University of Southern Philippines Foundation
placed in an evaporating dish and heated over a water bath maintaining a temperature of 70 degrees to completely remove the solvent or until a dried substance was obtained. Further extraction like reflux distillation was employed, which is a technique used for the condensation of vapors and the return of the condensate the to system where it originated. A condensed amount was fitted to a Florence flask containing the macerated plant sample with water inlet provided in the condenser to allow constant flow of the water to cool the condenser. Confirmatory test for Hesperidin Hesperidin showed a color reaction with ferric chloride. With higher concentration, it produced a red wine color but with much concentration, the color became almost black. Preparation of White Ointment Base White ointment base was prepared using fusion method of which the ingredients were melted in descending points. Eight (8) grams of white wax were melted in the evaporating dish over a water bath. Sixteen grams of petrolatum were incorporated to the melted wax continuously stirring and was cooled, then transferred to a wide-mouth bottle. Preparation of the Test Animals All of the nine (9) male rabbits were acclimatized for a week before the experimentation, placed in separate cages and divided into three groups containing three rabbits each group namely: Experimental group treated with the Orange rind-pith extract; the Positive group treated with Hirudoid ointment and the negative group treated with the ointment base. Collection of Data The method used in gathering the data was single blinding of which the researchers measured without knowledge of what group the rabbit belonged. Using the tape measure, the marginal vein in the ear of the rabbit was the baseline data before inducing to inflammation. Measurements were recorded in the Record sheet No. 1 shown in Appendix A. After establishing the baseline data, the marginal vein in the ear of the rabbits were mechanically 16