Chromatography Workflow

Knowing the Chromatography Workflow Consisting of many different and evolving techniques, chromatography is used across multiple branches of science to separate molecules. With a focus on preparation and/or analysis of these molecules, there are two main phases of chromatography used for separating components: the mobile phase and the stationary phase. For glassware, labware and life science benchtop equipment suggestions by chromatography phase, please see the following pages.

Preparation

When preparing the column, careful thought must be given to provide best sample separation. Consider sample polarity, size, and affinity. Preparation is dictated by what type of chromatography is used – analytical or preparative.

Running the Column

Columns must be set up properly for samples to run effectively. Factors like loading the column or making sure it is vertically straight, could make a vast difference in sample collection.

Fraction Collection

Fraction collection varies, depending on how well you can visualize your sample on the column. Best practice dictates to collect many small fractions which can be pooled together once their purity is confirmed.

Analysis

Thin layer chromatography is a common technique to confirm which fraction contains the desired eluent. Additional techniques like UV/Vis spectrophotometry can be used separately or in concert.

Concentration

Often, the pure sample must be at a higher concentration than what the pooled fractions permit. Tools such as evaporators are available.

Clean Up

Properly cleaned glassware and plasticware allows for confidence in future analyses using the same methodology.

Glassware, Labware, & Benchtop Equipment for

The Chromatography Workf low SP chromatography labware, glassware and life science benchtop equipment are developed with the scientist in mind, helping to find a more efficient solution for individual workflows. The comprehensive and cross-discipline tools available are outlined on the following pages.

Preparation Mobile phase must be mixed and stored properly. Our products offer novel solutions to keep your mobile phase mixed and contained.

Running the Column

Fraction Collection

When setting up your SP Wilmad-LabGlass chromatography columns, tools are needed to ensure your mobile phase is added gently to the column for the tightest sample band.

SP Bel-Art labware carries various rack sizes for any scale of collection – from microcentrifuge tube to beaker.

Analysis

Concentration

Clean Up

SP Wilmad-LabGlass has chambers and reagent atomizers that you need to visualize your eluent.

Using the most advanced proven technology in evaporation science, SP Genevac evaporators are designed for solvent removal for the concentration of samples.

Test tubes can be disposed of after use, but can also easily be washed using SP Bel-Art test tube baskets or SP Hotpack glassware washers.

Running the Column

Preparation

Fraction Collection

HPLC Reservoir Secondary Containers

Chromatography Column Holder

Polypropylene Graduated Griffin Low Form Beakers

Stainless Steel Micro Spoon and Spatula Weighing Set

Lattice Support Stand

Polypropylene Conical Tube Holder; For 15ml and 50ml Tubes

Pickup Adapter w/Valve

Economy Support Stand

Microcentrifuge Tube Ice Rack/Tray

MP Cap 3-Hole Economy

Heavy Duty Support Stand

Polypropylene Wire Rack I.D. Tags

Red GL45 Cap w/PTFE Liner

Polypropylene Compact Support Stand

Write-On Label Tape Multi-Pack

MP Cap 3-Hole GL45

Stainless Steel Bosshead

Poxygrid Test Tube Racks

Glass Frit

Spring Action 9 in. Burette Clamp Clear View Polycarbonate Fume Hoods

Indexed Test Tube Racks

Fabricated Polypropylene Fume Hood

Reversible PCR and Microcentrifuge Tube Racks

Flash Chromatography Reservoir

Microcentrifuge Tube Racks

Flow Controller

Low Form Beaker

MP Cap Filter/Degassing

Polypropylene Drum and Carboy Funnels Polypropylene Micro Funnels Polypropylene Standard Stem Funnels Poxygrid Pasteur Pipette Can Rack Roto-Flo Polystyrene Flow Indicators Clear Polycarbonate Flow Indicator Vikem Vinyl Pipette Filler Flash Chromatography Column w/PTFE Plug Straight Chromatography Columns w/PTFE Plug Straight Chromatography Columns w/PTFE Plug & Fritted Disc Straight Chromatography Columns w/PTFE Plug & Fritted Disc

No-Wire Test Tube Grip Racks

Analysis

Concentration

Clean Up

Complete TLC Developing Tanks

EZ-2 4.0 Benchtop Evaporator

Polyethylene Safety Waste 20 Liter (5 Gallon) Jug

TLC Developing Tanks w/Screw Cap

HT Series 3i High Throughput Evaporator

GHS Labeled Right-to-Know Safety Vented Wash Bottles

TLC Sprayers

Round Bottom Flasks 1N

Polypropylene Wash Bottle Carrier

Clear Mini-Vials w/Screw Cap

Polyethylene Two Bottle Carrier

Standard RotoVap Evaporator Advanced RotoVap Evaporator

Polypropylene Test Tube Baskets & Lids Vakuwash Cuvette Washer Polypropylene Spill Containment Tray Glassware Washers

To contact our dedicated SP brand specialists, see below:

© 2022 Bel-Art Products, a part of SP. All rights reserved. SP Bel-Art, SP Hotpack, SP Genevac and SP Wilmad-LabGlass are brands of SP (scientificproducts.com), provider of biopharma processing equipment, life sciences equipment, scientific glassware and laboratory supplies used in the pharmaceutical, biotechnology, academic, food and beverage and industrial markets. All trademarks referenced in this document are registered or common law trademarks of SP Industries, Inc. or its affiliates in the U.S. and/or other countries.

Contact SP Bel-Art:

Contact SP Hotpack:

Contact SP Genevac:

Contact SP Wilmad-LabGlass:

Bel-Art Products, a part of SP 661 Route 23 South Wayne, New Jersey 07470 USA U.S.: +1-800-423-5278 Intl: +1-973-694-0500 cservice.belart@spindustries.com

SP Corporate Office 935 Mearns Road Warminster, PA 18974 USA U.S.: +1-800-523-2327 Intl: +1-215-672-7800 sales@spscientific.com

Genevac Ltd. Farthing Road, Ipswich Suffolk, IP1 5AP U.K.: +44 (0)1473 240000 Intl: +845-255-5000 sales@genevacusa.com

SP Wilmad-LabGlass, an SP brand 1172 NW Boulevard, Vineland, NJ 08360 USA U.S.: +1-800-220-5171 Intl: +1-856-691-3200 cs@wilmad-labglass.com