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INNOVATIONSIN

FERMENTATIONAND PHYTOPHARMACEUTICAL TECHNOLOGIES

INNOVATIONSIN FERMENTATIONAND PHYTOPHARMACEUTICAL TECHNOLOGIES

HRUDAYANATHTHATOI

DepartmentofBiotechnology,MaharajaSriramChandra BhanjaDeoUniversity,Baripada,Odisha,India

SONALIMOHAPATRA

DepartmentofBiotechnology,OdishaUniversity ofTechnologyandResearch(FormerlyCollegeof EngineeringandTechnology),Bhubaneswar,Odisha,India

SWAGATKUMARDAS

DepartmentofBiotechnology,OdishaUniversity ofTechnologyandResearch(FormerlyCollegeof EngineeringandTechnology),Bhubaneswar,Odisha,India

Elsevier

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ISBN:978-0-12-821877-8

ForinformationonallElsevierpublications visitourwebsiteat https://www.elsevier.com/books-and-journals

Publisher: SusanDennis

AcquisitionsEditor: AnitaKoch

EditorialProjectManager: EmilyThomson

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CoverDesigner: MarkRogers

TypesetbySTRAIVE,India

PARTAConceptsofreactordesigning

1.Photobioreactorsforproductionofbiodieselfromalgae: Ashortreview3

BikashChandraBehera,HarisankarDey,RiyaJalan, RashmiRanjanMishra,andSonaliMohapatra

1.1 Introduction3

1.2 MethodsofbiodieselproductionfromalgaeusingPBR4

1.3 Conclusion11 References11

2.Mixingandagitationinphotobioreactors13 PauloCesardeSouzaKirnev,LucianaPortodeSouzaVandenberghe, CarlosRicardoSoccol,andJúlioCesardeCarvalho

2.1 Introduction14

2.2 Mixingandagitation Basicconcepts16

2.3 Mechanicalagitationandmixing18

2.4 Pneumaticagitationandmixing23

2.5 Shearsensitivityofmicroalgalcells27

2.6 Conclusion30 References30

3.Membranereactorsformammaliancellculture37

DibyajyotiSamantarayandSwagatKumarDas

3.1 Introduction37

3.2 Membranebioreactortaxonomy38

3.3 Differentialaspectsofmembranebioreactorsoverotherreactors usedincellculture39

3.4 Membranebioreactorsoffervaryingoperationsandmodalities39

3.5 Membranebioreactors:Presentingnumerousopportunitiesin mammaliancellproductmanufacturing42

3.6 Conclusion43 References43

4.Advancesinfermentativesystemsfortheproduction ofethanolfromlignocellulosicbiomass47

M.Láinez,J.A.García-Bejar,G.Flores-Cosío,E.J.Herrera-López, andL.Amaya-Delgado

4.1 Lignocellulosicethanolproductionprocess47

4.2 Fermentationsystems50

4.3 Factorsthataffectalcoholicfermentation60

4.4 Innovativestrategiestoimproveethanolproductivity61

4.5 Modeling,instrumentation,andprocesscontrolstrategies66

4.6 Ethanolproductionatcommercialscale68

4.7 Conclusions70 References70

5.Liquidextractionwithimmobilizedliquidsforproduct recoveryfromfermentationbroths75

AlanD.Perez,AngeloGossi,WolfgangRiedl,BoeloSchuur, andJavierFontalvo

5.1 Introduction75

5.2 Considerationsforliquidextractiontechniquestorecoverproductsfrom fermentationbroths82

5.3 Liquidmembranesinmicrochannels84

5.4 Membrane-assistedextraction93

5.5 Conclusionsandoutlook100 References102

PARTBUpstreamprocessesinbioprocessingoffood, phytopharmaceuticals,andbiofuels

6.Recentadvancementsintheextractionofphytoconstituents fromherbalsources109

DeepakPradhan,PrativaBiswasroy,GoutamRath,andGoutamGhosh

6.1 Introduction109

6.2 Recentadvancesinextractiontechniques113

6.3 Conclusion122 References123

7.Emergingbiosensortechnologyanditspotential applicationinfood127

DeepakKumarVerma,MamtaThakur,SoubhagyaTripathy, BalaramMohapatra,SmitaSingh,AmiR.Patel,AlokKumarGupta, MónicaL.Chávez-González,PremPrakashSrivastav,JoseSandoval-Cortes, andCristóbalN.Aguilar

7.1 Introduction127

7.2 Potentialapplicationinfoodindustry131

7.3 Majorindustrialchallengesandsuggestionsforfoodanalysis140

7.4 Futureoutlooks:Researchdevelopmentandfurther directions144

7.5 Concludingremarks152 References152

8.Currenttrendsingreenprocessing:Improvements offoodproduct165

GauriSinghal,PriyaSingh,AnjaniSihag,andNidhiSrivastava

8.1 Introduction165

8.2 Conceptsandstrategiesofgreenphysicalprocessing166

8.3 Greenphysicaltechniquesandtheirapplicationsinfood improvement167

8.4 Conclusion177 References177

9.Phytochemicalsandtheirnanoformulationinsustained drugdeliveryandtherapy181

UpasanaKapoor-NarulaandNibeditaLenka

9.1 Lifestyle,metabolism,anddiseaselink181

9.2 Conventionaltherapeuticinterventionandlimitations182

9.3 Phytochemicalsasdrugadjuvantsandnaturalsources fortherapeuticintervention182

9.4 Typesofphytochemicalsandtheiruse183

9.5 Bioavailabilityofphytochemicals187

9.6 Targetedtherapy:Theimportanceandrequisites188

9.7 Carriersfordrugtargeting190

9.8 Diseasesandnanodrugdeliveryofphytochemicals202

9.9 SWOTanalysisofphytochemicalconjugatedNPs211

9.10 Conclusion211 References212

10.Biohydrogenevolutioninmicrobialelectrolysiscell, anovelelectrofermentationtechnology: Influenceofreactordesign221

PoojaDange,AnkitKumar,NishitSavla,SantimoyKhilari,SubhasishDutta, PiyushK.Gupta,KrishnaKumarPandey,AbhilashaS.Mathuriya,Kanupriya, SharadAgarwal,andSoumyaPandit

10.1 Introduction221

10.2 Microbialelectrolysiscell229

10.3 ChallengesofEFattheindustriallevel236

10.4 Futureoutlook239

10.5 Conclusion239 References240

PARTCDownstreamprocessesinbioprocessingoffood, phytopharmaceuticals,andbiofuels

11.Mechanism,regulation,andinhibitionofalkaloidsincancer therapytargetingJAK/STATpathway247

SankhadipBose,SabyasachiBanerjee,DeepakKumarVerma,MamtaThakur, SmitaSingh,MónicaL.ChávezGonzález,andCristóbalN.Aguilar

11.1 Introduction247

11.2 StructureofJAKsandSTATs249

11.3 MechanismsofactionofJAK/STATsignalingpathway253

11.4 Roleindevelopmentofcelldivision257

11.5 CoordinationbetweenJAKandSTAT258

11.6 Regulations258

11.7 AlkaloidstargetingJAK/STATpathwayinhibition261

11.8 Concludingremarks267 References267

12.Biologicalproductionofxylitol:Aprocessdevelopment approach271

AshishPrabhu

12.1 Introduction271

12.2 Xylitolanditspotentialapplication273

12.3 Chemicalrouteofxylitolproduction274

12.4 Biologicalformationofxylitol275

12.5 Microorganismusedforxylitolproduction276

12.6 Strainengineeringstrategyforxylitolproduction278

12.7 Factorsaffectingxylitolproduction281

12.8 Bioreactoroperationandcellrecycling283

12.9 Downstreamprocessingofxylitol284 12.10 Conclusion285 Competinginterests286 References286

13.Productionofmethylethylketoneapplyingprocess intensificationstrategies295

EduardoSánchez-Ramírez,GabrielContreras-Zarazua, AnaGabrielaRomero-García,BrendaHuerta-Rosas, JuanJoseQuiroz-Ramirez,andJuanGabrielSegovia-Hernández

13.1 Introduction296

13.2 Casesofstudy300

13.3 Methodologytodesigntheintensifiedalternatives301

13.4 Performanceevaluationindices304

13.5 Results306

13.6 Conclusions311 References312

14.Microbialxylanases,theirstructuralcharacteristics,and industrialapplications:Abiotechnologicaladvancement315 ManishPaulandHrudayanathThatoi

14.1 Introduction315

14.2 Lignocellulolyticenzymes316

14.3 Lignocellulolyticmicroorganisms317

14.4 Xylanase322

14.5 Applicationofxylanase326

14.6 Isolationandscreeningofxylanolyticbacteria327

14.7 Growthprofileanalysis329

14.8 Biochemicalidentification329

14.9 Enzymeassayfordeterminationofxylanaseactivity330

14.10 Optimizationstrategyforproductionofbacterialxylanase enzyme330

14.11 Insilicostudy331

14.12 Conclusion333 References334

15.Advancesinfermentedfoodsandtherapeutics341

PankajTaneja,SamuelShiferawBiresaw,NeetuKumraTaneja, SaurabhKumarJha,BelayZeleke,SaumyaSrivastava,MayankTaneja, andSahdeoPrasad

15.1 Introduction341

Contents

15.2 Humanevolutionandnutrition342

15.3 Fermentedfoodsonsocietyperspective343

15.4 Healthbenefitsoffermentedfoodsandbeverages345

15.5 Potentialbenefitsandrisksoffermentation346

15.6 Theroleofgutmicrobiota347

15.7 Gutmicrobiota,fermentedfoods,andbeverages349

15.8 Conclusions352 Acknowledgments352 Conflictofintereststatement352 References352

PARTDBy-productutilizationforproduction ofvalue-addedproducts

16.Winewasteasapotentialsourceofbioactivecompounds361

DaisyAmaya-Chantaca,AdrianaC.Flores-Gallegos,AnnaIliná, CristóbalN.Aguilar,DeepakKumarVerma,DeepikaBaranwal, andMónicaL.Chávez-González

16.1 Introduction361

16.2 Grapecomposition362

16.3 Grapewaste365

16.4 Commonusesofgrapewaste367

16.5 Bioactivecompoundsinwinewaste367

16.6 Solidandsubmergedfermentation372

16.7 Concludingremarks375 Acknowledgments375 References375

17.Agro-industrialwastesforproductionofsingle-cell protein(SCP)381

RocíodelCarmenCarranza-Mendez,LeonardoSepúlveda-Torre, RodolfoRamos-González,DeepakKumarVerma,DeepikaBaranwal, CristóbalN.Aguilar,andMónicaL.Chávez-González

17.1 Introduction381

17.2 Single-cellprotein382

17.3 SCPproductionfromagro-industrialwastes386

17.4 FactorsaffectingtheSCPprocess390

17.5 AdvantagesanddisadvantagesofSCP393

17.6 Concludingremarks393 Acknowledgments393 References394

18. β-Glucanproductionthroughbioconversionofsugarcane bagasseby Saccharomycescerevisiae and Aspergillusniger 397

GemilangLaraUtama,MegaOktavianiKurniawan,YanaCahyana, andRoostitaLoboBalia

18.1 Introduction398

18.2 Materialsandmethods399

18.3 Resultsanddiscussion401

18.4 Conclusion411

Acknowledgments411

Ethicsapprovalandconsenttoparticipate412

Consentforpublication412 Availabilityofdataandmaterials412

Competinginterests412

Funding412

Authors’ contributions412 References412

19.Value-addedproductdevelopmentfromfoodscraps417 VartikaVerma,LavishaRao,SunandaJoshi,MonikaChoudhary, andNidhiSrivastava

19.1 Introduction417

19.2 Foodwasteasarenewablesourcedmaterial419

19.3 Applicationofwastematerialsinthedevelopmentofvalue-added productlines419

19.4 Someoftheothervalue-addedproductsfromfoodwaste426

19.5 Conclusions429 References430 Furtherreading435

20.Evaluationofvariouspossibilitiesofunderutilizedfish processingbiomassasavalue-addedproduct: Awaste-to-wealthapproach437 SupriyaDash,ShyamaliniShyamasuta,andPratikDash

20.1 Introduction437

20.2 Fishprocessingbiomass438

20.3 Bioactivecompoundspresentinfishbiomassandtheirutilization441

20.4 Bioactivecompoundsfromfish445

20.5 Conclusion452

References453 Furtherreading455

21.Value-addedproductsfromindustrialwastesof phytopharmaceuticalindustries457

MdNurKabidulAzam,TusharAhmedShishir,AmiaKhandker, andMdNazmulHasan

21.1 Introduction457

21.2 Drugandmedicineinmodernphytopharmaceutical approaches461

21.3 Phytopharmaceuticalwastes462

21.4 Value-addedproductsfromphytopharmaceuticals463

21.5 Biomass465

21.6 Bioethanol469

21.7 Enzymeproduction470

21.8 Composting474

21.9 Organicfertilizer475

21.10 Fishandpoultryfeed476

21.11 Microbialconversion476

21.12 Conclusion480 References480 Index 491

Contributors

SharadAgarwal

SchoolofBasicSciencesandResearch,ShardaUniversity,GreaterNoida,UttarPradesh, India

Cristo ´ balN.Aguilar

BioprocessesandBioproductsResearchGroup,FoodResearchDepartment,Schoolof Chemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo,Coahuila,Mexico

DaisyAmaya-Chantaca

BioprocessesandBioproductsResearchGroup,FoodResearchDepartment;NanobioscienceGroup,SchoolofChemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo, Coahuila,Mexico

L.Amaya-Delgado

IndustrialBiotechnologyUnit,CenterforResearchandAssistanceinTechnologyand DesignoftheStateofJalisco,A.C.,Zapopan,Jalisco,Mexico

MdNurKabidulAzam

LaboratoryofPharmaceuticalBiotechnology,DepartmentofGeneticEngineeringand Biotechnology,JashoreUniversityofScienceandTechnology,Jashore;Departmentof Biotechnology,TechBNutrigenomics,Dhaka,Bangladesh

RoostitaLoboBalia

VeterinaryStudyProgram,FacultyofMedicine,UniversitasPadjadjaran,Bandung;Faculty ofAnimalHusbandry,UniversitasPadjadjaran,Sumedang,Indonesia

SabyasachiBanerjee

GuptaCollegeofTechnologicalSciences,Asansol,WestBengal,India

DeepikaBaranwal

DepartmentofHomeScience,AryaMahilaPostGraduateCollege,Varanasi,UttarPradesh, India

BikashChandraBehera

SchoolofBiologicalSciences,NISER,Bhubaneswar,Odisha,India

SamuelShiferawBiresaw

DepartmentofBiotechnology,ShardaUniversity,GreaterNoida,UttarPradesh,India

PrativaBiswasroy

SchoolofPharmaceuticalSciences,Siksha‘O’Anusandhan(DeemedtobeUniversity), Bhuabaneswar,Odisha,India

SankhadipBose

BengalSchoolofTechnology,ACollegeofPharmacy,Hooghly,WestBengal,India

YanaCahyana

FacultyofAgro-IndustrialTechnology,UniversitasPadjadjaran,Sumedang,Indonesia

Mo ´ nicaL.Cha ´ vez-Gonza ´ lez

NanobioscienceGroup;BioprocessesandBioproductsResearchGroup,FoodResearch Department,SchoolofChemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo,Coahuila, Mexico

MonikaChoudhary

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Banasthali,Rajasthan, India

GabrielContreras-Zarazua

DepartamentodeIngenierı´aQuı´mica,UniversidaddeGuanajuato,Guanajuato,Mexico

PoojaDange

AmityInstituteofBiotechnology,AmityUniversity,Mumbai,Maharashtra,India

SwagatKumarDas

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

PratikDash

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

SupriyaDash

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

Ju ´ lioCesardeCarvalho

BioprocessEngineeringandBiotechnologyDepartment,FederalUniversityofParana ´ ,ACF CentroPolitecnico,Curitiba,Parana ´ ,Brazil

PauloCesardeSouzaKirnev

BioprocessEngineeringandBiotechnologyDepartment,FederalUniversityofParana ´ ,ACF CentroPolitecnico,Curitiba,Parana ´ ,Brazil

LucianaPortodeSouzaVandenberghe

BioprocessEngineeringandBiotechnologyDepartment,FederalUniversityofParana ´ ,ACF CentroPolitecnico,Curitiba,Parana ´ ,Brazil

Rocı´odelCarmenCarranza-Mendez

BioprocessesandBioproductsResearchGroup,FoodResearchDepartment; NanobioscienceGroup,SchoolofChemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo, Coahuila,Mexico

HarisankarDey

P.G.DepartmentofBotany,RamakrishnaMissionVivekanandaCentenaryCollege, Kolkata,WestBengal,India

SubhasishDutta

ChemicalEngineeringDivision,CenterofInnovativeandAppliedBioprocessing(CIAB), Mohali,Punjab,India

G.Flores-Cosı ´ o

IndustrialBiotechnologyUnit,CenterforResearchandAssistanceinTechnologyand DesignoftheStateofJalisco,A.C.,Zapopan,Jalisco,Mexico

AdrianaC.Flores-Gallegos

BioprocessesandBioproductsResearchGroup,FoodResearchDepartment,Schoolof Chemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo,Coahuila,Mexico

JavierFontalvo

LaboratoryofProcessIntensificationandHybridSystems,FacultyofEngineeringand Architecture,UniversidadNacionaldeColombia,CampusLaNubia,Manizales,Colombia

J.A.Garcı´a-Bejar

IndustrialBiotechnologyUnit,CenterforResearchandAssistanceinTechnologyand DesignoftheStateofJalisco,A.C.,Zapopan,Jalisco,Mexico

GoutamGhosh

SchoolofPharmaceuticalSciences,Siksha‘O’Anusandhan(DeemedtobeUniversity), Bhuabaneswar,Odisha,India

AngeloGossi

UniversityofTwente,FacultyofScienceandTechnology,SustainableProcessTechnology Group,Enschede,TheNetherlands;UniversityofAppliedSciencesandArtsNorthwestern Switzerland,SchoolofLifeSciences,InstituteforChemistryandBioanalytics,Muttenz, Switzerland

AlokKumarGupta

DivisionofPostharvestManagement,ICAR-CentralInstituteforSubtropicalHorticulture (MinistryofAgricultureandFarmersWelfare,GovernmentofIndia),Lucknow,Uttar Pradesh,India

PiyushK.Gupta

SchoolofBasicSciencesandResearch,ShardaUniversity,GreaterNoida,UttarPradesh, India

MdNazmulHasan

LaboratoryofPharmaceuticalBiotechnology,DepartmentofGeneticEngineeringand Biotechnology,JashoreUniversityofScienceandTechnology,Jashore,Bangladesh

E.J.Herrera-Lo ´ pez

IndustrialBiotechnologyUnit,CenterforResearchandAssistanceinTechnologyand DesignoftheStateofJalisco,A.C.,Zapopan,Jalisco,Mexico

BrendaHuerta-Rosas

DepartamentodeIngenierı´aQuı´mica,UniversidaddeGuanajuato,Guanajuato,Mexico

AnnaIlina ´

NanobioscienceGroup,SchoolofChemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo, Coahuila,Mexico

RiyaJalan

DepartmentofBiotechnology,MITSSchoolofBiotechnology,Bhubaneswar,Odisha,India

SaurabhKumarJha

DepartmentofBiotechnology,ShardaUniversity,GreaterNoida,UttarPradesh,India

SunandaJoshi

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Banasthali,Rajasthan, India

Kanupriya

SchoolofBasicSciencesandResearch,ShardaUniversity,GreaterNoida,UttarPradesh, India

UpasanaKapoor-Narula

NationalCentreforCellScience,Pune,India

AmiaKhandker

DepartmentofBiotechnology,TechBNutrigenomics,Dhaka,Bangladesh

SantimoyKhilari

DepartmentofChemistry,GuruGhasidasVishwavidyalaya,Bilaspur,Chhattisgarh,India

AnkitKumar

SchoolofBasicSciencesandResearch,ShardaUniversity,GreaterNoida,UttarPradesh, India

NeetuKumraTaneja

DepartmentofMicrobiology,NationalInstituteofFoodTechnologyandEntrepreneurship andManagement,Sonipat,Haryana,India

MegaOktavianiKurniawan

FacultyofAgro-IndustrialTechnology,UniversitasPadjadjaran,Sumedang,Indonesia

M.La ´ inez

IndustrialBiotechnologyUnit,CenterforResearchandAssistanceinTechnologyand DesignoftheStateofJalisco,A.C.,Zapopan,Jalisco,Mexico

NibeditaLenka

NationalCentreforCellScience,Pune,India

AbhilashaS.Mathuriya

CRZandIndustry-III,EnvironmentalImpactAssessmentDivision,MinistryofEnvironment,ForestandClimateChange,GovernmentofIndia,NewDelhi,India

RashmiRanjanMishra

DepartmentofBiotechnology,MITSSchoolofBiotechnology,Bhubaneswar,Odisha,India

BalaramMohapatra

DepartmentofBioscienceandBioengineering,IndianInstituteofTechnologyBombay, Bombay,Maharashtra,India

SonaliMohapatra

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

KrishnaKumarPandey

DepartmentofPhysics,SchoolofBasicSciencesandResearch,ShardaUniversity,Greater Noida,UttarPradesh,India

SoumyaPandit

SchoolofBasicSciencesandResearch,ShardaUniversity,GreaterNoida,UttarPradesh, India

AmiR.Patel

DivisionofDairyMicrobiology,MansinhbhaiInstituteofDairy&FoodTechnologyMIDFT,Mehsana,Gujarat,India

ManishPaul

DepartmentofBiotechnology,MaharajaSriramChandraBhanjaDeoUniversity,Baripada, Odisha,India

AlanD.Perez

UniversityofTwente,FacultyofScienceandTechnology,SustainableProcessTechnology Group,Enschede,TheNetherlands;LaboratoryofProcessIntensificationandHybridSystems,FacultyofEngineeringandArchitecture,UniversidadNacionaldeColombia,Campus LaNubia,Manizales,Colombia;UniversityofAppliedSciencesandArtsNorthwestern Switzerland,SchoolofLifeSciences,InstituteforChemistryandBioanalytics,Muttenz, Switzerland

AshishPrabhu

DepartmentofBiotechnology,NationalInstituteofTechnologyWarangal,Warangal, Telangana,India

DeepakPradhan

SchoolofPharmaceuticalSciences,Siksha‘O’Anusandhan(DeemedtobeUniversity), Bhuabaneswar,Odisha,India

SahdeoPrasad

DepartmentofImmunotherapeuticsandBiotechnology,TexasTechUniversityofHealth ScienceCenter,Abilene,TX,UnitedStates

JuanJoseQuiroz-Ramirez

CONACyT-CIATECA.CCentrodeInnovacio ´ nAplicadaenTecnologı´asCompetitivas, Leo ´ n,Mexico

RodolfoRamos-Gonza ´ lez

NanobioscienceGroup,SchoolofChemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo, Coahuila,Mexico

LavishaRao

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Banasthali,Rajasthan, India

GoutamRath

SchoolofPharmaceuticalSciences,Siksha‘O’Anusandhan(DeemedtobeUniversity), Bhuabaneswar,Odisha,India

WolfgangRiedl

UniversityofAppliedSciencesandArtsNorthwesternSwitzerland,SchoolofLifeSciences, InstituteforChemistryandBioanalytics,Muttenz,Switzerland

AnaGabrielaRomero-Garcı ´ a

DepartamentodeIngenierı´aQuı´mica,UniversidaddeGuanajuato,Guanajuato,Mexico

DibyajyotiSamantaray

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

EduardoSa ´ nchez-Ramı ´ rez

DepartamentodeIngenierı´aQuı´mica,UniversidaddeGuanajuato,Guanajuato,Mexico

JoseSandoval-Cortes

FoodResearchDepartment,SchoolofChemistry,AutonomousUniversityofCoahuila, Saltillo,Mexico

NishitSavla

AmityInstituteofBiotechnology,AmityUniversity,Mumbai,Maharashtra,India

BoeloSchuur

UniversityofTwente,FacultyofScienceandTechnology,SustainableProcessTechnology Group,Enschede,TheNetherlands

JuanGabrielSegovia-Herna ´ ndez

DepartamentodeIngenierı´aQuı´mica,UniversidaddeGuanajuato,Guanajuato,Mexico

LeonardoSepu ´ lveda-Torre

BioprocessesandBioproductsResearchGroup,FoodResearchDepartment,Schoolof Chemistry,UniversidadAuto ´ nomadeCoahuila,Saltillo,Coahuila,Mexico

TusharAhmedShishir

DepartmentofMathematicsandNaturalSciences,BRACUniversity,Dhaka,Bangladesh

ShyamaliniShyamasuta

DepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(Formerly CollegeofEngineeringandTechnology),Bhubaneswar,Odisha,India

AnjaniSihag

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Tonk,Rajasthan,India

PriyaSingh

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Tonk,Rajasthan,India

SmitaSingh

DepartmentofLifeSciences(FoodTechnology),GraphicEra(Deemedtobe)University, Dehradun,Uttarakhand,India

GauriSinghal

DepartmentofBiotechnology,SchoolofBasicandAppliedSciences,SanskritiUniversity, Mathura,UttarPradesh,India

CarlosRicardoSoccol

BioprocessEngineeringandBiotechnologyDepartment,FederalUniversityofParana ´ ,ACF CentroPolitecnico,Curitiba,Parana ´ ,Brazil

PremPrakashSrivastav

AgriculturalandFoodEngineeringDepartment,IndianInstituteofTechnologyKharagpur, Kharagpur,WestBengal,India

NidhiSrivastava

DepartmentofBiotechnology,NationalInstituteofPharmaceuticalEducationandResearch (NIPER),Lucknow,UttarPradesh,India

SaumyaSrivastava

DepartmentofBiotechnology,ShardaUniversity,GreaterNoida,UttarPradesh,India

MayankTaneja

NetajiSubhashInstituteofTechnology,Dwarka,Delhi,India

PankajTaneja

DepartmentofBiotechnology,ShardaUniversity,GreaterNoida,UttarPradesh,India

MamtaThakur

DepartmentofFoodEngineeringandTechnology,SantLongowalInstituteofEngineering andTechnology,Longowal,Punjab,India

HrudayanathThatoi

DepartmentofBiotechnology,MaharajaSriramChandraBhanjaDeoUniversity,Baripada, Odisha,India

SoubhagyaTripathy

AgriculturalandFoodEngineeringDepartment,IndianInstituteofTechnologyKharagpur, Kharagpur,WestBengal,India

GemilangLaraUtama

FacultyofAgro-IndustrialTechnology,UniversitasPadjadjaran,Sumedang;Centrefor EnvironmentandSustainabilityScience,UniversitasPadjadjaran,Bandung,Indonesia

DeepakKumarVerma

AgriculturalandFoodEngineeringDepartment,IndianInstituteofTechnologyKharagpur, Kharagpur,WestBengal,India

VartikaVerma

DepartmentofBioscienceandBiotechnology,BanasthaliVidyapith,Banasthali,Rajasthan, India

BelayZeleke

DepartmentofBiotechnology,ShardaUniversity,GreaterNoida,UttarPradesh,India

PARTA

Conceptsofreactor designing

Photobioreactorsforproduction ofbiodieselfromalgae:Ashort review

BikashChandraBeheraa,HarisankarDeyb,RiyaJalanc, RashmiRanjanMishrac,andSonaliMohapatrad

aSchoolofBiologicalSciences,NISER,Bhubaneswar,Odisha,India bP.G.DepartmentofBotany,RamakrishnaMissionVivekanandaCentenaryCollege,Kolkata, WestBengal,India cDepartmentofBiotechnology,MITSSchoolofBiotechnology,Bhubaneswar,Odisha,India dDepartmentofBiotechnology,OdishaUniversityofTechnologyandResearch(FormerlyCollegeof EngineeringandTechnology),Bhubaneswar,Odisha,India

1.1Introduction

Algaeuselightandcarbondioxidetogrowinaquaticenvironments.Thereare twotypesofalgae:macro-andmicroalgae.Duetoexcessiveoilcontentand higherbiomassproductioncapabilityinvariousenvironments,microalgae areconsideredanassetforbiofuelproduction.Inrecentyears,microalgaeas analternativebiofuelfeedstockhasattractedtheinterestofresearchers,entrepreneurs,andthegeneralpublic(ElMekawyetal.,2014).Algalbiomasscontainsthreemaincomponents:carbohydrates,proteins,andlipids/naturaloils (Fig.1.1).Thenaturaloilmadebymicroalgaeisintheformoftricylglycerol. Microalgaearethereforetheexclusivefocusinthealgae-to-biodieselarena.

Thelarge-scaleproductionofmicroalgalbiomassisgenerallycarriedout throughcheapandsimpleopenpondtechnologies.However,thereare somedisadvantageswiththismethodsuchaschanceofcontamination, waterevaporation,lowgas-liquidmasstransferrate,lowmixingrate,and poortemperaturecontrol(Chisti,2007).Toovercomesuchdisadvantages, numerousclosedphotobioreactors(PBR)haverecentlybeendesigned. Thistechnologyisverycost-effectiveforalgalbiomassproduction.Itprovidesacloseenvironmentthatcanprotecttheculturefromcontamination. ThroughPBRtechnologies,differentfavorablegrowthconditionssuchas pH,temperature,O2,etc.,requiredformicroalgalgrowthcanbecontrolled moreaccurately(Tredicietal.,2010).

Fig.1.1 Differentmetabolicpathwaysinmicroalgaeforthesynthesisofproteins, nucleicacids,carbohydrates,lipids,andH2 thatarerelatedtobiofuelproduction (Beeretal.,2009).

Hence,thisreviewaimstoelucidatetheuseofclosedPBRsforbiodiesel productionusingmicroalgaebiomass.Factorsaffectingbiodieselproduction suchasdifferentlipidextractionmethodsandbiodieseldetectiontechniques arealsodescribedindetail.

1.2MethodsofbiodieselproductionfromalgaeusingPBR

1.2.1Tubularphotobioreactors

Tubularphotobioreactors(TPBRs)aremadefromglassorplastictubes orientedhorizontallyorvertically(Fig.1.2).TPBRsaresuppliedfroma centralutilityinstallationwithsensors,pumps,respectivenutrients,and CO2 supply.InahorizontalTPBR,thetubesarearrangedinmultipleorientationssuchashorizontal,inclined,spiral,helicoidal,andtheirvariations. BothtypesofTPBR(horizontalandvertical)differaccordingtothelength ofthetube,flowvelocity,rateofcirculationinthesystem,configurationof thelightreceiver,etc.InaTPBR,thetubesusedhavediametersbetween

Fig.1.2 (A)Designofatubularphotobioreactor(Tanwaretal.,2010).(B)Threedimensionaldiagramofatubularphotobioreactor(Bahadar,2014).

10and60mm,withlengthsaccordingtotheheightoftheTPBRdesigned. Theuseofsuchtubeshelpsinachievingahighsurface-to-volumeratio (above100/m),whichisoneofthemainadvantagesofthisdesign (Posten,2009).However,duetohugeenergyrequirementsandthegreater costofinvestment(particularlyformixingandcooling),theiruseisveryrare inindustries.

1.2.1.1Stirredtankphotobioreactors

Instirredtankphotobioreactors(STPBRs),agitationiscarriedoutmechanicallywiththehelpofimpellersofdifferentsizesandshapes.Several worksareperformedthroughthesesuchasaeration,propermixingand

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