June 2003 Submit documentation in support of new reference sera to O SKR, A Iran 96 and Asia 1 Shamir to OIE. 2. Phase XVIII 2.1. Background The main aims of this study will be: • • • • •
Introduction of solid phase competition ELISA. Preparation of secondary standards based on reference sera to O, A and Asia 1. An analysis of what exactly is done in this respect by participating laboratories. Use of callibrated tests to examine negative serum populations from own region. Use of callibrated tests to examine a proficiency test panel. Standardisation of internal quality control procedures.
For VNT, participating laboratories should as a minimum, use the homologous virus in their test system. As a working positive control, a titration of a strong positive may be used, so long as the acceptable result is established by comparative tests using the appropriate reference sera. For liquid and solid phase ELISA, O1 Manisa reagents are recommended for serotype O, Asia 1 Shamir for serotype Asia 1 and either A 22 or A Iran 96 for serotype A (latest results need to be analysed before coming to a decision on this). To ensure that the necessary reagents are made available, it will be necessary to send out a plan to participants as soon as possible and to start preparing the documentation (import permits, etc) well in advance of the start date. 2.2. Workplan This will run from January 2003 to December 2004. December 2002 Send consultation letter out on proposals, request plans for preparation of secondary standards and initiate preparation of licences required for reagent transfers, including all possible viruses i.e. O Manisa, O SKR, A22, A Iran 96 and Asia 1 Shamir. Arrange receipt of bulk Asia 1 and A Iran 99 sera from Drs Yadin and Griot. July 2003 Send out reference sera and proficiency panel, along with reagents including detailed protocols for all steps. May 2004 Results to be reported back to WRL for collation and analysis. September 2004 Presentation of results at Research Group meeting.
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