Appendix 10
A serosurvey to trace non-structural proteins to FMDV conducted with the sera from Thrace Region of Turkey Bulut, A.N., Çokçalışkan, C., Alpay, B. FMD Institute (Sap Enstitutusu), PO Box: 714, 06044 Ankara, Turkey
Abstract This paper summarises the results of 3ABC ELISA measuring antibodies to non-structural proteins (NSP’s) of foot-and-mouth disease (FMD) to differentiate infection from vaccination. For this purpose, in total 1310 sera, which were collected from cattle and small ruminants at day 28 and 60 post vaccination were tested by FMD-3ABC ready to use kit, which has been developed by Bommeli Diagnostics. 16 sera were detected as positive in total of those sera, 11 of which are from cattle and 5 from small ruminants. The ratio of positive samples in total was 1.22% (1.6% bovine and 0,7% ovine sera). After this work, a field active surveillance was conducted in epidemiological units where had been collected those positive sera, it was not defined any clinical infection. It was concluded that those animals detected positive relating to NSP antibody might be infected or sub-clinically infected in previous years and that antibody has been still remained as positive. Keywords: 3ABC ELISA, non-structural proteins, FMD 1. Introduction Foot-and-mouth disease (FMD) is highly contagious viral disease, which affects all clovenhoofed animals. It has an economically devastating impact affected countries, since trade barriers, which are imposed where the disease occurs. The detection of antibody to the polyprotein 3ABC of FMDV is the single most reliable indicator of infection (Diago, M de. et. al. 1997) all naive animals seroconvert to this protein following infection. It is known that vaccinated animals, which are exposed to infection, can become persistently infected with FMD without ever showing clinical signs (Mackay, D.K.J., et. al., 1998). Countries, where use vaccination to control FMD outbreaks, it is important to differentiate to antibody against structural protein from non-structural proteins (NSP’s) in order to discriminate virus which is circulated in field either clinical infection or persistence. In the study presented here, sera, which were collected from cattle and small ruminants in Thrace Region of Turkey, were examined by 3 ABC ELISA. 2. Material and Methods 2.1. Test sera Two different group samples were used as test sera: 1st group: in total 1046 sera in 35 villages and 30 large and small ruminants from each village were selected and sera were collected at day 28 post vaccination. 2nd group: 260 sera in 9 villages but different from group first were selected and the blood sera collected at day 60 post vaccination.
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