raport ansvsa ppc

CLASSICAL SWINE FEVER IN ROMANIA SCFCAH - ANIMAL HEALTH AND WELFARE Brussels, 3-4th of February 2009

Summary Historical data CSF Romanian Programme in 2008 CSF Romanian Programme in 2009

Historical data Data about outbreaks 2005 - 1504 outbreaks of classical swine fever

2006 - 803 outbreaks of classical swine fever

2007 - 168 outbreaks of classical swine fever

2008 – none The last outbreak in backyards was declared on 04 May 2007 The last outbreak in commercial holdings (Igriş I Farm – Smithfield) was declared on 22 August 2007.

The last outbreak in free ranged domestic pigs was declared on 09 October 2007 in Insula Mică a Brăilei Natural Reservation.

Number of CSF outbreaks in domestic pigs 20012008 1600 1508 1400 1200 1000 803

800

number of outbreaks

600 400 200

180

155 1

0 1

168

45 2

0 3

4

5

6

7

8

Overview of the CSF situation in Romania during 2001- 2008 2001

2002

2003

2004

2005

2006

2007*,1

2008*

Non-

Commercial

prof.

farms

CSF outbreaks in domestic pigs

1

45

155

180

1508

803

165

3

0

Dead pigs due to CSF

13

452

1021

634

2982

1903

507

11.030

0

Destroyed pigs due to CSF

0

985

3592

1624

8045

5898

4.002

42.659

0

Total number of affected pigs

13

1437

4613

2258

11027

7801

4.509

53.689

0

Affected villages

1

45

155

161

482

393

95

2

0

Affected counties

1

17

16

26

33

35

19

1

0

CSF cases in wild boar

5

2

6

4

43

18

25

0

0

Emergency vaccination in 2007 Domestic pigs vaccination in non professional holdings Used vaccines: „ Vaccines from the Commission vaccine bank : - 1.009.750 doses Riemser - 1.000.000 doses Merial „ Vaccines purchased by Romania: - 9.896.473 doses Romania

Emergency vaccination in 2007 Domestic pigs vaccination in professional holdings Vaccines used: „ Vaccine from the Commission vaccine bank : - 7.050.000 marker doses

CSF – RO Programme in 2008 Commission Decision 2007/870/EC amended by Decision 2008/682/EC In addition to 2007, Romania forwarded during 2008 some additional clarifications: - a new NDCC and LDCC law - establishing the rearing systems for pigs and adopting the biosecurity measures; - establishing a network of specialists with exclusive competences in the control of CSF, comprised of: 42 specialists at county level, 8 at regional level and 1 at central level, with the role of supervising the implementation of the CSF eradication programme; - establishing Detailed Vaccine Control Procedures that can be applied fron transport, storing, distribution including trading.

Data regarding the vaccine used for implementing the program during 2008 Romania

Counties

Total number of animals

Information on vaccination programme

Number of herds(c) in vaccination programme

Number of vaccinated herds(c)

Number of vaccinated animals

Number of doses of vaccine admin

Number of vaccinated adults)

Number of young(d) animals vaccinated

2007

42

5,400,000

1,400,195

1,400,195

11.284.116

11.999.742

1954436

7995236

2008

42

6,000,000

1,519,876

1,519,876

7,231,002

7,622,831

287793

6,943,209

Emergency vaccination in 2008 Domestic pigs vaccination in non professional holdings Vaccine used: Vaccines purchased by Romania in 2008: - 5.100.000 doses Romania Vaccine supply in 2007: - 2.700.000 doses Romania Number of vaccinated pigs: - 7.231.002 pigs Stock Vaccine for 2009 - 1.700.000 doses

Serological surveillance in backyards 2007-2008 28 days after vaccination, 15 samples were taken for each village in order to establish post vaccination protection of pig population.

Serological surveillance of back yards in 2007(1) and 2008(2) 90000 88608 88000 86000

85195 number of tested samples

84000 82634

82207

82000 80000 78000 1

2

nomber of positive samples

Immunity in backyards The average of positive results after vaccination were 96% - that means a good immunity.

Level of immunity after vaccination in non comercial exploatation in 2008 (April to December) 98.5 98 97.5 97

Level of immunity after vaccination in back yard

96.5 96 95.5 95 1

2

3

4

5

6

7

8

9

The surveillance in Romanian non-professional holdings 2008 ELISA detection of post vaccine antibody

+

-

Doubt

85195

82207

2482

504

VIRUSOLOGICAL EXAM FAT Samples 774

IPO

+

_

0

774

RT-PCR

Samples 14

+

_

Samples

0

14

18

+

Virus isolation

18

0

Emergency vaccination in 2008

Domestic pigs vaccination in professional holdings „ Continued until April 2008 – marker vaccine (European vaccine bank 2007)

Surveillance in commercial farms 2008

Surveillance in commercial holdings - In case of positive result in ELISA marker were performed supplementary laboratory tests as RT-PCR and an epidemiological enquiry to rule out CSF. Most of the positive samples were taken from sows vaccinated with live vaccine.

- All the viral test were negative.

Surveillance of comercial farms in 2008 by:ELISA (1), RT-PCR(2) and FAT(3) 25000 20722 20000

15000 number of tested samples

11832

number of positive results 10000

5000 1560 136

0

0 1

2

0 3

Serology and RT-PCR on different age class in commercial holdings Serological and RT-PCR sampels on different age class: sows(1), boar(2), fattening pigs(3), weaning pigs(4), off-spring(5) 9000 7651

8000

7302

7000 6000

5071

5000 4000

3927 3554

RT-PCR on blood sampels

3000 1676 1419

2000 1000

745

706

3

4

423

0 1

ELISA tests

2

5

Emergency vaccination in 2008 Wild boar vaccination Vaccine baits used - 91.111 baits Hunting Founds - 929 Feeding grounds - 2.365

Estimated vaccinated population 42.188 boars The control for establishing the effectiveness of wild boars vaccination is still ongoing.

Surveillance during 2007-2008 wild boars hunting campaign WILD BOARS YOUNGER THAN 1 YEAR

WILD BOARS 1 – 2 YEARS OLD

WILD BOARS OF MORE THAN 2 YEARS

Serological exam

Serological exam

Serological exam

Virusologic al exam RTPCR+ FAT

Virusolgical exam RT-PCR +FAT

Virusological exam RT-PCR +FAT

No. samples

+

No. samples _

+

_

No. samples

+

_

No samples

+

_

No. samples

+

_

+ No. samples

_

2527

28

2499

2852

0

2852

2522

32

2489

2805

0

2805

4304

177

4127

0 4809

4809

Viral surveillance of wild boar 2007-2008 The graphic show that in 2008 the number of viral exams increased (12.7%) comparing with 2007.

Viral surveillance of wild boar in 2007(1) and 2008(2)

10400

0

10200 10000 9800 9600 9400 9200 9000

10285 25

8800 8600

8978

8400 8200 1

2

number of positive samples number of tested samples

Serological surveillance of wild boars 2007-2008

„

The graphic shows that in 2008 the number of serological exams increased comparing with 2007 and the number of positive results decreased.

Serological surveillance of wild boar in 2007(1) and 2008(2) 10000 9353

9000 8000 7468

7000 6000

no. of tested samples

5000

no.of positive samples

4000 3000 2000 1000 373

0 1

237 2

Serological surveillance in 2008 on different ages class The 27 serological positive samples from wild boar of 1 year age class were taken in first quarter of 2008. This means that the latest infection was at the end of 2007. One of positive samples from wild boar of 1 year age has origin in a hunting found where was performed vaccination.

- No positive results at this age class were detected in recent hunting season.

- We think that positive samples from this age class have epidemiological importance.

Se r ological s ur ve illance of w ild boar - diffe r e nt age clas s in 2008 (1= 1ye ar clas s ;2= 1-2 ye ar s clas s ;3=m or e than 2 ye ar age clas s ) 5000 4500

4304

4000 3500 3000 2500

2527

2522

28

32

no. of tested samples no. of positive samples

2000 1500 1000 500 0 1

2

177 3

Prevalence and incidence of CSF in wild boar population 2007-2008

In 2008 the prevalence of CSF in wild boar population is lower than in 2007.

In 2008 the incidence of CSF in wild boar population is zero – absence of viral positive results.

The prevalance and incidence of CSF in wild boar in 2007(1) and 2008(2)

6 5

0.27

4 3

Incidence of CSF in wild boar population 0

4.9

2 2.5 1 0 1

2

Prevalance of CSF in wild boar population

Genotyping of Romanian isolates 2005-2007

For genotyping the Romanian isolates, both the 5’ non translated region (5’-NTR) and the E2 glycoprotein gene were sequenced at CRL - Hannover, Friedrich-Loeffler Institutes and IDAH. The Romanian isolates form a highly homologus cluster within subgroup 2.3.

This cluster comprise isolates from different European countries like former Yugoslavia, Swityerland, Spain and other Eastern European countries.

In the 5’-NTR fragment, sequence identities between the Romanian isolates were 98.6 and 100%.

Following the programme performed from December 2006 – December 2008, the following results can be noticed: As a conclusion, the 2008 programme led to the following results: a) The significant reduction of the virus circulation and of the clinical signs; b) Significant, diminishing of the number of outbreaks; c) Reducing viral pressure on domestic and wild pigs; d) In Romania there were no cases of classical swine fever notified.

In the wild boar population the epidemiological situation was improved without vaccination.

CSF – RO Programme in 2009 For 2009, the programme is approved, by Commission Decision no.

897/2008 and regarding emergency vaccination the following is mentioned: Stopping domestic pig vaccination in commercial holdings; Continuation of vaccination in non professional holdings – whole territory; wild boar vaccination – whole territory .

The programme is approved until 31 decembrie 2009.

The Romanian export of pig meat and pig meat products is the final goal of Romania and for this purpose NSVSA was requested come up with a regionalization plan in order for Romania to comply with Annex III of Commission Decision 2008/855/CE, offering the possibility, in certain conditions, to export meat and meat products in “lohn� system.

ANSVSA reviewed the procedures for a possible approval of intracommunity trade with meat products and carried out the evaluation of the establishments that requested to be put on the list.

Following these evaluations only 3 establishments comply with the approval requirements. NSVFSA informed the Commission in order for the 3 establishments to be approved for “lohn� intracommunity trade.

Conclusions 1. The measures taken until now through vaccination in domestic pigs were compliant.

2. The programme approved for 2009 by Commision Decision 897/2008 is in compliance with the current situation of classical swine fever in Romania.

3. The strategy to stop vaccination of domestic pigs and a possible regionalization, represent measures to implement the European field, that need to be evaluated by involved, in order to comply with requirements.

the necessary legislation in this all stakeholders all EU specific

THANK YOU!