Understand A Compound’s ADME Properties With In Vitro ADME/PK Screening

Understand A Compound’s ADME Properties With In Vitro ADME/PK Screening

A drug undergoes many changes as it moves through the human body. Studying how a drug moves and changes through the process of absorption, distribution, metabolization, and elimination (ADME) gives researchers the ability to compare their drug’s ADME data against a library of drugs with known ADME parameters. Through this comparison, researchers can identify if their drug has potential liabilities due to poor ADME characteristics. In drug discovery, the earlier potential drug liabilities are identified the more time and money is saved on more costly clinical trials. As unforeseen ADME qualities can be a major cause for drug failure, in the clinic, understanding these characteristics early is paramount.

In vitro ADME studies are an essential panel of screens used to de-risk drug candidates early. By understanding ADME properties with less costly cell based in vitro assays a researcher can make go no go decisions before entering costly in vivo studies or experiencing failure in the clinic. In vitro studies work by describing the potential impact a drug may have on a living system within the context of cellular biology and biochemistry. When done correctly in vitro ADME combined with toxicity studies provide precise information on the metabolism and safety profile of a drug candidate, enabling higher chances of success in clinical trials by de-risking a compound of characteristics known to cause problems in humans .

In the past, researchers relied heavily on animal testing to gain ADME data. Now, primary cells derived from human tissue can be utilized as alternatives to animal models. It is important to conduct these studies in a controlled environment, which is why researchers depend on their deep industry knowledge and FDA approved standards for the In Vitro ADME studies.

As scrutiny on the choice of animal models increases and in vitro models continue to advance there is greater acceptance of in vitro assays for early screens. For example, primary human hepatocytes separated from the liver are now accepted as a predictive in vitro model for drug metabolism and the US FDA recommends running a number of in vitro ADME assays prior to applying for an Investigational New Drug (IND).

Gaining In Vitro ADME data as early as possible in the drug discovery program has many advantages by helping avoid costly failures in the clinic. Therefore, selecting a good Contract Research Organization is then very important.

Selecting a Contract Research Organization (CRO) to Perform In Vitro ADME/PK Screening

IONTOX is an in vitro Contract Research Organization which follows good cell culture and laboratory practices and employs some of the world's top in vitro scientists. The company's focus is on providing high quality data and offers a number of gold standard in vitro ADME assays to support drug discovery programs.

Some of the core ADME assays offered by IONTOX are as follows: -Caco-2 Permeability -Metabolic Stability -CYP Induction -CYP Inhibition -Plasma Protein Binding -Blood Partitioning -Evaluation of Transporter Interactions

To learn more about In vitro ADME, please visit https://www.iontox.com/in-vitro-adme-pktesting/ today.