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ProcedureEnsureconsistencyofpracticetopreserveconditionandavailabilityofpatient’sveinsProcedureEnsureconsistencyofpracticetopreservecondition andavailabilityofpatient’sveins.Invenousbloodsampling,aneedleisinsertedintoaveintocollectasampleofbloodfortesting.Invenousbloodsampling,a needleisinsertedintoaveintocollectasampleofSubjectOnlypersonnelcompetentinvenipunctureshouldperformthisprocedureThisSOPdescribesthe proceduresforresearcherstoperformthecontrolledact,performingaprocedureontissuebelowthedermis,bycollectingvenousbloodThischaptercoversall thestepsrecommendedforsafephlebotomyandreiteratestheacceptedprinciplesforblooddrawingandbloodcollection(31)Performingaprocedureontissue belowthedermisisconsideredacontrolledactWarningsandCommonErrorsPurposePolicyThisSOPdescribestheproceduresforphlebotomiststoStepby-StepDescriptionofProcedureEnsureaccurateresultsthroughcollectionofqualitybloodspecimenLineuptheneedleandsyringewiththeveinfromwhich thebloodwillbedrawn.Thephlebotomist’sthumbshouldbeoneortwoinches(cmorcm)belowthevenipuncturesite.Performingaprocedureontissuebelow thedermisisconsideredacontrolledact.BLOODCOLLECTION.Aftercare.forbloodcultures)orothertheneedleinthevein.BLOODCOLLECTIONBY VENIPUNCTUREPurposeIfnobloodflows,slowlyretractoradvancetheneedleuntilbloodappearsRemoveThisstandardprovidesproceduresforthe collectionofdiagnosticvenousbloodspecimens,includinglinedraws,bloodculturecollection,andvenipunctureinchildrenBLOODCOLLECTIONBY VENIPUNCTUREThechapterincludesbackgroundinformation(Section),practicalguidance(Section)andillustrations(Section)relevanttobestpracticesin phlebotomyAPURPOSEANDBACKGROUNDAftercareIdentifythePatientThisanchorstheveinTurntheneedlesothebevelsideisinanupward positionBloodshouldimmediatelybegintofillthetubeduetovacuumactionTipsandTricksPolicyThisSOPdescribestheproceduresforphlebotomiststo collectvenousbloodsamplesusinganovertheneedlecatheterfromadultstudyparticipants(i.e.,ageandolder)Step-by-StepDescriptionofProcedure. Peripheralveins,typicallytheantecubitalveins,aretheusualsitesforvenousbloodsamplingSubjectPushtheneedleintotheveinThechapterincludesSpecimen collectionmethodsincludevenipuncture,wherebloodiscollectedbypenetratingaveinwithaneedleandacollectionapparatusorsyringe,andbytheskinA PURPOSEANDBACKGROUNDWarningsandCommonErrorsTipsandTricksAstandardforglobalapplicationdevelopedthroughtheClinicaland LaboratoryStandardsInstituteconsensusprocessWHOLibraryCataloguing-in-PublicationDataWHOguidelinesondrawingblood:bestpracticesin phlebotomyBloodletting–standardsPhlebotomy–standardsNeedlestickinjuries–preventionandSpecimencollectionmethodsincludevenipuncture,whereblood iscollectedbypenetratingaveinwithaneedleandacollectionapparatusorsyringe,andbytheskinordermalmethod,whichinvolvesthecollectionofcapillary bloodafteranincisionismadeintheskinwithalancetThischaptercoversallthestepsrecommendedforsafephlebotomyandreiteratestheacceptedprinciples forblooddrawingandbloodcollection(31)EnsureSerum-GelRoutinely,venousbloodcollectionisthemethodofchoicewhenevermajorbloodvolumesare requiredforlaboratorydiagnostics(eg