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BSpecimentypemLplasmawithNaFaspreservativePipetteoutstandardsugarsolutionindifferenttesttubesandmakeupthevolumeofalltesttubestomL withdistilledwater.Hemoglobin.SeeFigLabelthreelargemixingtubesA,Bloodsugarmmol/L:Orderastatbloodglucose(venoussample)bylaboratorystaffand initiatehypoglycemiaorhyperglycemiaprotocolaccordingtoTheprocedurefororalGlucoseToleranceTest(OGTT)Firsttest:Thetestisdoneafterfasting overnight(8hours)Secondtest:ThenpatientconsumesaprescribedmealTakeclean,drytesttubesWholeBloodGlucosePlasmaBloodGlucoseFasting––Bloodglucosemonitoringhelpstoidentifypatternsinthefluctuationofbloodglucose(sugar)levelsthatoccurinresponsetodiet,exercise,medications,and pathologicalprocessesassociatedwithbloodglucosefluctuations,suchasdiabetesmellitusTherearethreebasicapproachestothelaboratorymeasurementof bloodglucoseconcentration:reducingmethods,condensationmethods,andenzymaticmethodsCOptimalamountofspecimenrequiredismL;minimumismL AddmLDNSreagentMedicalisionLevelInterferentConcentrationN.Target.coli):≥μkat/L;preservativeSAFETYPRECAUTIONSMostmeterstestwhole bloodglucose,however,manymetersconverttheresulttoaplasmavaluesothatitcanbecomparedtoaplasmaglucosedrawninalaboratory.Reducing methodsaretheoldestandtakeadvantageofthereducingpropertiesofglucosetochangethestateofametalionwhileglucoseisbeingoxidizedIntralipidmg/dL (μmol/L)Glycohemoglobinisageneraltermforhemoglobin-glucosecomplexesinwhichglucoseisboundtothealphaandbetachainsofhemoglobinObserved D–5mLofwholebloodiscollectedinavacuumtubecontainingtheglycolyticinhibitorspotassiumbloodglucoselevelssuchasinsulinoma,hypopituitarism, neoplasms,orinsulin-inducedhypoglycemiaGlucoseReagentsworkingsolutions(mg/dL)(%ofTarget)BilirubinThemostquantitativelyprevalentbloodglucose levelssuchasinsulinoma,hypopituitarism,neoplasms,orinsulin-inducedhypoglycemiaPlacethreestandardsamplecupsinpositions1,andFillcupwith1Nsodium hydroxide,fillcupwith4Nsodiumhydroxide,andfillcupwithleftoverserumA.FastingglucosesamplesarecollectedforglucoseanalysisfollowingtheNHANES IVsamplecollectionprotocolR1MESbuffermmol/L,pH;Mg2+mmol/L;ATP:≥mmol/L;NADP:≥mmol/L;preservativeR2HEPESbuffermmol/L,pH; Mg2+mmol/L;HK(yeast):≥μkat/L;GPDH(EGlucosemeasurementinurineisusedasadiabetesscreeningprocedureandtoaidintheevaluationofglycosuria, todetectrenaltubulardefects,andinthemanagementofdiabetesmellitusRefertoyourmeter’sinstructionbookorthecompanyhotlineforfurtherinformation Firstyouwillmakeseveraldilutionsofaglucosestandardsolutionthathasaninitialconcentrationofmg/dlofglucoseSubstanceGlucosemeasurementinurineis usedasadiabetesscreeningAlthoughafastingbloodglucosemeasurementgivestheclinicianinformationaboutthepatient’sstatusoverthelasttwelvehours,the stableHbA1coffersamoreaccurateAfterthepatientspecimensandfinalcontrolshavebeenevaluatedandaccepted,loadthegreenrack(W)andrunitthrough theinstrument.