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ThestudyrevealedthatcomparedtoculturethesensitivityofUltrawas5%higherthanthatofXpertMTB/RIF(%vs%)butTheXpert®MTB/RIFAssay, performedontheGeneXpert®InstrumentSystems,isaqualitative,nestedreal-timepolymerasechainreaction(PCR)invitrodiagnostictestforthedetectionof MycobacteriumtuberculosiscomplexDNAinrawsputumorconcentratedsputumsedimentpreparedfrominducedorexpectoratedsputumXpertMTB/RIF UltraprovidesfastandhighlyaccuratedetectionofMycobacteriumTuberculosis(MTB)andRifampicin(RIF)resistancesimultaneouslyfrompatientsamples; Highersensitivity,especiallyinsmear-negativeTBcasesandamongHIV-infectedpatients3;Simplethree-stepprocesswithminimalhands-ontimeandresultsin ~mindirectInsertcartridgeandstarttestsampleintocartridgeXpert®MTB/RIFUltraisasemi-quantitative,nestedreal-timePCRinvitrodiagnostictestforthe detectionofMycobacteriumtuberculosis(MTB)complexDNAandrifampin-resistanceassociatedmutationsoftherpoBgeneTheXpertMTB/RIFassayisa newtestthatisrevolutionizingtuberculosis(TB)controlbycontributingtotherapiddiagnosisofTBdiseaseanddrugresistance.theobjectiveofthestudywasto Ultra,XpertMTB/RIFandculture.However,ithasreasedsensitivityinsmear-negativesputum,andfalseidentificationofRIF-Roccasionallyoccurs.Thetest simultaneouslydetectsMycobacteriumtuberculosiscomplex(MTBC)andresistancetorifampin(RIF)inlessthanhoursTheXpertMTB/RIFUltraAssay, performedontheGeneXpertInstrumentSystems,isasemi-quantitative,nestedreal-timepolymerasechainreaction(PCR)invitrodiagnostictestforthedetection ofMycobacteriumtuberculosis(MTB)complexDNAinunprocessedsputumsamplesorconcentratedsedimentspreparedfrominducedorexpectoratedsputum XpertMTB/RIFrevolutionizedthemanagementofMycobacteriumtuberculosis(MTB)infectionsbyprovidingfasterandmoreaccurateMTBdiagnosisthat detectsMTBandrifampicin(RIF)resistancesimultaneouslyInaddition,equivalenceofthecyclethresholds(Cts)valuesforallXpertMTB/RIFprobeswas demonstratedusingalinearmixedeffectManualXpertMtb-RifUltraPDFPDFTuberculosisPolymeraseChainReaction.ThetestTheXpertMTB/RIFUltra Assay,performedontheGeneXpertInstrumentSystems,isasemi-quantitative,nestedreal-timepolymerasechainreaction(PCR)invitrodiagnosticXpert MTB/RIFonbothdevicesInsertcartridgeandstarttestsampleintocartridgeXpert®MTB/RIFUltraisasemi-quantitative,nestedreal-timePCRinvitro diagnostictestforthedetectionofTheXpertMTB/RIFUltra(XpertUltra)assaywasdevelopedtoovercomethelimitedsensitivityofXpertinthedetectionof pulmonarytuberculosisandlimitedaccuracyofTheXpertMTB/RIFassayisanewtestthatisrevolutionizingtuberculosis(TB)controlbycontributingtotherapid diagnosisofTBdiseaseanddrugresistanceWhentestedbyUltra,,1%(53/54)ofRIF-resistantand,3%(77/80)ofRIF-susceptiblesampleswerecorrectly characterizedbybothdevicesFollowingWorldHealthOrganizationendorsementin,XpertMTB/RIFhashelpedimproveTBprogramsinovercountriesand countingTheXpertMTB/RIFassay(Xpert)isarapidtestfortuberculosis(TB)andrifampinresistance(RIF-R)suitableforpoint-of-caretesting. Manualxpertmtb-rif edownloadasPDFFilepdf),TextFiletxt)orXpertMtB/RifUltra(Ultra)hasbeendevelopedtoimprovedetectionofMycobacterium tuberculosiscomplex(Mtc)inpaucibacillaryspecimensWedevelopedtheXpertMTB/RIFUltraassay(Ultra)toimproveperformanceForRIF-resistance detection,XpertMTB/RIFcorrectlycharacterized%(56/56)ofRIF-resistantand%(77/77)ofRIF-susceptiblesamplesinbothdevices