What is Cell Clumping? It’s Causes, and The Technologies Used to Avoid it.

What is Cell Clumping? It’s Causes, and The Technologies Used to Avoid it.

Cell culture can be time-consuming and labour-intensive and a significant portion of your results will depend on following proper methods and using advanced technologies.

Cell culture has evolved into one of the most fundamental techniques for modeling biological systems, and it is becoming increasingly important in the biotechnology and pharmaceutical industries, as well as a necessary process in life science research labs.Cell Separation and culture are essential tools for the study of cell function.

Let's talk about one of the most serious cell culture issues: cell clumping, its causes, and how to overcome it using new technologies.

What is Cell Clumping?

It is not uncommon to see cell loss in a sample when working with singlesuspension cell cultures in a growth medium. When cells are ruptured, DNA and debris are released, causing cells to aggregate into large clumps that make it difficult for them to expand. Cell clumping

can both cause and lead to apoptosis or cell death.

Why is Cell Clumping a Problem?

When cells clump together, they restrict each other, lowering throughput and potentially jeopardizing downstream results.

Cell clumping also reduces target cell recovery and can interfere with labeling.

Certain cell analysis methods, such as flow cytometry, necessitate proper cell isolation.

Flow cytometry involves passing a large volume of cells through a machine in a rapid fluid stream.

The machine, known as a cytometer, detects differences in physical characteristics between different cells and labels them accordingly using fluorescent light. The cytometer will struggle to accurately measure the characteristics of cells that are clustered together as they pass through the tube. This can result in them being improperly sorted, which can have a negative impact on downstream results.

Causes of Cell Clumping Cells in culture may clump for a variety of reasons, including:  Over-digestion — Excessive use of certain enzymes used for tissue dissociation, such as trypsin, can cause cells to clump.  Environmental stress — Physical forces and repeated temperature changes can cause cell death, resulting in "sticky" DNA molecules that connect neighboring cells.  Tissue disaggregation — When preparing a single-cell suspension, using enzymatic, mechanical, or chemical dissociation strategies can rupture cells, resulting in clumping debris.  Overgrowth — When cells reach confluency or their maximum growth potential in their culture medium, they begin to lyse and release the debris.  Contamination — Certain bacterial and fungal pathogens cause cells to lyse; clumping is frequently a sign that a cell culture has been contaminated.

Pluriselect’s Innovative Technologies There is less residual DNA and debris floating around in the sample as a result of effectively sorting cells in a gentle manner that preserves cell health and physiology.

Pluriselect'sCell Separationproducts offer a quick and efficient cell separation method that yields a highly enriched sample of the target cells.

The three cell isolation technologies that we have developed with efficiency in mind are listed below: 1. Antibody supported cell separation: PluriSelect provides the pluriSpin negative isolation and depletion system, as well as the pluriBead positive isolation system.

2. Gravity Supported Separation: PluriSelect provides optimized density gradient media for the isolation of Leukocytes, Monocytes, Platelets, and PBMCs from older blood, as well as the most commonly used solution for PBMC isolation (density 1.077 g/ml).

3. Size Support Separation: Pluriselect Strainers are ideal for sample preparation.

Choose from meh sizes ranging from 1 um to 1000 um, sterile or non-sterile, PET or nylon fibers, back-flush capability, and small volumes up to 800 uL or in-line-strainer for your sample preparation.

For researchers needing an efficient tool for isolating specific cell types from various sample sources, we recommend trying Pluriselect.

Contact Us Website: https://uberstrainer.com/ Mail: contact.uberstrainer@gmail.com