The CROEV Newsletter • Issue 1 • May 2023

Dear readers,

The CROEV Newsletter is a space for students to express themselves and to learn about the art of writing. It is an honor to present the first issue, in which we included your achievements and your works of art. This is only the beggining of a long journey ahead...

We hope you enjoy our first ever edition!

DIRECTOR'S MESSAGE

"Esta es la primera edición de nuestro periódico escolar. Primero quiero felicitar a los estudiantes y su maestra por esta gran iniciativa. Esta será una herramienta que aprovecharemos para publicar todos nuestros logros y reseñar lo positivo en nuestra escuela. Aprovechemos esta oportunidad para darnos a conocer y seguir resaltando nuestros triunfos.

Jóvenes, gracias por ofrecer sus talentos para beneficio de nuestra comunidad escolar. Sigan demostrando que nuestra juventud está preparada para afrontar nuevos retos y utilizaremos este medio para darlos a conocer. Estoy muy orgulloso de ustedes y saben que cuentan con mi apoyo incondicional.

Mucho éxito en esta nueva encomienda y sigan adelante."

MEET THE CREW!

By: Prof. Fabiola Molina

EDITOR'S NOTE

A newsletter is a great way to keep the students, faculty, parents, and guardians abreast of what's happening in our school. It's an effective way of disseminating information and touching base with all the parties mentioned.

The value of the newsletter, however, lies in its creation. First, it needs an incredible team to make it happen and that's when our students come into play.

OUR TEAM IS COMPOSED OF STUDENTS FROM EVERY GRADE!

OUR LOGO

MEET T MEET THE CREW!

ALISHA PUBLISHER PATRICIA PUBLISHER

LUIS PUBLISHER

ADRIAN ACADEMIC EDITOR

KELLYANIS REPORTER

MARIA PAULA ACADEMIC EDITOR

ALONDRA REPORTER ANAIS REPORTER

NELSON PHOTOGRAPHER MIA REPORTER

DARIANA REPORTER

ANA ACADEMIC EDITOR

JULIANISSE CREATIVE EDITOR

ALERIS CREATIVE EDITOR

GABRIELA CREATIVE EDITOR

MIRNOSHKA ACADEMIC EDICTOR

MEET THE PROFESSORS!

PROF.NATALIA MARTÍNEZ TORRES

SCIENCE TEACHER FOR STUDENTS OF ALL GRADES, RESEARCH IS ONE OF HER PASSIONS

CO-ADVISORY TEACHER FOR THE CROEV NEWSLETTER

PROF. FABIOLA MOLINA SANTIAGO

ENGLISH TEACHER FOR 10TH GRADE AND 12TH GRADE, ADVANCED ENGLISH COURSE

ADVISORY TEACHER FOR THE CROEV NEWSLETTER

SHOOTFORTHEMOON Evenifyoumiss

YOU'LLLANDAMONGTHESTARS

SCIENCE

By: Adrian N. Escalera Rivera ACADEMICS

BY: ADRIÁN N. ESCALERA

During the years 2022-2023 several activities were carried out. Some of the professors who carried out these activities at our school are: Deborah Matías, Monica Anadón, Rocío Hernández, Yaharie Olivera, Natalia Martínez and Yesenia Torres. Among the activities are: climate change projects, environmental impacts, workshops, research, among others. Those courses that are being carried out in the year 20222023 are: Environmental Sciences, Organic Chemistry, Biology, Earth Sciences, Anatomy, Physics, Forensic Chemistry, Neurosciences, Scientific Research, Management and Waste, Botany and Chemistry.

D. Matías

Professor Deborah Matias from the Environmental Sciences, Organic Chemistry courses is currently participating in a climate change project It is entitled Climate Change Project: UPRM Youth Impact Its purpose is aimed at impacting high school youth by including courses on climate change in the curriculum of the Department of Education Three private schools and two public schools participate in the program, where CROEV is included Congratulations!

R. Hernández

Professor Rocío Hernández of the Biology, Neurosciences and Scientific Research course has carried out different activities during the year Some of them were: research for a science fair, workshops, visits to research centers, among others

During the year, Prof Hernández guided and served as a mentor for the research group, where the students showed interest in the areas of: cell biology, robotics, social sciences, among others She brought 9 projects to the 2023 Regional Science Fair and they all got great prizes Congratulations!

Y. Olivera

Professor Yaharie Olivera from the Biology, Earth Sciences and Anatomy courses has carried out different activities during the school year Among them are: neuroscience workshops at the Medical Sciences Campus and neuroscience workshops led by students from the Pontifical Catholic University of Puerto Rico

N. Martínez

Professor Natalia Martínez from the Management and Waste, Botany and Scientific Research courses stood out for carrying out different activities during the year Some of them were: Construction of the Butterfly Garden, Eco-Fashion Show, visits to recycling museums, community service activities, among others On April 30, 2023, the teacher carried out the Eco-Fashion Show activity, where her students modeled clothes designed from recycled materials The activity was a complete success, congratulations! Additionally, she will be going to ISEF 2023

M. Anadón

Professor Mónica Anadón of the Chemistry course has carried out and led different activities and organizations during the year Some of them are; SHPE club, school cooperative, aquatic robotics competitions, among others Together with teachers José Torres and Nilda Pérez, she will lead the aquatic robotics team to compete in Maryland from May 10 to 14 Congratulations!

Y. Torres

Professor Yesenia Torres, from the Physics and Forensic Chemistry courses, has participated in different activities during the year. One of them was the construction of projectiles with pvc material with his students from the Physics course In this project, her students designed projectiles (rocket launchers) After the design, they proceeded to build them and put them into practice on the field of our school Interesting activity, congratulations!

Women in STEM

Women in STEM have emerged as trailblazers, challenging traditional gender roles and breaking barriers in their respective fields. From scientists unraveling the mysteries of the universe to engineers designing groundbreaking infrastructure, women have made significant contributions that shape our modern world. In technology, female innovators have pioneered transformative digital advancements, empowering communities and fostering inclusivity. With unwavering determination and exceptional talent, women in STEM continue to inspire future generations, ushering in a future where gender equality and diverse perspectives thrive. All science educators of our school are women, and we dedicate these words to them. With their effort, dedication, and commitment they have achieved an excellent education at CROEV.

Timeline

Como requisito del curso: 'Metodología de la Investigación Científica', los estudiantes matriculados en el mismo, se dieron a la tarea de presentar sus propuestas en diferentes escenarios. La siguiente recopilación, resume las diferentes actividades y eventos en la que los #CientíficosTodoTerreno, demostraron sus habilidades y conocimientos científicos

Marzo 2'023

F E R I A C I E N T Í F I C A

R E G I O N A L 2 0 2 3

Estudiantes presentaron en la Feria Científica Regional, obteniendo distinciones y premiaciones en cada una de sus categorías

Grado 11:

Soaned Maldonado- 2do lugar en Ciencias Ambiéntales

Neythan Rodríguez - Premio especial en Ciencias Sociales

Grado 12:

Alenis Berlingeri- 2do lugar en Ingeniería Ambiental

Adrián Escalera- 1er lugar en Biología celular y Molecular

Christy Rosario- 1er lugar en Biomedical

Darielis Ortiz- 1er lugar en Bioinformática

Gustavo Báez- Mención honorífica y premio especial en Ciencias Sociales

Isabel Díaz- 2do lugar y premio especial en Ciencias

Sociales

Patricia Larrauri- 2do lugar en Medicina Translacional

Alisha Torres- 3er lugar en Medicina Translacional

Abril 2023

“ANNUAL SCIENCE AND TECHNOLOGY FAIR 2024”

Participación en evento auspiciado por el Ponceño Junior Doctors Las estudiantes Darielis Ortiz obtuvo el tercer lugar y a la estudiante Christy Rosado que obtuvo el primer lugar; en cada una de sus categorías correspondientes.

S I M P O S I O D E

I N V E S T I G A C I Ó N X X

P U C P R - P O N C E C A M P U S

Estudiantes matriculados en el curso de Investigación Científica, participando del Simposio XX de la PUCPR

MATH

By: Alisha Torres Fernández

BY: ADRIÁN N. ESCALERA

During the 2022-2023 academic year the Math Program reached many goals. Some of the many increible achievements were:

1- The celebration of Math Week, where students had the chance of learning and reinforcing their knowledge with fun interactive games

2- The Math Olympics, where our students won various recognitions

Regional Math Olympics

Maria P. Moreno- 2nd Place

Alvaro Moreno- 1st Place

Juan Santini- 1st Place

Kevin Cruz- 3rd Place

ACADEMICS

State Math Olympics

Alvaro Moreno- 1st Place

Juan Santini- 2nd Place

E N G L I S H

By: María Paula Moreno

This year, CROEV thrived, as usual, with their annual celebration of English Week: a week full of activities that promote the learning and appreciation of the English language Not only this, but it focuses on providing students with a fun and engaging way to improve their language skills During English Week, they can participate in a wide range of activities such as spelling bees, poetry, singing, and many more contests With their involvement, students enhance their language proficiency, critical thinking, and communication

Starting off the week with the “I Have a Voice” singing competition, a group of students had the chance to sing their favorite songs Through this platform the institution thanks and congratulates every single participant for their great effort and compromise towards extracurricular events such as this one We shall educate integrally and this is just one way to achieve that In this very competition, the student Daniela Collazo obtained third place at regionals Her effortless range captivates every audience she encounters But, more importantly, her passion for music is evident in every aspect of her performance Really a true artist Congratulations Daniela!

Moreover, the senior student Alisha Torres also participated in this week’s activities. She brightened the school’s spirit with an amazing Oratory performance. Her confidence, persuasive arguments, and powerful words have the ability to captivate crowds. Then again, congratulations Alisha!

Furthermore, Darielis Ortiz was part of a selected group of students who participated in the “Poetry Out Loud” finals. This contest consists of declaiming poetry in English. She succeeded in reaching the Top 6 students in such an event. CROEV is proud of you!

Additionally, the Spelling Bee was also held this week After rounds of words spelled, the winners of this competition were: Elika Figueroa, Patricia Larrauri, and Eylimar Santini Thank you for portraying the school's values through such a level of representation

In short, let us keep on promoting English Week the perfect way to motivate the students and educators alike, since it serves as an opportunity for the students to gain confidence in their abilities and develop a deeper appreciation for the language Meanwhile, teachers have a chance to interact and share with their students in many creative and meaningful ways: encouraging a love and passion for English, one profound enough to last a lifetime Happy English Week!

ESPAÑOL

Por: María Paula Moreno

Juana de Ibarbourou

El Centro Residencial de Oportunidades Educativas de Villalba no es la excepción cuando se trata de promover actividades que fomenten el desarrollo de nuestro idioma Español A lo largo del semestre se realizan eventos en los cuales los croevitas participan y dejan huellas en cada uno de ellos. De la mano de las profesoras de Español, Mildred Zayas y Merlesh Segarra, los estudiantes son capaces de desenvolverse en las diversas ramas del idioma tales como: poesía, oratoria, redacción, cartel y muchas más vías que sirven como impulso y crecimiento cultural Este año, la Semana de la Lengua, llevada a cabo por el Departamento de Educación de Puerto Rico, fue dedicada al gran poeta y compositor: Fortunato Vizcarrondo, quien es principalmente reconocido por su obra “¿Y tu abuela Dónde Está?” Al dedicarse dicha semana a tal artista, se estimula la investigación estudiantil por este personaje, la curiosidad y, sobre todo, el conocimiento

De la misma manera, la estudiante María Paula Moreno participó en el certamen de poesía. El mismo consistió en la elección de un tema entre los cuales se encontraban: el racismo, la negritud, la identidad y lo afroantillano María Paula eligió el tema del racismo para elaborar su poema: “Si todo fuera blanco ” Dicha composición obtuvo el segundo lugar a nivel regional. ¡Orgullosos de tu desempeño, Moreno!

Continuando, los estudiantes del duodécimo grado: Alisha Torres, Alondra Rivera y Adrián Escalera estuvieron representando a la institución en un certamen literario de la Fundación Walter McK Jones de Villalba, específicamente en la categoría de redacción de ensayos Por otro lado, la estudiante Jenieliz Moreno, también participó en la categoría de poesía en dicho concurso y, de la misma manera, compitió en el certamen de poesía de Seguros Múltiples Al igual, la estudiante Coralys Renta obtuvo el segundo lugar en el Certamen de Trova Regional. CROEV se enorgullece de tan abundantes talentos y les desea mucho éxito en su venidera vida universitaria!

Sigamos apoyando las actividades que estimulen el desarrollo de nuestro lenguaje. Cuidemos, cultivemos y amemos nuestro idioma: lengua llena de historia y cultura, con una riqueza léxica y gramatical que la hace única y distintiva. Una que invita a la reflexión, que nos permite expresar ideas complejas con una elegancia y precisión que pocas lenguas pueden igualar

En fin, el español: idioma que conmueve representación de las más grandes riquezas culturales y lingüísticas de nuestro mundo

“Lengua de toda mi raza, habla de plata y cristal, ardiente como una llama, viva cual un manantial.”

By: Ana P. Zambrana

ELECTIVES ART PORTS

In the different types of art classes that Professor Montalvo offers, some students participate in competitions with their work. This class can and has developed new talents in students with a lot of creativity An example of a contest at some participated in was Expression enge, which was sponsored by Walgreens. emester the student Yetsabel Soto won in coop contest and her work was exposed in the calendars of said cooperative.

sor Díaz gives different classes for the elective of al Education Some students didn’t know what they up for when they started his classes, but now they great at what they do. For example, in swimming María Paula Moreno and Emmanuel Maldonado know how to swim, now they both learned how to estyle, backstroke and floating In the P E class, was taught for a period, and that encouraged the -grade girls to want to play it and join the team. nks to that, the girls varsity soccer team is now pated in a tournament called “Liga Interescolar de Soccer del Sur”, where they placed 1st place

MUSIC

In the band and chorus class that Prof. Olivera offers there have been a lot of learning expeditions An example of this is when they were invited to the middle school Nélida Meléndez Meléndez in Orocovis to play in an event called “Modelando por el Autismo”. They will be doing a similar thing on May 18th in which they will be playing in the Honors Night of the school Herminio W. Santaella in Coamo On March 1st they went to the University of Puerto Rico in Ponce (UPRP) to an open rehearsal of the chorus of said school On another note, this teacher also prepared the “CROEV’S Got Talent” talent show which was a huge hit and also great practice for the student in her classes

ELECTIVES

By: Ana P. Zambrana

HISTORY

HEALTH STEM

This semester the Professor Meléndez offers the classes of “Gerosalud” and “Primeros Auxilios” in which there has been done different activities Some of said activities were the Health Week in February 13-17 where students made kites related to emotions, the CPR project (which is divided in hands only and advanced) that was with Doctor Lester Mercado who is a general medic, and many more Most students learned hoy to take someone ’ s pressure and pulse The ninth graders had a project called “Baby Egg” which lasted all of February This class took an expedition to the theater of Juana Diaz to see a play calles “Mis estudios, mi novio y yo ” where they explained the importance of self care. Let’s hope that the new semester brings exciting things for this class!

There are two professors that offer different classes for this elective This semester Prof Colón offers “Derecho y Sociedad” in which students have worked with archaeological records where they have done investigations of iconic monuments of Puerto Rico Said students have also done social paradigms where they have analyzed recent cases that affect the family life and its environment Some students that have surpassed the academic curve in this class have been Alejandra Tantao and Carelys Sanchez because some of their investigations have raised awareness about the effects that are achieved through the application of human rights Prof Dávila also gives the class of this elective. She offers Civics with Ethics. These electives that she offers are selected based on the need that students have to learn about some subjects that are understood to be important or meaningful in their future lives. It is important when students come prepared for this class because it means that they are learning about things that they might use in their future. Two students who do this for said class are Adrian Escalera and Gabriel Nieves, who show an interest beyond the class and come prepared with information when a new topic is presented. A very special project that that Prof. Dávila likes to give is “Cómo manejar mi dinero en una economía difícil” where the students are given an adult life and earnings, given that they had to manage their money by making a budget, look for a home and cars. This is a great way of preparing these students for managing money for years to come.

Some of the most important projects that were made in Prof Torres classes represent us as a school around the island and the United States In December, the students made 3rd place in the Robot Maker competition by presenting their smart robot with a prize of $500 This semester the Watasumi robotics club will represent Puerto Rico in Maryland on May 10th. On another note, the student Alenis Berlingeri got second place with a project about the ocean ’ s acidification. This course offers a mix of math and science to apply it in our everyday use of technology

MEET YOUR PROFESSORS

WHAT WAS YOUR FAVORITE CLASS IN HIGH SCHOOL?

"MY FAVORITE CLASS WAS ALWAYS HISTORY"

WHICH TEACHER IMPACTED YOU THE MOST AND WHY?

HE WAS IMPACTED BY A VERY DIFFICULT MATH PROFESSOR IN 8TH GRADE WHO HE LATER IN LIFE MET AGAIN AND REALIZED HOW GOOD HE ACTUALLY WAS.

WHY DID YOU DECIDE TO STUDY MATH?

HE STUDIED EDUCATION BUT HE NEVER IMAGINED TO BE A MATH PROFESSOR, LIFE JUST TOOK HIM THERE.

WHAT INSPIRED YOU TO BE A TEACHER? HIS GRANDPARENTS, BECAUSE THEY WERE ALSO TEACHERS.

WHAT DO YOU LIKE THE MOST ABOUT CROEV?

HOW ORGANIZED IT IS AND HOW GOOD HIS CO-WORKERS ARE.

IF YOU WEREN’T A TEACHER WHAT WOULD YOU BE? SOME KIND OF ARTIST OR DJ.

WHAT DID YOU STUDY AND WHERE? HE STUDIED EDUCATION WITH A CONCENTRATION IN MATH AT THE PONTIFICAL CATHOLIC UNIVERSITY OF PONCE.

FUN FACT!

Francisco was a "bad student", especially in math and he ended up teaching it and liking it when he finally understood it

SCHOOL CLUBS

By: Aleris Maldonado

CROEV has a variety of students, all with different interests. Many of the students may be interested in different activities, such as cooking, reading, learning about future careers, etc. Because of this, there are a variety of clubs students can choose from. For cooking centered clubs, look no further than “La Cooperativa Juvenil” and “Café Arte”. The National Honor Society and SHPE offer information regarding careers and potential interests students may have. The Library club and the Interdisciplinary Team offer different activities, such as promoting the development of students’ literary expression, or offering seminars discussing topics that affect the community’s livelihood as a whole. Below is a brief summary for each club, explaining what they are based on and what to expect from them.

The Society of Hispanic Professional Engineers (SHPE), led by the student Adrian Escalera, has the purpose of creating a society of engineers to serve as role models for latin youth This organization promotes career development and professional advancements in Hispanic engineers and students Students can expect an environment where they can develop intellectually and professionally This is by figuring out where their interests lie and, whether or not they are actually interested in engineering. CROEV is a part of the RUM chapter, where students have taken part in different activities, such as the Kick-Off, where members of the club can expect seminars about different majors offered in the University of Puerto Rico, Recinto de Mayagüez

The National Honor Society (NHS), led by the student Alan Torres is a student organization that recognizes scholarships, student achievements, leadership, service, and character The club provides information regarding different careers, scholarship opportunities, and it helps students gain hours that are necessary for graduation The club was recognized internationally in January 2023, with the chapter initiation The council is 100% managed by students Moreover, the students have been responsible for most of the activities. Such as Forest Cleaning and Beach Cleaning All of these taught the students how to take care of the environment, in addition to giving them a total of seven green contact hours NHS provides the graduates with pendant stoles and certificates

SHPE NHS LIBRARY CLUB

Next up, the Library Club This club is one characterized by how active it is within the community. They are constantly offering new activities for members and to anyone that wishes to join Their recent activities include a Book Character Night, which involves dressing up as a book character and providing a background for said character and the book they come from, a Poetry Night, where students are welcome to share original pieces of poetry or to simply bring a poem they like In addition, the club has also done a Night of Legends, where students share a legend, whether it is one from their hometown or an urban legend, and share it with everybody Lastly, their most recent activity involves the Guest Author José Enrique Laboy In this activity students had the chance to learn the route needed to take in order to write a book. Some values club members have are the following, consistency, responsibility Lastly, students are required to do two hours per week work shifts, which will help them gain community service hours that are required for graduation.

COOPERATIVA

“La Cooperativa Juvenil” is a club where students can learn how to be in a working environment where they deal with money, customers, and food preparation In addition, the club is 100% student run The only part that isn’t run by students is the actual money management, which is deposited in the bank sponsoring this club The menu and food prepared are all in an affordable range for every student to enjoy Members of the club are required to work at least one hour a week These hours can be used for community service that is essential for graduation At graduation members are given a check using the percentage of the hours they worked and the money they spent

INTERDISCIPLINARY TEAM

The Interdisciplinary Team’s purpose is to unite social work, psychology, and nursing under the same objective, which is the student and any need they may have They are the people behind the different seminars regarding mental health, bullying, public safety, etc , which are given to the students to promote a healthy environment in their lives. Their most recent activity includes an array of different kiosks with the same purpose, generating donations for the organization “Relevo por la Vida” This organization focuses on helping cancer patients of all ages, that may be through helping ease the treatment costs, providing patients with psychological help, temporary housing for patients whose treatments are too far away from their homes, etc On may 20th, the school will be hosting the official relay for this year Students are welcome to come and join along their family members and enjoy the afternoon

EXPRESARTE

“Café Arte” is a branch from the club “Expresarte” This club has the purpose to help manage the cost of art materials and different activities students may have. In addition, it also helps raise funds for extracurricular activities This club promotes students to develop decision making skills, teamwork, money management, and independence The work environment is relaxing and helps students destress after a long school day with a nice coffee. This club provides students with community service hours depending on how long each student's work shifts are

CHAMPIONS WATATSUMI

EFFECTOFOSMOTICSTRESSONTHEEXPRESSION OFTHEBETAAMYLOID1-42GENEOFALZHEIMER’S DISEASEINTRANSGENICC.ELEGANSMODEL

Alzheimer'sdisease(AD)isdemarcatedasaprogressivecognitive neurodegenerativedsorderresultingfromtheirrationalnumberofpeople affectedworldwide(Bonilla-Santosetal2021)

Ithasbeenshownthatamongthemostnotableeffectsproducedby Alzhemersarememorylossinadultsspatialdisorientationapraxia attentionproblemsprocessingspeedexecutivefunctioningamongothers (ClevelandCnic,2022)

•Osmoticstressscharacterizedbybeingrelatedtoosmosis,whichisthe movementofthesolutedowntheconcentrationgradient(Giletal,2017)

•DuetothelackofeffectivetherapiestotreatAlzheimer'sNationalHealth OrganizatonsastheWorldHealthOrganization(2023),hasclassifiedthis diseaseasoneofthemostcomplexdiseases

•Currently,about50millionpeoplesufferfromtheeffectsofthisdisease, anditispredictedthatin25years,thisnumberwillincreaseto100million (WHO2023)

•BetaAmyoid(Aβ)1-42,isthegeneinvolvedintheprocessbeingstudied, whichalsoplaysaroleasabiomarkerofthediseaseitselfItshoudbe notedthatthenematodeusedwaspreviouslygenetcallymodifiedandthat alsothisisahumangene

IMAGE1

Ceegans

lfecycleat22C

NoteSmonFraserUniversty(2018,anuary12)WhatisCelegans? https://wwwsfuca/bioogy/faculty/hutter/hutterab/research/Celeganshtml

PROBLEM

Caenorhabditiselegans

DoesosmoticstressamplifytheexpressionoftheAlzheimersdisease Aβ1-42geneintransgenic?

HYPOTHESIS

Caenorhabditiselegans

β

IfosmoticstressisinducedinwiththeAlzheimersdiseaseA142genethenitsexpressionwllbeamplified

METHODOLOGY

TABLE1*

BetaAmylod(Aβ)1-42actvated2120)andnactivated(2122)geneofAzhemersdsease

FROM CROEV TO THE WORLD

ADRIAN ESCALERA AT ISEF 2023

NoteUsingafuorescencemcroscopetwasviabetomeasurethefuorescence intensty ineachoftheCeegansItshoudbenotedthattheareaoftheanalysiswas withinthesamerangepreventingtheareafactortoaffecttheresultsThetableshows thevauesobtainednthefourgroups

DATAANALYSIS

FIGURE1*

Independent-sampet-TestcaculatedcomparingmeansoftheexpressonoftheAβ1–42geneofAzhemersdsease

Note:

ThemagesweretakenbythefnaistDiagramwasmadebyfinalist

1InductionofNaCl(SodiumChloride)

Usingaconcentrationof250millimolesofNaC(SodumChloride) osmoticstresswasinducedineachnematodeForthis,theagarwas preparedbeforehandwithaconcentrationof250millimoarAfter this the nematodes grew and developed while under direct exposuretoosmoticstress

2Synchronization

Inordertohavefavoraberesultswhenanalyzingeachworm,only thefirstlarvalstageofCeleganswasselectedfromeachpetridish Thismadeiteasierfromallofthemtobeinthesamelarvalstage and thusagewouldnothavebeenafactorinfluencingtheresultsFor thisseveralwasheswereperformedwiththeM9BufferreagentThe equipmentusedwasthecentrifuge,whichwasusedat1,500rpmfor 2minutesatatemperatureof20°C

3Geneactivation

●

●

TheAβ1-42geneofAlzheimersdiseasewasactivatedbyatering thetemperatureThiswashandledandbroughtto25⁰C

4Fluorescenceexpression

Throughafuorescentmicroscope theexpressionofthegene couldbeanalyzedHavingtheintensitydatathesewereanalyzed graphicallyandstatistically

NoteSignificantdfferencebetweenthemeanofthetwogroupsp<05)wasfoundThe meanofthecontrolgroupCL2122wassignificantyower(M 330801p 000520than themeanoftheexpermentalgroupCL2120M=424366p=001877)

FIGURE2*

MeansoftheexpressionoftheAβ one-wayANOVA

1–42geneofAlzheimersdiseasecomparedusing

THE REGENERON INTERNATIONAL SCIENCE AND ENGINEERING FAIR (REGENERON ISEF) IS THE WORLD'S LARGEST INTERNATIONAL PRE-COLLEGE SCIENCE COMPETITION EACH YEAR, ISEF BRINGS OVER 1,800 HIGH SCHOOL STUDENTS FROM 75 COUNTRIES TO EXHIBIT AND DEMONSTRATE INGENUITY AND INNOVATION THROUGH SCIENCE PROJECTS OF THEIR OWN DESIGN.

NoteAsignfcantdfferencewasfound(f948p<05)TukeysHSDwasusedto determinethenatureofthedifferencesbetweentheexpressonofthegene Ths analysisreveaedtherewasasignificantdifferencebetweenthefuorescenceofthe controlCL2122(wthoutNaClandtheexperimentagroupCL2122-CL2120(wthNaC (p<001 Howevertherewerenosgnfcantdifferencesbetweenthecontrolgroupand experimentalgroupwithNaC

FIGURE3

NoteAlthoughtheosmolaritywasclearlymanifestedtheAβ1-42genedidnothavea significantchangeduetotheeffectsofosmotcstressHoweverwhattheresearch showssthatautofluorescencelevelsincreaseasanindicatorofoxdatvestressn CelegansmodelsthatexpresstheAzheimersgene

*NoteAlthefiguresweremadebythefnalist

Duringtheexperimentationprocesstheeffectsofosmoticstressonthe expressionoftheAβ1-42geneinCelegansnematodeswere evaluated TheStatisticalProductsandServcesSolutionPlatform(SPSS) wasusedtoperformboth at-testandANOVAanalysis Thesample consisted of a control group and an experimental group with 5 nematodesineach,foratotalof20sampesOncetheexperimentation wascarriedout itwasobservedthat althoughosmoticstresswas inducedbyaddingaconcentrationof250millimoarNaClthiseffectdd notamplifytheexpressionoftheA1-42geneofAlzheimer'sdisease

β

CONCLUSION

β

Themainpurposeofthis nvestigationwastoevauatetheeffectsof osmoticstressontheexpressionoftheA1-42geneofAlzheimers disease Forthis astatisticalanalysisofthedatacollectedinthe expermentationprocesswascarriedoutWithSPSSstatisticalanalysis andchartsweredevelopedMeaningCelegansexposedtoNaClinfact sufferfromosmoticstressHowever,whenanalyzngthefluorescenceof thecontrolandexperimentagroupitwaspossibletoconcludethat osmoticstressdoesnotamplifytheexpressionoftheAβ1-42geneof Alzheimer'sdisease,thusacceptingournullhypothessThismeansthat increasingtheosmolarityto250mMintheagarhasnoeffecton AlzheimersgeneexpressionFurthermoreitappearstobethecasethat thepresenceoftheGreenFluorescentProtein(GFP)tag(nematodesautofluorescence)increasesthesusceptibiltytoosmolarityanditsbrghtness orthatthepresenceofGFPismaskedbythesensitvityof autofluorescenceLikewiseitisimportanttoruleoutriskfactorsthatmay bepossiblecausesforthedevelopmentofdseasesasprogressiveasAD sinceopeningnewlinesofresearchbyruingoutothersgivesusthe opportunitytomitigatetherepercussionsofdiseasesthatthreaten society

PROJECTIONS

CarryoutasimilarinvestigationcausingacuteorchronicstressinC elegans

Includefutureexperimentswith20degreeswithoutactivatingthegenein ordertoreachstrongconclusionsand/oraddfilterstothefluorescence microscopetoavoidpossblemaskngofGFPplusthegene

Increasngoursampleinordertoprovethatineffectbyinducing osmoticstressthereisnoincreaseintheexpressonoftheAβ1-42geneof Alzheimer'sdisease

REFERENCES

AlzhemersDisease:Causes,Symptoms,Treatment&Stages(2022,December12,)ClevelandCinichttps://mycleveandcinicorg/health/diseases/9164-alzheimers-dsease

Bonilla-SantosJZea-RomeroEYGonzález-HernándezA&Cala-MartínezD(2021)Marcadorescogntivosboógicosanatómicosyconductualesdeldeteriorocogntvoeveylaenfermedadde

AlzhemerUnarevisiónsistemátcaRevistaEcuatorianaDeNeurología30(2)57–67https://doorg/1046997/revecuatneurol30200057

IgualGlCJariusMVonKriesJP&RohfingA(2017)NeuronalChemosensationandOsmoticStressResponseConvergeintheRegulationofaqp-8nCeegansFrontersinPhysoogy8380 https://doiorg/103389/fphys201700380

SmonFraserUniversity(2018,January12,)WhatsCelegans?https://wwwsfuca/boogy/faculty/hutter/hutterab/research/Celeganshtml WordHealthOrganzation(2023)Dementiahttps//wwwwhoint/news-room/fact-sheets/detai/dementia

CROEV CROEV VARSITY VARSITY

FIRST PLACE WINNERS

"LIGA INTER ESCOLAR DE SOCCER DEL SUR"

78 PUEBLOS 1 ESCUELA

By: Alondra Sofia Rivera & Kellyanis A. Birriel

Every month we will choose a city that is represented in CROEV by our students.

OUR VILLALBEÑOS

Fotógrafo Nelson Clavel

Villalba is a town in southern and central Puerto Rico with a rich and diverse culture Since its foundation in 1917, it has become an important educational, commercial, and tourist center. The culture of Villalba has influences from African, Taíno, and Spanish heritage Residents are known for their love of music, dance, and food and celebrate numerous parties and festivals throughout the year The Villalba Residential School of Educational Opportunities (CROEV) is located in the town. It is a prominent educational institution in Villalba and offers a wide variety of academic and extracurricular programs for students from all over the island Villalba is also known for its innovative and entrepreneurial spirit, with many successful local businesses and an increase in investment in technology and entrepreneurship in recent years The municipal government has launched small business support programs to promote local economic growth and employment The town of Villalba has many small and medium-sized businesses dedicated to manufacturing, commerce, and tourism. Residents are friendly, welcoming, and always willing to share their culture and heritage In summary, Villalba is a vibrant and pleasant community with a rich culture and education

COMMUNITY COMMUNITY SERVICE

By: Prof. N. Martínez

FEBRUARY 2023: CLEANING PLAZA DE COAMO, PR

Students enrolled in the course: Solid Waste Management (Prof N Martinez), attended the parties prior to the San Blas Marathon for the receipt of single-use plastics, paper and cardboard. This, with the initiative to reduce the amount of garbage in public spaces and help in its collection and proper separation

APRIL 2023 BEACH CLEANING WITH JETBLUE

Students who are members of the 'National Honor Society', together with Prof. Y. Dávila, joined JetBlue's efforts for a beach cleanup in the Municipality of Juana Díaz, PR. This, as an initiative in accordance with the Month of Planet Earth.

SENIOR SENIOR SENIOR SPOT SPOT SPOT ZEPHYR

The Zephyr 2023 class was quite a memorable one. They went through ups and downs like any class and yet they made their year memorable not only for them but for the whole school. The theme of the class was the roaring 20's where their senior entrance was a red carpet from celebrities of that era and every senior played one of those celebrities.

In their many achievements there was kiosks, winning funds for the class in TV programs and much more.

By: Patricia Larrauri

The class consists of many brilliant, artistic and overall aspiring minds that will impact the world for the better.

BY ANAIS RODRÍGUEZ

##SIDEHUSTLES SIDEHUSTLES

Kellyanis A. Birriel

Kelly’s Ice Cream (2022) & Ricas Tetitas (2019)

"I wanted to change the concept of my other business model and I decided to create a new program where I could catch the attention of more children. It’s a family business and its purpose is to promote small businesses in our island."

Instagram: @kellys_icecream

Eilys Cintrón

E.M’s Custom Keychains (2021)

Eilys’ small business consists of the personalization of keychains made out of resin. Each one of them are custom made to fit the client’s taste and the occasion. She started making keychains as a hobby. She then got inspired by the artisans she saw in different activities and started selling them.

Instagram: @e.mcustomkc

Leehandra L. Vasquez

Lee’s small business consists of the design of many different creative things. They include jewelry, pieces of art made out of ceramic, crochet, manicure and pedicure. Their main motivation for the development of this small business was their mom, who told them to start selling all the things they made as a hobby.

Instagram: @inspirewierdness

OUR STUDENTS ARE TALENTED ENTERPRENUERS AND THIS SPACE IS CENTERED ON SUPPORTING AND RECOGNIZING THEIR TALENTS.

CROEV SURVIVAL GUIDE

ADVICE FROM AN EX-ALUMNI OF CROEV FOR YOU, IF THEY DID IT YOU CAN DO IT TOO

ANGELYS RIVERA

ENTRA A LA UNIVERSIDAD TENIENDO METAS, PERO MANTÉN TU MENTE ABIERTA A OTRAS POSIBILIDADES, CARRERAS O PUERTAS QUE SE ABRAN EN EL CAMINO

ENTRA A TANTAS ORGANIZACIONES ESTUDIANTILES COMO PUEDAS HAZ CONEXIONES EN ELLAS, TANTO DE AMAZING FRIENDSHIPS COMO DE MENTORES QUE TE PUEDEN ABRIR UNA PUERTA EN EL FUTURO

NO HAY APURO CADA ESTUDIANTE TIENE SU PROCESO, TRAYECTORIA Y TIEMPO. DENTRO DE LAS METAS QUE TENGAS, TAMBIÉN DEBES TENER PRIORIDADES.

ORGANIZA TUS SEMESTRES DEL BACHILLERATO. BUSCA EL CURRÍCULUM DE TU BACHILLERATO EN EL WEBSITE DE TU UNIVERSIDAD Y LUEGO PREPARA UN EXCEL SHEET DE LO QUE SERÁN TUS SEMESTRES DE BACHILLERATO BASADO EN ESE CURRÍCULUM

CROEV GRAD 2021

APROVECHA CADA CLASE QUE TOMES PORQUE SERÁN TU PREPARAMIENTO PARA TU VIDA COMO PROFESIONAL, SO DON’T TAKE THEM FOR GRANTED.

SI ESTÁS EN EL SISTEMA UPR: DEFIENDE TU UNIVERSIDAD MUCHOS TE DIRÁN QUE “NO QUIEREN PERDER SU SEMESTRE” O “ESTÁS AHÍ PARA ESTUDIAR NADA MÁS” SIN EMBARGO, EL PODER DEL PUEBLO ESTÁ EN SU UNIVERSIDAD Y NO PUEDES PERMITIR QUE LA SIGAN HACIENDO MENOS ACCESIBLE CADA VEZ

PARA MIS CROEVITAS QUE ENTRAN A UN BACHILLERATO EN CIENCIAS: GUARDEN ESA LIBRETA DE QUÍMICA, BIOLOGÍA, PRECA Y CÁLCULO DE CROEV PORQUE LES DARÁN USO EN SU 1ER SEMESTRE DE BACHILLERATO Y MAYBE 2DO SEMESTRE TAMBIÉN.

NO TE FRUSTRES. ES NORMAL FALLAR. ES NORMAL COLGARSE EN UNA CLASE (IMPORTANTE: LA TIENES QUE REPETIR). ES NORMAL DARSE DE BAJA DE UNA CLASE. ES NORMAL CAMBIARSE DE CONCENTRACIÓN. ES NORMAL SENTIRTE SÓLO(X). LO IMPORTANTE ES QUE SEPAS QUE TODO PASARÁ, ES CUESTIÓN DE TIEMPO.

CONSEJO PARA UNA VIDA DE COLLEGE STUDENT STRESSFREE: NO DEJES TODO PARA LO ÚLTIMO. BUSCA MANERAS DE EVITAR LA PROCRASTINACIÓN (TE LO DICE ALGUIEN QUE PROCRASTINA BASTANTE SO POR EXPERIENCIA, NO LO RECOMIENDO).

By: Sofia I. Torres

Croevita CroevitaInventions Inventions

IN THIS SPACE WE SHOWCASE THE ART OF OUR STUDENTS.

By: Nayedi Pagán (10)

By:YetsabelSoto(10)

ART CREATED BY MIGUELÁNGEL SERRANO PÉREZ (12TH GRADE)

Beacon of Light

C R O E V I T A ' S I N V E N T I O N S

Lo que fue

Tonight was dark

Like a closed room with the lights off

A starless night

Like the feeling of a hopeless lover

A blank canvas

Like an artist without a muse

Then you came;

Big and Bright

Like a child’s smile

Lighting the dark

Like a spark in the sky

I beseech you moon

Shine every night

So that way

I may never feel alone

By: Genesis Ailiaris Perez

Me duele saber que te perdere

Que jamas en mis brazos te tendre

Acostumbrarme a tu ausencia sera mi nuevo deber

Recordar tu mirar sera un placer

Tus caricias atesorare

Aunque lejos estes

By: “Esmeralda”

There’s no racism but

By: Alisha N. Torres Fernández

Often heard in Puerto Rico is, there is no racism but…

There is no racism but, no governor has been black

There is no racism but, our people can barely accept to write black on the census,

There is no racism but, we shouldnt marry black people so we don't ruin the race,

There is no racism but, a respected black politician is called a monkey, There is no racism but, I’m ‘negrita pero bonita’, There is no racism but, braids make me rasta, There is no racism but, I should stay out of the sun so I don't get so dark, There is no racism but, wearing my hair curly means ‘I'm in touch with the community’,

There is no racism but, relaxers have been saturated in my hair since before I could multiply,

We keep hearing that there is no racism, Yet every black person I know has experienced some type of attack, These were only some of my memories, but if I asked other people i’d be here for hours,

So if this is not called racism, then what is it called?

Save The Date

By: Gabriela I. Matos Rivera

ALL I SEE IS RED (SHORT STORY)

BY FABIOLA MOLINA SANTIAGO

Fire, smoke, gun shots… at least that is what I thought I heard as I walked through the sea of people surrounding me as if I were a precious stone. All I could of was my red dress with the satin bow that my grandmother made for me when I was 4. The sparkles of my black, small shoes were now covered in mud and despair. I was desperately looking for the two people who matter the most. I was ripped out of their arms like an angel being thrown out of heaven. I just wanted to hear my dad say, don’t miss me that much, as he left for work. But now I was confused, the surrounding people did not help. At my right, I could see a woman crying, with her torn out black fur coat, her heavily used gray shoes that looked as if they were about to break. She looked at me with eyes that could scream for help, she cried as if she knew me or I reminded her of someone. To my left, I had a big man with a toy gun, or so I thought. He had badges with a symbol I simply could not recognize, but I knew my mom would help me, that’s why I am trying to find her. The man looked at me with a cold stare, an almost sympathetic glare, and an expression I could not understand. I kept walking. I just wanted to find my parents. But then, a loud sound stopped me as it rumbled through the path I was taking, people cried around me even more, rocks moved as if they had life of their own, fire consumed once again my surroundings and smoke that could last for days did not let me breathe. I had nowhere to go. The crowd got bigger, the screams got louder, the gunshots that I thought where from a toy were now hurting the people around me I must admit I ran as fast as a jaguar looking for its prey. I was not fast enough, and as I passed the burning flames, I saw my mom and my dad screaming for help. Help I could not give them, as much as I wanted to. What could a small 7-year-old do? They were on the floor, and I tried to get as close to them as I could. I guess it wasn’t enough. Everything was turning into small black and white dots and as I looked down, I saw my evanescent coat getting redder. I was scared, as I screamed for help I knew no one would stop. The weather got cold, my feet got tired, I laid on the ground as a leaf in spring just waiting to be picked up but no one came. I saw more red, that lovely color, and as my eyes closed I said one last time…

“Mom? Dad? Don’t miss me that much”

STUDENTCOUNCIL STUDENTCOUNCIL

WORK BY LUIS LUNA

"EL CONSEJO ESTUDIANTIL REPRESENTA LA VOLUNTAD DE LOS ESTUDIANTES Y SE CREÓ CON EL PROPÓSITO DE COMUNICAR SITUACIONES Y EXPRESAR LAS INQUIETUDES DEL ESTUDIANTADO CROEVITA. QUEREMOS MEJORAR LA COMUNICACIÓN ENTRE ESTUDIANTES Y ADMINISTRACIÓN, CREANDO UN AMBIENTE DE CONVIVENCIA SANO PARA

MEJORAR LA EXPERIENCIA EN CROEV. PLANEAMOS ACTUALIZAR EL REGLAMENTO ESCOLAR, Y CREAR UN MANUAL DE CONVIVENCIA ESTUDIANTIL. ESTE DETALLARÁ LOS DERECHOS Y RESPONSABILIDADES TANTO DE ESTUDIANTES COMO CUIDADORES, PROCTORS Y GUARDIAS PARA GARANTIZAR UNA

MEJOR CONVIVENCIA Y MENOS DISCREPANCIAS. SIENTO QUE

UN BUEN MOTIVADOR ES EL MENSAJE QUE SIEMPRE ESCUCHAMOS:

CROEV SOMOS TODOS, Y SI ERES CROEV, QUE SE TE NOTE.

LA RESPONSABILIDAD DE MANTENER LA ESCUELA EN LA POSICIÓN EN LA QUE ESTÁ DEPENDE DE NOSOTROS.

CUALQUIER INQUIETUD, PROBLEMA O SITUACIÓN QUE NO TE HAGA SENTIR CÓMODO LA PUEDES COMUNICAR CONMIGO O CUALQUIER OTRO MIEMBRO DEL CONSEJO. PIENSA EN CÓMO

MEJORAR TU EXPERIENCIA Y LA EXPERIENCIA DE LOS FUTUROS CROEVITAS, Y NO TE CONFORMES CON LOS PROBLEMAS. ¡ESTAMOS AQUÍ PARA USTEDES!"

MESSAGE BY NEYTHAN RODRÍGUEZ

IIT'SYOUR IT'SYOUR T'SYOUR TURNTO TURNTO TURNTO DECIDE... DECIDE... DECIDE...

WHICHSHOULDBETHENEXT CITYFOR"78PUEBLOS,UNA ESCUELA"?

WHICH PROFESSOR DO YOU WANT US TO INTERVIEW NEXT?

WHATSHOULDWEADD FORTHENEXTEDITION?

SUMMER CHECKPOINT

BY PATRICIA LARRAURI & ALONDRA RIVERA

Park with high-thrill attractions like a 2.5-km. zip line & ropes courses through the jungle.

Located in Orocovis, Puerto Rico

The MAC is the only institution in the island expressly dedicated to the study, collection, preservation, exhibition, and promotion of art produced since the mid-20th century in Puerto Rico, the Caribbean, Latin America, and its diasporas Located in San Juan, Puerto Rico

Buyé Beach located in Cabo Rojo, Puerto Rico. It has cristal clear and calm waters. Also, it has strips of white sand, as well as tranquility and harmony that gives us the chance to relax after a long school year

TOP 3 PLACES TO VISIT DURING THE SUMMER

EXPLORE•LEARN•RELAX

BUYE BEACH

TORO VERDE ADVENTURE PARK

MUSEO DE ARTE DE PUERTO RICO

SPECIAL THANKS TO EVERY STUDENT THAT MADE THIS NEWSLETTER POSSIBLE. SHOUT OUT TO ALISHA TORRES AND PATRICIA LARRAURI FOR MOTIVATING ME (PROF. MOLINA) TO ACTUALLY MAKE THIS A REALITY. TO EVERY PROFESSOR THAT COLLABORATED, THANK YOU. SPECIALLY, THANKS TO PROF. N. MARTINEZ FOR ALL OF HER HELP. HOPEFULLY, YOU WILL SEE MUCH MORE OF OUR NEWSLETTER.

IF YOU WISH TO JOIN OUR TEAM, PLEASE CONTACT ME AT: FMOLINA@CROEVSCHOOL.ORG