PRESENTATION BY POLISH GROUP
ARE ALL THE WATERS OF THE PLANET EQUAL AND SUITABLE FOR YOUR CONSUMPTION? PROTOCOL OF THE EXPERIMENT: We prepared the area. We Marked 1 Petri Dish Compact Dry® TC (Total counting) and 1 tube of Brilliant Green Bile 2% double concentration broth with the name or number of every sample. We Shaked the sample carefully. With a 10 mL sterile pipette we dropped 10 ml of sample into the tube of Brilliant Green Bile 2% double concentration broth, then we holded pipette at angle so that its loweredge rests against the tube. We incubated the tubes at 37 °C into a yogurt maker container introducing the ubes in a vertical way to avoid any spills. After 1 day we examined the tubes and recorded reactions for gas. We could observe turbidity and gas presence into Durham tubes effervescence when tubes are gently agitated (positive results), Then we Re-incubated gas-negative tubes (without gas) for 1 more day. After that, we could Examine and record reactions. We also Eliminated microorganisms covering it with alcohol before throwing it away.
Total counting of aerobic microorganisms:
We dropped 1 ml of sample with a 1 mL pipette on the middle of the Petri Dish Compact Dry® TC (Total counting). Specimen diffused automatically and evenly into the sheet and transformed the dried sheet into a gel within seconds. We Put the cap again on the plate and turned over the capped plate. After that we let grow in undisturbed warm location, not in sunlight (UV rays are harmful) at room temperature (aprox 22ºC). After 3 days of incubation we counted the number of coloured colonies (it can take more time depending on the environment temperature). After all we eliminated microorganisms covering with alcohol or bleach before throwing it away.
Detection of total coliforms in water
We opened the tub and held the tube on our left hands and used our right little fingers to remove the cap from the tube and flamed the tube mouth. With a 10 mL sterile pipette we dropped 10 ml of sample into the tube of Bril iant Green Bile 2% double concentration broth. We also held pipette at angle so that its lower edge rested against the tube. Then we incubated the tubes at 37°C into a yogurt maker container introducing the tubes in a vertical way to avoid any spil s. After 1 day we examined the tubes and recorded reactions for gas. We could observe turbidity and gas presence into Durham tubes or effervescence when tubes are gently agitated (positive result). We re-incubated gas-negative tubes (without gas) for 1 more day. Then we examined and recorded reactions. We eliminated microorganisms covering with alcohol before we threw it away.