Application of Piezoelectric Technology in Laser Confocal Scanning Microscope
Application of Piezoelectric Technology in Laser Confocal Scanning Microscope Laser confocal scanning microscope is a technology developed and widely used in the past ten years. It uses a laser as a light source and consists of a confocal imaging scanning system, an electron optical system and an image analysis system. After the beam is focused, it falls on tiny points at different depths of the sample (tissue slab or cell), and moves and scans, so that the image formed by the reflected light at any point in the sample can be accurately received and a signal is generated, and then connected The image analysis system performs analysis processing. It is an advanced cell and molecular biological analysis instrument, which is more and more widely used in biology and medicine, and has become an essential tool for biomedical experimental research.
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Traditional fluorescence microscopes use fluorescent substances to mark specific structures in cells, the contrast between the image and the background is enhanced, and the light source uses short-wavelength ultraviolet light, which greatly improves the resolution. However, when the observed fluorescent specimen is slightly thicker, an insurmountable disadvantage of traditional fluorescence microscopes is manifested: the fluorescent structures outside the focal plane are blurred and blurred. The reason is that most biological specimens are overlapping structures with different layers, and the changes in the focal plane under ordinary light microscopes will show different morphologies. If the fluorescently labeled structures are distributed at different levels and overlap each other, reflected fluorescence microscopy not only collects light from the focal plane, but also the scattered fluorescence from above or below the focal plane is also received by