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“AN INNOVATIVE MODEL TO QUANTIFY CORNEAL TRANSPARENCY IN DONOR CORNEAL BUTTONS� BACKGROUND: Assessment of corneal transparency in donor corneal buttons has only been performed qualitatively. The quantification of corneal transparency has implications with respect to clinical suitability of the donor cornea and research involving corneal transparency as an outcome measure. We present a simple, reproducible cost effective model of quantifying corneal transparency. METHOD: An Artificial anterior chamber was modified to create a central transparent passage. This was made possible by replacing the base of the chamber with Perspex and a water tight seal. An inlet and outlet tube was attached to the sides of the chamber. This was done to maintain a standardized pressure within the chamber when connected to an IV bottle. A corneal button rejected for corneal transplant was placed on the artificial anterior chamber and the chamber was filled with normal saline. A digital camera and a laser source were placed on either side of the chamber at predetermined intervals. Alignment was achieved so that the laser light passed through the center of the cornea. The image of the laser spot was acquired. To test this proof of concept, a human donor cornea rejected for human transplant was injected with intrastromal saline to create corneal haze. Pre and Post injection images were analyzed. The average pixel intensity was calculated. RESULTS: The mean pixel intensity of the cornea prior to injury was 111 and post-saline injury was 17.2. CONCLUSION: Laser scatter quantification can be an effective tool to measure corneal transparency in donor corneal buttons. The simple inexpensive set up and ease of analysis are advantages of this method of quantification. Pre and post injury measurements showed a marked difference in average pixel intensity. A102
Study of Pupil Dilatation Characteristics in patients from South India with topical Tropicamide -Phenylephrine
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7th Annual Research Day