Transnetyx Suite of Services Europe

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Welcome to the Responsible.

Reproducible.

Reasonable.

We leverage shallow shotgun, nextWhy you should outsource We’ve developed these services because: future of research generation sequencing totime. provide you your genotyping Serving research.

Saving with substrain-level insight Our MiniMUGA panel, developed by researchers at The University of North

WE CARE Hill, ABOUT WE CARE ABOUT Carolina at Chapel helps you determine the full background strain WE (or CARE Our automated genotyping service: We’ve built an entire suite of services to help Since we first launched automated genotyping in 2004, we DISCOVERY. ANIMALS. ABOUT YOU. substrain) of your mice, and avoid unwanted genetic variations caused by streamline your you canfaster, focus more on what have maintained ourresearch mission so to provide accurate, •easy Provides free custompart assay design •mixed Eliminates human Microbiome analysis in 3leads steps: backgrounds.

Thewith same tasks that Your research coulderror. have Animal care is an important matters most – research that tothan discovery. and more affordable methods to help support responsible, more 30,000 assays in stock across are necessary for good a• Saves significant impact on the of research.

Efficiency and proper you time. multiple Each supported by a dedicated team ready reproducible research.

It is this mission thatspecies. has the science can also seem like world.

Weservice believe inis you, care can promote the driven health only per sample, you’ll beofable to: •For Reduces cage $40 costs byUSD identifying to discovery. Each and wedeliver want to an help ensure your animals also protecting CLICK SHIP to exceptional experience. launch of the full suite of services you seewhile today.

• Offers dedicated, expertANALYZE supportroadblocks teams scientifically useful animals earlier. of these reproducible results. your results.

That’s our to serve yourwhy troubleshooting and assay services, designed half Confirm the Ensure withinyou in mind, can help services are created with the 3RsBackcross •These Accelerates your research. development needs. services help researchers reach publication faster and Enjoy clean, concise, and lower Send 2 fecal pellets per Place an order in your the time with background of lines reproducible you save time and effort.

(Replacement, Reduction, and interactive results in in the provided kit – free • Provides sample storage for six4months, QuickOrder account. ensure proper animal mouse care. Our commitment to animal welfare costs using speed imported from results.

Refinement) in mind. weeks or of less. no dry Reduction, icecollaborators. needed!avoiding the need to re-clip. congenics. and the 3Rs (Replacement, and Refinement)

laboratory animal science drives all our innovation. Our goal

is to help researchers likewe youcan accomplish Let us know how help.more with fewer

+1 888 321 2113 animals in less Our mission is totime. save you time and serve your Learn Start Get your testing how first to samples get 25 automated started for at only genotyping $40 USD samples help@transnetyx.com research. Tell us how we can help by contacting us at: transnetyx.com/microbiome. at transnetyx.com/monitoring. free at transnetyx.com/why-outsource.

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Fast, easy, accurate Reproducible, efficient automated genotyping research starts here Improve the efficiency and accuracy of your research.

For two decades, Transnetyx has served FASTthe research community EASY with automated ACCURATE genotyping that helps researchers save time and focus on results.

• Turnaround in 24 or 72 hours.

• Real-time PCR results, guaranteed.

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• Just clip, ship, and click.

• 99.97% accuracy.

• Average order time is less than 5 minutes.

• Duplicate sample processing with a housekeeping gene.

• Results accessed online.

transnetyx.com/why-outsource transnetyx.com/monitoring transnetyx.com/microbiome transnetyx.com help@transnetyx.com

Responsible.

Reproducible.

Reasonable.

We leverage shallow shotgun, nextWhy you should outsource We’ve developed these services because: generation sequencing totime. provide you your genotyping Serving research.

Saving with substrain-level insight Our MiniMUGA panel, developed by researchers at The University of North

WE CARE Hill, ABOUT WE CARE ABOUT Carolina at Chapel helps you determine the full background strain WE (or CARE Our automated service: Since we first launchedgenotyping automated genotyping in 2004, we DISCOVERY. ANIMALS. ABOUT YOU. substrain) of your mice, and avoid unwanted genetic variations caused by have maintained our mission to provide faster, more accurate, Provides free custompart assay design •mixed Eliminates human Microbiome analysis in 3 •easy steps: backgrounds.

Thewith same tasks that Your coulderror. have care is an important and research more affordable methods Animal to help support responsible, more than 30,000 assays in stock across are necessary for good a• Saves significant impact on the of research.

Efficiency and proper you time. multiple reproducible research.

It is thiscare mission thatspecies. has science can also seem like world.

We believe in you, can promote the driven health ofthe only perSHIP sample, you’ll be able to: •For Reduces cage $40 costs byUSD identifying to discovery. Each and we want to help your animals also protecting CLICK launch of the fullensure suite of services you seewhile today.

• Offers dedicated, expertANALYZE supportroadblocks teams scientifically useful animals earlier. of these reproducible results. your results.

That’s our to serve yourwhy troubleshooting and assay services, designed half Confirm the Ensure withinyou in mind, can help services are created with the 3RsBackcross •These Accelerates your research. development needs. services help researchers reach publication faster and Enjoy clean, concise, and lower Send 2 fecal pellets per Place an order in your the time with background of lines reproducible you save time and effort.

(Replacement, Reduction, and interactive results in in the provided kit – free • Provides sample storage for six4months, QuickOrder account. ensure proper animal mouse care. Our commitment to animal welfare costs using speed imported from results.

Refinement) in mind. weeks or of less. no dry Reduction, icecollaborators. needed!avoiding the need to re-clip. congenics. and the 3Rs (Replacement, and Refinement)

laboratory animal science drives all our innovation. Our goal

is to help researchers likewe youcan accomplish Let us know how help.more with fewer

+1 888 321 2113 animals in less Our mission is totime. save you time and serve your Learn Start Get your testing how first to samples get 25 automated started for at only genotyping $40 USD samples help@transnetyx.com research. Tell us how we can help by contacting us at: transnetyx.com/microbiome. at transnetyx.com/monitoring. free at transnetyx.com/why-outsource.

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5 7

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Fast, easy, accurate automated genotyping Improve the efficiency and accuracy of your research.

FAST • Turnaround in 24 or 72 hours.

• Real-time PCR results, guaranteed.

4

EASY

ACCURATE

• Just clip, ship, and click.

• 99.97% accuracy.

• Average order time is less than 5 minutes.

• Duplicate sample processing with a housekeeping gene.

• Results accessed online.

transnetyx.com/why-outsource transnetyx.com/monitoring transnetyx.com/microbiome help@transnetyx.com

Responsible.

Reproducible.

Reasonable.

We leverage shallow shotgun, nextWhy you should outsource We’ve developed these services because: generation sequencing to provide you your genotyping with substrain-level insight Our MiniMUGA panel, developed by researchers at The University of North

WE CARE Hill, ABOUT WE CARE ABOUT Carolina at Chapel helps you determine the full background strain WE (or CARE Our automated service: DISCOVERY. genotypingANIMALS. ABOUT YOU. substrain) of your mice, and avoid unwanted genetic variations caused by Provides free custompart assay design •mixed Eliminates human Microbiome analysis in 3 •easy steps: backgrounds.

Thewith same tasks that Your research coulderror. have Animal care is an important

moreEfficiency than 30,000 across are necessary for good a• Saves significant impact on the of research. andassays properin stock you time. multiple species. science can also seem like world. We believe in you, care can promote the health of • Reduces cage costs by identifying to discovery. Each and we want to help ensure your animals also protecting CLICK SHIP • Offerswhile dedicated, expertANALYZE supportroadblocks teams scientifically useful animals earlier. of these reproducible results. your results.

That’s our to serve yourwhy troubleshooting and assay services, designed half Confirm the Ensure withinyou in mind, can help services are created with the 3RsBackcross • Accelerates your research. development needs.

Enjoy clean,the concise, and lower Send 2 fecal pellets per Place an order in your time with background of lines reproducible you save time and effort.

(Replacement, Reduction, and interactive results in mouse in the provided kit – free sample • Provides storage for six4months, QuickOrder account. costs using speed imported from results.

Refinement) in mind. weeks or less. no dry icecollaborators. needed!avoiding the need to re-clip. congenics.

For only $40 USD per sample, you’ll be able to:

Let us know how we can help.

+1 888 321 2113 Our mission is to save you time and serve your Learn Start Get your testing how first to samples get 25 automated started for at only genotyping $40 USD samples help@transnetyx.com research. Tell us how we can help by contacting us at: transnetyx.com/microbiome. at transnetyx.com/monitoring. free at transnetyx.com/why-outsource.

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The right strain for your research, without a strain on your budget.

Mixed-background strains cause non-reproducible results and can waste your lab’s time and money. Our genetic monitoring service tests against 10,000 SNPs in 241 inbred strains – all for only $40 USD per sample. So you can protect your research and your funding.

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transnetyx.com/monitoring transnetyx.com/microbiome help@transnetyx.com

Responsible.

Reproducible.

Reasonable.

We leverage shallow shotgun, nextWe’ve developed these services because: generation sequencing to provide you with substrain-level insight Our MiniMUGA panel, developed by researchers at The University of North

CARE Hill, ABOUT WE CARE ABOUT CarolinaWE at Chapel helps you determine the full background strain WE (or CARE DISCOVERY. ANIMALS. ABOUT YOU. substrain) of your mice, and avoid unwanted genetic variations caused by Microbiome analysis in 3 easy steps: mixed backgrounds.

The same tasks that Your research could have Animal care is an important part

are necessary for good a significant impact on the of research. Efficiency and proper science can also seem like world. We believe in you, care can promote the health of roadblocks to discovery. Each and we want to help ensure your animals while also protecting CLICK SHIP ANALYZE of these services, designed reproducible results. your results. That’s why our half Confirm Ensure withinyou in mind, can help servicesthe are created with the 3RsBackcross Enjoy clean, concise, and lower Send 2 fecal pellets per Place an order in your the time with background of lines reproducible you save time and effort.

(Replacement, Reduction, and interactive costs results in 4 speed mouse in the provided kit – QuickOrder account. using imported from results.

Refinement) in mind. weeks or less. no dry icecollaborators. needed! congenics.

For only $40 USD per sample, you’ll be able to:

Let us know how we can help.

Our mission is to save you time and serve your Start how tosamples get forat only $40 USD research. Tell Learn us howtesting we can helpstarted by contacting us at:

transnetyx.com/microbiome. at transnetyx.com/monitoring.

+1 888 321 2113 help@transnetyx.com 7

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The changing microbiomes of your colonies can be a punch in the gut for the whole lab New evidence suggests the gut microbiome is the missing piece of the reproducibility puzzle, and that shifting gut microbiomes can significantly alter the phenotype of your mice.1,2,3

Caruso R, Ono M, Bunker ME, Núñez G, et al. Dynamic and Asymmetric Changes of the Microbial Communities after Cohousing in Laboratory Mice. Cell Reports. 2019;27(11):3401-3412.e3.

2 Franklin CL, Ericsson AC. Microbiota and reproducibility of rodent models. Lab Animal. 2017;46(4):114-122.

3 Hoy YE, Bik EM, Lawley TD, Holmes SP, et al. Variation in Taxonomic Composition of the Fecal Microbiota in an Inbred Mouse Strain across Individuals and Time. PLOS ONE. 2015;10(11):e0142825.

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transnetyx.com/microbiome help@transnetyx.com

We leverage shallow shotgun, nextWe’ve developed these services because: generation sequencing to provide you with substrain-level insight WE CARE ABOUT DISCOVERY.

WE CARE ABOUT ANIMALS.

WE CARE ABOUT YOU.

Microbiome analysis in 3 easy steps: Your research could have Animal care is an important part

The same tasks that are necessary for good of research. Efficiency and proper science can also seem like care can promote the health of roadblocks to discovery. Each your animals while also protecting SHIP ANALYZE of these services, designed your results. That’s why our with you in mind, can help services are created with the 3Rs Enjoy clean, concise, and Send 2 fecal pellets per you save time and effort.

(Replacement, Reduction, and interactive results in 4 mouse in the provided kit – Refinement) in mind. weeks or less. no dry ice needed!

a significant impact on the world. We believe in you, and we want to help ensure CLICK reproducible results.

Place an order in your QuickOrder account.

Let us know how we can help.

Our mission is to save you time and serve your to get at research. Tell Learn us howhow we can helpstarted by contacting us at:

transnetyx.com/microbiome.

+1 888 321 2113 help@transnetyx.com 9

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Streamlined research services from a team who cares Your research matters.

All over the world, researchers like you are making discoveries that improve the future of science and the lives of people all around the world.

Transnetyx is here to simplify that process for you.

©2021 YX Genomics. All rights reserved. Transnetyx is a registered trademark of YX Genomics.

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help@transnetyx.com

We’ve developed these services because: WE CARE ABOUT DISCOVERY. Your research could have a significant impact on the world. We believe in you, and we want to help ensure reproducible results.

WE CARE ABOUT ANIMALS. Animal care is an important part of research. Efficiency and proper care can promote the health of your animals while also protecting your results. That’s why our services are created with the 3Rs (Replacement, Reduction, and Refinement) in mind.

Let us know how we can help.

Our mission is to save you time and serve your research. Tell us how we can help by contacting us at:

WE CARE ABOUT YOU. The same tasks that are necessary for good science can also seem like roadblocks to discovery. Each of these services, designed with you in mind, can help you save time and effort.

+1 888 321 2113 help@transnetyx.com 11

©2021 YX Genomics. All rights reserved. Transnetyx is a registered trademark of YX Genomics.

TYX-350.000