SWQC Dudley Pond 2010 Year-end Report DEP322-698 BINDER

Surface Water Quality Committee

Brian Monahan Wayland Conservation Commission Town Building 41 Cochituate Road Wayland, MA 01778

December 17, 2010

Subject: Dudley Pond Order of Conditions (DEP 322-698) – 2010 End of Season Report Conditions:

MGL 131 §40 Wayland Ch 194

#33 Post treatment season report. #13 Post treatment season report

Commissioners: Attached is the SWQC’s 2010 year-end report for Dudley Pond. This season handpulling and spot herbicide treatment with triclopyr (Renovate OTF) were utilized.

A PDF detailed work log accompanies the emailed PDF of the report.

We would like a 15 minute time slot on your calendar to recap and discuss the report with the Conservation Commission. As of today, we do not expect that in 2011 we will need to spot treat with herbicide.

Best regards,

Michael P. Lowery, Applicant Wayland Surface Water Quality Committee

DEP 332-693 Dudley Pond

2010 End of Season Report to the Wayland Conservation Commission

Wayland Surface Water Quality Committee

Dudley Pond – DEP 332-698 2010 End of Season Report

SUMMARY The 2010 hand-pulling and spot treatment program was highly successful. No milfoil was detected at end-of-season.

Accomplishments: Hand-pulling: Kept Eurasian Water Milfoil under control thru 287 diver/snorkeler hours of hand-pulling, removing 6145 plants by the roots.

Spot-treatment: Renovate OTF (triclopyr) was applied to 18.5 acres Funding: Continued the principal of 25% shared funding with the DPA whose members care deeply about Dudley Pond and who directly enjoy its benefits.

The DPA provided extra funds to complete spot treatment when SWQC budget was exhausted.

Minimized chemical treatment through continued aggressive hand-pulling.

Continued consensus for limiting herbicide use with goals and programs to minimize its use.

Completed annual testing for detection of herbicide in Happy Hollow wells.

Completed a comprehensive 9.75 hour under-water survey at season-end.

Yearend status: No milfoil found by either a rake-toss survey nor in underwater inspections.

Observations: Both before and after spot treatment, milfoil plants were farther dispersed, and pulling conditions became less clear - making divers less productive: $1.87/plant vs. $0.79/plant in 2009.

Triclopyr effects were seen throughout the pond.

Related Activities: Dudley Pond circulators were moved to the Chateau area to see if they might be more successful operating in shallower areas – no such effect was apparent.

Water clarity remained significantly better that expected during the year, although 6-8 weeks after spot treatment, clarity diminished making it difficult to continue hand-pulling.

WSWQC is developing proposed septic and landscaping regulations which would require additional measures for areas in close proximity to water bodies.

2011 Dudley Pond Plans: SWQC has budged for, and plans to continue aggressive hand-pulling at the same level or higher than 2010 – and we are looking for means to extend our pulling season and make divers more productive. Herbicide use is not planned.

SWQC plans to begin a multi-year, systematic sampling program for phosphorus, chlorophyll-a together with temperature, Secchi-depth, and dissolved oxygen.

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2009 HISTORY – Hand Pulling Late Season Growth – Spot treatment would be required in 2010 Significant late-season new growth appeared on seemingly dead stems. We contacted Aquatic Control Technologies, and arranged a visit by Marc Bellaud, aquatic biologist.

We noticed this in 6-8’ deep areas in the middle of the pond, which we came to later in the season. Bright, lime green growth emerged from brown, bare stems.

Bellaud indicated that that this was not uncommon in other lakes and after treatment with other herbicides. He suggested that we prepare a contingency plan for spot treating the areas.

The end-of-season survey located hundreds of plant clusters. The survey suggests both where we should start in 2010 and where spot treatments might be needed.

Figure 1 - 2009 End of season milfoil clusters (color indicates only survey date)

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 HAND-PULLING Milfoil was more scattered than in prior years. Instead of marking individual plants, areas to be scanned were marked, sometimes by divers themselves after underwater searches.

Divers would update a detailed web form, and were paid based on their posted updates. Notes were kept on ‘seeing’ conditions, water temperature, and comments on plants. (See accompanying PDF) Ted Fiust and Allison LaClaire were our principal divers. Both are familiar with and lived on Dudley Pond during the 2010 season. Three other experienced divers were provided by Aquatic Vision LLC (Ted Fiust’ firm).

2010 hand-pulling began April 26th, and continued through June 15th. By mid-June visibility decreased diver effectiveness – but diving work ended primarily because there were few plants remaining to pull.

Figure 2 - Allison LaClaire & Ted Fiust

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 HAND-PULLING RECAP 6145 plants were removed by the roots. Two principal divers and three other divers and snorkelers logged 287 hours under water.

To water users on the surface, there were no impediments to use – and no apparent milfoil.

All harvesting was recorded using the area maps first created in 2008.

Area 1A 1E 1F 2A 2B 2D 2E 2F 2G 3B 3C 4E Grand Total

PLANTS 1,212 316 54 290 1,912 271 6 28 57 702 767 530

HOURS 102.00 6.10 1.50 20.00 66.00 18.50 1.00 2.00 1.80 15.00 30.80 32.00

6,145

296.70

Cost per plant: 6,145 plants were hand pulled in 2010, compared to 18,237 in 2009 2010

PLANTS 6,145

2009

PLANTS 18,237

287.2 $11,488.00 $1.87

hours cost per plant

358 $ 14,320.00 $0.79

hours

Wayland Surface Water Quality Committee

cost per plant

5

Dudley Pond – DEP 332-698 2010 End of Season Report

2010 END OF SEASON SURVEY Our active management season ended early – because we were unable to find any plants to pull.

Under water diver survey During September 13,14 and 21,22 diver Allison LaClaire spent 9.5 under-water hours searching patterns in areas where we expected to find milfoil plants. No milfoil plants were found:

Figure 3 - 2010 End of season - diver survey

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 END OF SEASON SURVEY - continued Rake-toss survey On September 24th, Marc Bellaud, ACT Aquatic Biologist and Mike Lowery of WSWQC did a visual inspection of Dudley Pond along the track shown below. At the green starred points below, Bellaud did a rake toss to pull up plants from the pond bottom. No milfoil plants were found.

Figure 4 - 2009 End of season ACT rake-toss survey

Since 1991 when milfoil was discovered in Dudley Pond, this is the first time that the pond has been milfoil-free at the end of a season

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 PROBLEMS, HANDICAPS, & ISSUES Marking: ‘Seeing’ conditions for marking were not always optimal especially 6-8 weeks after the spot treatment.

Temperature: Water temperature limits the time a diver can be submerged, the difficulty of manipulation and pulling, and the ability to attract divers at all.

Milfoil grows in spring and fall when water temperatures make it impractical to dive in a wet suit. WSWQC may consider acquiring one or two ‘dry suits’ which would extend the hand-pulling season.

Unusual Algal growth: Divers reported silvery flecks in the water, limiting visibility. An algal mat formed in the cove near Rocky Point. Because the divers had not seen these types of algae before, we retained Dr. Ken Wagner, a prominent aquatic biologist to identify the samples and assure diver and public safety.

Dr. Wagner identified the algae and found no safety hazard, but commented that algae indicated high nutrient levels in Dudley Pond (See Appendix A)

Equipment reliability: A power regulator failed on one circulator and on another wires were chewed or cut. Repairs will be required.

Diver Productivity: Cost per plant harvested was up because the plants were fewer and farther apart – and because water clarity limited us at season end.

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 EFFECTIVENESS, OBSERVATIONS, LESSONS, CONCLUSIONS Neither Fluridone nor triclopyr were detected in Happy Hollow water sample. In 2010 we again took samples of water from the Happy Hollow wells, and sent them to SePRO for testing. The wells were off-line in spring and summer, so the water sample was taken in the fall No detectible concentration of fluridone nor triclopyr was found. We will continue this testing in 2011.

Submersible ‘scooters’ In 2009 we tested two electric-powered, recreational scooters. In 2010 these were helpful during the end of season surveying, and also allowed divers to cover larger areas of more widely distributed plants.

Triclopyr Highly Effective As ACT’s report indicates, the triclopyr flake spot treatment of Dudley Pond was very successful. Triclopyr is a systemic herbicide which, like fluridone, attempts to kill or suppress the plant. Triclopyr was highly selective and very effective with no adverse (and some positive) effects on native species. The flake formulation concentrates its effects in the roots. It does enter the water column and is detectable throughout the lake, but the effects are strongest at the bottom near the plants. Other species (notably curly leaf pondweed, and najas) were not affected by the herbicide.

Herbicide use likely unnecessary in 2011 We expect regrowth in 2011 but because the plants were so thoroughly suppressed, it is likely that only hand pulling will be necessary. Our goal is to continue to reduce the viable root mass through hand-pulling and spot treatment when and where necessary. We have made good progress away from a boom-bust cycle toward year to year management and herbicide minimization.

Unexpected Water Clarity Often with high nutrient levels, spot treatments can reduce competition for nutrients – and provide additional nutrients from decaying treated plants. This frequently causes algae blooms. We did see somewhat diminished water clarity since 2009, and noted algal growth – but there were no significant algal blooms and water clarity remained good.

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2010 GOOD WATER CLARITY (SECCHI DEPTH) Water was reasonably clear in 2010 but not as clear as 2009. Jack Peters, a DPA member, took Secchi depth (the depth at which the pattern on a painted 9� disk disappears) and water temperature readings.

An increase in water clarity over the summer may have allowed more light to reach lower depths stimulating plant growth in deeper areas. Clarity is normally higher when water is colder at the start and end of the season when there is less algae in the water column.

Water clarity is claimed to be an effect of water circulators, but WSWQC has no evidence to suggest that is the case in Dudley Pond.

2009 Secchi depth (ft) 25.0 20.0 15.0 Secchi depth (ft)

10.0 5.0 0.0 05/02/09 06/02/09 07/02/09 08/02/09 09/02/09 10/02/09 11/02/09

2010 Secchi depth (ft) 25.00 20.00 15.00 10.00

depth

5.00 0.00 3/16/10 4/16/10 5/16/10 6/16/10 7/16/10 8/16/10 9/16/10 10/16/10 11/16/10

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

2011 SEASON PLANS Well Sampling: We will continue annual spring tests of water from the Happy Hollow wells for fluridone. Samples will be analyzed by SePRO, and copies of test reports will be provided to the Conservation Commission and the DPW. Hand-pulling: Hand-pulling will continue at the same level as in 2010. We don’t know what to expect at the start of the season. We are looking for ways to make our divers more efficient and for them to work earlier and later in the season when the water is too cold for wet suits.

Spot treatment: We will investigate identified troublesome areas for potential spot treatment, but do not anticipate the need for herbicides in 201. Should we decide that spot treatment is required, we will notify the Conservation Administrator as required by our Order of Conditions.

Water Quality Testing: We are defining a periodic water quality sampling and testing plans to begin in Dudley Pond in 2011.

Research & actions to reduce nutrients Suppressing milfoil is not the same as reducing the high nutrient levels that support milfoil growth. Dudley Pond is still a category 5 (most impaired) due to high nutrient levels. The WSWQC is participating in the Dudley Area Study Committee, and is developing a household-level model to identify likely highnutrient contributions. WSWQC is also investigating improvements to Dudley Pond storm water management (notably iron-enhanced sand filters).

2011 EXPECTED COSTS & FUNDING WSWQC has budgeted $16,000 for Dudley Pond hand-pulling during the 2011 season.

We have a small contingency in our budget for a possible spot treatment.

The Dudley Pond Association is expected to continue to provide a 25% match of monies spent suppressing milfoil in Dudley Pond, reducing actual Town expenses by approximately $4000.

Wayland Surface Water Quality Committee

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Dudley Pond – DEP 332-698 2010 End of Season Report

APPENDICES A. Aquatic Control Technologies: Year-End Report for 2010 Renovate OTF Herbicide Treatment Program at Dudley Pond.

B. Dr. Ken Wagner – Algal test results C. 2010 Dudley Pond Diver Hand Pulling logs

Wayland Surface Water Quality Committee

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APPENDIX -A-

November 3, 2010

Wayland Surface Water Quality Committee Attn: Michael P. Lowery, Project Manager Wayland Town Building

41 Cochituate Road Wayland, MA 01778‐2614

Re: Year‐End Report for 2010 Renovate OTF Herbicide Treatment Program at Dudley Pond

Dear Mr. Lowery:

Please accept the following as our year‐end report for the Renovate OTF herbicide spot‐treatment program performed at Dudley Pond in 2010 for control of Eurasian watermilfoil (Myriophyllum spicatum). The treatment was performed in accordance with the product label directions, conditions of the Order of Conditions and Chapter 194 Permit for Dudley Pond (DEP 332‐698) issued by the Wayland Conservation Commission, and the License to Apply Chemicals (No. 10207) issued by MA DEP. The following report details the treatment program and monitoring tasks conducted by Aquatic Control Technology, Inc. during the 2010 season.

Introduction and Management History

Dudley is a 90‐acre waterbody located in the Town of Wayland, Massachusetts. Eurasian watermilfoil (milfoil) was first confirmed in Dudley Pond in 1991 and several herbicide treatments and non‐chemical control strategies have been employed over the past two decades to manage the infestation.

In 2008, the Wayland Surface Water Quality Committee (WSWQC) prepared a long‐term milfoil management plan that was initiated with a whole‐pond Sonar (fluridone) herbicide treatment program during the 2008 season. A concerted diver hand‐pulling effort was coordinated by WSWQC to remove milfoil root crowns in the late summer and fall of 2008. Despite continuation of an extensive monitoring and diver hand‐pulling program in 2009, a significant and unexpected amount of regrowth was reported by the WSWQC by the end of the 2009 season. After evaluating management alternatives, the WSWQC decided to proceed with a spot‐treatment program using Renovate OTF herbicide in 2010 to control areas with the most extensive milfoil growth. Initially, four locations totaling approximately 15 acres were identified as primary treatment areas and other two locations totaling approximately 3.5 acres were identified as secondary treatment areas.

2010 Treatment Program Chronology A chronology of the 2010 treatment program is provided below:

Order of Conditions and Chapter 194 Permit compliance .........................................(handled by WSWQC 2009‐2010) Pre‐treatment inspection...........................................................................................................................................5/7 DEP License to Apply Chemicals issuance (# 10207) ................................................................................................5/12 Renovate OTF Herbicide Treatment.........................................................................................................................5/19 Triclopyr Herbicide residue monitoring (4 rounds collected by WSWQC) .....................................6/5, 6/21, 7/11, 8/30 Late season survey...................................................................................................................................................9/24

Aquatic Control Technology, Inc. 11 John Road ● Sutton, MA 01590-2509 ● (508) 865-1000 ● Fax (508) 865-1220 ● info@aquaticcontroltech.com

APPENDIX -A-

2010 Dudley Pond Renovate OTF Treatment Program Report

2010 Treatment Program Protocol Treatment areas (Figure 1) were finalized following the pre‐treatment survey performed on May 7th. Six areas totaling approximately 18.5 acres were targeted for treatment. Based on the morphology of treatment areas Renovate OTF was selected for use in 2010. Renovate OTF granular was used to minimize dilution and maximize herbicide contact time in these areas. Renovate OTF was applied at 2.0‐2.25 ppm based on the bottom four feet of the water column. By mutual agreement between Aquatic Control Technology and the WSWQC, exposed treatment areas less than 2 acres (Areas 1 and 4) were expanded to approximately 2 acres to further overcome the effects of dilution and maintain adequate concentration‐exposure‐time. Area 2 (1.46 acres) was not expanded since it was located within a shallow and confined cove area.

Table 1: Final Renovate OTF Dosing Area

Acres

Recommended Dose

Pounds of OTF applied

1 2 3 4 5 6 TOTAL

2 1.46 4.94 2 3.25 4.84 18.5

245 215 215 215 215 215

490 315 1065 430 700 1040 4040

WSWQC applied sandbags to the outlet structure prior to treatment to prevent any outflow. On July 5th, nearly 45 days post‐treatment, WSWQC reported that the water level was down approximately 6 inches from the day of treatment and no outflow had occurred.

Summary of 2010 Treatment

Milfoil plants were observed to have more than 3‐4 feet of active growth at the time of the May 7th pre‐treatment inspection. A treatment date of May 19th was selected to allow enough time to comply with the notification requirements of the Wayland Order of Conditions and Chapter 194 permit.

Weather conditions on the day of treatment were overcast with periods of drizzle and light rain. The air temperature was approximately 50° F. Wind was out of the west/northwest, estimated at 5‐10 mph and did not interfere with treatment. Prior to treatment, water temperature was measured using a YSI Temperature/Dissolved Oxygen meter near the deep‐hole location

Table 2: Temperature/Dissolved Oxygen Profile on 5/19/10 Depth (m)

Temperature (°C)

Dissolved Oxygen (mg/L).

Surface 1 2 3 4 5 6

17.5 17.3 17.5 17.5 16.4 15.1 12.2

8.90 8.73 8.72 8.72 8.56 8.45 7.40

Water clarity was measured to be approximately 4.25 meters (14 feet) using a Secchi disk. The pH of the surface water was measured at 7.5 using a Hach colorimeter.

The treatment was using an airboat equipped with a granular eductor spray system that fed the granular herbicide into a stream of water using a calibrated venturi‐type eductor. The mixture was then sprayed off the stern of the boat using fan‐pattern nozzles. A Differential GPS navigation system with the treatment areas pre‐loaded was 2

APPENDIX -A-

2010 Dudley Pond Renovate OTF Treatment Program Report

used to insure that the herbicide was evenly applied to the designated treatment areas. A total of 4040 pounds of Renovate OTF (granular) were applied to the designated treatment areas. Actual passes of the treatment boat were recorded with the DGPS unit and are depicted in Figure 1. The herbicide began at approximately 8:25 a.m. and was completed at 11:55 a.m. The treatment was completed in approximately 3.5 hours.

Herbicide Residue Testing Summary Following the treatment, water samples were collected from Dudley Pond by WSWQC for analysis of the remaining in‐pond triclopyr concentrations. A copy of a summary table and chart prepared by M. Lowery of the WSWQC is provided in the Appendix, along with copies of the actual laboratory reports.

The theoretical lake‐wide triclopyr concentration from the 4040 pounds that were applied was 212 ppb. It is unlikely that this concentration was ever present in Dudley Pond at any one time, since triclopyr is released off of the granule over a period of several hours or days. Sixteen days after treatment in‐pond concentration was 97 ppb. At 32 days post‐treatment the concentration had dropped to 40 ppb and at 52 days post‐treatment the concentration was at 52 ppb. Even at 101 days post‐treatment there were still trace levels of fluridone (4 ppb) remaining in Dudley Pond. Triclopyr experienced roughly a 2‐week half‐life in Dudley Pond.

According to the Renovate OTF product label, treated water can be used for potable purposes once the concentration drops below 0.4 ppm (400 ppb). The only water use restriction associated with concentrations less than 400 ppb is not to use treated water for irrigation. This restriction does not apply to established grasses. A label change is pending that will allow for some greater flexibility in the use of treated water for irrigation.

In addition to the in‐pond sampling, WSWQC collected annual samples from the Happy Hollow wells for analysis of triclopyr and fluridone (from the 2008 Sonar treatment) residues on October 14th. Neither herbicide was detected.

Observations of Impacts to Aquatic Plants Approximately 12 days post‐treatment, WSWQC reported that the milfoil plants appeared to be distressed but there was still significant biomass in the water column. The plants reported continued to dieback slowly over the next several weeks. On September 14th, a diver survey from WSWQC reported that no milfoil was found.

Aquatic Control Technology performed its late season survey with M. Lowery of the WSWQC on September 24th.

The majority of the lake perimeter was toured by boat. No milfoil was encountered during the visual inspection or in several rake‐tosses. Non‐target plants that were observed during the survey included:

Table 3: Aquatic plants observed during late‐season survey Species

common name

type

Nitella Najas flexilis Vallisneria americana Potamogeton crispus Chlorophyta Nymphaea odorata

stonewort slender naiad wild celery curlyleaf pondweed filamentous green algae white waterlily

macro‐algae submersed submersed submersed (invasive) algae floating‐leaf

Any impacts to non‐target native plants were not evident by the time the late season survey was performed on September 24th. Some impact to other dicot plants, namely white waterlily, probably occurred immediately after treatment, but the triclopyr concentration was not lethal and the plants recovered by the end of the year. As anticipated, no impact was seen on the other invasive plant in Dudley Pond, curlyleaf pondweed, which is a monocot.

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APPENDIX -A-

2010 Dudley Pond Renovate OTF Treatment Program Report

Summary and Ongoing Management Recommendations

Based on observations by the WSWQC and those made by Aquatic Control Technology on September 24th, the 2010 Renovate OTF herbicide treatment program appears to have achieved complete control of Eurasian watermilfoil. Treating nearly 20% of the pond area and having no outflow for several weeks post‐treatment, probably resulted in prolonged exposure to lethal concentrations of triclopyr, which achieved pond‐wide milfoil control. Some widely scattered milfoil regrowth may be seen in 2011, but will likely become more evident in 2012. Based on past experience at Dudley Pond and in other Northeast waterbodies, if the milfoil is allowed to recover and grow unmanaged, it may reach pre‐treatment densities within 3‐4 years.

WSWQC now has a distinct advantage over the previous treatment programs performed at Dudley Pond, having direct experience and a clear understanding of the effectiveness and limitations of Renovate (triclopyr) herbicide spot‐ treatments, DASH/suction‐harvesting, and diver hand‐pulling. We still feel that an aggressive, integrated management program targeting selective milfoil control will afford the most effective means of preserving desired open‐water conditions and extending the duration of control in Dudley Pond. However, decisions on future management strategies need to be made once the distribution and density of milfoil regrowth can be identified and the cost‐effectiveness of possible techniques can be evaluated. Managing milfoil before it has an opportunity to recover and reestablish its root structures may be a key factor in extending the duration of control.

Specific recommendations for 2011 include:

Regular volunteer monitoring of the lake to identify and document any milfoil regrowth. Locations of any milfoil plants should be recorded using a GPS (if possible) or carefully translated to a scaled‐map to facilitate relocation for follow‐up management efforts. It may also be helpful to physically mark milfoil growth using small buoys as WSWQC has done in prior years.

Develop a management strategy criteria based on milfoil density, distribution and location of growth within the pond. This should help guide decision‐making for future milfoil management expenditures.

Be prepared to implement non‐chemical controls in 2011, as deemed to be appropriate and cost‐effective.

Plan for more substantial non‐chemical controls or herbicide spot‐treatments in 2012, as required.

We trust that this report adequately summarizes the 2010 Renovate OTF herbicide treatment program at Dudley Pond.

We thank Mike Lowery and the rest of the WSWQC for their assistance and oversight on this project and commend you all on your commitment to managing invasive Eurasian watermilfoil growth in Dudley Pond. Please feel free to contact us if you have any questions or require additional information at this time.

Sincerely,

AQUATIC CONTROL TECHNOLOGY, INC.

Marc Bellaud

Gerald N. Smith Senior Biologist

President/Aquatic Biologist

Enclosures: Appendix

4

APPENDIX -A-

2010 Dudley Pond Renovate OTF Treatment Program Report

APPENDIX

Figure 1 Dudley Pond Renovate OTF Treatment Areas

Herbicide Residue Data Summary Table and Chart of Results: prepared by M. Lowery Laboratory Reports

5

APPENDIX -A-

²

4

2 3

1 6 5

0

100 200

400

600

800

1,000 Feet

Legend:

Planned treatment areas (1=2.00 ac; 2=1.46 ac; 3=4.94 ac; 4=2.00 ac; 5=3.25 ac; 6=4.84 ac)

Dudley Pond Wayland, MA

Actual pass of treatment boat on 5/19/10 recorded by DGPS

2010 Renovate OTF Treatment Areas

bathymetric contour lines, 2-foot intervals

FIGURE:

SURVEY DATE:

MAP DATE:

1

05/19/10

11/03/10

Orthophoto base map source: MassGIS

11 JOHN ROAD SUTTON, MASSACHUSETTS 01590 PHONE: (508) 865-1000 FAX: (508) 865-1220 WEB: WWW.AQUATICCONTROLTECH.COM

APPENDIX -A-

DATE 05/19/10 06/05/10 06/21/10 07/11/10 08/30/10 09/19/10

ppb 212 97 40 11 4

interval

days since treatment

%orig 16 16 20 49

45.75% 18.87% 5.19% 1.89%

16 32 52 101 120

ppb 250

200

150

ppb

100

Prepared by: M. Lowery, WSWQC

08/25…

08/18…

08/11…

08/04…

07/28…

07/21…

07/14…

07/07…

06/30…

06/23…

06/16…

06/09…

06/02…

05/26…

0

05/19…

50

APPENDIX -A-

Chain of Custody C98875FF-D Customer Company Company Name:

Customer Contact Aquatic Control Technology, Inc.

Contact Person:

Gerald N gnsmith@aquaticcontroltech.com

Address:

11 John Road

E-mail Address:

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

Date Treated

Date Sample Collected

Products

Acres Treated

Rate

Active

Result

1

05/19/2010

06/05/2010

Renovate OTF

18.5

4036

Triclopyr

0.097 ppm

Laboratory Information Date Received:

6/10/2010

Date Analysis Performed:

6/10/2010

Date Results Sent:

6/10/2010

Storage Conditions

Analyzed Immediately

APPENDIX -A-

Chain of Custody 271E581F-7 Customer Company Company Name:

Customer Contact Aquatic Control Technology, Inc.

Contact Person:

Gerald N gnsmith@aquaticcontroltech.com

Address:

11 John Road

E-mail Address:

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

1

Date Treated 05/19/2010

Date Sample

Sample Location

Products

Collected 06/21/2010

Renovate OTF

Acres Treated 18.5

Rate

Active

Result

4036

Triclopyr

0.040 ppm

Laboratory Information Date Received:

6/24/2010

Date Analysis Performed:

6/24/2010

Date Results Sent:

6/24/2010

Storage Conditions

Analyzed Immediately

APPENDIX -A-

Chain of Custody 6D66DB90-6 Customer Company

Customer Contact

Company Name:

Aquatic Control Technology, Inc.

Contact Person:

Gerald N

Address:

11 John Road

E-mail Address:

gnsmith@aquaticcontroltech.com

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

1

Date Treated 05/19/2010

Date Sample

Sample Location

Products

Collected 07/11/2010

Renovate OTF

Acres Treated 18.5

Rate

Active

Result

4036

Triclopyr

0.011 ppm

Laboratory Information Date Received:

7/13/2010

Date Analysis Performed:

7/14/2010

Date Results Sent:

7/14/2010

Storage Conditions

Analyzed Immediately

APPENDIX -A-

Chain of Custody 0E00D8BA-7 Customer Company

Customer Contact

Company Name:

Aquatic Control Technology, Inc.

Contact Person:

Gerald N

Address:

11 John Road

E-mail Address:

gnsmith@aquaticcontroltech.com

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

1

Date Treated 05/19/2010

Date Sample

Sample Location

Products

Collected 08/30/2010

Renovate OTF

Acres Treated 18.5

Rate

Active

Result

4036

Triclopyr

0.004 ppm

Laboratory Information Date Received:

9/1/2010

Date Analysis Performed:

9/1/2010

Date Results Sent:

9/1/2010

Storage Conditions

Analyzed Immediately

APPENDIX -A-

Chain of Custody EB0F7C6E-7 Customer Company

Customer Contact

Company Name:

Aquatic Control Technology, Inc.

Contact Person:

Gerald N

Address:

11 John Road

E-mail Address:

gnsmith@aquaticcontroltech.com

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

Date Treated

Date Sample

Sample Location

Products

Acres Treated

Rate

Active

Result

Collected

HH1

05/02/2010

10/14/2010

Renovate 3

18.5

4036

Triclopyr

0.000 ppm

HH2

05/02/2010

10/14/2010

Renovate 3

18.5

4036

Triclopyr

0.000 ppm

Laboratory Information Date Received:

10/20/2010

Date Analysis Performed:

10/20/2010

Date Results Sent:

10/20/2010

Storage Conditions

Analyzed Immediately

APPENDIX -A-

Chain of Custody E580862B-5 Customer Company

Customer Contact

Company Name:

Aquatic Control Technology, Inc.

Contact Person:

Gerald N

Address:

11 John Road

E-mail Address:

gnsmith@aquaticcontroltech.com

City:

Sutton

Phone:

State:

MA 01590-2509

Fax:

Invoice

Card Number/Expiration Num:

Waterbody:

Dudley Pond

Waterbody Size (acres):

Depth Average:

8.00

Target Plants

Eurasian Watermilfoil,

Payment Information Payment Type:

Waterbody Information 86.00

Sample Information

Sample Site ID

Date Treated

Date Sample

Sample Location

Products

Acres Treated

Rate

Active

Result

Collected

HH1

07/31/2008

10/14/2010

Wayland Municipal

Sonar A.S.

86

12

Fluridone

<1.0 ppb

HH2

07/31/2008

10/14/2010

drinking water wells

Sonar A.S.

86

12

Fluridone

<1.0 ppb

Laboratory Information Date Received:

10/18/2010

Date Analysis Performed:

10/18/2010

Date Results Sent:

10/18/2010

Storage Conditions

Analyzed Immediately

08/04/2010

APPENDIX -B-

Gmail - algae results

Mike Lowery <lowery.mike@gmail.com>

algae results Ken Wagner <kjwagner@charter.net> To: lowery.mike@gmail.com

Fri, Jul 23, 2010 at 12:05 AM

Have had a tough day with computers. Lost all past emails and contacts. Fortunately, I had just completed creating a file of most contacts, and you were in it, so I have your email. However, I got a nice email from Ted Fuist and have now lost it. Please let him know – if he can resend, that will work.

Attached are 3 Excel files with the results from the 3 samples. 3 very different samples. Sample A had mostly small green algae, motile Volvocales (an Order of algae) and Chlorococcales, high enough density to be noticeable, but not really excessive. Generally associated with higher nitrogen levels. B had just Mougeotia, a slimy filamentous green alga in the same group with Spirogyra and commonly found in lakes with active exchange of P at the sediment-water interface. Many “cleaner” lakes that have sequestered inputs in the sediments for years will get such growths in the spring and early summer in shallow water. Lakes with more nutrients may support these growths through summer, and they can appear as clouds in the water as they rise off the sediment. C had mostly Plectonema, a thick, dense, tough blue-green filamentous form, with lesser amounts of closely related Lyngbya and the normally planktonic Coelospharium and Microcystis. Also had some Botryococcus, and unusual green alga that stores oil instead of starch. These are all indicators of high nutrient levels, no significant nitrogen limitation, and could make the surface of the pond unpleasant over time. The Plectonema needs a long growing season to get dense enough to trap its own gases and float up; seeing surface mats now is early for this genus.

To some degree, these algae could all be linked to the weed treatments; decaying vegetation can provide the impetus for algal growth in the year of treatment. A bit of a tradeoff. On the other hand, what I saw in B and C could recurr if the sediment is nutrient rich and actively releasing those nutrients, which is a distinct possibility independent of the weed treatments. The mats are not especially treatable with algaecides; they inhibit exposure to chemicals. And the small greens in the Order Chlorococcales are fairly resistant to copper as well, mostly due to gelatinous sheaths. The hot sunny weather is undoubtedly aiding growth, too.

Have to be in the field tomorrow, but welcome to try to reach me by cell phone. Around over the weekend and most of Monday, then pretty scarce the rest of next week. Invoice also attached.

Ken Wagner Water Resources Services 413-219-8071 kjwagner@charter.net

4 attachments Dudley Pond A 072010.xls 26K https://mail.google.com/mail/?ui=2&ik…

1/2

08/04/2010

APPENDIX -B-

Gmail - algae results

Dudley Pond B 072010.xls 26K Dudley Pond C 072010.xls 28K Dudley 072210.doc 23K

https://mail.google.com/mail/?ui=2&ikâ&#x20AC;Ś

2/2

APPENDIX -C-

2010 Dudley Pond Hand Pulling

Crew/Name Allison LaClaire Allison LaClaire Allison LaClaire Ted Fiust Allison LaClaire Allison LaClaire Ted Fiust Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire Noreen McDonnel Shawn Loisean Trevor Pitts Trevor Pitts Noreen McDonnel Shawn Loisean Ted Fiust Trevor Pitts Noreen McDonnel Shawn Loisean Ted Fiust Trevor Pitts Allison LaClaire Noreen McDonnel Shawn Loisean Ted Fiust Ted Fiust Allison LaClaire Allison LaClaire Allison LaClaire Noreen McDonnel Shawn Loisean Trevor Pitts Noreen McDonnel Noreen McDonnel Shawn Loisean Trevor Pitts Noreen McDonnel Shawn Loisean Ted Fiust Ted Fiust Trevor Pitts Noreen McDonnel Shawn Loisean

WSWQC

Activity type Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Snorkler Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Diver-hand Boat Diver-hand Diver-hand Diver-hand

Month APR MAY MAY JUN MAY MAY MAY MAY MAY MAY MAY MAY JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN JUN

Date 4/26/2010 5/1/2010 5/1/2010 5/1/2010 5/2/2010 5/4/2010 5/24/2010 5/25/2010 5/25/2010 5/26/2010 5/27/2010 5/31/2010 6/2/2010 6/3/2010 6/3/2010 6/3/2010 6/3/2010 6/3/2010 6/3/2010 6/4/2010 6/4/2010 6/4/2010 6/4/2010 6/5/2010 6/5/2010 6/5/2010 6/5/2010 6/7/2010 6/7/2010 6/7/2010 6/7/2010 6/7/2010 6/8/2010 6/8/2010 6/8/2010 6/8/2010 6/8/2010 6/8/2010 6/9/2010 6/9/2010 6/9/2010 6/9/2010 6/12/2010 6/12/2010 6/12/2010 6/12/2010 6/12/2010 6/14/2010 6/14/2010

Plants Pulled 12 23 11 123 54 157 205 23 165 111 202 135 47 110 112 260 250 240 240 250 220 120 175 97 75 50 75 28 50 221 54 54 6 3 2 170 325 160 150 150 150 150 155 175 50 150 75 182

Hours Harvested Worked Area 1.15 1E 1 1E 1 3B 1.45 1E 1.5 3B 3 2B 2.5 1A 0.5 1E 2 3B 2 3B 3 3B 2 1E 1 3B 2 3C 2 3B 8 2B 8 2B 8 2B 8 2B 9 2B 9 2B 4 2B 9 2B 9 1A 9 1A 9 1A 9 1A 2 2F 8 2D 8 2D 1.5 2G 1.5 1F 1 2E 0.3 2G 0.3 3C 8.5 3C 8.5 3C 9 3C 8 1A 8.5 1A 8 1A 8 1A 8 4E 8 4E 8 4E 8 1A 8 4E 5 2A 5 2A

Water Temp (f) 53.00 62.00 62.00

Depth Locale, Direction Comments (ft) 4 Swam from my shore (9 Priscilla Path) 1-3 ft vis, light wind, cloudy 4 swam from my house on a 80 degree air temp and sunny.

4 Swam from my shore line southwest to Picked 6 >10 stems and 5 >20 stems

64.00 64.00 67.00 67.00

4 5 7 4 4 4 5 5 5 7 5

Paddled out to work in the northeast 2B-2C-2D intersection though spent Mike, Swam from my shore southwest to ~30 On the eastern edge of 3B. swam from Continue to work the eastern line of 3B Started on the northeastern side of 3B Outlet, then swam to the north side of just southeast of the center of 3B right on the eastern edge of 3C and 3B center of 3B swimming south ~50m

Windy, warm, sunny.

1-3 ft vis It appears unrealistic to try to keep a count of big vs 1-3 ft vis continues. MF (6 days post tryclopyr tx) 90 and sunny. Warm calm water w/ pollen coating the 90 degrees and sunny...hot.

65 air degrees and partly sunny to sunny. Light wind. vis 1-2ft (poor), windy and 74 air degrees. Smoky hazy 75 air degrees, windy poor vis in the top ft of water column but clears to mod 75 air temp, windy, partly cloudy and humid. Vis was

6

Grace's Cove-the whole cove.

vis low/poor ~1-2ft . 28 large plants scattered around

6

still don't have a map to reference when Mf is extemely fragile. ...even in Chat cove. Much of the

8 4 8

Swam 2/3 of 2E starting from the The whole cove Swam from 2G cove next to the island

Poor vis ~1ft w/ lots of particles in the water column. poor vis. Challenge getting through lilies, though they Again, finding little. What was found was big but The name is wrong but you need to add Shawn

Divers worked in Mansion Beach area

1

Divers found mostly dying plants that had stems near

APPENDIX -C-

2010 Dudley Pond Hand Pulling

Crew/Name Ted Fiust Ted Fiust Trevor Pitts Ted Fiust Trevor Pitts Allison LaClaire Allison LaClaire Allison LaClaire Allison LaClaire

WSWQC

Activity Month type Diver-hand JUN Boat JUN Support JUN Boat JUN Diver-hand JUN Diver-hand SEP Diver-hand SEP Diver-hand SEP Diver-hand SEP

Date 6/14/2010 6/14/2010 6/14/2010 6/15/2010 6/15/2010 9/13/2010 9/14/2010 9/21/2010 9/22/2010

Plants Pulled 33 0 110 0 0 0 0

Hours Worked 5 5 5 8 8 2.5 2 2.5 2.5

2010

6,145

287.2 $11,488.00 $1.87

2009

18,000

358 $ 14,320.00

Harvested Area 2A 1A 2A 1A 1A 2D 1A 3C 3B

Water Temp (f)

Depth (ft)

67.00 67.00 66.00 67.00

6 7 10 5

hours cost per plant

hours cost

2

Locale, Direction

Surveyed The whole Chateau side of 1A and 3C to 2G: Started in 2A and swam south to 1B 2G-Boated the edges then swam the

Comments

Mostly najas (2-3 ft thick). Curley leaf coming back in NO MILFOIL NO MILFOIL NO MILFOIL!!