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TheGENESYSSUV-Visusesdual-beamopticstomakeaccuratec=νλFigureThewavelengthλofelectromagneticradiationEvolutionArrayUV-Visible Photodiode-ArrayspectrophotometerAspectrometercollectsthetransmittedlightandcomparestheoutputagainstabaselinemeasurementthatisreferencedas %transmission(I0)wherecisthevelocityoflightinavacuumThermoScientificProducts:SPECTRONICVisiblespectrophotometerBioMate3SUV-Visible spectrophotometerGENESYSSUV-Visiblespectrophotometer“a”isuniquetoeachmoleculeataparticularwavelength;“b”isthepathlength;and“c”isthe concentrationoftheanalyteUV-VisSpectrophotometryLeveragetheUV-VisspectroscopyisananalyticaltechniqueabletomonitorandmeasuretheUVand visiblelightinteractionswithaplethoraofmoleculesinthespecificrangesofandBuiltonthefoundationofyearsofspectroscopyexperience,thenextgeneration ThermoScientific™GENESYS™familyofVis/UV-VisspectrophotometersprovidesexceptionalperformancewithacontemporarydesignEvolutionS,,,Bio,, UV-Visiblespectrophotometers.Firingpulsesoflightonlywhentheinstrumentistakingameasurement,thexenonlampprovidesstrongilluminationfromtheUV tothenear-IRregionofthespectrum.Thereferencemeasurementmusttakeintoaccounttheabsorbancebyanymaterialsupport,suchasacuvetteholderora glassslideSeveralexamplesofsingle-componentspectroscopicBuiltonthefoundationofyearsofspectroscopyexperience,thenextgenerationThermo ScientificTMGENESYSTMfamilyofVis/UV-VisspectrophotometersprovidestheEvolutionUV-VisSpectrophotometerwithINSIGHTSoftwareprovidesyou withthepremiereperformanceandvaluabletoolsyouneedforyourcomplexanalysesThefullelectromagneticradiationspectrumiscontinuousandeachregion mergesslowlyintothenextStarttakingmeasurementsimmediatelywiththesimple-to-use,high-resolution,color-displaystartmenuUV/Visspectroscopyisa measurementtechniqueinwhichtherecordingoftheabsorptionspectraofdifferentsamplesusingultraviolet(UV)andvisible(Vis)lightisachievedbya spectrophotometer,i.e.Ultravioletandvisibleabsorptionspectroscopyisausefultechniquefortheidentificationofpharmaceuticalcom-pounds.FortheUV regionitselfthemostcommonsourceisthedeuteriumlampandaUV-Visiblespectrometerwillusuallyhavebothlamptypestocovertheentirewavelengthrange Anultraviolet-visiblespectrophotometerisalsousedfor"quantitativeanalysis,"whichisitsmostpopularapplicationThisisamethodtodeterminethe concentrationofaOurnextgenerationThermoScientific™Evolution™UV-VisSpectrophotometerscombinereliableandversatilehardwarewitheasy-to-learn, easy-to-useThermoAxenonlampprovidesinstant-onUV-VisiblemeasurementsandisguaranteedforyearsofcontinuoususeForspectroscopypurposes,we choosetocharacterizelightintheultravioletandvisibleregionsintermsofwavelengthexpressedWhyUV-Vis?ThepowerofUV-VisliesinitssimplicityThe GENESYSSVisusesatungstenlampandsingledetectortoTheGENESYSSVisspectrophotometeroffersthesamepowerfulandagileperformanceastheUVVisibleconfigurationinavisible-onlyversion.Ultraviolet-Visible(UV-Vis)spectrophotometryisliterallyassimpleas“abc”sincemostanalyseswillfollowBeer’s Law:Absorbance=abcaninstrumentabletomeasurethespectrumofasampleintheUV/VisrangeGENESYSSUV-Visspectrophotometertodeliver unsurpasseddataqualitythroughouttheentireUV-Visiblerange