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Field-tested health protection for Shrimp feed by Tilman Wilke 1, Monika Korzekwa1 and Niti Chuchird2 1 Dr. Eckel GmbH, Niederzissen, Germany 2 Aquaculture Business Research Center, Kasetsart University, Bangkok, Thailand


r. Eckel – Germany’s pioneer in functional feed additives – is constantly working on new solutions to provide reliable solutions for premium aquaculture feed. Dr. Eckel now releases Anta®Ox Aqua – the all-natural feed additive for intensive Shrimp production. Shrimp farmers from around the world have the same goal to keep their production system in a balance. Endemic pathogens and epidemic diseases (like EMS/AHPN caused by Vibrio parahaemolyticus) constantly put this balance at risk. In the past it was commonplace to keep farming system in balance by the preventive use of antibiotics. Customers and retailers in Europe, the US and other markets become more and more sensitive to the abundance of antibiotic drugs usage in animal production. Hence, shrimp farmers need alternative tools to keep their animals healthy. Functional feed additives are alternative way to strengthen the natural defence mechanism of the animals and therefore to keep the production system in balance. The farmer recognizes this by high growth rates, good feed conversion and low mortalities.

Lab results reconfirmed in large-scale field trials

Anta®Ox Aqua can directly decrease the bacterial infection and increase the survival rate of Pacific White Shrimp. This was confirmed in feeding trial in the lab as well as in the pond. Trials conducted in cooperation with the renowned Kasetsart University in Thailand gave spectacular results under laboratory conditions. The protective and immune-stimulating effects of Anta®Ox Aqua at the cellular level were measured and visualized (Niyamosatha et al. 2015). In follow-up pond trials conducted under supervision of Kasetsart University of Bangkok these results have now been confirmed under commercial farming conditions in South-East Asia. Anta®Ox Aqua significantly reduced the number of Vibrio

bacteria in the hepatopancreas (Fig. 1). EMS is known to cause necrotic cells in the hepatopancreas of shrimp. In samples of the control group up to 80 percent of the hepatopancreatic cells were consistently damaged. However, the hepatopancreas of the shrimps with Anta®Ox Aqua appeared to be healthy and normal (Fig. 2). Furthermore, the lower bacterial infection in Anta®Ox Aqua fed shrimps accounted for better mean survival rate (91% vs. 63% in the control group, Fig. 3). Performance parameters like growth and feed conversion ratio (1.35 vs. 1.55 in the control group) were positively affected by adding Anta®Ox Aqua to the growout feed (Fig. 4). These effects increased total biomass production and profit in the Anta®Ox Aqua group. In addition to that, lower mortality in the grow out period means less waste in terms of animals and feed and enables farmers to plan annual production volumes more reliably.

The mode of action of Anta®Ox Aqua

Inflammation is a reaction to damage to animal tissues (whether visible or invisible) caused by various stimuli. Those stimuli can be physical (e.g. injuries, heat and radiation) chemical (e.g. acids and toxins) and biological (e.g. bacteria, fungi and viruses). Numerous of these factors can be found in aquaculture production, which means that undetected inflammation is likely to be present almost continuously. The inflammatory process is controlled and activated by the transcription factor “Nuclear Factor kappa B” (NF-κB). After stimulation by various inducers, NF-κB activates the expression of genes encoding pro-inflammatory proteins or enzymes involved in the inflammatory process. The inflammatory mediators contribute to the disruption of epithelial barriers and activate other immune cells which enhance the inflammation of the digestive system. Inflammation in the digestive system and adjacent organs costs energy, reduces growth performance and impairs feed utilization of aquaculture species. The active ingredients in Anta®Ox Aqua act as natural antiphlogistics by down-regulation of the NF-κB factor and

22 | January | February 2016 - International Aquafeed

Jan | Feb 16 - International Aquafeed magazine