATION

Using in-house working species models and in conjunction with market viability, we help determine where maximum yield and concentration should be so that any pre-work can be maximized before transitioning the project to a commercial setting.

Pricing Landscape

We help companies understand pricing structures and guide them towards the one that’s best for their business for the lifetime of the product.

Format

From powders to liquids— and even including finished products, we’ve helped companies get the most out of the contract fermentation process.

Regulatory

Navigating regulations and international import and export can be challenging, but we’ve been up to the task for years.

Marketing Support

Let us help you craft a campaign with robust design and expert technical claim guidance.

MicroSynergies

3+80 Guarantee™

Establishing New Links in the Chain MicroSynergies 3+80™

Guarantee at risk of not being able to be fulfilled in its entirety due to national freeze-drying companies being tasked with switching from processing peripheral products like microbial cultures to solely focusing on end-use foods like fruits and vegetables in the wake of initial COVID-19 food shortage risks.

MILWAUKEE -- The Metropolitan Milwaukee Association of Commerce (MMAC) is recognizing Milwaukee Region organizations as Focus on the Future Award honorees for their work to meet challenges presented by the COVID-19 pandemic.

“Our business community has faced unprecedented challenges in 2020,” said Stephanie Hall, MMAC Vice President…

“Through it all, these 65 companies, organizations and individuals have shown us examples of the Milwaukee Region at its best, and give us hope for the future. Through the Focus on the Future Awards, MMAC wants to recognize those who have risen to their best during the most difficult of times.”

Established New Links In The Chain

These honorees have retooled their supply chain or made new links in another supply chain to meet new market demands: MicroSynergies LLC

MICROSYNERGIES 3+80 GUARANTEE

In order to protect our customers’ businesses and reduce their supplychain risk, MicroSynergies is committed to having the top 80% of our microbes available in three-deep backup supply.

In summer of 2020, a large order from a Wisconsin company was

Despite this external business disruption, MicroSynergies was able to use our network to locate the strain needed, get it into production right away, and hand-deliver the culture on-time and to our customer’s specifications.

“MicroSynergies provides great customer service and always comes through with our microbial culture needs. We really appreciate the hard work and dedication to keep our business running smoothly.”

— Alex (Wisconsin), Vice President of potentially affected company

Prior to the potential challenges that this scenario might have presented, MicroSynergies was already in the process of establishing our 3+80 Guarantee, a proactive mapping of our network of in-production and available microbial cultures in order to know that at any given time we will have 3 sources for the top 80% of our microbial cultures. This is our “3+80 Guarantee.”

To learn more, visit MicroSynergies.com/Guarantee

Chris Muellenbach Vice President of Marketing and Business Development MicroSynergies

Creating unique and competitive products that serve a local market need is exciting— but it’s already a big enough challenge as it is. When it comes time to enhance your offering by additing certifications or expanding into new markets, the resulting challenges can sometimes seem overwhelming and sometime even too much work to pursue.

Like we’ve often joked with our customers, “If it requires this much patience, just think of how difficult it will be for other products to compete with yours.” And for better or worse, the intricacies of various regulatory processes often make this true. Yet, we aim to make the process as seamless and efficient for our customers as possible by working with importers, exporters, and regulators in the background to get your product approved.

WHERE ARE THE HURDLES?

The short answer is: it varies. For example, for a given species of bacteria, there may be a national regulatory body and a sub-national one (in the U.S., think: the USDA vs. AAFCO— or in Canada: the CFIA vs. Health Canada)— and these may be in combination with national sub-regulators and local ones (again, in the U.S. think: OMRI vs. MOSA).

Then, when it comes to transplanting a product into a different international location, there may be different regulations on (A) the species of organisms that can be utilized in the first place (e.g. Canada), how they can be grown and processed (e.g. Australia), or even approvals on a particular strain level (e.g. the EU/EAA). At this point, you’ve either “been there” or you haven’t (regardless, I won’t continue to bore you with more examples); but either way we can help.

FRAMING PRODUCT EXPANSIONS & OPTIONS

MicroSynergies can and has helped companies create products in dozens of industries and in dozens of countries (see Global Market Access in this volume). Between our in-house experience and that of our network of partners, we have the capability of working with many different import, export, and regulatory bodies. Below is a sampling of these— yet, we’re confident in our abilities to help our customers through the regulatory process in many other environments as well. Contact us today and we’ll be happy to get started on your next product expansion!

United States

USDA, EPA, AAFCO, FDA, OMRI, NOP, MOSA, NICS, PCO, GOA, CDFA, etc.

Canada

Health Canada (and List C), CFIA, FDA (and ICL, R-ICL, DSL), RCC (and TSCA, NDSL)

Europe

EFSA (and QPS), EC/REACH (and ECHA)

Australia

DAWR, AQIS (and BICON)